• Journal of Microbiology and Biotechnology
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• 제21권4호
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• pp.374-378
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• 2011

To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using double-crossover, for use as a screening host. The W3110 dsdA::tet strain was not able to grow in a medium containing D-serine as a sole carbon source, whereas wild-type W3110 and the complement W3110 dsdA::tet strain containing a dsdA-expression plasmid were able to grow. After introducing metagenome libraries into the screening host, a strain containing a 40-kb DNA fragment obtained from the metagenomic souce derived from a compost was selected based on its capability to grow on the agar plate containing D-serine as a sole carbon source. For identification of the genetic resource responsible for the D-serine degrading capability, transposon-${\mu}$ was randomly inserted into the 40-kb metagenome. Two strains that had lost their D-serine degrading ability were negatively selected, and the two 6-kb contigs responsible for the D-serine degrading capability were sequenced and deposited (GenBank code: HQ829474.1 and HQ829475.1). Therefore, new alternative genetic resources for D-serine dehydratase was found from the metagenomic resource, and the corresponding ORFs are discussed.

• 한국농공학회지
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• 제32권4호
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• pp.71-80
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• 1990

Model tests for the ultimate pullout resistance of anchorages and investigation of failure behaviors in cohesionless soil have been conducted. The factors affecting the anchorage are mostly the geometry of the system, and soil properties of sands. The main conclusions of the experimental work were as follows. 1. The load - displacement relationship can be a form of parabolic curve for all plates. 2. The change in ultimate pullout resistance of anchor is mostly affected by embedment ratio and size of anchor, and influenced to a lesser degree by its shape. 3. Critical embedment ratio which is defined as the failure mode changes from shallow to deep mode is increased with increasing height of anchor. 4. For a constant anchor height, as the width of anchor increases the ultimate pullout resistance also increases. However, considering the efficiency of anchor for unit area, width of anchor does not appear to have any sigrnificant contribution on increasing anchor city. 5. Anchor capacity has a linear relation to sand density for any given section and the rate of change increases as the section increases. Critical depth determining the failure patterns of anchor is decreased with a decrease of sand density. 6. With increasing inclination angle, size of anchor, and decreasing embedment ratio, the ultimate pullout resistance of anchor under inclined loading is significantly decreased. 7. The ultimate pullout resistance of double anchor, a method of improving single of anchor capacity, is influenced by the center - to - center spacing adjacent anchors. It is also found that tandem and parallel anchor rigging arrangements decrease the anchor system capacity to less than twice the single anchor capacity due to anchor interference.

• 한국언론정보학보
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• 제31권
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• pp.295-325
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• 2005

이 연구는 방송과 통신의 융합과정에서 나타나고 있는 갈등이 과거의 사업자간 갈등이나 규제기구 간 갈등 혹은 사업자와 규제기구 간의 갈등과 같은 단면적 갈등을 넘어서서 사업자간 갈등과 규제기구 간 갈등이 복합되는 <복합적 갈등>의 양상을 보이고 있음을 보여주고자 하며, 이와 같은 상황에서 갈등 관리는 어떻게 이루어지고 있는지 알아봄으로써, 향후 신매체 도입과정에서 재현될 수 있는 갈등해결을 위한 바람직한 대안을 제시하는데 연구목적을 두고 있다. 이를 위해 IPTV 도입을 둘러싼 정책갈등을 사례 분석하였다. 방법론으로는 IPTV에 대한 정부의 입장 표명 등이 이루어진 시점인 2004년 12월부터 2005년 8월까지 관련 정책주체들이 공식, 비공식적으로 발표한 문헌을 분석하였으며, 이와 함께 정책주체들 간의 갈등관계를 파악하기 위해 관련 주체 10인에 대한 심층인터뷰를 실시하였다.

