• 제목/요약/키워드: Domain interaction

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Backbone Assignment of Phosphorylated Cytoplasmic Domain B of Mannitol Transporter IIMtl in Thermoanaerobacter Tengcongensis

  • Lee, Ko On;Suh, Jeong-Yong
    • Journal of the Korean Magnetic Resonance Society
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    • v.21 no.1
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    • pp.20-25
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    • 2017
  • The cytoplasmic domains A and B of the mannitol transporter enzyme $II^{Mtl}$ are covalently linked in Escherichia coli, but separately expressed in Thermoanaerobacter Tengcongensis. The phosphorylation of domain B ($TtIIB^{Mtl}$) substantially increases the binding affinity to the domain A ($TtIIA^{Mtl}$) in T. Tengcongensis. To understand the structural basis of the enhanced domain-domain interaction by protein phosphorylation, we obtained NMR backbone assignments of the phospho-$TtIIB^{Mtl}$ using a standard suite of triple resonance experiments. Our results will be useful to monitor chemical shift changes at the active site of phosphorylation and the binding interfaces.

Development of Analytical Two Dimensional Infinite Elements for Soil-Structure Interaction Analysis (지반-구조물의 상호작용 해석을 위한 해석적 2차원 무한요소)

  • 윤정방;김두기
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 1997.04a
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    • pp.19-26
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    • 1997
  • In this paper, two dimensional analytical infinite elements which can include multiple wave components to model a underlying half-space are developed. Since these elements are expressed clearly and simply using Legendre polynomials of frequencies in frequency domain, these are very economical and efficient in computing the responses of strip foundations in frequency domain and are easily transformed for SSI analysis in time domain. To prove the behavior of the proposed two dimensional analytical infinite elements, vertical, horizontal, and rocking compliances of a rigid strip foundation in layered soils are analyzed and compared with those of Tzong ' Penzie $n^{(17)}$ and with those which calculated by numerical infinite elemen $t^{(1)}$ in frequency domain, and good agreements are noticed between them. As an application for a further study, a new scheme for SSI analysis in time domain are proposed and verified by comparing the displacement responses of the soil with a underlying rock due to a rectangular impulse loading with those of a soil modeled extended FE meshes.soil modeled extended FE meshes.

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Structured Group Interaction System for Collaborative Problem Solving (협업 방식의 문제 해결을 위한 그룹원 간의 구조적 대화 지원 시스템)

  • 전건호;서용무
    • Korean Management Science Review
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    • v.20 no.2
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    • pp.81-93
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    • 2003
  • These days we come across many wicked problems whose solutions are beyond individuals intellectual ability. These problems can be resolved through collaborative group interaction. We developed an internet-based asynchronous group interaction support system, after looking into the collaborative problem solving process and the IBIS (Issue-Based Information System) argumentation model. It has the following characteristics ; 1) it is developed based on the modified IBIS model which is a model for group interaction to resolve wicked problems ; 2) it supports both processes of seeking and comparing solutions, while most similar systems do not have a feature to support the latter process ; 3) different structures can be defined dynamically according to the purpose of group interaction, so that it could be used for collaborative problem solving in a specific domain. To show the usability of the system, we carried out an experiment, whose result is shown at the end.

Seismic Analysis of Bridges Accounting for Soil-Pile-Structure Interaction (지반-말뚝-구조물 상호작용을 고려한 교량구조물의 지진해석)

  • Kim, Moon-Kyun;Lim, Yun-Mook;Cho, Kyung-Hwan;Kim, Ji-Sun
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2005.03a
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    • pp.405-412
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    • 2005
  • In this study, a numerical method for soil-pile-structure interaction problems in multi-layered half-plane is developed. The total soil-pile-structure interaction system is divided into two parts namely, nonlinear structure part and linear soil-pile interaction parts. In the structure field, the general finite element method is introduced to solve the dynamic equation of motion for the structure. In the soil-pile structure interaction part, physical model consisting of lumped parameter, which is frequency dependent coefficient and determined by rigorous analysis method is introduced. Using proposed analysis procedure, the nonlinear behavior of structure considering soil-structure interaction can be efficiently determined in time domain and the analysis cost is dramatically reduced.

