• 한국의류학회지
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• 제23권7호
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• pp.953-964
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• 1999

The purpose of this study was to analyze the effectiveness of negative appeal ads taking social problems as their themes. Two social problem ad themes concerning abortion and drug addiction were selected as stimulus. Questionnaires consisted of questions about affective response cognitive evaluation consumer's characteristics(sex, clothing involvement social problem involvement) and the ad and brand attitudes They were distributed to 200 high school students in Seoul. Results were : 1 The affective response consisted of 4 dimensions(negative inactivating activating positive) and the cognitive evaluation had 3 dimensions(utility·persuasive power creativity awareness) 2. Creativity and awareness dimensions and the ad attitude had positive influence on the brand attitude for the abortion theme ad,. Creativity dimension and consumer's clothing involvement had positive influence on the brand attitude for the drug addiction theme ad . Especially the affective response had no significant influence on the brand attitude. This result suggests that in case of negative appeal ads the affective response does not necessarily degrade the brand attitude while positive cognitive evaluation on creativity and awareness of the could influence the brand attitude favorably through raising attention to the brand resulting in high effectiveness of the ad.

• 한국방재안전학회논문집
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• 제8권2호
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• pp.27-31
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• 2015

본 연구의 목적은 식품 및 의료제품의 위기상황 발생 시 신속하고 효과적으로 대응할 수 있는 공무원의 역량을 향상시키기 위한 것이다. 위기대응 역량을 향상시키기 위해서는 식품 및 의료제품 위기발생시 필요한 대응활동과 필요한 역량이 무엇인지를 파악하는 것이 선행되어야 한다. 이를 위해 본 연구에서는 식의약 관련 위기에 대한 정의를 살펴보고, Emergency, Crisis 등과 관련된 역량에 대한 국내외 선행연구 및 자료를 조사 분석하였다. 분석결과를 토대로 위기 시 대응역량을 '위기 시 조치사항을 신속하게 수행하는 능력'으로 정의하고, 식약처 위기매뉴얼을 분석하여 조치사항을 도출하였다. 조치사항을 명확히 도출하는 것은 교육훈련 및 연습과 평가 환류의 기준이 되어 직접적이면서도 실질적인 역량을 제고하는데 기여할 것으로 기대된다.

• Laboraroty Animal Research
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• 제34권4호
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• pp.248-256
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• 2018

O-2-$^{18}F$-fluoroethyl-l-tyrosine ($[^{18}F]FET$) has been widely used for glioblastomas (GBM) in clinical practice, although evaluation of its applicability in non-clinical research is still lacking. The objective of this study was to examine the value of $[^{18}F]FET$ for treatment evaluation and prognosis prediction of anti-angiogenic drug in an orthotopic mouse model of GBM. Human U87MG cells were implanted into nude mice and then bevacizumab, a representative anti-angiogenic drug, was administered. We monitored the effect of anti-angiogenic agents using multiple imaging modalities, including bioluminescence imaging (BLI), magnetic resonance imaging (MRI), and positron emission tomography-computed tomography (PET/CT). Among these imaging methods analyzed, only $[^{18}F]FET$ uptake showed a statistically significant decrease in the treatment group compared to the control group (P=0.02 and P=0.03 at 5 and 20 mg/kg, respectively). This indicates that $[^{18}F]FET$ PET is a sensitive method to monitor the response of GBM bearing mice to anti-angiogenic drug. Moreover, $[^{18}F]FET$ uptake was confirmed to be a significant parameter for predicting the prognosis of anti-angiogenic drug (P=0.041 and P=0.007, on Days 7 and 12, respectively, on Pearson's correlation; P=0.048 and P=0.030, on Days 7 and 12, respectively, on Cox regression analysis). However, results of BLI or MRI were not significantly associated with survival time. In conclusion, this study suggests that $[^{18}F]FET$ PET imaging is a pertinent imaging modality for sensitive monitoring and accurate prediction of treatment response to anti-angiogenic agents in an orthotopic model of GBM.

• Molecules and Cells
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• 제38권6호
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• pp.562-572
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• 2015

We previously reported the role of histone deacetylase 3 (HDAC3) in response to anti-cancer drugs. The decreased expression of HDAC3 in anti-cancer drug-resistant cancer cell line is responsible for the resistance to anti-cancer drugs. In this study, we investigated molecular mechanisms associated with regulation of HDAC3 expression. MG132, an inhibitor of proteasomal degradation, induced the expression of HDAC3 in various anti-cancer drug-resistant cancer cell lines. Ubiquitination of HDAC3 was observed in various anti-cancer drug-resistant cancer cell lines. HDAC3 showed an interaction with SIAH2, an ubiquitin E3 ligase, that has increased expression in various anti-cancer drug-resistant cancer cell lines. miRNA array analysis showed the decreased expression of miR-335 in these cells. Targetscan analysis predicted the binding of miR-335 to the 3'-UTR of SIAH2. miR-335-mediated increased sensitivity to anti-cancer drugs was associated with its effect on HDAC3 and SIAH2 expression. miR-335 exerted apoptotic effects and inhibited ubiquitination of HDAC3 in anti-cancer drug-resistant cancer cell lines. miR-335 negatively regulated the invasion, migration, and growth rate of cancer cells. The mouse xenograft model showed that miR-335 negatively regulated the tumorigenic potential of cancer cells. The down-regulation of SIAH2 conferred sensitivity to anti-cancer drugs. The results of the study indicated that the miR-335/SIAH2/HDAC3 axis regulates the response to anti-cancer drugs.

