• Molecules and Cells
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• v.44 no.1
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• pp.26-37
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• 2021

Human papillomaviruses (HPVs) cause cellular hyperproliferation-associated abnormalities including cervical cancer. The HPV genome encodes two major viral oncoproteins, E6 and E7, which recruit various host proteins by direct interaction for proteasomal degradation. Recently, we reported the structure of HPV18 E7 conserved region 3 (CR3) bound to the protein tyrosine phosphatase (PTP) domain of PTPN14, a well-defined tumor suppressor, and found that this intermolecular interaction plays a key role in E7-driven transformation and tumorigenesis. In this study, we carried out a molecular analysis of the interaction between CR3 of HPV18 E7 and the PTP domain of PTPN21, a PTP protein that shares high sequence homology with PTPN14 but is putatively oncogenic rather than tumor-suppressive. Through the combined use of biochemical tools, we verified that HPV18 E7 and PTPN21 form a 2:2 complex, with a dissociation constant of 5 nM and a nearly identical binding manner with the HPV18 E7 and PTPN14 complex. Nevertheless, despite the structural similarities, the biological consequences of the E7 interaction were found to differ between the two PTP proteins. Unlike PTPN14, PTPN21 did not appear to be subjected to proteasomal degradation in HPV18-positive HeLa cervical cancer cells. Moreover, knockdown of PTPN21 led to retardation of the migration/invasion of HeLa cells and HPV18 E7-expressing HaCaT keratinocytes, which reflects its protumor activity. In conclusion, the associations of the viral oncoprotein E7 with PTPN14 and PTPN21 are similar at the molecular level but play different physiological roles.

• (The) Korean Journal of Educational Psychology
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• v.26 no.4
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• pp.1225-1248
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• 2012

The present study explored how ADHD assessment, identification, and diagnosis were conducted in research through an overview of research papers on ADHD in the last 10 years. The collected studies were categorized and analyzed by topics including data collection settings, assessment and identification, coexistence with other disabilities, considerable variables throughout evaluation, and biological basis. They were categorized and analyzed according to the identification methods, the number of assessment(s) and the number of informant(s). The results were as follows: First, the assessment and identifying studies on children with ADHD were excessively biased toward clinical settings. Second, the majority of the previous studies explored more the development or validation of tools for the assessment and identifying of ADHD than the other topics. Finally, a high preference for the use of a multiple assessment method in identifying ADHD was found. However, considering methodological aspects, there was a lack of practical application of multi-step process such as including a personal interview, reviewing school records or direct observing rating criteria. Considering these results, we discussed an urgent need for the diagnosis and identification guidelines of ADHD for teachers to effectively identifying ADHD children at schools. The implications of school-based ADHD assessment and diagnosis research were suggested.

• The Journal of the Korea Contents Association
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• v.21 no.6
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• pp.404-414
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• 2021

This study was to investigate the differences of functional health status and medical service experience and needs between general and vulnerable older people in community. This study is a cross-sectional descriptive research. The data obtained through direct visit surveys from November to December 2016. The target population of the study was older people over 65 years old, the final study subjects were 444 older people residing in one district of Seoul. The chi-square test was conducted to confirm the difference in their functional health areas and medical service experiences, and the necessity of medical service utilization in accordance to the social class. In the experience of abnormality in functional health, the vulnerable older people had higher experience in cognitive function, nutrition, hydration, pain, and falling than the general older people. The rate of experience of using medical service to solve the cognitive function problem for general older people was 31.9%, higher than that of the vulnerable older people. In contrast, the medical service utilization needs of the vulnerable older people in the pain management category was significantly higher than that of the general older people. In setting policy of public medical service programs for general and vulnerable older people in community may be differentially developed based on this study. In order to improve the medical accessibility of the vulnerable older people, public medical institutions should be actively supported to overcome obstacles to medical use due to economic barriers.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.5
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• pp.95-102
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• 2020

