DOI QR코드

DOI QR Code

Draft Genome Sequence of a Chitinase-Producing Biocontrol Bacterium, Lysobacter antibioticus HS124

  • Gardener, Brian B. McSpadden (Department of Plant Pathology, The Ohio State University, Ohio Agriculture Research and Development (OARDC)) ;
  • Kim, In Seon (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Kil Yong (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Young Cheol (Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • Received : 2014.06.12
  • Accepted : 2014.07.02
  • Published : 2014.09.30

Abstract

Lysobacter antibiocus HS124 is a chitinase-producing rhizobacterium with proven capacities to suppress plant diseases. Bacterial cultures of L. antibioticus HS124 showed strong biocontrol efficacies against various plant diseases compared to those of bacterial cultures of Bacillus subtilis QST713 which is an active ingredient of a commercial biopesticide, Serenade. Here, we report the draft genome sequence and automated annotation of strain HS124. This draft genome sequence indicates the novelty of L. antibiocus HS124 and a subset of gene functions that may be related to its biocontrol activities.

Keywords

References

  1. Aziz, R. K., Bartels, D., Best, A. A., DeJongh, M., Disz, T., Edwards, R. A., Formsma, K., Gerdes, S., Glass, E. M., Kubal, M., Meyer, F., Olsen, G. J., Olson, R., Osterman, A., Overbeek, R. A., McNeil, L. K., Paarmann, D., Paczian, T., Parrello, B., Pusch, G. D., Reich, C., Stevens, R., Vassieva, O., Vonstein, V., Wilke, A. and Zagnitko, O. 2008. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9: 75. https://doi.org/10.1186/1471-2164-9-75
  2. Kang, S. J., Lee, Y. S., Lee, S. Y., Yun, G. Y., Hong, S. H., Park, Y. S., Kim, I. S., Park, R. D. and Kim, K. Y. 2010. Biological control of diamondback moth (Plutella xylostella L.) by Lysobacter antibioticus HS124. Korean J. Soil Sci. Fert. 43: 537-544. (In Korean)
  3. Kim, J. C., Choi, G. J., Park, J. H., Kim, H. T. and Cho, K. Y. 2001. Activity against plant pathogenic fungi of phomalactone isolated from Nigrospora sphaerica. Pest Manag. Sci. 57: 554-559. https://doi.org/10.1002/ps.318
  4. Ko, H. S., Tindwa, H., Jin, R. D., Lee, Y. S., Hong, S. H., Hyun, H. N., Nam, Y. and Kim, K. Y. 2011. Investigation of siderophore production and antifungal activity against Phytophthora capsici as related to iron(III) nutrition by Lysobacter antibioticus HS124. Korean J. Soil Sci. Fert. 44: 650-656. https://doi.org/10.7745/KJSSF.2011.44.4.650
  5. Ko, H. S., Jin, R. D., Krishnan, H. B., Lee, S. and Kim, K. Y. 2009. Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes. Curr. Microbiol. 59: 608-615. https://doi.org/10.1007/s00284-009-9481-0
  6. Overbeek, R., Begley, T., Butler, R., Choudhuri, J. V., Chuang, H., Cohoon, M., de Crecy-Lagard, V., Diaz, N., Disz, T., Edwards, R., Fonstein, M., Frank, E. D., Gerdes, S., Glass, E. M., Goesmann, A., Hanson, A., Iwata-Reuyl, D., Jensen, R., Jamshidi, N., Krause, L., Jubal, M., Larsen, N., Linke, B., McHardy, A. C., Meyer, F., Neuweger, H., Olsen, G., Olson, R., Osterman, A., Portnoy, V., Pusch, G. D., Rodionov, D. A., Ruckert, C., Steiner, J., Stevens, R., Thiele, I., Vassieva, O., Ye, Y., Zagnitko, O. and Vonstein, V. 2005. The subsystems approach to genome annotation and its use in the project to annotate 1000 Genomes. Nucleic Acids Res. 33: 5691-5702. https://doi.org/10.1093/nar/gki866
  7. Park, J.-K., Lee, S.-H., Lee, J.-H., Han, S., Kang, H., Kim, J.-C., Kim, Y. C. and Brian, M. G. 2013. Sampling and selection factors that enhance the diversity of microbial collections: Application to biopesticide development. Plant Pathology J. 29: 144-153. https://doi.org/10.5423/PPJ.SI.01.2013.0015
  8. Reichenbach, H. 2006. The genus Lysobacter. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. and Stackebrandt, E., editors. Prokaryotes. New York; Springer. 939-957.
  9. Zerbino, D. R. and Birney, E. 2008. Velvet: Algorithms for de novo shore read assembly using de Bruijn graphs. Genome Res. 18: 821-829. https://doi.org/10.1101/gr.074492.107
  10. Zerbino, D. R., McEwen, G. K., Margulies, E. H. and Birney, E. 2009. Pepple and Rock Band: Heurisitic resolution of repeats and scaffolding in the Velevet short-read de novo assembler. PLoS ONE 4: E8407. https://doi.org/10.1371/journal.pone.0008407
  11. Xie, Y., Wright, S., Shen, Y. and Du, L. 2012. Bioactive natural products from Lysobacter. Nat. Prod. Rep. 29: 1277-1287. https://doi.org/10.1039/c2np20064c

Cited by

  1. Biological Control Potential of Bacillus amyloliquefaciens KB3 Isolated from the Feces of Allomyrina dichotoma Larvae vol.32, pp.3, 2016, https://doi.org/10.5423/PPJ.NT.12.2015.0274
  2. Draft Genome Sequence of a Chitinase-producing Biocontrol Bacterium Serratia sp. C-1 vol.21, pp.3, 2015, https://doi.org/10.5423/RPD.2015.21.3.222
  3. species to control phytopathogenic micro-organisms vol.124, pp.1, 2017, https://doi.org/10.1111/jam.13607