• 한국환경복원기술학회지
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• 제7권5호
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• pp.85-93
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• 2004

This study was carried out to find out the effect of cool-season turfgrass overseeding onto zoysiagrass(Zoysia japonica L.) for extending green color and solving traffic problem during dormant periods. Overseeding rates of cool-season turfgrasses were $10g/m^2$ of Kentucky bluegrass(KB1), $20g/m^2$of Kentucky b1uegrass(KB2), $60g/m^2$ of perennial ryegrass(PR), $80g/m^2$ of Tall fescue(TF), and $10g/m^2$ of Kentucky bluegrass + $10g/m^2$ of perennial ryegrass mixture(KB+PR) onto 2m${\times}$2m(width${\times}$length) zoysiagrass plots with 3 replications.1 to 9-sca1e of visual quality and visual color were measured after treatment, Consequently cool-season turfgrass overseeding gave a good performances of extending the green periods and enhancing the turf grass quality. KB1 and KB2 were found to be excellent performances for visual quality and visual color compared to PR, TF, KB+PR and control. KB2 showed better turf performances than KB1 within Kentucky bluegrass overseedings. Turfgrass color was kept up for 10 months by overseeding of cool-season turfgrasses(KB1, KB2, PR, TP, KB+PR) compared to about 5~6 months by zoysiagrass(Control). The visual quality and visual color of PR and TF showed good performances during a green-up period of spring, but decreasing tendencies through the summer.Percent diseases data revealed also higher ratings for PR and TF compared to for KB1 and KB2.

• ALGAE
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• 제26권2호
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• pp.181-192
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• 2011

Ballasting and deballasting of shipping vessels in foreign ports have been reported worldwide as a vector of introduction of non-native aquatic plants and animals. Recently, attention has turned to ballast water as a factor in the global increase of harmful algal blooms (HABs). Many species of microalgae, including harmful dinoflagellate species, can remain viable for months in dormant benthic stages (cysts) in ballast sediments. Over a period of four years, we surveyed ballast water and sediment of ships docked in two ports of Tampa Bay, Florida, USA. Sampling conditions encountered while sampling ballast water and sediments were vastly different between vessels. Since no single sample collection protocol could be applied, existing methods for sampling ballast were modified and new methods created to reduce time and labor necessary for the collection of high-quality, qualitative samples. Five methods were refined or developed, including one that allowed for a directed intake of water and sediments. From 63 samples, 1,633 dinoflagellate cysts and cyst-like cells were recovered. A native, cyst-forming, harmful dinoflagellate, Alexandrium balechii (Steidinger) F. J. R. Taylor, was collected, isolated, and cultured from the same vessel six months apart, indicating that ships exchanging ballast water in Tampa Bay have the potential to transport HAB species to other ports with similar ecologies, exposing them to non-native, potentially toxic blooms.

• Archives of Plastic Surgery
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• 제35권3호
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• pp.349-353
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• 2008

Purpose: There are 3 well-known surgical procedures to treat Pierre Robin sequence: tongue-lip adhesion, distraction osteogenesis of mandible, and tracheostomy. The classical tongue-lip adhesion is an effective way to keep airway. The tongue, however, becomes quite non-mobile and appears dormant until the patient could control upper airway and the adhesion can be maintained for prolonged period. Most of all, this procedure does not provide the correction of the micrognathia. Distraction osteogenesis is a good technique to correct micrognathia and to prevent tracheostomies in patients with Pierre Robin sequence. But airway keeping procedure is needed during the distraction period. The purpose of this study is to determine the usefulness of temporary tongue-lip traction during the initial period of mandibular distraction in Pierre Robin sequence patients with severe airway problems requiring operative procedure. Methods: It was a prospective study of 2 Pierre Robin sequence patients aged between 4 months and 6 months requiring surgical procedure to correct recurrent and severe pulmonary complications. Two patients underwent distraction osteogenesis of mandible. During the operation, deep one tension suture was performed to tract the tongue and lip. When the patient gained control of upper airway at the initial period of distraction and micrognathia was corrected, the traction suture was removed. Results: All patients were followed up. No patients complained severe pulmonary complications and tracheostomy could be avoided. No patients had severe pulmonary complication. The pulmonary condition of patients was good. Conclusion: In severe Pierre Robin sequence case, temporary tongue-lip traction is a good assistant method in distraction osteogenesis because this method can avoid tracheostomy.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.110-113
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• 2005