For the purpose of developing a PE fiber-reinforced highly ductile cementitious composite having high tensile strain capacity more than 2% under the condition of containing aggregates with large particle size, this study investigated the tensile behavior of composites according to the particle size and distribution of aggregates in the composite. Compared with the mixture containing silica sand of which particle size is less than 0.6 mm, mixtures containing river sand and/or gravel with the maximum particle size of 2.36 mm, 4.75 mm, 5.6 mm, 6.7 mm were considered in the experimental design. The particle size distributions of aggregates were adjusted for the optimized distribution curves obtained from modified A&A model by blending different sizes of aggregates. All the mixtures presented clear strain-hardening behavior in the direct tensile tests. The mixtures with the blended aggregates to meet the optimum curves of aggregate size distributions showed higher tensile strain capacity than the mixture with silica sand. It was also found that the tensile strain capacity was improved as the maximum size of aggregate increased which resulted in wider particle size distribution. The mixtures with the maximum size of 5.6 mm and 6.7 mm presented very high tensile strain capacities of 4.83% and 5.89%, respectively. This study demonstrated that it was possible to use coarse aggregates in manufacturing highly ductile fiber-reinforced cementitous composite by adjusting the particle size distribution.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.120-130
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• 2020

This study examined the effects of perceived value (functional value, social value) and stress (complexity, insecurity) on continuous intention in SNS. In particular, this study examined the direct and indirect impacts of SNS type on the intention of continuing to use SNS. The research model was tested for 461 respondents that used SNS. SPSS 21.0 and PLS 2.0 techniques were used to perform reliability, feasibility, path and hierarchical regression analysis. The summary of the research results is as follows. First, the results indicate that functional value and social value have positive effects on satisfaction. Second, the results indicate that complexity had a negative effect on satisfaction. Third, the results indicate that insecurity and satisfaction have positive effects on continuous intention. Fourth, the results indicate that SNS type had a negative effect on continuous intention. Fifth, it was shown that SNS type had a moderating effect in the relationship between insecurity and continuous intention. Specifically, Insecurity had a stronger effect on continuous intention at closed-type SNS than open-type SNS. These findings suggest that closed-type SNS companies will have to address the anxiety of SNS users by providing various items and system devices.

• Journal of Dental Rehabilitation and Applied Science
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• v.37 no.4
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• pp.232-243
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• 2021

Purpose: The purpose of this study was to evaluate the shear bond strength of various 3D printed denture base resins and the conventional denture base resin to various denture relining materials. Materials and Methods: For denture base materials, a heatcured (Vertex RS) and two types of 3D printed DENTCA Denture base II, NextDent^TM Base) were used. And 4 types denture relining materials (Tokuyama Rebase II fast, Kooliner, Denture Liner, Denture Liner, Lang Jet Denture Repair Kit) with different components were used. It was classified into 12 groups. Adhesion was performed between the resin base and the relining materials in accordance with ISO/TS 11405 standard. The shear bonding strength was measured, and then the adhesion interface was observed with a stereoscopic microscope and a scanning electron microscope. The fracture pattern was investigated through the analysis of the fragment. Results: In the 3D printed denture resin group, the shear bonding strength with relining materials was significantly lower than that of the heat-cured resin group (P < 0.05). The group of polymethyl methacrylate -based relining materials, high shear bonding strength was shown regardless of the type of denture. As for the fracture pattern, adhesive fracture appeared in most groups, and cohesive, mixed fracture appeared in some groups. Conclusion: The polymethyl methacrylate -based denture relining materials showed high shear bonding strength values compared to other denture relining materials. But, for direct methods, it is considered advantageous in terms of shear bonding strength to use a isobutyl methacrylate-based denture relining materials.

• The Journal of the Korea Contents Association
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• v.22 no.6
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• pp.629-637
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• 2022

It is at risk of depletion of biodiversity due to indiscriminate overfishing of ecosystems and destruction of habitats. Intensive fertilizers or development of related facilities to increase agricultural production in poor indigenous areas devastate the soil. Preservation of biodiversity is now emerging as an important issue of global human coexistence. After the Post-2020 GBF Declaration, all governance in agricultural development in indigenous agricultural areas should be supported and promoted as biodiversity conservation measures. A compromise plan to reduce ecosystem development and biodiversity loss can help establish public governance policies. In this paper, a viability kernel used for viable control feedback analysis is introduced to solve conflicting economic and ecological problems in ecosystem conservation, and a mathematical model on biodiversity conservation by the viability kernel is examined. Because all species in the ecosystem are interdependent, if the balance is broken, biodiversity is depleted, which is irreversible and eventually leads to extinction. For sustainable use and harmony of biological resources, a lot of policy consideration is required, such as creative governance that can efficiently protect all species. Subsidies or tax incentives have a direct impact on biodiversity conservation. The recovery of species in a state of decreasing biodiversity can be said to be of great economic value. Biodiversity will allow indigenous producers to be proud of their unique traditional knowledge and have a positive impact on local tourism, thereby enhancing regional identity and greatly contributing to the survival and prosperity of mankind.

