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Molecular Size Fractionation of Soil Fulvic Acid by Gel Filtration Chromatography and Analysis of Their Fluorescence Characteristics (겔 여과 크로마토그래피에 의한 토양 풀빅산의 분자량 분획 및 형광특성 분석)

  • Chung, Kun-Ho;Shin, Hyun-Sang;Lee, Wanno;Cho, Yeong-Hyun;Choi, Geun-Sik;Lee, Chang-Woo
    • Analytical Science and Technology
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    • v.17 no.2
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    • pp.163-172
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    • 2004
  • The molecular size distribution and fluorescence properties of soil fulvic acids (FA) were characterized by using gel filtration chromatography (GFC) and luminescence spectroscopy. The objectives of this work were to fractionate the FA extracted from a forest soil into different nominal molecular size using GFC system and to characterize the fluorescence properties (excitation, emission and synchronous) of these fractions using luminescence spectrometer. The GFC column was calibrated with polyethylene glycols, acetone and dextrane Blue. The total permeation volume of the GFC system was 404 mL and the void volume 130 mL. The GFC molecular weight of the soil FA was in the range of 190~8,900 Dalton and the molecular weight at the peak on the chromatogram was 930 Dalton. The fluorescence intensity ratio ($I_{498nm}/I_{390nm}$) was found to be increased with an increasing molecular weight. This results may suggest that the fulvic acid fractions with high molecular weight have large amount of the condensed aromatic compound.

The Effects of Melatonin on Cisplatin-Induced Renal Cortical Cell Injury in Rabbits

  • Kim, Chung-Hui;Han, Jin;Kim, Na-Ri;Park, Ju-Hee;Yang, Young-Churl;Kim, Eui-Yong
    • The Korean Journal of Physiology and Pharmacology
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    • v.5 no.3
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    • pp.223-230
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    • 2001
  • Melatonin, a pineal gland hormone, is believed to act as an antioxidant via the stimulation of radical detoxifying enzymes and scavenging of free radicals. In this study, effects of in vitro and in vivo treatments of melatonin on the cisplatin-induced lipid peroxidation, LDH release and plasma creatinine were determined in rabbit renal cortical cells. The level of malondialdehyde (MDA) was assayed as an index of lipid peroxidation and the level of LDH release as an indicator of cellular damage. In in vitro studies, cisplatin increased the levels of MDA and LDH release in a concentration-and time-dependent manner. Melatonin inhibited the cisplatin-induced lipid peroxidation and LDH release in a concentration-dependent manner. The minimal effective concentration of melatonin that significantly reduced the $300\;{\mu}M$ cisplatin-induced lipid peroxidation and LDH release was 1 mM. In in vivo studies, the levels of lipid peroxidation and LDH release in renal cortical cells increased significantly 24 or 48 hours after a single injection of cisplatin (6 mg/kg). When the cisplatin-injected rabbits were pretreated with 10 mg/kg of melatonin, a significant reduction in both lipid peroxidation and LDH release was observed. The plasma creatinine level increased from $0.87{\pm}0.07$ mg/dl in control to $6.33{\pm}0.54$ mg/dl in cisplatin-injected rabbits (P<0.05). Melatonin partially prevented the increase in serum creatinine level $(1.98{\pm}0.11\;mg/dl)$ by cisplatin (P<0.05). In the proximal tubules from cisplatin-treated group, tubular cells had microvilli of variable heights. Necrotic debris was seen in tubular lumens. In most of cells, the mitochondria and lysosomes were increased in frequency. The endocytic vacuoles were not prominent and distribution of the brush border was irregular and shortened. These cisplatin-induced morphological changes were moderate in the melatonin-pretreated group. These results suggest that the toxicity of cisplatin is associated with the generation of reactive oxygen free radicals and that melatonin is a powerful antioxidant, which prevents some of the adverse effects of cisplatin.