• 한국응용과학기술학회지
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• 제25권4호
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• pp.459-468
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• 2008

Methyl methacrylate(MMA) was grafted onto microcrystalline cellulose(MCC) with ceric ammonium nitrate(CAN) as a redox initiator at the various conditions. The cellulose triacetate(CTA) composite films added MCC and MMA-grafted MCC powders were prepared on a glass plate. The graft yield(GY) and graft efficiency(GE) of the grafted MCC were calculated with the simple equations by the weight balance method. The double bond of C=O on the grafted MCC surfaces was confirmed by the fourier transform infrared spectroscopy with attenuated total reflection(FT-IT ATR) spectrophotometer. After grafting, the degree of crystallinity of cellulose powders was decresed by judging from x-ray diffraction(XRD) data. Scanning electron microscope(SEM) photos showed the only solvent and CAN solution could change the roughness of MCC powders and the effect of powder dispersions in composite matrix. The tensile strength of MCC/CTA composite films was decreased with increase of MCC powder contents. When 5% grafted MCC was added, the tensile strength of grafted MCC/CTA composite films was increased from 82.3 MPa to 97.2 MPa. The thermal property of powders was also analyzed by the thermogravimetric analysis(TGA).

• 한국표면공학회지
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• 제47권4호
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• pp.186-191
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• 2014

Double layer films which consisted of aluminum(Al) and magnesium(Mg) have been prepared by e-beam deposition. The structure, alloy phase, and corrosion resistance of the prepared films were investigated before and after heat treatment. The first (bottom) layer fixed with Al, and the thickness ratio between Al and Mg layers has been changed from 1 : 1 to 5 : 1, respectively. Total thickness of Al-Mg film was fixed at $3{\mu}m$. The cold-rolled steel sheet was used as a substrate. Heat treatment was fulfilled in an nitrogen atmosphere at the temperature of $400^{\circ}C$ for 2, 3 and 10 min. Surface morphology of as-deposited Al-Mg film having Mg top layer showed plate-like structure. The morphology was not changed even after heat treatment. However, cross-sectional morphology of Al-Mg films was drastically changed after heat treatment, especially for the samples heat treated for 10 min. The morphology of as-deposited films showed columnar structure, while featureless structure of the films appeared after heat treatment. The x-ray diffraction data for as-deposited Al-Mg films showed only pure Al and Mg peaks. However, Al-Mg alloy peaks such as $Al_3Mg_2$ and $Al_{12}Mg_{17}$ appeared after heat treatment of the films. It is believed that the formation of Al-Mg alloy phase affected the structure change of Al-Mg film. It was found that the corrosion resistance of Al-Mg film was increased after heat treatment.

• 한국운동역학회지
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• 제22권1호
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• pp.55-63
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• 2012

The purpose of this study was to investigate the balance differences between skilled players and less-skilled players during Hakdariseogi motion of Keumgang Poomsae in Taekwondo. To achieve the study goal, total of 10 Taekwondo athletes; 5 skilled players(S, body mass: $67.0{\pm}5.7$ kg, height: $174.0{\pm}4.8$ cm, age: $20.0{\pm}2.0$ yrs) and 5 less-skilled players(LS, body mass: $73.0{\pm}4.9$ kg, height: $176.4{\pm}6.1$ cm, age: $20.8{\pm}1.3$ yrs) participated in this study. A three-dimensional motion analysis with 8 infrared cameras and one force plate whose sampling frequency as 30 Hz and 300 Hz, respectively, were performed. Participants' motion were divided into three phases which were preparation phase(P1), performing phase(P2) and maintaining phase(P3). The range and velocities of COP, the range and RMS of ground reaction torque and displacement between COM and center of BOS of each phase were computed. In this study, at P1 and P3 which were double and single stance, respectively, the range and M-L velocities of COP revealed significantly higher in LS compared with those of S(p<.05). At P2 which was single stance, LS indicated significantly higher in range of COP and ground reaction torque, and M-L velocities of COP than those of S(p<.05). The significantly shorter displacement between COM and center of BOS, however, was found in LS compared with that of S(p<.05). The results from our study indicated that S revealed more stable performance and a better posture control ability during performing Hakdariseogi motion.

• Journal of Welding and Joining
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• 제31권1호
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• pp.65-70
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• 2013

Finite element analysis and welding experiments were performed to evaluate deformation aspect for Flux Cored Arc Welding(FCAW) and Electro Gas Welding(EGW). Numerical researches of FCAW and EGW were performed considering the difference of number of welding pass and welding direction to arc flow. To perform the numerical study of FCAW and EGW, number of welding pass and welding direction to arc flow were considered in the finite element model. FCAW process requires multi pass and its welding direction is vertical to welding torch. On the other hand, EGW process requires single pass and its welding direction is parallel to welding torch. The difference of welding direction and heat input was considered in the finite element analysis. In FCAW process, Goldak's double ellipsoidal heat input model was adopted. In the EGW process, Hemi-spherical power density distribution was adopted. In the results of experiment and finite element analysis, angular deformation of FCAW process is larger than that of EGW process.

