• Journal of Microbiology and Biotechnology
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• v.12 no.6
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• pp.943-949
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• 2002

It has been proposed that chitin synthase 3 (CHS3)-nediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p.

• BMB Reports
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• v.50 no.5
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• pp.269-274
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• 2017

The biological activities of macrophage migration inhibitory factor (MIF) might be mediated through a classical receptor-mediated or non-classical endocytic pathway. JAB1 (C-Jun activation domain-binding protein-1) promotes the degradation of the tumor suppressor, p53, and the cyclin-dependent kinase inhibitor, p27. When MIF and JAB1 are bound to each other in various intracellular sites, MIF inhibits the positive regulatory effects of JAB1 on the activity of AP-1. The intestinal parasite, Anisakis simplex, has an immunomodulatory effect. The molecular mechanism of action of As-MIF and human JAB1 are poorly understood. In this study, As-MIF and hJAB1 were expressed and purified with high solubility. The structure of As-MIF and hJAB1 interaction was modeled by homology modeling based on the structure of Ace-MIF. This study provides evidence indicating that the MIF domain of As-MIF interacts directly with the MPN domain of hJAB1, and four structure-based mutants of As-MIF and hJAB1 disrupt the As-MIF-hJAB1 interaction.

• Geomechanics and Engineering
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• v.2 no.4
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• pp.229-252
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• 2010

In this paper, Soil-Structure Interaction (SSI) effect is investigated using a new and integrated approach. Faster solution of time dependant differential equation of motion is achieved using numerical representation of wavelet theory while dynamic Infinite Elements (IFE) concept is utilized to effectively model the unbounded soil domain. Combination of the wavelet theory with IFE concept lead to a robust, efficient and integrated technique for the solution of complex problems. A direct method for soil-structure interaction analysis in a two dimensional medium is also presented in time domain using the frequency dependent transformation matrix. This matrix which represents the far field region is constructed by assembling stiffness matrices of the frequency dependant infinite elements. It maps the problem into the time domain where the equations of motion are to be solved. Accuracy of results obtained in this study is compared to those obtained by other SSI analysis techniques. It is shown that the solution procedure discussed in this paper is reliable, efficient and less time consuming as compared to other existing concepts and procedures.

• Fibers and Polymers
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• v.2 no.2
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• pp.81-85
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• 2001

The effects of rate of phase separation to ester interchange reactions and the repulsive pair interaction energy on the phase behavior in a phase-separated immiscible polyester blend are investigated using a Monte Carlo simulation method. The time evolution of structure factor and the degree of randomness are monitored as a function of homogenization time. When the phase separation is dominant over ester interchange reactions, the domain size slowly increases with homogenization time. However, when the pair interaction becomes less repulsive, the domain size does not significantly change with homogenization time. On the other hand, when ester interchange reactions are dominant over the phase separation, the homogenization proceeds without a change in the domain size. The higher the extent of phase separation, the lower the increasing rate of the DR. However, when the phase separation is sufficiently dominant, the effect of the extent of phase separation on the increasing rate of the degree of randomness become less significant.

• Proceedings of the Korea Crystallographic Association Conference
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• 2003.05a
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• pp.14-14
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• 2003

We have crystallized and determined the structures o the Shank PDZ domain, alone and in complex with the synthetic C-terminal hexapeptide of GKAP protein at resolutions of 1.8Å and 2.5Å, respectively. The structure revealed the structural basis of the ligand recongition by Class I PDZ-ligand interaction. Moreover, dimeric structureof shank PDZ domain suggests that the βA strand is a common surface for dimerization of PDZ domains.

• Structural Engineering and Mechanics
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• v.17 no.3_4
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• pp.573-591
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• 2004

A hybrid control system is presented for seismic-resistant building structures with and without soil-structure interaction (SSI). The hybrid control is a damper-actuator-bracing control system composed of passive and active controllers. An intelligent algorithm is developed for the hybrid system, in which the passive damper is designed for minor and moderate earthquakes and the active control is designed to activate when the structural response is greater than a given threshold quantity. Thus, the external energy for active controller can be optimally utilized. In the control of a multistory building, the controller placement is determined by evaluating the optimal location index (OLI) calculated from six earthquake sources. In the study, the soil-structure interaction is considered both in frequency domain and time domain analyses. It is found that the interaction can significantly affect the control effectiveness. In the hybrid control algorithm with intelligent strategy, the working stages of passive and active controllers can be different for a building with and without considering SSI. Thus SSI is essential to be included in predicting the response history of a controlled structure.

