• Title/Summary/Keyword: Dl protein

검색결과 339건 처리시간 0.029초

Transcriptional Regulation of the Methuselah Gene by Dorsal Protein in Drosophila melanogaster

  • Kim, Hyukmin;Kim, Jinsu;Lee, Yoonsoo;Yang, Jaeyeon;Han, Kyuhyung
    • Molecules and Cells
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    • 제21권2호
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    • pp.261-268
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    • 2006
  • The Drosophila methuselah (mth) mutant has an approximately 35 percent increase in average lifespan, and enhanced resistance to various forms of stress, including starvation, high temperature, and dietary paraquat. To examine the transcriptional regulation of mth, we used luciferase assays employing Drosophila S2 cells. Two positive control elements were found at -542 ~ -272 (PE1) and +28 ~ +217 (PE2), where putative binding sites for transcription factors including Dorsal (Dl) were identified. Cotransfection of a Dl expression plasmid with a mth-luciferase reporter plasmid resulted in decreased reporter activity. PE1 and PE2, the minimal elements for strong promoter activity, were required for maximal repression by Dl protein. The N-terminal Rel homology domain (RHD) of Dl was not sufficient for repression of mth. We demonstrated by chromatin affinity precipitation (ChAP) assays in S2 cells that Dl bound to the putative PE1 binding site. Unexpectedly, semi-quantitative RT-PCR analysis revealed that the level of mth transcripts was reduced in dl flies. However, the in vivo result support the view that mth expression is regulated by dl, since it is well known that Dl functions as both a transcriptional activator and repressor depending on what other transcription factors are present. These findings suggest that both innate immunity and resistance to stress are controlled by Dl protein.

Isolation and Characterization of an Eosinophilic GH 16 β-Agarase (AgaDL6) from an Agar-Degrading Marine Bacterium Flammeovirga sp. HQM9

  • Liu, Yan;Tian, Xiaoxu;Peng, Chao;Du, Zongjun
    • Journal of Microbiology and Biotechnology
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    • 제29권2호
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    • pp.235-243
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    • 2019
  • A special eosinophilic agarase exo-type ${\beta}$-agarase gene, AgaDL6, was cloned from a marine agar-degrading bacterium, Flammeovirga sp. HQM9. The gene comprised 1,383-bp nucleotides encoding a putative agarase AgaDL6 of 461 amino acids with a calculated molecular mass of 52.8 kDa. Sequence analysis revealed a ${\beta}$-agarase domain that belongs to the glycoside hydrolase family (GH) 16 and a carbohydrate-binding module (CBM_4_9) unique to agarases. AgaDL6 was heterologously expressed in Escherichia coli BL21 (DE3). Enzyme activity analysis of the purified protein showed that the optimal temperature and pH of AgaDL6 were $50^{\circ}C$ and 3.0, respectively. AgaDL6 showed thermal stability by retaining more than 98% of activity after incubation for 2 h at $50^{\circ}C$, a feature quite different from other agarases. AgaDL6 also exhibited outstanding acid stability, retaining 100% of activity after incubation for 24 h at pH 2.0 to 5.0, a property distinct from other agarases. This is the first agarase characterized to have such high acid stability. In addition, we observed no obvious stimulation or inhibition of AgaDL6 in the presence of various metal ions and denaturants. AgaDL6 is an exo-type ${\beta}$-1,4 agarase that cleaved agarose into neoagarotetraose and neoagarohexaose as the final products. These characteristics make AgaDL6 a potentially valuable enzyme in the cosmetic, food, and pharmaceutical industries.

중.노년층이 섭취한 식품 종류와 영양소섭취상태, 신체지수 및 혈중지질과의 관계 (The Study of Associations among Serum Lipids, Anthropometric Measurements, Food Intake Frequency and Nutrient Intake in Healthy Adults)