• 한국의류학회지
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• 제17권3호
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• pp.380-390
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• 1993

Effect of interfacial electrical conditions on adhesion of ${\alpha}-Fe_2O_3$ particles to PET fabric and the removal of ${\alpha}-Fe_2O_3$ particles from PET fabric, were investigated as functions of pH, electrolyte and ionic strength. The ${\zeta}$ potential of PET fiber and ${\alpha}-Fe_2O_3$ particles in the electrolyte solution were measured by streaming potential and microelectrophoresis methods respectively. The potential energy of interaction between ${\alpha}-Fe_2O_3$ particles and PET fabric were calculated by using the heterocoagulation theory for a sphere-plate model. The negative ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle and PET fiber increased with pH, and then decreased certain pH and isoelectric points of ${\alpha}-Fe_2O_3$ particles and PET fiber were pH 6.5 and pH 3.5, respectively. The negative ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle and PET fiber affected by electrolytes, were relatively high with polyanion electrolytes in solutions and were low with neutral salts. However, at surfactant solution, ${\zeta}$ potential was levelled off. The influence of the ionic strength on the ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle was small but the negative ${\zeta}$ potential of PET fiber increased with the ionic strength. In the presence of anionic surfactant, the ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle and PET fiber increased regardless of solution conditions. The interaction energy between ${\alpha}-Fe_2O_3$ particle and PET fabric increased with pH. The interaction energy was relatively high with polyanion electrolytes in solution, and the influence of ionic strength on the interaction energy was small, and the effective thickness of electrical double layer increased with decreasing the ionic strength.

• 한국운동역학회지
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• 제26권1호
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• pp.101-113
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• 2016

Objective: The purpose of this study was to investigate the effect of leg length discrepancy (LLD) on the human body during gait and standing posture. Methods: The study group comprised of 17 adult participants with LLDs of <1 cm. LLDs were artificially induced to 0, 1, 2, and 3 cm. The proportion of weight distribution, shift of the mean center of pressure, and Cobb's angle were measured in the standing position. Kinematic variables such as walking and striding width and time, and the proportion of stance phase for single- and double-limb gait were measured as well. The participants were required to either stand or walk on a treadmill (Zebris FDM) with a pressure plate, and the Cobb's angle measurements were obtained from radiographs. Results: A discrepancy of 3 cm in leg length resulted in a statistically significant shift of the center of pressure in the standing position. Moreover, the Cobb angle increased as the discrepancy became larger. The step length and width of the longer (left) leg during gait statistically significantly increased when the discrepancy was 2 cm. In addition, step time was statistically significant when the discrepancy between the longer (right) and shorter (left) legs was more than 2 cm. The proportion of single-limb stance phase was statistically significant as the discrepancy became larger, especially when the discrepancy was >2 cm for the longer (right) leg and 1 cm for the shorter (right) leg. Conclusion: The study showed that LLD influenced deformations of the human body and walking.

• International Journal of Aeronautical and Space Sciences
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• 제4권1호
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• pp.63-74
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• 2003

The vortical flow of a 65-deg flat plate delta wing with a leading edge extension(LEX) was examined through off-surface visualization, 5-hole probe and hot-film measurements. The off-surface flow visualization technique used micro water droplets generated by a home-style ultrasonic humidifier and a laser beam sheet. The angles of attack ranged from 10 to 30 degrees, and the sideslip angles ranged from 0 to -15 degrees. The Reynolds number was $1.82{\times}10^5$ for the flow visualization, and $1.76{\times}10^6$ for the 5-hole probe and hot-film measurements. The comparison of the visualization photos and the flow field measurement showed that the two results were in a good agreement for the relative position and the structure of the wing and LEX vortices, even though the flow Reynolds numbers of the two results were much different. The wing vortex and the LEX vortex coil each other while maintaining a comparable strength and identity at zero sideslip. Neither a looping of the wing vortex around the strake vortex, nor the lopsided coiling of the stronger strake and the weaker wing vortices was observed. At non-zero sideslip, the downward movement of the LEX vortex when going downstream was enhanced on the windward side, and the downward and inboard movement of the LEX vortex when going downstream was suppressed on the leeward side. The counterclockwise coiling of the wing and LEX vortices was decreased significantly on the leeward side.