Under the condition of nutritional deprivation, actively growing cells prepare to enter $G_0$-like stationary phase. Protein modification by phosphorylation/dephosphorylation or ubiqutination contributes to transfer cells from active cell cycle to dormant stage. We show here that Psp1/Sds23, which functions in association with the 20S cyclosome/APC (1) and is essential for cell cycle progression in Schizosaccharomyces pombe (2), is phosphorylated by stress-activated MAP kinase Sty1 and protein kinase A, as well as Cdc2/cyclinB, upon entry into stationary phase. Three serines at the positions 18,333 and 391 are phosphorylated and overexpression of Psp1 mutated on these sites causes cell death in stationary phase. These modifications are required for the binding of Spufd2, a S.pombe homolog of multiubiquitin chain assembly factor E4 in ubiquitin fusion degradation pathway. Deletion of Spufd2 gene led to increase cell viability in stationary phase, indicating that S. pombe Ufd2 functions to inhibit cell growth at this stage to maintain cell viability. Moreover, Psp1 enhances the multiubiquitination function of Ufd2, suggesting that Psp1 phosphorylated by sty1 and PKA kinases is associated with the Ufd2-dependent protein degradation pathway, which is linked to stress tolerance, to maintain cell viability in the $G_0$-like stationary phase.

• Genomics & Informatics
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• 제5권4호
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• pp.174-178
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• 2007

The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3'-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate CPE sequences in a given gene by a multiplex comparison of 3'-UTR sequences between mammalian homologs. We found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while CPE-like sequences in the 3'-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation between species. Through this cross-species comparison, we also identified an alternative CPE in the 3'-UTR of tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors governing the earliest processes of mammalian embryogenesis.

• 미생물학회지
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• 제30권3호
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• pp.154-159
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• 1992

섬유소 분해능이 우수한 P. verruculosum 의 분생포자로부터 원형질체의 생성 및 재생에 대하여 조사하였다. 균의 원형질체 생성을 위한여 2-deoxy-D-glucose(2-DG) 가 첨가된 최소배지에서 분생포자를 10-12 시간 전배양 시킨후 시판 세포벽분해효소들을 작용시켜 본 결과 그중 Novozyme 234 (1%, w/v) 에서 50% 이상의 가장 좋은 원형질체 생성 수율을 보였다. 전배양 되지 않은 포자로부터는 원형질체 생성이 이루어지지 않았으며 원형질체 생성율을 향상시키기 위한 첨가테로서 2-DG 의 효과가 무첨가에 비해 2배 이상의 수율을 증가시켰다. 포자 원형질체의 재생율은 49.2% 로 동 균주의 균사체 원형질체의 재생율4.6-27.8%(삼투압 조절체로서 0.6 M magnesium sulfate) 보다 높았다. 원형질체 생성 및 재생을 위한 상투압 안정제로서는 0.6M ammonium sulfate 와0.6 M magnesium sulfate가 가장 적절하였다. 광학 형미경을 통하여 부풀린 포자로부터 원형질체의 생성과정과 두 가지 각기 다른 양상의 재생과정이 관찰되었다.

• 한국토양비료학회지
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• 제45권6호
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• pp.1211-1215
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• 2012

Terrestrial ecosystem are a strong sink of carbon. Forest ecosystem, one of them, has been expected to play an important role in climate changing process by absorbing atmospheric carbon dioxide. On the other hand, agricultural ecosystem that consists mainly of annual crops is regarded as poor contributor to carbon accumulation, because its production (carbon hydrate) is decomposed into carbon at a short period, which is emitted to the atmosphere. However, it is thought that fruit tree plays a great role in decreasing atmospheric carbon dioxide concentration, same as forest. Net ecosystem exchange of $CO_2$ (NEE) was measured to estimate carbon fixation capacity using an eddy covariance (EC) system method in 2 years from 2005 to 2006 at an apple orchard in Uiseong, Gyeongbuk. Average air temperature values were higher in 2006 than in 2005 during the dormant season, and lower by about $5^{\circ}C$ over the growing season causing visible cold injuries. Accordingly, we investigated long-term exchange of carbon to determine how much difference of carbon fixation capacity was shown between 2006 and 2005 in terms of environmental and plant variables such as NEE, leaf area index (LAI), and Albedo. NEE was $4.8Mg\;C\;ha^{-1}yr^{-1}$ in 2005 and $4.7Mg\;C\;ha^{-1}yr^{-1}$ in 2006, respectively. Low temperature after July in 2006 decreased LAI values faster than those in 2005. Meanwhile, Albedo values were higher after July in 2006 than in 2005. These results show that the low temperature after July in 2006 apparently affected apple growth.

