• BMB Reports
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• 제38권4호
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• pp.381-385
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• 2005

Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus (CoV) that was identified and molecularly characterized in 2003. Previous studies on various coronaviruses indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly and morphogenesis. It is necessary to elucidate the molecular mechanism of SARS-CoV replication and rationalize the anti-SARS therapeutic intervention. In this study, we employed an in vitro GST pull-down assay to investigate the interaction between the membrane (M) and the nucleocapsid (N) proteins. Our results show that the interaction between the M and N proteins does take place in vitro. Moreover, we provide an evidence that 12 amino acids domain (194-205) in the M protein is responsible for binding to N protein. Our work will help shed light on the molecular mechanism of the virus assembly and provide valuable information pertaining to rationalization of future anti-viral strategies.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4031-4036
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• 2012

Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(3)
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• pp.137-140
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• 2000

In this paper, we propose as new algorithm for the selection of key frames in a given video. For the selected key frames to be well defined, the selected key frames need to spread out on the whole temporal domain of the given video and guaranteed not to be duplicate. For this purpose, we take the first frame of each shot of the video as the candidate key frame to represent the video. To reduce the overall processing time, we eliminate some candidate key frames which are visually indistinct in the histogram difference. The key frames are then selected using a clustering processing based on the singly linked hierarchical tree. To make the selected key frames be distributed evenly on the whole video, the deviation and time difference between the selected key frames are used. The simulation results demonstrate that our method provides the better performance compared with previous methods.

• 한국멀티미디어학회논문지
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• 제10권9호
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• pp.1093-1105
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• 2007

본 논문에서는 XML 데이터베이스의 색인구조로 다차원 화일구조를 이용하는 다차원 타입상속 색인기법인 MD-TIX를 제안한다. 일차원 색인구조를 이용하는 기존의 XML 데이터베이스 색인기법에서는 타입상속계층과 중첩요소가 포함된 복합 형태의 질의들에 대한 처리를 잘 지원하지 못한다. MD-TIX에서는 XML 데이터베이스의 중첩요소에 대한 색인기법을 위하여 이차원 타입상속 계층 색인기법(2D-THI)을 다차원으로 확장하여 사용한다. 2D-THI는 타입상속 계층의 단순요소에 대한 색인기법으로 킷값 도메인과 타입식별자 도메인으로 구성된 이차원 도메인 공간상에서 요소들의 클러스터링을 다루는 색인기법이다. 본 논문의 MD-TIX에서는 색인된 중첩요소를 표현하는 경로상의 각 타입상속 계층마다 하나의 타입식별자 도메인을 할당하여 구성된 다차원 도메인 공간상에서 색인 엔트리들의 클러스터링을 다룬다. 따라서 HD-TIX에서는 기존의 색인기법에서 지원하기 어려운 질의의 대상 범위가 타입상속 계층상의 임의의 타입들로 제한되거나, 질의에 포함된 복합요소들의 도메인이 타입상속 계층상의 임의의 타입들로 제한되는 경우에도 잘 지원할 수 있다.

• BMB Reports
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• 제35권1호
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• pp.1-6
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• 2002

• Nuclear Engineering and Technology
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• 제55권3호
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• pp.968-980
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• 2023

The dynamic structural responses are sensitive to the time-frequency content of seismic waves, and seismic input motions in time-history analysis are usually required to be compatible with design response spectra according to nuclear codes. In order to generate spectra-compatible input motions while maintaining the intrinsic non-stationarity of seismic waves, an improved time-domain approach is proposed in this paper. To maintain the nonstationary characteristics of the given seismic waves, a new time-frequency envelope function is constructed using the Hilbert amplitude spectrum. Based on the intrinsic mode functions (IMFs) obtained from given seismic waves through variational mode decomposition, a new corrective time history is constructed to locally modify the given seismic waves. The proposed corrective time history and time-frequency envelope function are unique for each earthquake records as they are extracted from the given seismic waves. In addition, a dimension reduction iterative technique is presented herein to simultaneously superimpose corrective time histories of all the damping ratios at a specific frequency in the time domain according to optimal weights, which are found by the genetic algorithm (GA). Examples are presented to show the capability of the proposed approach in generating spectra-compatible time histories, especially in maintaining the nonstationary characteristics of seismic records. And numerical results reveal that the modified time histories generated by the proposed method can obtain similar dynamic behaviors of AP1000 nuclear power plant with the natural seismic records. Thus, the proposed method can be efficiently used in the design practices.

