• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.21-26
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• 2000

A microarrayer system was developed mainly for manufacturing DNA chips. The 3-axis robot was designed to automatically collect samples from 96-or 384-well microtiter plates using up to 16 simultaneously moving pens and to deposit them on a surface-modified slide glass. This is followed by a wash/dry operation in a clean station. The cycle is repeated with a new set of samples, This system can deposit cDNA or oligonucleotides with spot intervals of $150{\;}\mu\textrm{m}$ and the spot size of $80\mu\textrm{m}$, thus allowing a high density DNA chip containing about 5,000 spots per $\textrm{cm}^2$. The entire procedure is controlled by the Visual C++ program that was written in our laboratory by using a personal computer with Pentium 100 CPU.

• 식물병연구
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• 제28권2호
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• pp.57-60
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• 2022

Leaves of Chionanthus retusus were found to be damaged by leaf spot disease associated with a fungus in Iksan, Korea. Leaf spots were angular to irregular, vein-limited, scattered, 1-8 mm diameter, brownish-gray to dark brown when dry, with heavy fructification. The pathogen causes premature defoliation of C. retusus plant and was identified as Pseudocercospora chionanthi-retusi based on morphological and molecular-phylogenetic analyses. The phylogenetic tree was constructed using multi-locus DNA sequence data of partial actin (actA), partial translation elongation factor 1-alfa (tef1), partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes, and internal transcribed spacer regions. Current study provides detail morphological description of P. chionanthi-retusi on C. retusus in Korea, with supports of phylogenetic analysis and pathogenicity test.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.51.1-51.1
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• 2005

• 한국연소학회:학술대회논문집
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• 한국연소학회 2004년도 제29회 KOSCI SYMPOSIUM 논문집
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• pp.41-45
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• 2004

A dust injection system was developed for the lime calcining rotary kiln for the coke dust from the coke dry quenching(CDQ) facility to be used as a fuel. The CDQ dust was injected with the gaseous fuel through the hole in the burner. In order to prevent the spot heating large particles should be removed from dust and dust should be injected as fast as possible so that particle combustion lasts as long as possible without precipitation. This is especially necessary when dust is burned together with gaseous fuel because the gaseous fuel can not go so far and in addition dust combustion aggravates hot spot heating. In this research a rotation drum screen was used to remove particles with diameter larger than 4mm and dust injection speed was 40m/sec. And the burner was adjusted not to use swirl that hinders flame go far away. With these measures scale generation iside the kiln could be reduced to be negligible and in addition NOx emission could be reduced from 150ppm to 20ppm. The fuel reduction was about 85Mcal/T-lime.

• Mycobiology
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• 제45권1호
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• pp.39-43
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• 2017

Leaf spot disease on black chokeberry (Aronia melanocarpa) was observed at several locations in Korea during 2014-2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola. Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally.

• 식물병연구
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• 제13권1호
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• pp.66-70
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• 2007

1997년과 1998년 광주와 광명의 상추 재배 온실에서 새로운 병해가 발견되었다. 처음에는 잎에 병반이 불규칙적이고 작은 연녹색이었다가 점점 커져 지름이 2$\sim$5 mm 정도인 수침상의 검은색 병반이 형성되었다. 잎의 아래쪽에서 엽맥을 따라 검은색의 줄무늬 병반이 형성되기도 하고 심해지면 잎이 시들어 말라 죽었다. YDC 배지에서 병원세균을 순수 분리하였을 때 Xanthomonas속 세균의 전형적인 특징인 노란색 색소를 띤 세균이 형성되었다. 분리된 세균을 $10^8$ cfu/ml로 현탁한 후 상추의 엽맥에 주사 접종하여 병원성을 확인하였다. 지방산 조성 및 함량과 다양한 탄소원 이용정도를 이용하여 분리세균 SL0246과 SL1352를 X. campestris pv. vitians로 동정하였으며 이 병을 상추의 세균성점무의병으로 명명하였다.

