• Title/Summary/Keyword: Draft genome sequence

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Draft Genome Sequence of a Chitinase-producing Biocontrol Bacterium Serratia sp. C-1

  • Park, Seur Kee;Kim, Young Cheol
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.222-226
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    • 2015
  • The chitinase-producing bacterial strain C-1 is one of the key chitinase-producing biocontrol agents used for effective bioformulations for biological control. These bioformulations are mixed cultures of various chitinolytic bacteria. However, the precise identification, biocontrol activity, and the underlying mechanisms of the strain C-1 have not been investigated so far. Therefore, we evaluated in planta biocontrol efficacies of C-1 and determined the draft genome sequence of the strain in this study. The bacterial C-1 strain was identified as a novel Serratia sp. by a phylogenic analysis of its 16S rRNA sequence. The Serratia sp. C-1 bacterial cultures showed strong in planta biocontrol efficacies against some major phytopathogenic fungal diseases. The draft genome sequence of Serratia sp. C-1 indicated that the C-1 strain is a novel strain harboring a subset of genes that may be involved in its biocontrol activities.

Draft Genome Sequence of the Neodothiora populina-Like Yeast Strain JAF-11, Which Produces the Biosurfactant myo-Inositol Lipids

  • Jeong-Seon Kim;Parthiban Subramanian;Seunghwan Kim;Jun Heo;Bong-Sik Yun;Yiseul Kim
    • Microbiology and Biotechnology Letters
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    • v.51 no.3
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    • pp.328-331
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    • 2023
  • Genomic information of biotechnologically and industrially important microorganisms provides the basis for understanding their metabolic potential. Here, we report the draft genome sequence of the Neodothiora populina-like yeast strain JAF-11 capable of producing biosurfactant myo-inositol lipids. The draft genome contained genes associated with secondary metabolite biosynthesis, including transport and metabolism of lipids, which are a major component of fungal surfactants. Identification of myo-inositol and acyl chain synthesis genes in the draft genome corresponded to the specific biosurfactant produced by strain JAF-11. Further experimental studies could help to elucidate the genes responsible for the production of biosurfactant by strain JAF-11.

Draft genome sequence of Pelagicola sp. DSW4-44 isolated from seawater (해수에서 분리된 Pelagicola sp. DSW4-44의 초안 유전체 서열분석)

  • Oh, Ji-Sung;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.283-285
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    • 2019
  • The draft genome sequencing for Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261), isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.85 Mbp with G + C content of 54.3%, and included a total of 4,566 protein-coding genes, 3 rRNA genes, 48 tRNA genes, 3 non-coding RNA genes, and 67 pseudo genes. In the draft genome, the strain DSW4-44 contained genes involved in the nitrogen metabolism of dissimilatory nitrate reduction to ammonium (DNRA) and denitrification, which were not found other strains in the genus Pelagicola.

Draft Genome Sequence of a Chitinase-Producing Biocontrol Bacterium, Lysobacter antibioticus HS124

  • Gardener, Brian B. McSpadden;Kim, In Seon;Kim, Kil Yong;Kim, Young Cheol
    • Research in Plant Disease
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    • v.20 no.3
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    • pp.216-218
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    • 2014
  • Lysobacter antibiocus HS124 is a chitinase-producing rhizobacterium with proven capacities to suppress plant diseases. Bacterial cultures of L. antibioticus HS124 showed strong biocontrol efficacies against various plant diseases compared to those of bacterial cultures of Bacillus subtilis QST713 which is an active ingredient of a commercial biopesticide, Serenade. Here, we report the draft genome sequence and automated annotation of strain HS124. This draft genome sequence indicates the novelty of L. antibiocus HS124 and a subset of gene functions that may be related to its biocontrol activities.

Draft genome sequence of Pseudoalteromonas sp. meg-B1 isolated from marine sediment (해양퇴적물로부터 분리된 Pseudoalteromonas sp. meg-B1의 유전체 분석)

  • Park, Soo-Je;Park, Sewook
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.280-282
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    • 2018
  • Pseudoalteromonas sp. meg-B1 belonging to Gammaproteobacteria was isolated from marine sediment in Jeju island. Here, we report the draft genome sequence of strain meg-B1 with a size of approximately 4.15 Mbp and a mean G + C content of 41.2%. The draft genome included 3,606 coding sequences, and 9 ribosomal RNA and 94 transfer RNA genes. In the draft genome, genes (e.g. choline dehydrogenase) involved in the accumulation of compatible solutes required for survival in marine environments have been identified.

