• Title/Summary/Keyword: Direct transfer

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Electrical Recognition of Label-Free Oligonucleotides upon Streptavidin-Modified Electrode Surfaces

  • Park, Jong-Wan;Jung, Ho-Sub;Lee, Hea-Yeon;Kawai, Tomoji
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.505-509
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    • 2005
  • For the purpose of developing a direct label-free electrochemical detection system, we have systematically investigated the electrochemical signatures of each step in the preparation procedure, from a bare gold electrode to the hybridization of label-free complementary DNA, for the streptavidin-modified electrode. For the purpose of this investigation, we obtained the following pertinent data; cyclic voltammogram measurements, electrochemical impedance spectra and square wave voltammogram measurements, in $Fe(CN)_6^{3-}/Fe(CN)_6^{4-}$ solution (which was utilized as the electron transfer redox mediator). The oligonucleotide molecules on the streptavidin-modified electrodes exhibited intrinsic redox activity in the ferrocyanide-mediated electrochemical measurements. Furthermore, the investigation of electrochemical electron transfer, according to the sequence of oligonucleotide molecules, was also undertaken. This work demonstrates that direct label-free oligonucleotide electrical recognition, based on biofunctional streptavidin-modified gold electrodes, could lead to the development of a new biosensor protocol for the expansion of rapid, cost-effective detection systems.

A Study on the Effects of SMVEs Export Modes on Export Amount and Period

  • Coo, Byung-Mo
    • The Journal of Industrial Distribution & Business
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    • v.9 no.1
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    • pp.39-49
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    • 2018
  • Purpose - This present aims to analyze the effect of export modes on initial export amount and time to export by selecting export modes among various success strategies and factors. Research design, data, and methodology - It surveyed 980 small and mid-sized venture enterprises across Korea. The export modes and its impact on exports through frequency analysis and cross analysis, and validated through a PPML(regression analysis applied the enterprise growth model) analysis. Results - Five export modes were investigated : direct export, indirect export, transfer from direct export to indirect export, transfer from direct export to indirect export, and parallel export to indirect export. It was found that SMVEs that exported directly from establishment to initial export had the shortest period, and also had the highest export price Conclusions - From a marketing point of view, it took an average of 1.6 years to switch from export directly to indirect export or directly export, and the reason for the export modes conversion was to supplement export specialists and improve export competitiveness. And the export amount and time period that SMVEs establishes and export is a significant factor in export success strategy and there has been few prior study in export modes.

Fabrication of Silicon Nanowire Field-effect Transistors on Flexible Substrates using Direct Transfer Method (전사기법을 이용한 실리콘 나노선 트랜지스터의 제작)

  • Koo, Ja-Min;Chung, Eun-Ae;Lee, Myeong-Won;Kang, Jeong-Min;Jeong, Dong-Young;Kim, Sang-Sig
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.413-413
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    • 2009
  • Silicon nanowires (Si NWs)-based top-gate field-effect transistors (FETs) are constructed by using Si NWs transferred onto flexible plastic substrates. Si NWs are obtained from the silicon wafers using photolithography and anisotropic etching process, and transferred onto flexible plastic substrates. To evaluate the electrical performance of the silicon nanowires, we examined the output and transfer characteristics of a top-gate field-effect transistor with a channel composed of a silicon nanowire selected from the nanowires on the plastic substrate. From these FETs, a field-effect mobility and transconductance are evaluated to be $47\;cm^2/Vs$ and 272 nS, respectively.

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Comparison of Rotor Flux Observer Characteristics in Induction Motor Using Parameter Sensitivity (파라미터 민감도를 이용한 유도전동기 회전자 자속관측기의 특성 비교)

  • 최종우;남현택;박용환;김흥근
    • The Transactions of the Korean Institute of Power Electronics
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    • v.7 no.4
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    • pp.377-383
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    • 2002
  • To obtain a high performance in a direct vector controlled induction machine, it is essential to obtain the current rotor flux. The accuracy of flux observers for induction machines inherently depends on parameter sensitivity. This paper proposes an analysis method for conventional flux observers using "parameter sensitivity". The "parameter sensitivity" is defined as the ratio of the percentage change in the system transfer function to the percentage change of the parameter variation. We define the ratio between real flux and estimated no as the transfer function, and analyze a parameter sensitivity of this transfer function. Practical verification is presented to conform the capabilities of the proposed analysis method.sed analysis method.

A Study on the Heat Recovery from Boiler Exhaust Gas with Multi-stage Water-fluidized-bed Heat Exchanger (다단 물유동층 열교환기에 의한 보일러 배가스의 폐열 회수 성능에 관한 연구)

  • Kim, Dae-Gi;Park, Sang-Il;Kim, Han-Deok
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.25 no.12
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    • pp.1776-1783
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    • 2001
  • Heat recovery from boiler exhaust gas with multi-stage water-fluidized-bed heat exchanger is analyzed in this study. The recovered energy here is not only sensible heat but also latent heat contained in the exhaust gas. In this system direct contact heat transfer occurs while exhaust gas passes through water bed and the thermal energy recovered this way is again delivered to the water circulating through heat exchanging pipes within the bed. Thus the thermal energy of exhaust gas can be recovered as a clean hot water. A computer program developed in this study can predict the heat transfer performance of the system. The results of experiments performed in this study agree well with the calculated ones. The heat and mass transfer coefficients can be fecund through these experiments. The performance increases as the number of stage increases. However at large number of stages the increasing rate becomes very low.

