• 대한수의학회지
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• 제49권3호
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• pp.221-229
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• 2009

Foot-and-mouth disease (FMD) is the most contagious disease of mammals. The use of inactivated vaccine can be chosen to prevent or control FMD. However, vaccination against one serotype of FMDV doses not cross-protect against other serotypes and may not protect fully against some strains of the same serotype. Appropriate selection of vaccine strain is an important element in the control of FMD. The immunity of vaccine antigens should be matched against newly circulating viruses. The phylogenetic analysis of serotype Asia1 reported from China, Mongolia, North Korea and Russia since 2005 shows that they are all classified into genetic group V, but the strain, Asia1/Shamir (ISR/89) which have been used as a vaccine strain in Korea, is clustered into different genetic group. So, in this study the serological relationship between the isolate (Asia1/MOG/05; MOG) and the Shamir strain was determined by ELISA and virus neutralization test. Even though the matching value of the virus (MOG) against the vaccinated sera in target animals was not so high, the vaccinated animals elicited antibodies enough for protection after vaccinated once or twice. Conclusively, we suggest that the vaccine containing Asia1/Shamir antigen could protect the genetic group V strains circulating in East Asia currently if vaccinated twice or the more.

• Molecules and Cells
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• 제45권10호
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• pp.702-717
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• 2022

Hepatitis C virus (HCV) infection can lead to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. HCV employs diverse strategies to evade host antiviral innate immune responses to mediate a persistent infection. In the present study, we show that nonstructural protein 5A (NS5A) interacts with an NF-κB inhibitor immunomodulatory kinase, IKKε, and subsequently downregulates beta interferon (IFN-β) promoter activity. We further demonstrate that NS5A inhibits DDX3-mediated IKKε and interferon regulatory factor 3 (IRF3) phosphorylation. We also note that hyperphosphorylation of NS5A mediates protein interplay between NS5A and IKKε, thereby contributing to NS5A mediated modulation of IFN-β signaling. Lastly, NS5A inhibits IKKε-dependent p65 phosphorylation and NF-κB activation. Based on these findings, we propose NS5A as a novel regulator of IFN signaling events, specifically by inhibiting IKKε downstream signaling cascades through its interaction with IKKε. Taken together, these data suggest an additional mechanistic means by which HCV modulates host antiviral innate immune responses to promote persistent viral infection.

• Journal of Microbiology and Biotechnology
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• 제25권3호
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• pp.317-320
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• 2015

H3N8 equine influenza virus (EIV) causes respiratory diseases in the horse population, and it has been demonstrated that EIV can transmit into dogs owing to its availability on receptors of canine respiratory epithelial cells. Recently, we isolated H3N8 EIV from an EIV-vaccinated horse that showed symptoms of respiratory disease, and which has a partially truncated nonstructural gene (NS). However, it is not clear that the NS-truncated EIV has an ability to cross the host species barrier from horses to dogs as well. Here, we experimentally infected the NS-truncated H3N8 EIV into dogs, and monitored their clinical signs and viral load in respiratory organs to determine the virus's transmissibility.

• BMB Reports
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• 제53권7호
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• pp.373-378
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• 2020

Phosphorylation of the signaling component by protein kinase often leads to a kinase cascade or feedback loop. 3-Phosphoinositide-dependent kinase 1 (PDK1) signaling pathway diverges into various kinases including Akt and p70 S6 kinase (p70S6k). However, the PDK1 feedback mechanism remains elusive. Here, we demonstrated that UNC-51-like kinase (ULK1), an autophagy initiator kinase downstream of mechanistic target of rapamycin (mTOR), directly phosphorylated PDK1 on serine 389 at the linker region. Furthermore, our data showed that this phosphorylation affected the kinase activity of PDK1 toward downstream substrates. These results suggest a possible negative feedback loop between PDK1 and ULK1.

• Journal of Microbiology and Biotechnology
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• 제28권6호
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• pp.1030-1036
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• 2018

Bacillus strains produce various types of antibiotics, and random mutagenesis has traditionally been used to overproduce these natural metabolites. However, this method leads to the accumulation of unwanted mutations in the genome. Here, we rationally designed a single nucleotide substitution in the degU gene to generate a B. subtilis strain displaying increased plipastatin production in a foreign DNA-free manner. The mutant strain (BS1028u) showed improved antifungal activity against Pythium ultimum. Notably, pps operon deletion in BS1028u resulted in complete loss of antifungal activity, suggesting that the antifungal activity strongly depends on the expression of the pps operon. Quantitative real-time PCR and lacZ assays showed that the point mutation resulted in 2-fold increased pps operon expression, which caused the increase in antifungal activity. Likewise, commercial Bacillus strains can be improved to display higher antifungal activity by rationally designed simple modifications of their genome, rendering them more efficient biocontrol agents.

