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Prevalence and Density of Digenetic Trematode Metacercariae in Clams and Oysters from Western Coastal Regions of the Republic of Korea

  • Sohn, Woon-Mok;Na, Byoung-Kuk;Cho, Shin-Hyeong;Lee, Won-Ja
    • Parasites, Hosts and Diseases
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    • v.55 no.4
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    • pp.399-408
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    • 2017
  • A survey was performed to know the recent infection status of digenetic trematode metacercariae in clams and oysters from 4 sites in western coastal regions of the Republic of Korea (=Korea). Four species of clams (Mactra veneriformis, Ruditapes philippinarum, Cyclina sinensis, and Saxidomus purpuratus) were collected from Taean-gun, Chungcheongnam-do (Province), Buan-gun (County) and Gochang-gun, Jeollabuk-do, and oysters, Crassostrea gigas, from Shinan-gun, Jeollanam-do were transferred to our laboratory on ice and examined by the artificial digestion method. The metacercariae of Himasthla alincia were detected in 3 species of clams, M. veneriformis, R. philippinarum, and C. sinensis from the 3 surveyed areas. The positive rate and the mean density per clam infected were 98.9% (30.8 metacercariae) in M. veneriformis, 60.0% (5.0) in R. philippinarum, and 96.0% (28.4) in C. sinensis. The positive rate (mean density) of Acanthoparyphium tyosenense metacercariae in M. veneriformis was 50.0% (2.1) from Taean-gun and 70.0% (2.8) from Gochang-gun. The metacercariae of Parvatrema spp. were detected in M. veneriformis and R. philippinarum from Taean-gun and Gochang-gun; the positive rate (mean density) was 63.3% (4,123) and 50.0% (19) in M. veneriformis, and 6.7% (126) and 100% (238) in R. philippinarum from the 2 regions, respectively. The metacercariae of Gymnophalloides seoi were detected in all 30 oysters from Shinan-gun, and their average density per oyster was 646. From the above results, it has been confirmed that more than 3 species of metacercariae are prevalent in clams from the western coastal regions, and G. seoi metacercariae are still prevalent in oysters from Shinan-gun, Jeollanam-do, Korea.

The Growth Phase and Yield Difference of Kenaf (Hibiscus cannabinus L.) on Soil Salinity in Reclaimed Land (간척지에서 토양 염농도별 케나프의 생육반응 및 수량성)

  • Kang, Chan-Ho;Choi, Weon-Young;Yoo, Young-Jin;Choi, Kyu-Hwan;Kim, Hyo-Jin;Song, Young-Ju;Kim, Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.511-520
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    • 2014
  • Kenaf (Hibiscus cannabinus L.) was recognized as a potential source of forage. To reduce the production cost, we should insure large cultivation area. The one of the best candidate places to expand the useful kenaf production was 'Saemangeum' reclaimed land. To confirm the possibility of kenaf growth in reclaimed land, we seeding and cultivated the kenaf in 'Saemangeum'. The germination percentage of kenaf on 5.0 dS/m soil salinity was 18%. It is less 66% than that of 4.0 dS/m soil salinity and at 6.0 dS/m, the germination percentage of kenaf was under 10%. The growth and development of kenaf in reclaimed land grew worse with increasing soil salinity. The stem diameter which the most important factor that decide the value and yield of product was upper 2.6 cm when soil salinity maintained under 4.0 dS/m, but if soil salinity marked over 4.0 dS/m, the stem diameter of kenaf was drop under 2.0 cm and it deteriorate the number of leaves per plant by 20~46%. The necrosis on older tip and marginal leaves were noted approximately first month after seeding which was correlated directly with the salinity levels of reclaimed soil. Reduction of total yield was coincide with increasing levels of EC. If soil salinity over 5.0 dS/m, the amount of decreased by soil salinity was 51% than that of non-reclaimed region. The allowable soil salinity level of which could be maintained within 20% reduction rate was 4.2 dS/m. Consequently kenaf can be grown successfully with moderately saline soil condition. However, salt levels in excess of 4.2 dS/m severely have restricted plant growth and development and will result in significant yield reduction.