• Molecules and Cells
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• 제39권3호
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• pp.229-241
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• 2016

We have previously reported the role of miR-326-HDAC3 loop in anti-cancer drug-resistance. CAGE, a cancer/testis antigen, regulates the response to anti-cancer drug-resistance by forming a negative feedback loop with miR-200b. Studies investigating the relationship between CAGE and HDAC3 revealed that HDAC3 negatively regulated the expression of CAGE. ChIP assays demonstrated the binding of HDAC3 to the promoter sequences of CAGE. However, CAGE did not affect the expression of HDAC3. We also found that EGFR signaling regulated the expressions of HDAC3 and CAGE. Anti-cancer drug-resistant cancer cell lines show an increased expression of $pEGFR^{Y845}$. HDAC3 was found to negatively regulate the expression of $pEGFR^{Y845}$. CAGE showed an interaction and co-localization with EGFR. It was seen that miR-326, a negative regulator of HDAC3, regulated the expression of CAGE, $pEGFR^{Y845}$, and the interaction between CAGE and EGFR. miR-326 inhibitor induced the binding of HDAC3 to the promoter sequences in anti-cancer drug-resistant $Malme3M^R$ cells, decreasing the tumorigenic potential of $Malme3M^R$ cells in a manner associated with its effect on the expression of HDAC3, CAGE and $pEGFR^{Y845}$. The down-regulation of HDAC3 enhanced the tumorigenic, angiogenic and invasion potential of the anti-cancer drug-sensitive Malme3M cells in CAGE-dependent manner. Studies revealed that $PKC{\delta}$ was responsible for the increased expression of $pEGFR^{Y845}$ and CAGE in $Malme3M^R$ cells. CAGE showed an interaction with $PKC{\delta}$ in $Malme3M^R$ cells. Our results show that HDAC3-CAGE axis can be employed as a target for overcoming resistance to EGFR inhibitors.

• 보건교육건강증진학회지
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• 제11권2호
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• pp.57-67
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• 1994

The aim of this study was to research and compare the demographic characteristics of drug abusers with non-drug abusers among junior high school students in Japan through a closed format questionnaire. The same questionnaire and face-to-face interviews were used in order to find the circumstances of drug abuser among Kyogoin(a sort of child welfare institution) students in Japan. The goal of the study was to provide basic materials for preventive education of drug abuse through the two investigations mentioned above. Between July 1993 and November 1993, the information for this study was collected from 964 students from 4 junior high schools, and also 142 students from 3 Kyogoin in Japan. A total of 1106 questionnaires were completed resulting in a following response rate of 90.4%. Information was based on the scales : family relation scale, school life scale, recognition on danger of drug abuse scale, family environment scale (Moos, 1986), self esteem inventory (Coopersmith, 1967), etc. The conclusions can be summarized as follows : 1. Drug abusers are more likely to lake communication in their families and have poorer human relations than non-drug abusers. Also their school life scores tended to be lawer non-drug abusers. 2. It was between their 6th year of elementary school and their first of Junior high school when the drug was first used. The drug of choice which they made their first attempt at using was a volatile solvent which was inhaled. It is likely that this drug is "gateway-drug" for adolescents in Japan because they then also tried other drugs (e.g. cocaine, marijuana, etc.) step by step. 3. It is therefore clearly important that greatly increasing education on the harmful effects of drug abuse before the summer vacation of the first term of the sixth year of elementary school. At the same time, intervention in the family will have an effective prevention strategy in Japan, as well., as well.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.75-75
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• 2003

Silica has been known to be a factor in acute cell injury and chronic pulmonary fibrosis. In Rat2 fibroblasts, silica induced the activation of NFkB, which plays a crucial role in regulating the expression of many genes involved in the subsequent inflammatory response. In addition, we observed that TAK1 and NIK were involved in silica-mediated NF-kB activation in Rat2 cells. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.132.1-132.1
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• 2003

Novel trans-ditrifluoroacetato, malonato-1, 4-butanediamine Pt(IV) complex, K104 was synthesized as a chemotherpeutic. The cytotoxicity of K104 against various human cancer cell lines were evaluated by MTS assay in vitro. The IC50 values of K104 ranged 15.83-25.83 $\mu\textrm{M}$, compared to CBCDA ranged 23.24-69.6 $\mu\textrm{M}$. Among several cancer cell lines, K104 showed more potent than CBCDA in colon cancer cell lines. (omitted)

• KSBB Journal
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• 제26권2호
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• pp.157-164
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• 2011

Five assays for anti-HBs were compared to improve potency test of Human lgG preparations. The three commercial EIA kits were optimized including dose response curve ranges and compared by conducting a co-laboratory study. After selecting the most reproducible EIA kit, methods comparison was performed with 22 samples in 5 different days. As a result, EIA (7.7 ${\pm}$ 5.3%) and MEIA (AxSYM: 3.7 ${\pm}$ 1.9%, IMx: 1.6 ${\pm}$ 0.8%) showed precision and accuracy (100.1 ${\pm}$ 12.6%). Therefore, the validated EIA assay was established and it is believed to be comparable to current MEIA.

• Communications for Statistical Applications and Methods
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• 제11권3호
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• pp.597-607
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• 2004

The primary interest of drug development studies is identifying the lowest dose level producing a desirable effect over that of the zero-dose control, which is referred as the minimum effective dose (MED). In this paper, we suggest a nonparametric procedure for identifying the MED with binary or ordered categorical response data. Proposed test and Williams' test are compared by Monte Carlo simulation study and discussed.