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Spatial reproducibility of complex fractionated atrial electrogram depending on the direction and configuration of bipolar electrodes: an in-silico modeling study

  • Song, Jun-Seop;Lee, Young-Seon;Hwang, Minki;Lee, Jung-Kee;Li, Changyong;Joung, Boyoung;Lee, Moon-Hyoung;Shim, Eun Bo;Pak, Hui-Nam
    • The Korean Journal of Physiology and Pharmacology
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    • v.20 no.5
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    • pp.507-514
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    • 2016
  • Although 3D-complex fractionated atrial electrogram (CFAE) mapping is useful in radiofrequency catheter ablation for persistent atrial fibrillation (AF), the directions and configuration of the bipolar electrodes may affect the electrogram. This study aimed to compare the spatial reproducibility of CFAE by changing the catheter orientations and electrode distance in an in -silico left atrium (LA). We conducted this study by importing the heart CT image of a patient with AF into a 3D-homogeneous human LA model. Electrogram morphology, CFAE-cycle lengths (CLs) were compared for 16 different orientations of a virtual bipolar conventional catheter (conv-cath: size 3.5 mm, inter-electrode distance 4.75 mm). Additionally, the spatial correlations of CFAE-CLs and the percentage of consistent sites with CFAE-CL<120 ms were analyzed. The results from the conv-cath were compared with that obtained using a mini catheter (mini-cath: size 1 mm, inter-electrode distance 2.5 mm). Depending on the catheter orientation, the electrogram morphology and CFAE-CLs varied (conv-cath: $11.5{\pm}0.7%$ variation, mini-cath: $7.1{\pm}1.2%$ variation), however the mini-cath produced less variation of CFAE-CL than conv-cath (p<0.001). There were moderate spatial correlations among CFAE-CL measured at 16 orientations (conv-cath: $r=0.3055{\pm}0.2194$ vs. mini-cath: $0.6074{\pm}0.0733$, p<0.001). Additionally, the ratio of consistent CFAE sites was higher for mini catheter than conventional one ($38.3{\pm}4.6%$ vs. $22.3{\pm}1.4%$, p<0.05). Electrograms and CFAE distribution are affected by catheter orientation and electrode configuration in the in-silico LA model. However, there was moderate spatial consistency of CFAE areas, and narrowly spaced bipolar catheters were less influenced by catheter direction than conventional catheters.

An Approximate Estimation of Snow Weight Using KMA Weather Station Data and Snow Density Formulae (기상청 관측 자료와 눈 밀도 공식을 이용한 적설하중의 근사 추정)

  • Jo, Ji-yeong;Lee, Seung-Jae;Choi, Won
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.2
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    • pp.92-101
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    • 2020
  • To prevent and mitigate damage to farms due to heavy snowfall, snow weight information should be provided in addition to snow depth. This study reviews four formulae regarding snow density and weight used in extant studies and applies them in Suwon area to estimate snow weight in Korea. We investigated the observed snow depth of 94 meteorological stations and automatic weather stations (AWS) data over the past 30 years (1988-2017). Based on the spatial distribution of snow depth by area in Korea, much of the fresh snow cover, due to heavy snowfall, occurred in Jeollabuk-do and Gangwon-do. Record snowfalls occurred in Gyeongsangbuk-do and Gangwon-do. However, the most recent heavy snowfall in winter occurred in Gyeonggi-do, Gyeongsangbuk-do, and Jeollanam-do. This implies that even if the snow depth is high, there is no significant damage unless the snow weight is high. The estimation of snow weight in Suwon area yielded different results based on the calculation method of snow density. In general, high snow depth is associated with heavy snow weight. However, maximum snow weight and maximum snow depth do not necessarily occur on the same day. The result of this study can be utilized to estimate the snow weight at other locations in Korea and to carry out snow weight prediction based on a numerical model. Snow weight information is expected to aid in establishing standards for greenhouse design and to reduce the economic losses incurred by farms.