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Interaction between IgE-Dependent Histamine-Releasing Factor and Triosephosphate Isomerase in HeLa Cells (HeLa 세포에서 IgE-dependent Histamine-Releasing Factor와 Triosephosphate Isomerase의 상호작용 규명)

  • Moon Ji-Ae;Kim Hwa-Jung;Lee Kyunglim
    • Microbiology and Biotechnology Letters
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    • v.33 no.4
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    • pp.255-259
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    • 2005
  • IgE-dependent histamine-releasing factor (HRF) is found extracellularly to regulate the degranulation process of histamine in mast cells and basophils and known to play a predominant role in the pathogenesis of chronic allergic disease. HRF has been also identified in the intracellular region of the cell. Previously, we reported that HRF interacts with the 3rd cytoplasmic domain of the alpha subunit of Na,K-ATPase. To understand the molecular mechanism of the regulation of Na, K-ATPase activity by HRF, we investigated the interaction between HRF and TPI since TPI was obtained as HRF-interacting protein in HeLa cDNA library, using yeast two hybrid screening. Domain mapping study of the interaction between HRF and TPI revealed that the C-terminal region of the residue 156-249 of TPI is involved in the interaction with HRF. The interaction between HRF and TPI was confirmed by immunoprecipitation from HeLa cell extracts. Our results suggest that TPI is a HRF-binding protein and the interaction between HRF and TPI nay thus affect Na, K-ATPase activity.

Structural Studies on the E. coli Methionyl-tRNA Synthetase and Their Interaction with E. coli $tRNA^{fMet}$

  • Kim Ji-Hun;Ahn Hee-Chul;Park Sung-Jin;Kim Sung-Hoon;Lee Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.9 no.2
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    • pp.110-121
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    • 2005
  • E.coli methionyl tRNA synthetase consist of 676 amino acids and plays a key role in initiation of protein synthesis. The native form of this enzyme is a homodimer, but the monomeric enzyme truncated approximately C-terminal 120 amino acids retains the full enzymatic activities. X-ray crystal structure of the active monomeric enzyme shows that it has two domains. The N-terminal domain is thought to be a binding site for acceptor stem of tRNA, ATP, and methionine. The C-terminal domain is mainly a-helical and makes an interaction with the anticodon of $tRNA^{Met}$. Especially it is suggested that the region of helix-loop-helix including the tryptophan residue at the position 461 may be the essential for the interaction with anticodon of $tRNA^{Met}$. In this work the structure and function of E. coli methionyl-tRNA synthetase was studied by spectroscopic method (NMR, CD, Fluorescence). The importance of tryptophan residue at the position 461 was investigated by fluorescence spectroscopy. Tryptophan 461 is expected to be an essential site for the interaction between E. coli methionyl-tRNA synthetase and E. coli $tRNA^{Met}$. Proton and heteonuclear 2-dimensional NMR spectroscopy were also used to elucidate the protein-tRNA interaction.

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LCT: A Lightweight Cross-domain Trust Model for the Mobile Distributed Environment

  • Liu, Zhiquan;Ma, Jianfeng;Jiang, Zhongyuan;Miao, Yinbin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.10 no.2
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    • pp.914-934
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    • 2016
  • In the mobile distributed environment, an entity may move across domains with great frequency. How to utilize the trust information in the previous domains and quickly establish trust relationships with others in the current domain remains a challenging issue. The classic trust models do not support cross-domain and the existing cross-domain trust models are not in a fully distributed way. This paper improves the outstanding Certified Reputation (CR) model and proposes a Lightweight Cross-domain Trust (LCT) model for the mobile distributed environment in a fully distributed way. The trust certifications, in which the trust ratings contain various trust aspects with different interest preference weights, are collected and provided by the trustees. Furthermore, three factors are comprehensively considered to ease the issue of collusion attacks and make the trust certifications more accurate. Finally, a cross-domain scenario is deployed and implemented, and the comprehensive experiments and analysis are conducted. The results demonstrate that our LCT model obviously outperforms the Bayesian Network (BN) model and the CR model in our cross-domain scenario, and significantly improves the successful interaction rates of the honest entities without increasing the risks of interacting with the malicious entities.