• 원예과학기술지
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• 제30권6호
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• pp.635-641
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• 2012

In Korea, slurry composted and biofiltered (SCB) liquid fertilizer is produced through the composting and biofiltering of animal waste. An appropriate guidelines involving proper treatment of SCB liquid fertilizer on turfgrass should be considered. An experiment was conducted to determine the optimum application amount, timing, and frequency of SCB liquid fertilizer for Zoysia japonica 'Millock'. The SCB liquid fertilizer was applied in low, medium, and high amount (N at 15, 25, and 40 $g{\cdot}m^{-2}$ per year in 2010, and 10, 20, and 40 $g{\cdot}m^{-2}$ per year in 2011, respectively) and treated during the growing season or dormancy period. During the growing season, SCB liquid fertilizer was applied twice or four times. The greatest improvement in turf quality for both years was in SCB plots applied four times with N at 40 $g{\cdot}m^{-2}$ per year during the growing season (SH4). This treatment exhibited turf color retention in the fall, and enhanced clipping yield during the growing and fall seasons. SCB plots with four times during the growing season (SL4, SM4, and SH4) exhibited higher shoot density relative to the same amount of other SCB treatments. Plots treated during the dormancy period also showed a high turf color index during the next growing season in 2011. The results indicate that SCB with high amount up to N at 40 $g{\cdot}m^{-2}$ per year applied four times during the growing season and dormant application produced high turf quality and growth, and could be recommended as an optimum application guide.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.552-560
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• 2017

Quorum quenching (QQ) bacteria entrapped in a polymeric composite hydrogel (QQ medium) have been successfully applied in membrane bioreactors (MBRs) for effective biofouling control. However, in order to bring QQ technology closer to practice, the physical strength and lifetime of QQ media should be improved. In this study, enforcement of physical strength, as well as an extension of the lifetime of a previously reported QQ bacteria entrapping hollow cylinder (QQ-HC), was sought by adding a dehydration procedure following the cross-linking of the polymeric hydrogel by inorganic compounds like $Ca^{2+}$ and boric acid. Such prepared medium demonstrated enhanced physical strength possibly through an increased degree of physical cross-linking. As a result, a longer lifetime of QQ-HCs was confirmed, which led to improved biofouling mitigation performance of QQ-HC in an MBR. Furthermore, QQ-HCs stored under dehydrated condition showed higher QQ activity when the storage time lasted more than 90 days owing to enhanced cell viability. In addition, the dormant QQ activity after the dehydration step could be easily restored through reactivation with real wastewater, and the reduced weight of the dehydrated media is expected to make handling and transportation of QQ media highly convenient and economical in practice.

• 한국통신학회논문지
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• 제29권7B호
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• pp.628-640
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• 2004

이동 네트워크는 네트워크 단위로 이동하는 구조로서, 이동 라우터에 연결된 이동 노드들의 이동을 통합하여 관리한다. 이동 네트워크 내의 노드들은 이동 라우터를 통하여 인터넷과 무선 연결되며, 모든 통신이 이동 라우터를 거쳐서 이루어지므로, 이동 라우터에서의 대역폭 관리 및 신뢰성 제공이 중요하다. 또한 이동 네트워크는 이동한다는 본질적 특성에 의하여 내부의 모든 노드들에 대한 위치 관리 및 이에 따른 전력 소비 관리를 필요로 한다. 본 논문에서는 자동차나 기차와 같은 운송체에 구성되는 이동 네트워크에서의 전력 및 대역폭 자원을 효율적으로 관리하기 위한 기법을 제안한다. 제안하는 기법은 이동 네트워크가 구성되는 운송체와 승객의 이동 특성을 이용하여, 불필요한 위치 관리 신호를 생략함으로써 전력을 절약한다. 또한 다중 이동 라우터를 이용한 멀티호밍기법에 의하여 대역폭 자원을 관리한다. 제안한 전력 관리 기법을 수학적으로 분석한 결과는 정지 상태의 이동기기 뿐 아니라 활성 상태의 이동 기기 전력도 현저하게 절약할 수 있음을 보인다. 제안한 다중 이동 라우터 선택 기법에 대한 시뮬레이션 결과는, 이 기법이 기존의 단순 라우터 중복 구조에 비하여 무선 대역폭 자원을 효율적으로 활용함을 보여준다.