• Journal of Institute of Control, Robotics and Systems
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• v.5 no.6
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• pp.676-682
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• 1999

A study which develops a controller design methodology that has flexibility of eigenstructure assignment within the stability-robustness contraints of LQR is requried and has been performed. The previously developd control design methodology, namely, EALQR(Eigenstructure Assignment/LQR) has better performance than that of conventional LQR or eigenstructure assignment but has a constraint for the weitgting matrix in LQR, which could be indefinite for high-order system. In this paper, the effects of the indefinite Q in EALQR on the frequency domain properties are analyzed. The robustness criterion and quantitative frequency domain properties are also resented. Finally, the frequency domain properties of EALQR has been analyzed by applying to a flight control system design example.

• Journal of Mechanical Science and Technology
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• v.17 no.3
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• pp.329-335
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• 2003

EALQR (Linear Quadratic Regulate. design with Eigenstructure Assignment capability) has the capability of exact assignment of eigenstructure with the guaranteed margins of the LQR for MIMO (Multi-Input Multi-Output) systems. However, EALQR undergoes a restriction on the state-weighting matrix Q in LQR to be indefinite with respect to the region of allowable closedloop eigenvalues. The definiteness of the weighting matrix is closely related to the robustness property of a given system. In this paper, we derive a relation between the indefinite weighting matrix Q and the robustness property for EALQR. The modified frequency domain inequality, that could be guaranteed by EQLQR with an indefinite weighting matrix, is presented.

• International Journal of Control, Automation, and Systems
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• v.5 no.2
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• pp.111-116
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• 2007

The design problem of partial pole assignment (PPA) in two classes of frequency-domain MIMO models by constant gain feedback is investigated in this paper. Its aim is to design a constant gain feedback which changes only a subset of the open-loop eigenvalues, while the rest of them are kept unchanged in the closed-loop system. A near general parametric expression for the feedback gain matrix in term of a set of design parameter vectors and the set of the closed-loop poles, and a simple parametric approach for solving the proposed problem are presented. The set of poles do not need to be previously prescribed, and can be set undetermined and treated together with the set of parametric vectors as degrees of design freedom provided by the approach. An illustrative example shows that the proposed parametric method is simple and effective.

• Molecules and Cells
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• v.27 no.4
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• pp.493-496
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• 2009

Hypoxia-inducible factor $1{\alpha}$ ($HIF1{\alpha}$) is a transcription factor that plays a key role in the adaptation of cells to low oxygen stress and oxygen homeostasis. The oxygen-dependent degradation (ODD) domain of $HIF1{\alpha}$ responsible for the negative regulation of $HIF1{\alpha}$ in normoxia is intrinsically unfolded. Here, we carried out the backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignment of a proteolysis-resistant fragment (residues 404-477) in the $HIF1{\alpha}$ ODD domain using NMR spectroscopy. About 98% (344/352) of all the $^1HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$, and $^{13}CO$ resonances were unambiguously assigned. The results will be useful for further investigation of the structural and dynamic states of the $HIF1{\alpha}$ ODD domain and its interaction with binding partners.

• Journal of the Korean Magnetic Resonance Society
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• v.21 no.1
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• pp.20-25
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• 2017

The cytoplasmic domains A and B of the mannitol transporter enzyme $II^{Mtl}$ are covalently linked in Escherichia coli, but separately expressed in Thermoanaerobacter Tengcongensis. The phosphorylation of domain B ($TtIIB^{Mtl}$) substantially increases the binding affinity to the domain A ($TtIIA^{Mtl}$) in T. Tengcongensis. To understand the structural basis of the enhanced domain-domain interaction by protein phosphorylation, we obtained NMR backbone assignments of the phospho-$TtIIB^{Mtl}$ using a standard suite of triple resonance experiments. Our results will be useful to monitor chemical shift changes at the active site of phosphorylation and the binding interfaces.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.10-10
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• 2000

Eigenstructure assignment is a typical method with the capability of the consideration of the specifications in time-domain in designing a 1]near control system. In general eigenstructure assignment such that all the desired eigenvalues are exactly assigned to the closed-loop system, the assignment of the eigenvectors is very restrictive. However if the arbitrary point in a certain area as an eigenvalue is allowed to be assigned t the closed-loop system, the assignment of the eigenvector corresponding to this eigenvalue can be much less restrictive. In this paper, the flexible eigenstructure assignment that can assign more closely the desired eigenvector to the closed-loop system by using an optimization technique is proposed.