Relationship Between Academic Stress and Hopelessness Among Female High School Students (일부 여자고등학생들의 학업스트레스와 절망감과의 관련성)

  • Cho, Eun-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.1
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    • pp.593-603
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    • 2020
  • This study identified the relationship between academic stress and hopelessness among female high school students. The self-administered questionnaires were given to 334 female high school students located in D-city during the period from August 20, 2018 to September 20, 2018. As a result, the distribution of the level of hopelessness among those surveyed was 61.6% for the normal range group, 23.1% for the mild range group, 12.9% for the moderate range group, and 2.4% for the severe range group. The level of hopelessness, according to the academic stress of the study subjects, was significantly higher in the higher academic stress group than that in the lower academic stress group (p=0.000). Hopelessness also showed significant positive correlation with academic stress (r=0.371, p<0.01). The results of the logistic regression analysis showed that the risk ratio of hopelessness was significantly increased 9.27 times in the higher academic stress group when compared to that in the lower academic stress group. According to the multiple regression analysis, the significant variables affecting hopelessness were satisfaction with school life, conversation with parents, eating regularly, stress due to tests, stress due to studies and stress about career paths. Given the above results, the hopelessness of female high school students is significantly related to academic stress. Therefore, it is necessary to develop and implement programs that can properly control academic stress factors.

Study of the Changes in Knowledge Industrial Center Layout Planning Types and Outside Space Composition Elements -Focusing on Guro-gu and Geumcheon-gu in semi-industrial district in Seoul- (지식산업센터 배치유형과 외부공간구성 요소 변화에 관한 연구 -서울 구로구와 금천구 준공업지역을 중심으로-)

  • Lee, Sang-Kyoon;Oh, Joon-Gul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.10
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    • pp.6344-6353
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    • 2014
  • According to the changes in society, the knowledge industrial center, which is a factory related to the manufacturing industry, is now including various programs, such as information and communication knowledge. This study defined the knowledge industrial center related law into three periods: deduct layout planning type and outside space composition elements by previous research; case analysis; and suggestions of the direction of layout planning and outside space plan. As a result of the analysis, first, before 1999, the layout of the building is made mainly with a car, but depending on the form of the site, it is configured as a centered, side layout. Second, in the case of distribution, the support facility on the lower part is integrated and the factory on the higher part is distributed, so it is partly integrated and the building is unified. Third, beginning with the activation of privately owned public space in a semi-industrial district according to architecture regulations in 1999, the outside space is wider but the construction area is reduced, so the outside space composition has been changing in a similar manner to the planning methods of business facilities, such as office buildings.

Distribution of potential risky species on phytoplankton at ports in Korea (항만 환경에서 식물플랑크톤 잠재적 위해종의 분포)

  • Kwon, Oh Youn;Kang, Jung-Hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.506-510
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    • 2013
  • This study aimed to understand relationship between potential risky species and environmental factors at ports in Korea. During the study periods, 25 potential risky species (red tide and toxic species) representing 20 red-tide species, 5 toxic species were observed in the all ports. Skeletonema costatum (red-tide species) was predominated in all study area. This species showed positive correlation with pH, while negative correlation with dissolved oxygen (p<0.05) at Busan port. Also, this species showed positive correlation with total suspended solids and pH (p<0.05) at Ulsan port. However, Sk. costatum showed positive correlation with nitrate at Incheon port (p<0.01). Pseudo-nitzschia spp. producing amnesic shellfish poison (domoic acid) showed positive correlation with nitrate and silicate in all study areas (p<0.05). Alexandrium spp. (paralytic shellfish poison) and Dinophysis acuminata (diarrhetic shellfish poison) were affected by chemical oxygen demand (p<0.01). Our results indicated that red-tide species were affected by physical factors, while chemical factors affected toxic species.

A study on the hydration of sludge from limestone washing process in a steel making factory (제철소 석회석수세슬러지의 수화반응 특성에 관한 연구)

  • Ahn, Ji-Whan;Kim, Ka-Yeoun;Kim, Hwan
    • Resources Recycling
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    • v.3 no.3
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    • pp.32-49
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    • 1994
  • Hydration process is one of the basic carbonation system. Limestone sludge produced in Pohang Iron & Steel Co., Ltd. We tested for identify of hydration characteristics. The result obtained in this study can be summarized as follows; 1. The classify of limestone sludge is type of ground calcium carbonate(-3mm+325mesh)and the major mineral of calcite, and further more high grade(CaO 51%), fine powder(15~22$\mu\textrm{m}$). 2. Limestone sludge mixed two process sludge, first one is washing process sludge and the other one is wet collect kiln dust. The composition rate is about 8:2. Wet collect kiln dust is major mineral of calcite, too. But the sludge is assumed to one by quick lime, slaked lime and unreacted natural limestone. So, the ideal process is dividing of the washing process sludge and wet collect kiln dust. 3. We manufactured of slaked lime from limestone sludge. To investigate the effect of hydration reactor, the experiments was done with various reactor type as magnetic stirrer, shaking incubator and ultrasonic vibration reactor, respectively. Generally, ultrasonic vibration reactor is excellent hydration for limestone sludge and produced very fine slaked lime powder with ideal distribution. 4. The optimum condition is 10% pulp density, when the manufacture of fine slaked lime powder by ultrasonic vibration reactor. And hydration times to compare the results of the study with ultrasonic vibration reactor of generalized most short time(5~10 min). 5. Finally, the dispersive characteristics of slaked lime powder measured 1~5 $\mu\textrm{m}$ from limestone sludge were compared with those of natural limestone ones(10~20$\mu\textrm{m}$), in order to check applicability of slaked lime with hydration process from limestone sludge.