  • 이다홍;김인숙
    • 대한지역사회영양학회지
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    • 제5권4호
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    • pp.642-653
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    • 2000
  • The purpose of this study was to investigate the association among food intake frequency, nutrient intake, anthropometric measurements, and serum lipids in 100 women and 85 men aged 40-74yrs residing in Jeonju City. Food intake frequency and nutrient intake were obtained from one-day dietary recalls. The results were as follows : The mean height, body weight and BMI were 161 cm, 62.5 kg, and 24 kg/$m^2$respectively. The mean of SBP and DBP were 118 mmHg, 77 mmHg respectively. The mean of TC and TG were 185.5 mg/dl, 153 mg/dl respectively. The mean daily energy and carbohydrate intake was 85. 7%, 91% of RDA for Koreans respectively and that protein intake was 118.5% of RDA. The TC levels of subjects less than 75%, 75-125%, more than 125% of RDA energy were 176 mg/dl, 192 mg/dl, 185 mg/dl respectively(p < 0.01). The TC levels less than 75%, 75-125%, more than 125% of RDA protein were 180 mg/dl, 190 mg/dl, 175 mg/dl respectively(p < 0.05). TC levels less than 75%, 75-125%, more than 125% of RDA fat were 181 mg/dl, 193 mg/dl, 194 mg/dl respectively(p < 0.05). Of the subjects, 18% ate mushrooms. The BMI of the mushroom eaten was 24 and that of non-mushroom eaten was 25(p <0.05). Of the subjects, 28% consumed milk and milk produce. The mean level of TC and TG in milk and milk products eaters were 181 mg/dl, 131 mg/dl and that of non milk and milk products eaters were 197 mg/dl, 159 mg/dl(p < 0.001, p < 0.05). Of the subjects, 58% ate fish. The HDL-C of the fish eaten was 50 mg/dl and that of non-fishes eaten was 45 mg/dl(p < 0.05).

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수유부의 식품 및 영양소 섭취와 유즙 생성량 및 조성 (Composition and Yield of Korean Breast Milk and Maternal Intakes of Foods and Nutrients)

  • 이정아
    • Journal of Nutrition and Health
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    • 제27권8호
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    • pp.794-804
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    • 1994
  • This study was conducted to investigate the relationship between lactational capacity and intakes of energy and energy-yielding nutrients. Food consumption, intakes of carbohydrate, protein, fat and energy and quantity and proximate composition of milk of 11 lactating Korean mothers were determined at 1, 2 and 3 months postpartum longitudinally. Food consumption was estimated using a 24-hour recall method ; intakes of energy and energy-yielding nutrients were calculated according to the Food Composition Table. Daily milk production was estimated using a 72-hour test-weighing method ; protein, fat, lactose and energy concentrations were analyzed. Average intakes of energy and protein were 1974$\pm$386㎉/day and 67.0$\pm$12.3㎉/day, these were 73% and 74% of the Korean Recommended Allowances, respectively. Average milk yield was 720.1$\pm$123.3ml/day containing energy 59.6$\pm$9.5㎉/dl, protein 1.1$\pm$0.1g/dl, fat 2.7$\pm$0.8g/dl, and lactose 6.3$\pm$0.3g/dl. No relationship existed between the intakes of carbohydrate, protein, fat and energy and the quantity and proximate composition of the milk. However, the intakes of energy, carbohydrate and vegetable protein were inversely related to the concentrations of energy and lactose in the milk. This result indicates that lactational capacity may be affected by the other factors excluding intakes of energy and energy-yielding nutrients and a high intake of energy may not guarantee optimal lactational capacity.

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미립자 응집반응을 이용한 C-reactive Protein의 면역 측정 법에 관한 연구 (Microparticle-based Immunonassay for the Detection of C-reactive Protein in Serum)

  • 김재화;송은영;이희구;최용경;최명자;김용호;최인성;정태화
    • 대한의생명과학회지
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    • 제2권1호
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    • pp.41-48
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    • 1996
  • 환자의 복수와 늑막액으로부터 p-diazonium phenylphosphorylcholine(DPPC) coupled Separose-4B affinity chromatography와 hydroxylapatite chromatography를 실시하여 C-reactive protein (CRP)를 분리, 정제하였다. 정제된 CRP를 토끼에게 면역화하여 항혈청을 얻고 affinity chromatography를 하여 면역항체(IgG)를 분리하였다. 분리된 면역항체를 미립자에 감작시킨 후 미립자 응집반응에 의하여 3분내에 CRP를 측정할 수 있는 간이 면역 측정법을 개발하였다. 본 연구에서 개발된 CRP측정법의 검출범위는 0.5~20mg/㎗이며, 임상 시험 결과 0.7~2.9mg/㎗에서는 강한 응집 반응을, 5.O~l3.2mg/㎗에서는 약한 응집반응을 보였고 28mg/dl이상에서는 항원 과잉으로 인한(zone of Ag excess phenomenon) 위음성을 나타냈다. 74명의 환자 혈청을 대상으로 CRP의 농도를 조사한 결과 평균치는 3.8mg/dl이었으며 대부분의 환자에서는 10mg/dl 이하의 농도로 존재하였다. 그러므로 1차 판정시 음성을 나타낸 시료라도 혈청을 5~10배정도 희석하여 재분석한다면 오차없이 CRP 를 검출할 수 있었다. 환자 혈청을 검체로 하여 본 연구에서 개발한 면역측정법과 현재 수입 시판 중인 프랑스의 B사 제품과 일본의 I사 제품을 비교한 결과 좋은 상관관계를 보였다. 이와 같은 평가 분석을 통하여 볼 때 본 연구에서 개발한 간이 면역 측정법은 사용이 비교적 간편하며 신빙성이 있어 CRP를 스크리닝하는데 효과적임을 알 수 있었다.