Effects of LED(Light Emitting Diode) Photoperiod and Light Intensity on Growth and Yield of Taraxacum coreanum Nakai in a Plant Factory (식물공장 내 광주기 및 광도가 흰민들레의 생육과 수량에 미치는 영향)

  • Hwang, Yeon Hyeon;Park, Ji Eun;Chang, Young Ho;An, Jae Uk;Yoon, Hae Suk;Hong, Kwang Pyo
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.232-239
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    • 2016
  • The objective of this study was to examine the effect of photoperiod and light intensity of RBW LED (red:blue:white = 2:1:1) on the growth of Taraxacum coreanum Nakai in a fully artificial light type plant factory. 3 photoperiods and 4 light intensity were used respectively in a fully artificial light type plant production system. Plants were cultured with three photoperiods and four light intensity regimes (conditions) for 270 and 120 days, respectively, using nutrient film technique (NFT) or aeroponics culture methods. For each photoperiod, the total leaves per plant harvested 8 times in all cultivation period was 224 in the 16/8(day/light) photoperiod that had no significant difference from 220 in the 12/12 photoperiod and the lowest number of leaves was 151 occurred in the 8/16 photoperiod, which means that the longer photoperiod, the more leaves harvest. Total fresh weight of above ground was the high in order of in 16/8 photoperiod as 125g, 12/12 photoperiod as 91g, 8/16 photoperiod as 56g. For each light intensity, the total leaves per plant harvested 4 times in all cultivation period was the great in order of $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 123, $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 107, $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 95, $50{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 56 which was the smallest number of total leaves harvest. Total fresh weight of above ground per plant was the high in order of $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 43.6g, $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 34.6g, $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 32.2g, $50{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 18.2g. From these results, it was concluded that photoperiod of 16/8 and light intensity at $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ can be used as the light condition of RBW LED (red:blue:white = 2:1:1) for optimal growth of Taraxacum coreanum Nakai in a fully artificial light type plant factory.

Comparison of flexural strength according to thickness between CAD/CAM denture base resins and conventional denture base resins (CAD/CAM 의치상 레진과 열중합 의치상 레진의 두께에 따른 굴곡 강도 비교)

  • Lee, Dong-Hyung;Lee, Joon-Seok
    • Journal of Dental Rehabilitation and Applied Science
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    • v.36 no.3
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    • pp.183-195
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    • 2020
  • Purpose: The purpose of this study is to compare the flexural strength of CAD/CAM denture base resins with conventional denture base resins based on their thicknesses. Materials and Methods: For the conventional denture base resins, Lucitone 199® (C-LC) was used. DIOnavi - Denture (P-DO) and DENTCA Denture Base II (P-DC) were taken for the 3D printing denture base resins. For the prepolymerized PMMA resins, Vipi Block Gum (M-VP) and M-IVoBase® CAD (M-IV) were used. The final dimensions of the specimens were 65.0 mm x 12.7 mm x 1.6 mm / 2.0 mm / 2.5 mm. The 3-point bend test was implemented to measure the flexural strength and flexural modulus. Microscopic evaluation of surface of fractured specimen was conducted by using a scanning electron microscope (SEM). After testing the normality of the data, one-way ANOVA was adopted to evaluate the differences among sample groups with a significance level of P = 0.05. The Tukey HSD test was performed for post hoc analysis. Results: Under the same thicknesses, there are significant differences in flexural strength between CAD/CAM denture base resins and conventional denture base resins except for P-DO and C-LC. M-VP showed higher flexural strength than conventional denture base resins, P-DC and M-IV displayed lower flexural strength than conventional denture base resins. Flexural modulus was highest in M-VP, followed by C-LC, P-DO, P-DC, M-IV, significant differences were found between all materials. In the comparison of flexural strength according to thickness, flexural strength of 2.5 mm was significantly higher than that of 1.6 mm in C-LC. Flexural strength of 2.5 mm and 2.0 mm was significantly higher than that of 1.6 mm in P-DC and M-VP. In M-IV, as the thickness increases, significant increase in flexural strength appeared. SEM analysis illustrates different fracture surfaces of the specimens. Conclusion: The flexural strength of different CAD/CAM denture base resins used in this study varied according to the composition and properties of each material. The flexural strength of CAD/CAM denture base resins was higher than the standard suggested by ISO 20795-1:2013 at a thickness of 1.6 mm or more though the thickness decreased. However, for clinical use of dentures with lower thickness, further researches should be done regarding other properties at lower thickness of denture base resins.