• Korean Journal of Psychosomatic Medicine
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• v.30 no.1
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• pp.7-15
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• 2022

Objectives : The purpose of this study is to identify the effect of office workers' stress level and stress vulnerability on heart rate variability (HRV), confirm the significance of objective stress indicators obtained through subjective stress and heart rate variability devices, and examine their effectiveness as an integrated stress measurement tool in community mental health projects. Methods : From June to July in 2020, 929 workers participated in the stress management and mental health promotion project carried out by K hospital, and their recorded database was used for study analysis with their agreements. Multiple linear regression analysis was performed to find out the effects of general stress level, and stress vulnerability on heart rate variability. Results : All general characteristics were found to have a significant effect on SDNN (ln), RMSSD (ln), LF (ln), and HF (ln), but work experience did not significantly affect RMSSD (ln). Stress level and stress vulnerability did not significantly affect heart rate variability, but stress perception, a sub-factor of stress level, was found to have a significant effect on RMSSD (ln) (β=0.118, p=0.023). Conclusions : In this study, stress perception, a sub-factor of stress level, was found to be a factor affecting RMSSD (ln). This indicates an association with the activity of parasympathetic nerves in stressful situations, and more follow-up studies are needed to use it as a direct indicator of chronic stress and integrated stress in the community mental health field.

• Journal of Korea Entertainment Industry Association
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• v.15 no.4
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• pp.325-335
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• 2021

Chronic diseases have been increasing recently as the average life expectancy of humans has been extended, and this trend has caused problems such as the widespread demand for health and rehabilitation services and rising medical costs. In order to solve this problem, the community-based rehabilitation has been developed and strengthened in Korea and gradually promoted since 2019. It is important to secure access to clients who want to use services to revitalize community-based rehabilitation. So in this study, as part of the community-based rehabilitation, intends to devise smartphone applications designs and develop a prototype to secure access to community-based occupational therapy services based on occupation and activities. For Occupational Therapy Practice Framework (OTPF), International Classification of Functioning, Disability and Health (ICF), and Allen Diagnostic Module-2 (ADM-2) were used to devise and categorize occupation and activity based application content, and link OTPF, ICF, and ADM-2 through prior research analysis and expert meetings. The derived content was visualized through literature review and activity analysis, and was implemented to enable direct playback within the application using the YouTube API, and finally developed a prototype application. The Android Studio 3.5.2 for Windows 64-bit was used to build the application prototype. In further research, converging various digital technologies for user convenience and additionally researching community-based occupational therapy service providers opinions and service user satisfaction will improve accessibility to community-based occupational therapy services for clients who have difficulty occupational performance in the community.

• Korean Journal of School Psychology
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• v.17 no.2
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• pp.181-198
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• 2020

This study examined how the rejection sensitivity model might be applied to the link between rejection sensitivity and dating relationship satisfaction by means of investigating the mediating effect of cognitive emotion regulation strategy and conflict resolution strategy. A total of 668 college students in Seoul (232 males, 436 females, mean age of 21.59 years [SD=2.08]) participated in this study. The results illustrated that the adaptive emotion regulation strategy, among cognitive emotion regulation strategies, and the integration and compromise strategies, among conflict resolution strategies, had the double mediation effect on the association between rejection sensitivity and dating relationship satisfaction. In other words, a direct negative correlation between rejection sensitivity and dating relationship satisfation resulted in high rejection sensitivity, which was expected to generate low satisfaction in dating relationships. However, it was confirmed that the use of these two strategies-the cognitive emotion regulation and the conflict resolution strategy-could bring positive effects in terms of the dating relationship satisfaction; the former helped couples to see situations more broadly and increase positive perceptions, while the latter solve their problems each other. Based on these findings, we discuss their implications for counseling practice and therapeutic interventions