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개 심장사상충 추출액 투여에 의한 Anaphylactoid Reaction (Anaphylactoid Reaction Induced by Administration Dirofilaria immitis Extracts)

  • 이지영;송근호;권현주;김명철;윤효인;김덕환
    • 대한수의학회지
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    • 제43권3호
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    • pp.507-516
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    • 2003
  • This study was performed to clarify the induction possibility of anaphylactoid reactions by the administration with the heartworm extracts, and, if any, to elucidate different virulences in terms of the protein concentrdtions and sexes of Dirofilaria immitis. Twenty three clinically healthy D. immitis-free adult dogs were used in the present study. The experimental animals were divided into 5 groups. Group A (5 heads) was administered with an female heartworm extract containing 0.1 g/dl protein concentration. Group B (4 heads) was administered with an male heartworm extract containing 0.1g/dl protein concentration. Group C (5 heads) was administered with an female heartworm extract containing 0.2 g/dl protein concentration. Group D (4 heads) was administered with an male heartworm extract containing 0.2 g/dl protein concentration. Group E (5 heads) was administered with an female heartworm extract containing 0.4 g/dl protein concentration. The changes of clinical symptoms and vital signs (body temperature, heart rate and respiration rate) were examined before and 5 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours and 24 hours after injection with the extracts, respectively. In addition, the changes of hematological values (RBC, PCV and total leukocytes counts), serum chemical values (ALP and CK) were determined. It was considered that heartworm extract could induce anaphylactoid reaction and adult female heartworm extract was more affective than those of adult male heartworm extract in the changes of clinical symptoms, vital signs, hematological values and serum chemical values.

충남지역 일부 여대생의 칼슘 섭취상태와 혈청 골대사지표 , 단백질 및 지질 수준과의 관계에 대한 연구 (Relation among Calcium Intake , Bone Metabolism Parameters , Serum Protein and Lipids of Female College Students in Chungnam)

  • 최미경;승정자;김미현
    • 대한영양사협회학술지
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    • 제6권2호
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    • pp.108-116
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    • 2000
  • The purpose of this study was to investigate the relationship among calcium intake, blood parameters related with bone metabolism, and serum lipids in healthy adults on self-selected diet. Subjects were consisted of 40 female college students residing in Chungnam. Anthropometric measurements, dietary intake measurements and blood collection were conducted. Serum concentrations of total protein, albumin, alkaline phosphates, leucine amino peptidase, BUN, calcium, inorganic phosphorus, and lipids were measured by biochemical analyzer and ICP spectrometer. The results were as follows. The mean age of subjects was 22.34 years and weight, height and BMI were 52.89kg, 161.29cm and 20.34, respectively. The daily mean energy and calcium intakes were 81.75% and 64.38% of RDA. The mean animal 1:2. The mean serum concentrations were 6.54g/dl(total protein), 4.12g/dl(albumin), 123.24U/(alkaline phosphates), 36.59U/l(leucine amino peptidase), 8.26mg/dl(calcium), 3.29mg/dl(inorganic phosphorus), 60.73mg/dl(triglyceride), 138.49mg/dl(total cholesterol), 65.95mg/dl(HDL-cholesterol), and 60.39mg/dl(LDL-cholesterol). There were no significant differences among calcium intake, bone metabolism parameters, and serum lipids when analyzed by Pearson's correlation coefficient. More systematic studies are required to investigate the roles of calcium in healthy persons on self-selected diets containing different levels of calcium.

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한우 및 돼지의 혈액화학치에 관한 연구 (Studies on the Blood Chemistry of Korean Native Cattle and Pigs)