Gadolinium Complex of 1,4,7,10-Tetraazacyclododecane-1,4,7-trisacetic acid (DO3A) Conjugate of [(p-aniline benzothiazole)methyl]pyridine as a Tumor-Targeting MRI Contrast Agent

  • Nam, Ki Soo;Jung, Ki-Hye;Chang, Yongmin;Kim, Tae-Jeong
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3654-3658
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    • 2013
  • The synthesis of a DO3A conjugate of [(p-aniline benzothiazole)methyl]pyridine ($L^2H_3$) and its gadolinium complex of the type [$Gd(L^2)(H_2O)$] ($GdL^2$) is described. The $R_1$ relaxivity ($=4.50mM^{-1}sec^{-1}$) and kinetic inertness of $GdL^2$ compares well with those of structurally analogous Dotarem$^{(R)}$ ($R_1=3.70mM^{-1}sec^{-1}$), a typical extracellular (ECF) MRI contrast agent (CA). Yet, by comparison with Dotarem$^{(R)}$, $GdL^2$ exhibits non-covalent interactions with human serum albumin (HSA) as evidenced by the ${\varepsilon}^*$ titration curve along with in vivo MR signal enhancement in both aorta and heart. Liver-specific nature of $GdL^2$ is also observed as excretion is made through gallbladder. Most notably, $GdL^2$ further demonstrates specificity toward the MDA-MB-231 breast cancer.

Effects of C10- and C12-chain length alkyl analogs of monochamol on attraction of longhorn pine sawyer Monochamus saltuarius (Coleoptera: Cerambycidae)

  • LEE, Sung-Min;HONG, Do Kyung;JANG, Sei-Heon;LEE, Kyeong-Yeoll;LEE, ChangWoo
    • Entomological Research
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    • v.48 no.5
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    • pp.448-452
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    • 2018
  • The aggregation pheromone of Monochamus (Coleoptera: Cerambycidae) beetles, 2-(undecyloxy) ethanol (hereafter referred to as monochamol), has gained considerable attention because of its usefulness in monitoring and population control of pine sawyer beetles. The hydroxyether structural motif is conserved in pheromones of the subfamily Lamiinae of the Cerambycidae. In this study, we investigated the effects of C10- and C12-chain length alkyl analogs of monochamol, 2-(decyloxy) ethanol and 2-(dodecyloxy) ethanol, on attracting M. saltuairus in Andong, Gyeongsangbuk-do, Korea. The C10 and C12 analogs attracted M. saltuarius when used in combination with ${\alpha}$-pinene and ethanol, but the responses of these alkyl chain analogs were lower than those of monochamol. Furthermore, the addition of either C10 or C12 analog to the use of monochamol with ${\alpha}$-pinene and ethanol had no effect on attraction of M. saltuarius, indicating high sensitivity of M. saltuarius to monochanol. Taken together, the results of this study suggest that chemical communication within a Monochamus species depends not only on monochamol, but also on other semiochemicals.