• Journal of the Korean Magnetic Resonance Society
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• v.20 no.2
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• pp.50-55
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• 2016

Backbone $^1H$, $^{13}C$ and $^{15}N$ resonance assignments are presented for the anticodon binding domain (residues 557-674) of human glycyl-tRNA synthetase (GRS). Role of the anticodon binding domain (ABD) of GRS as an anticancer ligand has recently been reported and its role in other diseases like Charcot-Marie-Tooth (CMT) and polymyositis have increased its interest. NMR assignments were completed using the isotope [$^{13}C/^{15}N$]-enriched protein and chemical shifts based secondary structure analysis with TALOS+ demonstrate similar secondary structure as reported in X-ray structure PDB 2ZT8, except some C-terminal residues. NMR signals from the N-terminal residues 557 to 571 and 590 to 614 showed very weak or no signals exhibiting dynamics or conformational exchange in NMR timescale.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.10
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• pp.6310-6316
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• 2014

The increase in the Internet and smart device users requires high-level network security. Network security consists of Web Firewall, Network Firewall, IPS, DDoS system, UTM (Unified Treat Management), VPN, NAC (Network Access Control), Wireless security, Mobile security, and Virtualization. Most network security solutions running on IPv4, and IPv6 network services are not sufficiently ready. Therefore, in this paper, this study designed and implemented important functions of Network Access Control (NAC), which include IPv6 host detection, isolation, blocking and domain assignment for the IPv6 network. In particular, domain assignment function makes 128 bits IPv6 address management easy. This system was implemented on a KISA IPv6 test-bed using well known devices. Finally, the test result showed that all IPv6 based wired and wireless devices were well-controlled (detection, blocking, isolation and domain assignment).

• Journal of the Korean Magnetic Resonance Society
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• v.28 no.2
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• pp.6-9
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• 2024

HscA is a Hsp70-type chaperone protein that plays an essential role to mediate the iron-sulfur (Fe-S) cluster biogenesis mechanism in Escherichia coli. Like other Hsp70 chaperones, HscA is composed of two domains: the nucleotide binding domain (NBD), which can hydrolyze ATP and use its chemical energy to facilitate the Fe-S cluster transfer process, and the substrate binding domain (SBD), which directly interacts with the substrate, IscU, the scaffold protein of an Fe-S cluster. In the present work, we prepared the isolated SBD construct of HscA (HscA(SBD)) and conducted the solution-state nuclear magnetic resonance (NMR) experiments to have its backbone chemical shift assignment information. Due to low spectral quality of HscA(SBD), we obtained all the NMR data from the sample containing the peptide LPPVKIHC, the HscA-interaction motif of IscU, from which the chemical shift assignment could be done successfully. We expect that this information provides an important basis to execute detailed structural characterization of HscA and appreciate its interaction with IscU.

• 제어로봇시스템학회:학술대회논문집
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• 1998.10a
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• pp.429-434
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• 1998

The previously developed control design methodology, EALQR(Eigenstructure Assignment/LQR), has better performance than that of conventional LQR or eigen-structure assignment. But it has a constraint for the weigting matrix in LQR, that is the weighting matrix could be indefinite for high-order systems. In this paper, the effects of the indefinite weighting matrix in EALQR on the Sequency domain properties are analyzed. The robustness criterion and quantitative frequency domain properties are also presented. Finally, the frequency do-main properties of EALQR has been analyzed by applying to a flight control system design example.