  • 도재철;이창우;손재권;정종식
    • 한국동물위생학회지
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    • 제13권1호
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    • pp.49-53
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    • 1990
  • In order to know the range in blood chemical values and enzyme activities of korean native cattle and pigs being raised in kyongbuk province, the mean values of serum protein compositions, enzyme activities(GOT, GPT, ALP), cholesterol, glucose, bilirubin, creatinine, urea nitrogen and uric acid were determined and the results obtained were as follows ; 1. Mean contents of total protein, albumin, globulin and A / G ratio for the korean native male cattle were $7.28{\pm}0.86$ g / dl, 43.39{\pm}0.65$ g / dl, 43.83{\pm}0.92$ g / dl & 40.95{\pm}0.34$;for the male pigs were 47.39{\pm}0.84$ g / dl, 43.63{\pm}0.43$ g / dl, 43.77{\pm}0.88$ g / dl, 40.97{\pm}0.26$;for the female pigs were 47.60{\pm}0.68$ g/ dl, 43.54{\pm}0.58$ g / dl, 43.90{\pm}0.74$ g / dl, 41.02{\pm}0.34. 2.$ Mean activities of GOT, GPT and ALP for the korean native male cattle were 448.3{\pm}11.6$ U, 429.6{\pm}4.6$ U, 413.7{\pm}2.8$ U; for the male pigs were 458.6{\pm}10.6$ U, 438.6{\pm}5.3$ U, 424.9{\pm}9.2$ U; for the female pigs were 443.9{\pm}10.8$ U, 440.5{\pm}4.6$ U, 413.9{\pm}3.4$ U. 3. Mean contents of cholesterol, glucose and bilirubin for the korean native male cattle were 4108.7{\pm}31.8$ mg / dl, 488.2{\pm}26.4$ mg / dl, $0.69{\pm}0.52$ mg / dl; for the male pigs were 4105.9{\pm}18.3$ mg / dl, 4102.9{\pm}22.3$ mg / dl, 40.70{\pm}0.18$ mg / dl: for the female pigs were $113.1{\pm}20.9$ mg / dl , 4103.2{\pm}23.9$ mg / dl, 40.49{\pm}0.21$ mg / dl. 4. Mean contents of creatinine, urea nitrogen and uric acid for the korean native male cattle were 42.86{\pm}0.69$ mg / dl, $18.1{\pm}5.0$ mg / dl, 41.38{\pm}0.66$ mg /dl; for the male pigs were 42.20{\pm}0.51$ mg / dl, $18.2{\pm}4.3$ mg / dl, 40.29{\pm}0.10$ mg /dl: for the female pigs were 42.36{\pm}0.70$ mg / dl, $20.5{\pm}5.4$ mg / dl, 40.69{\pm}0.27$ mg / dl.

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Effect of Levels of Sodium DL-malate Supplementation on Ruminal Fermentation Efficiency of Concentrates Containing High Levels of Cassava Chip in Dairy Steers

  • Khampa, S.;Wanapat, Metha;Wachirapakorn, C.;Nontaso, N.;Wattiaux, M.A.;Rowlison, P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권3호
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    • pp.368-375
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    • 2006
  • Four rumen-fistulated dairy steers were randomly assigned according to a $4{\times}4$ Latin square design to investigate effects of supplementation levels of sodium dl-malate in concentrates on rumen ecology, ruminal fermentation, nitrogen balance, feed intake and digestibility of nutrients and ruminal microbial protein synthesis. The dietary treatments were cassava concentrate-based, containing sodium dl-malate supplementation at 0, 9, 18 and 27 g/hd/d with urea-treated rice straw (UTS) fed ad libitum. The experiment was conducted for four periods, each period lasting 21 days. Ruminal pH increased with incremental addition of malate (p<0.05). Additionally, molar proportions of propionate were higher in supplemented groups and was highest at 18 g/hd/d of malate supplement (p<0.05). Microbial protein synthesis tended to be higher in dairy steers receiving sodium dl-malate supplements and also was the highest at 18 g/hd/d. Variable bacterial populations, such as amylolytic, proteolytic and cellulolytic species were increased (p<0.05). Furthermore, protozoal populations were decreased significantly (p<0.05), while fungal zoospores were dramatically increased in dairy steers receiving sodium dl-malate supplement (p<0.05). These results suggested that supplementation of concentrate containing a high level of cassava chip at 18 g/hd/d with UTS in dairy steers could improve rumen fermentation efficiency and rumen microbial protein synthesis.

한국인 Fragile X 환자들의 혈청단백질 구성 (Composition of Serum Protein in Korean Fragile X Syndrome Patients)

  • 김종봉
    • 대한의생명과학회지
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    • 제2권1호
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    • pp.127-132
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    • 1996
  • 한국인 일반 정신박약자들의 핵형 및 혈청단백질을 분석한 결과는 다음과 같다. 정신박약자 35명 중 3명에서 fragile X 염색체를 발견하였고 그 빈도는 4~l5%였다. Fragile X 증후군 환자들의 혈청단백질의 농도는 5.73$\pm$0.89(g/ dl) 이었고 albumin과 globulin의 비는 0.86$\pm$0.14 이었다. 일반 정신박약자들의 혈청단백질 농도는 6.83$\pm$0.72(g/dl) 이었고 이중 albumin과 globulin의 비는0.87$\pm$0.47 이었다. Fragile X증후군환자 및 일반정신박약자들의 혈청단백질 농도 및 albumin과 globulin의 비는 정상인 및 Down 증후군환자들 보다 낮았다.

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