Determination and confirmation of the carbendazim residue in soybean sprout (콩나물중 살균제 carbendazim 잔류분의 정량 및 확인)

  • Kim, Young-Gook;Park, Jong-Tae;Hong, Suk-Soon
    • The Korean Journal of Pesticide Science
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    • v.2 no.3
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    • pp.79-84
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    • 1998
  • Tandem HPLC and atmospheric pressure chemical ionization(APcI) LC/MS method was used for the determination and confirmation of carbendazim residues in soybean sprout. Fluorescence(FL) detector was connected in tandem with the ultraviolet(UV) detector for dual detection of the carbendazim residue at the excitation and emission wavelength of 280 nm and 310 nm, respectively. The limit of detection for carbendazim was $0.1{\mu}g/kg$ sample. Recoveries of carbendazim from fortified soybean sprout at 0.5, 1.0 and 2.0 ppm were averaged 89.1%. Mass spectrometry using a APcI source confirmed the carbendazim residue in the soybean sprout sample. Fragmentation pattern on the APcI LC/MS spectrum of carbendazim was simpler than that from electron impact(EI) mass spectrum. Carbendazim produced 3 major ions including m/z 133, m/z 159 and m/z 191($M^{+}$). This method was sensitive enough to provide reliable and reproducible results for practical applications.

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An Unrecorded Species of Pratylenchus Ioosi Loof (Tylenchida: Pratylenchidae) from Tea in Korea (한국산기종 차나무뿌리썩이선충, Pratylenchus Ioosi Loof(참선충 목: 뿌리썩이선충科))

  • Park, Byeong-Yong;Park, Dong-Ro;Lee, Jae-Kook;Park, Young-Eoun;Shin, Gil-Ho
    • Korean journal of applied entomology
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    • v.41 no.4
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    • pp.299-303
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    • 2002
  • Pratylenchus loosi Loof, 1960 is recorded for the first time from Korea. The nematode was isolated from the roots of tea (Thea sinensis L.) and soil around the roots from Yeongam-gun, Jeo-llanam-do and Namjeju-gun, Jeju-do, Korea. This species has two lip annuli. The female is 433-646 $\mu\textrm{m}$ long: a= 29.1-37.5, b = 5.1-6.4, c = 15.0-21.3, vulva(%) = 73.0-85.4. The male body length is 408-512$\mu\textrm{m}$ long: a=36.1-40.0, b=4.8-6.7, c =17.0-19.0, and spicule = 14.1-18.0 $\mu\textrm{m}$. The stylet is 11.6-18.0 $\mu\textrm{m}$ long and the number of incisures is four. The shape of spermatheca filled with sperm is broadly rounded, oval and quadrangular The shape of tail is narrowly round to subacute.

Improved cryptanalysis of lightweight RFID mutual authentication Protocols LMAP, $M^2AP$, EMAP (경량 RFID 상호인증 프로토콜 LMAP, $M^2AP$, EMAP에 대한 향상된 취약성 분석)

  • Kwon, Dae-Sung;Lee, Joo-Young;Koo, Bon-Wook
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.17 no.4
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    • pp.103-113
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    • 2007
  • In this paper, we present a security analysis of Lightweight RFID Mutual Authentication Protocols-LMAP[10], $M^2AP$[11], EMAP[12]. Based on simple logic operations, the protocols were designed to be suitable for lightweight environments such as RFID systems. In [8,9], it is shown that these protocols are vulnerable to do-synchronization attacks with a high probability. The authors also presented an active attack that partially reveals a tag's secret values including its ID. In this paper, we point out an error from [9] and show that their do-synchronization attack would always succeed. We also improve the active attack in [9] to show an adversary can compute a tag's ID as well as certain secret keys in a deterministic way. As for $M^2AP$ and EMAP, we show that eavesdropping $2{\sim}3$ consecutive sessions is sufficient to reveal a tag's essential secret values including its ID that allows for tracing, do-synchronization and/or subsequent impersonations.