Moieties of Complement iC3b Recognized by the I-domain of Integrin αXβ2

  • Choi, Jeongsuk;Buyannemekh, Dolgorsuren;Nham, Sang-Uk
    • Molecules and Cells
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    • v.43 no.12
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    • pp.1023-1034
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    • 2020
  • Complement fragment iC3b serves as a major opsonin for facilitating phagocytosis via its interaction with complement receptors CR3 and CR4, also known by their leukocyte integrin family names, αMβ2 and αXβ2, respectively. Although there is general agreement that iC3b binds to the αM and αX I-domains of the respective β2-integrins, much less is known regarding the regions of iC3b contributing to the αX I-domain binding. In this study, using recombinant αX I-domain, as well as recombinant fragments of iC3b as candidate binding partners, we have identified two distinct binding moieties of iC3b for the αX I-domain. They are the C3 convertase-generated N-terminal segment of the C3b α'-chain (α'NT) and the factor I cleavage-generated N-terminal segment in the CUBf region of α-chain. Additionally, we have found that the CUBf segment is a novel binding moiety of iC3b for the αM I-domain. The CUBf segment shows about a 2-fold higher binding activity than the α'NT for αX I-domain. We also have shown the involvement of crucial acidic residues on the iC3b side of the interface and basic residues on the I-domain side.

Advanced Computational Dissipative Structural Acoustics and Fluid-Structure Interaction in Low-and Medium-Frequency Domains. Reduced-Order Models and Uncertainty Quantification

  • Ohayon, R.;Soize, C.
    • International Journal of Aeronautical and Space Sciences
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    • v.13 no.2
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    • pp.127-153
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    • 2012
  • This paper presents an advanced computational method for the prediction of the responses in the frequency domain of general linear dissipative structural-acoustic and fluid-structure systems, in the low-and medium-frequency domains and this includes uncertainty quantification. The system under consideration is constituted of a deformable dissipative structure that is coupled with an internal dissipative acoustic fluid. This includes wall acoustic impedances and it is surrounded by an infinite acoustic fluid. The system is submitted to given internal and external acoustic sources and to the prescribed mechanical forces. An efficient reduced-order computational model is constructed by using a finite element discretization for the structure and an internal acoustic fluid. The external acoustic fluid is treated by using an appropriate boundary element method in the frequency domain. All the required modeling aspects for the analysis of the medium-frequency domain have been introduced namely, a viscoelastic behavior for the structure, an appropriate dissipative model for the internal acoustic fluid that includes wall acoustic impedance and a model of uncertainty in particular for the modeling errors. This advanced computational formulation, corresponding to new extensions and complements with respect to the state-of-the-art are well adapted for the development of a new generation of software, in particular for parallel computers.

Electro-Fluid-Structural Interaction Simulation of a Valveless Micropump (시뮬레이션을 통한 무밸브 마이크로 펌프의 전기-유체-구조 상호작용에 대한 연구)

  • Li, Guang-Zhe;Goo, Nam-Seo;Han, Cheol-Heui
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.36 no.1
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    • pp.7-13
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    • 2008
  • In this paper, the pumping performance of a piezoelectric valveless micropump is simulated with a commercial finite element analysis software, COMSOL Multiphysics. The micropump developed in the previous work is composed of a 4-layer lightweight piezo-composite actuator (LIPCA), a polydimethylsiloxane (PDMS) pump chamber, and two diffusers. The piezoelectric domain, structural domain and fluid domain are coupled in the simulation. Water flow rates are numerically predicted for geometric parameters of the micropump. Based on this study, the micropump is optimally designed to obtain its highest pumping performance.