Draft genome sequence of Streptococcus sp. strain NM isolated from head and neck cancer patients (두경부암 환자로부터 분리된 Streptococcus sp. strain NM의 유전체분석)

  • Kim, Young Suk;Do, Kyoung-Tag;Park, Soo-Je
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.61-63
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    • 2019
  • Streptococcus sp. strain NM belonging to Firmicutes was isolated from head and neck cancer patients. Here, we report the draft genome sequence of strain NM with a size of approximately 1.90 Mbp and a mean G+C content of 39.3%. The draft genome included 1,845 coding sequences, and 12 ribosomal RNA and 58 transfer RNA genes. In the draft genome, genes involved in the antimicrobial resistance, hemolysis and defense system have been identified.

Draft Genome Sequence of Weissella koreensis Strain HJ, a Probiotic Bacterium Isolated from Kimchi

  • Seung-Min Yang;Eiseul Kim;So-Yun Lee;Soyeong Mun;Hae Choon Chang;Hae-Yeong Kim
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.128-131
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    • 2023
  • Here we report the draft genome sequence of Weissella koreensis strain HJ and genomic analysis of its key features. The genome consists of 1,427,571 bp with a GC content of 35.5%, and comprises 1,376 coding genes. In silico analysis revealed the absence of pathogenic factors within the genome. The genome harbors several genes that play an important role in the survival of the gastrointestinal tract. In addition, a type III polyketide synthase cluster was identified. Pangenome analysis identified 68 unique genes in W. koreensis strain HJ. The genome information of this strain provides the basis for understanding its probiotic properties.

Draft genome sequence of Zhongshania marina DSW25-10T isolated from seawater (해수에서 분리된 Zhongshania marina DSW25-10T 의 유전체 서열분석)

  • Oh, Ji-Sung;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.480-482
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    • 2018
  • The draft genome sequencing for Zhongshania marina $DSW25-10^T$, isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.08 Mbp with G + C content of 49.0%, and included a total of 3,702 protein-coding genes, 3 rRNA genes, 39 tRNA genes, 4 non-coding RNA genes, and 36 pseudogenes. In addition, the metabolic pathways of aliphatic and aromatic compounds were identified. In light of these metabolic pathways, Zhongshania marina $DSW25-10^T$ is expected to be a useful bioremediation resource.

Draft genome sequence of lytic bacteriophage CP3 infecting anaerobic bacterial pathogen Clostridium perfringens (혐기성 병원균 Clostridium perfringens를 감염시키는 용균 박테리오파지 CP3의 유전체 염기서열 초안)

  • Kim, Youngju;Ko, Seyoung;Yeon, Young Eun;Le, Hoa Thi;Han, Beom Ku;Kim, Hyunil;Oh, Chang-Sik;Kim, Donghyuk
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.149-151
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    • 2018
  • Clostridium perfringens is a Gram-positive, rod-shaped, anaerobic, spore-forming pathogenic bacterium, which belongs to the Clostridiaceae family. C. perfringens causes diseases including food poisoning in vertebrates and intestinal tract of humans. Bacteriophages that can kill target bacteria specifically have been considered as one of control methods for bacterial pathogens. Here, we report a draft genome sequence of the bacteriophage CP3 effective to C. perfringens. The phage genome comprises 52,068 bp with a G + C content of 34.0%. The draft genome has 74 protein-coding genes, 29 of which have predicted functions from BLASTp analysis. Others are conserved proteins with unknown functions. No RNAs were found in the genome.

Draft genome sequence of Miniimonas arenae KCTC 19750T isolated from sea sand (해양모래로부터 분리된 Miniimonas arenae KCTC 19750T의 유전체 분석)

  • Park, Soo-Je
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.278-279
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    • 2019
  • Miniimonas arenae KCTC $19750^T$ belonging to family Beutenbergiaceae of the phylum Actinobacteria was isolated from sea sand. I report here the draft genome sequence of strain KCTC $19750^T$. The draft genome comprises a size of 3,402,690 bp, a mean G + C content of 73.6%, 2,957 coding sequences, 2 ribosomal RNA genes, and 44 transfer RNA genes. Also, we found that genes involved in osmotic stress response were identified in its genome. The availability of the genome sequences will provide a more understanding of strain KCTC $19750^T$ as a unique member of the genus Miniimonas.