A Frequency-Tracking Method Based on a SOGI-PLL for Wireless Power Transfer Systems to Assure Operation in the Resonant State

  • Tan, Ping-an;He, Haibing;Gao, Xieping
    • Journal of Power Electronics
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    • v.16 no.3
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    • pp.1056-1066
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    • 2016
  • Wireless power transfer (WPT) technology is now recognized as an efficient means of transferring power without physical contact. However, frequency detuning will greatly reduce the transmission power and efficiency of a WPT system. To overcome the difficulties associated with the traditional frequency-tracking methods, this paper proposes a Direct Phase Control (DPC) approach, based on the Second-Order Generalized Integrator Phase-Locked Loop (SOGI-PLL), to provide accurate frequency-tracking for WPT systems. The DPC determines the phase difference between the output voltage and current of the inverter in WPT systems, and the SOGI-PLL provides the phase of the resonant current for dynamically adjusting the output voltage frequency of the inverter. Further, the stability of this control method is analyzed using the linear system theory. The performance of the proposed frequency-tracking method is investigated under various operating conditions. Simulation and experimental results convincingly demonstrate that the proposed technique will track the quasi-resonant frequency automatically, and that the ZVS operation can be achieved.

The study of turbulent flow structures in a wavy channel using direct numerical simulation (직접수치모사를 통한 Wavy Channel 내의 난류 유동 구조의 연구)

  • Lee, Dae-Sung;Ha, Man-Yeong;Yoon, Hyun-Sik;Chun, Ho-Hwan;Jeon, Chung-Hwan
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.1807-1812
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    • 2004
  • Sinusoidal wavy channel is one of the most commonly used devices in the industry for achieving mixing and heat transfer. Here we report on results obtained from the DNS of flow inside the wavy channel performed using the finite volume technique. As a primary stage to obtain the optimal design for heat transfer and mixing, this study observed the basic flow structures in a wavy channel. The mass flow rate is kept constant with friction Reynolds number of $Re_{\tau}$ = 140 . Time- and space-averaged and instantaneous flow fields are illustrated to observe the flow structures. Although the direct comparison of results between turbulent wavy and flat channel is somehow difficult due to the different flow phenomena derived from different configuration, here the mean streamwise velocity and RMS of velocities at same $Re_{\tau}$ of two different channels are compared. The basic difference between wavy and flat channel flow is the existence of small scale wall vortices along the walls in a wavy channel. These vortices make flow more complex, which will accompany the increase of heat transfer, pressure drop and drag.

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Electrochemical Study on the 3-Phenyl-4-Nitrosydnone (3-Phenyl-4-Nitrosydnone의 전기화학적 연구)

  • Il-Kwang Kim;Youn-Geun Kim;Soon-Jong Han
    • Journal of the Korean Chemical Society
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    • v.32 no.3
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    • pp.195-202
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    • 1988
  • An electrochemical reduction on the 3-phenyl-4-nitrosydnone in acetonitrile solution has been studied by direct current, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. Before the cleavage of phenyl-N single bond a irreversible electron transfer-chemical reaction(EC) mechanism of nitro functional group proceeded to form amino (or-hydroxylamino) group by multielectron transfer which is followed to give phenyl hydrazine by single electron transfer-chemical reaction at the 2nd and 3rd irreversible reduction wave of high negative potential region. The cathodic half-wave potentials shown to be shift negative due to inhibitory effect of cetyl-trimethyl ammonium bromide micelle while reversible anodic peaks on the 2nd and 3rd reduction waves in the presence of NaLS at high negative potential region.

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STATUS AND PERSPECTIVE OF TWO-PHASE FLOW MODELLING IN THE NEPTUNE MULTISCALE THERMAL-HYDRAULIC PLATFORM FOR NUCLEAR REACTOR SIMULATION

  • BESTION DOMINIQUE;GUELFI ANTOINE;DEN/EER/SSTH CEA-GRENOBLE,
    • Nuclear Engineering and Technology
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    • v.37 no.6
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    • pp.511-524
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    • 2005
  • Thermalhydraulic reactor simulation of tomorrow will require a new generation of codes combining at least three scales, the CFD scale in open medium, the component scale and the system scale. DNS will be used as a support for modelling more macroscopic models. NEPTUNE is such a new generation multi-scale platform developed jointly by CEA-DEN and EDF-R&D and also supported by IRSN and FRAMATOME-ANP. The major steps towards the next generation lie in new physical models and improved numerical methods. This paper presents the advances obtained so far in physical modelling for each scale. Macroscopic models of system and component scales include multi-field modelling, transport of interfacial area, and turbulence modelling. Two-phase CFD or CMFD was first applied to boiling bubbly flow for departure from nucleate boiling investigations and to stratified flow for pressurised thermal shock investigations. The main challenges of the project are presented, some selected results are shown for each scale, and the perspectives for future are also drawn. Direct Numerical Simulation tools with Interface Tracking Techniques are also developed for even smaller scale investigations leading to a better understanding of basic physical processes and allowing the development of closure relations for macroscopic and CFD models.

Quantum Secure Direct Community using Time Lag (시간지연을 이용한 양자비밀직접통신)

  • Rim, Kwang-cheol;Lim, Dong-ho
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.21 no.12
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    • pp.2318-2324
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    • 2017
  • Quantum cryptography, which is emerging as a next generation password, is being studied by quantum cryptographic transfer protocols and quantum secret communication. Quantum key transfer protocol can be used in combination with the modern password because of the inefficiency of the use of the password, or the use of OTP(one time password). In this paper an algorithm for direct communication by means of direct cryptographic communications rather than quantum keys. The method of implementing quantum secure direct community was adopted using 2-channel methods using Einstein gravity field. Two channels were designed to adopt a quantum secret communication protocol that applies time delay between 2-channels of channel to apply time difference between 2-channels. The proposed time delay effect reflects the time delay by reflecting the gravitational lensing phenomenon. Gravity generator with centrifugal acceleration is incorporated in the viscometer, and the time delay using this implies the correlation between the variance of the metametry.