• Smart Structures and Systems
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• 제14권6호
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• pp.1197-1220
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• 2014

Accurate actuator tracking is critical to achieve reliable real-time hybrid simulation results for earthquake engineering research. The frequency-domain evaluation approach provides an innovative way for more quantitative post-simulation evaluation of actuator tracking errors compared with existing time domain based techniques. Utilizing the Fast Fourier Transform the approach analyzes the actuator error in terms of amplitude and phrase errors. Existing application of the approach requires using the complete length of the experimental data. To improve the computational efficiency, two techniques including data decimation and frequency decimation are analyzed to reduce the amount of data involved in the frequency-domain evaluation. The presented study aims to enhance the computational efficiency of the approach in order to utilize it for future on-line actuator tracking evaluation. Both computational simulation and laboratory experimental results are analyzed and recommendations on the two decimation factors are provided based on the findings from this study.

• BMB Reports
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• 제47권11호
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• pp.631-636
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• 2014

Aurora-A is a centrosome-localized serine/threonine kinase that is overexpressed in multiple human cancers. We previously reported an intramolecular inhibitory regulation of Aurora-A between its N-terminal regulatory domain (Nt, amino acids [aa] 1-128) and the C-terminal catalytic domain (Cd, aa 129-403). Here, we demonstrate that although both Aurora-A mutants (AurA-K250G and AurA-D294G/Y295G) lacked interactions between the Nt and Cd, they also failed to interact with Ajuba, an essential activator of Aurora-A, leading to loss of kinase activity. Additionally, overexpression of either of the mutants resulted in centrosome amplification and mitotic spindle formation defects. Both mutants were also able to cause G2/M arrest and apoptosis. These results indicate that both K250 and D294/Y295 are critical for direct interaction between Aurora-A and Ajuba and the function of the Aurora-A complex in cell cycle progression.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.758-763
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• 2012

As a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hircus) cells, a full-length cDNA was cloned (GenBank accession number JF714970) and expression pattern was analyzed. The cloned FKBP38 gene is 1,248 bp in length, containing an open reading frame (ORF) from nucleotide 13 to 1,248 which encodes 411 amino acids, and 12 nucleotides in front of the initiation codon. The full cDNA sequence shares 98% identity with cattle, 94% with horse and 90% with human. The putative amino acid sequence shows the higher homology which is 98%, 97% and 94%, correspondingly. The bioinformatics analysis showed that FKBP38 contained a FKBP_C domain, two TPR domains and a TM domain. Psite analysis suggested that the ORF encoding protein contained a leucine-zipper pattern and a Prenyl group binding site (CAAX box). Tissue-specific expression analysis was performed by semi-quantitative RT-PCR and showed that the FKBP38 expression was detected in all the tested tissues and the highest level of mRNA accumulation was detected in testis, suggesting that FKBP38 plays an important role in goat cells.

• 한국초등과학교육학회지:초등과학교육
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• 제40권1호
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• pp.22-35
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• 2021

In order to investigate the trends in science education research using AR (Augmented Reality) and derive implications for elementary science education, we analyzed 71 research articles on AR application in science education published in both Korea and abroad from 2010 to August 2020. In quantitative aspects, the number of published articles has steadily increased. For domestic researches, the number of papers targeting for elementary school students was higher than that of middle & high school students. In the research method aspects, qualitative methods were most frequently used. In particular, papers regarding the development of AR program and verification of its effectiveness were most frequently published. The researches using mixed method in domestic field were smaller in number than that of the research in abroad. There were similar trends in research targeting elementary school students. In the aspects of the contents, more researches were performed on biology and earth science areas than others. In case of researches for elementary school students, the proportion of researches on biology and earth science was even higher. Domestically the proportion of studies on the convergence of science and non-science subjects was higher than that of foreign studies. The number of researches exploring the effectiveness on 'non-scientific attitude domain', 'cognitive domain', and 'program domain' were relatively higher than that on 'inquiry & practice domain' and 'science-related attitude domain'. For types of AR contents, 'observation manipulation type' was mostly studied, followed by 'experimental activity type', and 'learning guide type'. In case of studies on elementary school students, the ratio of 'observation manipulation type' contents was higher than that of others, whereas studies on 'field problem solving type' were relatively less reported than others. In addition, studies on 'simple interaction' were most frequently reported. Particularly, there were relatively few studies on 'linear and nonlinear interactions' in domestic field. As a result of analyzing key words, we found that the key words related to the characteristics and implementation of AR frequently occurred, and the key words related to elementary education and the merits of AR had many direct connections with other key words.