• The Journal of the Acoustical Society of Korea
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• v.23 no.2E
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• pp.56-67
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• 2004

Despite abundant research outcomes of blind source separation (BSS) in many types of simulated environments, their performances are still not satisfactory to be applied to the real environments. The major obstacle may seem the finite filter length of the assumed mixing model and the nonlinear sensor noises. This paper presents a two-step speech enhancement method with multiple microphone inputs. The first step performs a frequency-domain BSS algorithm to produce multiple outputs without any prior knowledge of the mixed source signals. The second step further removes the remaining cross-channel interference by a spectral cancellation approach using a probabilistic source absence/presence detection technique. The desired primary source is detected every frame of the signal, and the secondary source is estimated in the power spectral domain using the other BSS output as a reference interfering source. Then the estimated secondary source is subtracted to reduce the cross-channel interference. Our experimental results show good separation enhancement performances on the real recordings of speech and music signals compared to the conventional BSS methods.

• Steel and Composite Structures
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• v.41 no.5
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• pp.761-773
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• 2021

This study presents a 3D non-linear finite element (FE) assessment of dynamic soil-structure interaction (SSI). The numerical investigation has been performed on the time domain through a Finite Element (FE) system, while considering the nonlinear behavior of soil and the multi-directional nature of genuine seismic events. Later, the FE outcomes are analyzed to the recorded in-situ free-field and structural movements, emphasizing the numerical model's great result in duplicating the observed response. In this work, the soil response is simulated using an isotropic hardening elastic-plastic hysteretic model utilizing HSsmall. It is feasible to define the non-linear cycle response from small to large strain amplitudes through this model as well as for the shift in beginning stiffness with depth that happens during cyclic loading. One of the most difficult and unexpected tasks in resolving soil-structure interaction concerns is picking an appropriate ground motion predicted across an earthquake or assessing the geometrical abnormalities in the soil waves. Furthermore, an artificial neural network (ANN) has been utilized to properly forecast the non-linear behavior of soil and its multi-directional character, which demonstrated the accuracy of the ANN based on the RMSE and R² values. The total result of this research demonstrates that complicated dynamic soil-structure interaction processes may be addressed directly by passing the significant simplifications of well-established substructure techniques.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.3039-3044
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• 2007

A few fluid structure interaction analyses have been developed for turbomachinery blades in comparison with aircraft wings. Also, the existing aeroelastic analyses for turbomachinery blades have been mostly limited to cases with a steady freestream. In reality, however, the inflowing freestream is often pulsating. Therefore, this paper presents stability and forced response analyses of an isolated three-dimensional blade under pulsating freestream conditions. A new three-dimensional unsteady vortex lattice model under a pulsating freestream has been developed in discrete time domain to examine unsteady aerodynamic forces acting on a vibrating blade. The blade's structural behaviors have been analyzed by using a three-dimensional plate model. In the aeroelastic analysis, the flutter onset of a blade under pulsating freestream is predicted by the Floquet analysis. The new time domain method can predict aeroelastic stability as well as time history.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.10a
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• pp.132-139
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• 2000

This paper presents a new hybrid approach for nonlinear dynamic analysis of the soil-structure interaction system in the time domain. It employs, in a practical manner, a linear SSI program and a general-purpose nonlinear finite element program. In order to demonstrate the validity and applicability of the proposed method, seismic response analyses are carried out for a free-field problem and a 2-D subway station. The results indicate that the proposed methodology gives reasonable solution for the linear/nonlinear SSI problem utilizing a general-purpose finite element program. Some further studies will endorse the applicability of the method to various soil-structure interaction problems.