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Reconstruction of the Volcanic Lake in Hanon Volcano Using the Spatial Statistical Techniques (공간통계기법을 이용한 하논화산의 화구호 복원)

  • Choi Kwang-Hee;Yoon Kwang-Sung;Kim Jong-Wook
    • Journal of the Korean Geographical Society
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    • v.41 no.4 s.115
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    • pp.391-403
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    • 2006
  • The Hanon volcano located in the southern pan of Cheju Island, Korea has a wetland in its crater being used as a farmland. Previous researchers presumed this wetland was a maar lake in the past. Based on the seismic refraction method, the wetland sediment layer was estimated between 5 to 14 m deep, which is mostly in accordance with previous researches. However, this shows only the depths at some sites, not representing the whole spatial distribution. This study is an attempt to reconstruct the volcanic lake in Hanon crater by applying the spatial statistical techniques based on the depth information from the seismic survey and known data. The procedure of reconstruction is as follows: First, the depth information from the seismic survey and known data were collected and it was interpolated by IDW and Ordinary Kriging method. Next, with the interpolation map and the present DEM the paleo DEM was constructed. Finally, using the paleo lake level on core data, the boundary of volcanic lake was extracted from the paleo DEM. The reconstructed lake resembles a half-moon in the north of the central scoria cone. It is estimated that the lake was 5 m deep on average and 13 m deep at the deepest point. Although there are slight differences according to the interpolation techniques, it is calculated that the area of the lake was between 184,000 and $190000m^2,$ and its volume approximately $869,760m^3$. Because of the continuous deposition processes after the crater formation, the reconstructed volcanic lake would not indicate an actual lake at a specific time. Nevertheless, it offers a significant clue regarding the inner morphology and evolution of the crater.

Properties of Deposits and Geomorphic Formative Ages on Marine Terraces in Gwangyang Bay, South Sea of Korea (광양만 일대 해안단구의 퇴적물 특성과 지형 형성시기)

  • Lee Gwang-Ryul;Park Chung-Sun
    • Journal of the Korean Geographical Society
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    • v.41 no.3 s.114
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    • pp.346-360
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    • 2006
  • In order to analyze synthetically geomorphological processes of marine terrace in Korea, this study deals with the distribution of marine terraces, stratification of sedimentary layers, physicochemical properties of deposits, and formative ages of marine terraces based on OSL(Optically Stimulated Luminescence) absolute age at coastal area of Gwangyang Bay in central part of the South Coast. As a result of comparison with physicochemical properties on diverse geomorphic materials, there is not enough distinction in them, because of recycling and mixing of materials at Gwangyang Bay having a geomorphic closure. In Gwangyang bay coast, marine terraces are discovered at least 3 levels and have a small area. Formative age of 1st Terrace, as the lowest level ranging in $10{\sim}13m$ above the sea level, is estimated at MIS(Marine Isotope Oxygen Stage) 5a, based on OSL age dating and properties of deposits. Uplifting rate is calculated at 0.141m/ka in Gwangyang bay coast. For application to this rate, 2nd terrace($18{\sim}22m$) is estimated at MIS 5e, 3rd terrace($27{\sim}32m$) is latter part of MIS 7. Consequently, we might conclude that uplifting and geomorphic process of marine terrace in South Coast is similar to East Coast during the Late Pleistocene in Korea.