• 한국건축시공학회지
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• 제2권3호
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• pp.163-170
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• 2002

Recently, polishing tile(porcelain homogeneous polished tile) was used in the construction field as a finishing material. But, there happened some problems such as tile exfoliation by construction condition in early ages. Also, for use of polishing tile in the dry wall system which used to lightweight wall, the examination of adhesive stability of polishing tile is needed. In this study, adhesive strength of Polishing tile was investigated by tile bond types on gypsum board and non asbestos board coated by tar-urethane and Polymer modified cementitious waterproofing membrane(Series I). Then, the effect of heat stress and vibration was estimated on gypsum and non asbestos board(Series II). As the result of study are the follows; (1) Polishing tile(600$\times$400mm) construction on waterproofing layer : Both laboratory estimation and spot examination sieve were happened that fall of tile because their hardening speed is late. (2) To using powder style adhesives in the dry wail with waterproofing layer : Adhesive strength of tile is Influenced by interface bond area and base side condition. (3) Shock and heat stresses : obvious decline of adhesive strength is not happened

• 한국지리정보학회지
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• 제11권4호
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• pp.94-100
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• 2008

기후 시스템에서 지구온난화는 세계적으로 매우 중요한 문제이고 이는 기후변화, 이상기온, 폭우, 가뭄 등의 문제를 초래한다. 특히 가뭄은 기후변화에 의해 여러 해 동안 진행되어온 사막화를 가속화시킨다. 본 연구의 목적은 중국과 몽골 사막주변의 식생수분상태를 탐지하는 것이다. 본 연구에서는 중국과 몽골 사막 주변의 식생수분지수를 산출하기 위해 1999년부터 2006년까지의 SPOT/VEGETATION 위성 이미지를 이용하여 정규수분지수(NDWI: Normalized Difference Water Index)를 산출하였다. 건조한 상태의 식생은 사막화되기 쉽기 때문에 식생 수분은 사막화의 중요한 지표이다. SPOT/VEGETATION 위성영상의 근적외밴드(NIR)와 단파적외밴드(SWIR)의 밴드간 연산을 통하여 NDWI를 구하여 식생의 수분입자를 측정하였다. 그 결과 1999년부터 2006년까지의 NDWI는 사막주변영역에서 감소하는 경향을 보였고, 그 영역은 몽골 고비사막 북동지역과 중국 타클라마칸 사막의 남동지역에 위치해 있었다.

• 한국컴퓨터정보학회논문지
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• 제26권7호
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• pp.127-132
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• 2021

본 논문은 건화물시장과 탱커시장의 운임지수 예측에 관하여 머신러닝을 적용하였으며 신호분해법인 웨이블릿 분해와 EMD분해를 데이터 전처리 과정에 반영하여 시간의 영역의 정보와 주파수 영역의 정보를 모두 반영할 수 있는 운임예측모형을 구축하였다. 건화물 시장의 경우 웨이블릿으로 분해한 예측모형이 우수하였으며 탱커시장의 EMD분해로 예측한 모형이 우수하였으며 실무적으로 각 운송시장 참여자들에게 새로운 단기예측 방법론을 제시하였다. 이러한 연구는 운송시장에서 양적으로 가장 중요한 건화물 시장과 탱커시장에 대한 다양한 예측방법론을 확대하고 새로운 방법론을 제시하였다는 측면에서 중요하며, 변동성이 큰 운임시장에서 과학적인 의사결정 방법에 대한 실무적인 요구를 반영할 수 있을 뿐만 아니라 가장 빈번한 스팟거래에 합리적인 의사결정이 이뤄질 수 있는 기초가 될 것으로 기대된다.

• 한국환경과학회지
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• 제14권1호
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• pp.23-32
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• 2005

Surface sediments (0$\~$4 cm) were sampled at 40 stations from Ulsan Bay and its adjacent areas of Korea, to evaluate the contamination by sewage-derived organic matters using fecal sterols. Some sterols were quantified by gas chromatography coupled to mass spectrometry (GC/MS). Total concentrations of eight sterols (co­prostanol, cholesterol, cholestanol, epicholestanol, epicoprostanol, ${\beta}-sitosterol,$ brassicasterol, and stigmasterol) in the sediments from Ulsan Bay and its vicinity varied from 929 to 23,444 ng/g dry weight. The most predom­inant sterols were cholesterol and coprostanol, accounting for $33\~72{\%}$ of total sterols. The concentration of coprostanol known as the indicator of human feces ranged from 141 to 8,257 ng/g dry weight. In particular, the coprostanol concentrations in the sediments from Jansaengpo Harbor and Taehwa River exceeded the value of 1,000 ng/g dry weight, suggesting that these areas could be considered as the hot-spot zones by municipal sewage contamination. Some molecular indices and multivariate data analysis were used to assess the origin of these sterols in the sediments. Results showed that the major routes of sewage contamination in Ulsan Bay and its vicinity were the input through Jansaengpo Harbor and Taehwa River.