• Molecules and Cells
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• 제42권10호
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• pp.729-738
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• 2019

Autophagy is an important process for protein recycling. Oligomerization of p62/SQSTM1 is an essential step in this process and is achieved in two steps. Phox and Bem1p (PB1) domains can oligomerize through both basic and acidic surfaces in each molecule. The ZZ-type zinc finger (ZZ) domain binds to target proteins and promotes higher-oligomerization of p62. This mechanism is an important step in routing target proteins to the autophagosome. Here, we determined the crystal structure of the PB1 homo-dimer and modeled the p62 PB1 oligomers. These oligomer models were represented by a cylindrical helix and were compared with the previously determined electron microscopic map of a PB1 oligomer. To accurately compare, we mathematically calculated the lead length and radius of the helical oligomers. Our PB1 oligomer model fits the electron microscopy map and is both bendable and stretchable as a flexible helical filament.

• 한국데이터정보과학회:학술대회논문집
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• 한국데이터정보과학회 2001년도 추계학술대회
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• pp.76-78
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• 2001

Variable selection in oriental medical research is considered. Decision tree analysis algorithms such as CHAID, CART, C4.5 and QUEST have been successfully applied to a medical research. Paralysis disease is a highly dangerous and murderous disease which accompanied with a great deal of severe physical handicap. In this paper, we explore the use of CHAID algorithm for selecting variables for evidence-diagnosis of paralysis, disease. Empirical results comparing our proposed method to the method using Wilks $\lambda$ given.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2015년도 춘계학술대회 논문집
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• pp.66-66
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• 2015

• 식물병연구
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• 제26권1호
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• pp.19-28
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• 2020

대추나무 빗자루병은 1950년대에 남한지역에 대발생하여 대추 재배지를 황폐화시킨 고위험 식물병이다. 최근 전국적으로 대추 재배면적이 크게 증가하고 있어 빗자루병 대발생 위험성을 사전에 파악하기 위한 기초자료를 얻고자 2017년과 2019년에 걸쳐 보은 지역의 대추 과원에서 병조사를 하였다. 또한, 빗자루병 조기 진단에 활용할 수 있는 다양한 병징을 찾아내고, 성보싸이클린수화제(OTC, 옥시테트라사이클린칼슘알킬트리메틸암모늄 수화제, 유효성분 17%)의 수간주사 방제효과 증진을 위한 연구를 수행하였다. 그 결과 현재까지 충북 보은 지역의 대추나무 빗자루병 발병률(이병주율)은 매우 낮았으나, 발생 지역과 발생 농가를 중심으로 점차 의미 있게 증가하고 있었다. OTC 방제시험 결과 처리 시기는 대추나무 생육 초기가 가장 효과적이었다. 그러나 발병이 심한 나무에서는 수간주사 후 당해 연도에도 같은 나무의 다른 건전한 가지에서 병징이 발현되었다. 6월에 1차 수간주사를 한 처리에서 2차 수간주사는 5개월 후보다 한 달 후에 하였을 때 방제효과가 더 좋았다. 이러한 결과는 조기 병징을 활용한 진단과 이를 근거로 한 조기 방제가 빗자루병 피해를 사전에 차단하고 병 방제효과를 높이는 데 매우 효과적일 수 있음을 시사한다.

• Journal of Acupuncture Research
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• 제26권4호
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• pp.91-98
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• 2009

Objectives : The objective of this review is to introduce the basic concepts on the febrile disease in the Mongolian Traditional Medicine(MTM). Methods : We comprehensively reviewed related textbooks in Mongolian and articles searched from China National Knowledge Infrastructure(CNKI) on febrile disease about MTM and summarized the pathophysiologic basis of, and treatment principles in MTM. Results and Conclusions : 1. In MTM, there are basic concepts such as theory of Arga-Bilig, theory of five elements, theory of three elements(人體三素), theory of seven constitutions and three discharges(七元三濊). Most of all, theory of three elements, which is about the basic elements of human body in MTM, is important fundamental notions for explaining human physiology and pathology. In MTM, It is explained that the imbalance of three elements, namely khii, shar, bad-kan is the root of all disease. Especially, febrile disease is treated by suppressing activated shar and blood(血液), which have fire characters, and managing various additional symptoms by balancing three elements and eliminating miasma(邪氣), inappropriate substances such as bile water(shar yc, 黃水) or blood stasis(惡血) simultaneously. 2. In MTM, both of internal treatment modalities(various medicinal therapy) and external treatment modalities(physical therapy) have been used for febrile disease. Food therapy and life style modification also have been recognized as important treatment methods. These aspects correspond to wholism theory of Korean Traditional Medicine(KTM). Further studies about details of each treatment methods will discover new applicable treatment method and make broaden the external boundaries of the KTM.