• Natural Product Sciences
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• 제17권3호
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• pp.202-205
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• 2011

The Angelica root has been used as a medicinal herb in many Asian countries including Korea, China, and Japan. Angelica gigas, A. sinensis, and A. acutiloba have been considered as Angelicae radix in Korean, Chinese, and Japanese Pharmacopoeia, respectively. Since the origins of Angelicae radix differ from country to country, there is a need to develop an efficient analytical method to identify the origin of the Angelica root. In order to obtain chemical fingerprints, three different Angelicae Radices were analyzed by direct analysis in real time mass spectrometry (DART-MS). Significantly different DART-MS spectra were observed from three different species of Angelicae Radix. Strong peaks of decursin or decusinol angelate, and its dimer were exclusively found from A. gigas. Ligustilide and linoleic acid were detected as the major component from A. acutiloba. The strongest ligustilide peak was observed from A. sinensis. DART-MS fingerprinting is a promising method for the rapid identification and/or quality control of Angelicae Radix.

• Imaging Science in Dentistry
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• 제36권1호
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• pp.49-54
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• 2006

Purpose : To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. Materials and Methods : One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side. The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant differences between the direct measurements and those using the 3D images (p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. Conclusion : The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissues thickness more easily in forensic science and anthropology.

• Wind and Structures
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• 제14권5호
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• pp.413-434
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• 2011

Without output covariance estimation, one reference-based Stochastic Subspace Technique (SST) for extracting modal parameters and flutter derivatives of bridge deck is developed and programmed. Compared with the covariance-driven SST and the oscillation signals incurred by oncoming or signature turbulence that adopted by previous investigators, the newly-presented identification scheme is less time-consuming in computation and a more desired accuracy should be contributed to high-quality free oscillated signals excited by specific initial displacement. The reliability and identification precision of this technique are confirmed by a numerical example. For the 3-DOF sectional models of Sutong Bridge deck (streamlined) and Suramadu Bridge deck (bluff) in wind tunnel tests, with different wind velocities, the lateral bending, vertical bending, torsional frequencies and damping ratios as well as 18 flutter derivatives are extracted by using SST. The flutter derivatives of two kinds of typical decks are compared with the pseudo-steady theoretical values, and the performance of $H_1{^*}$, $H_3{^*}$, $A_1{^*}$, $A_3{^*}$ is very stable and well-matched with each other, respectively. The lateral direct flutter derivatives $P_5{^*}$, $P_6{^*}$ are comparatively more accurate than other relevant lateral components. Experimental procedure seems to be more critical than identification technique for refining the estimation precision.

• 한국의류산업학회지
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• 제15권1호
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• pp.12-21
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• 2013

This study examines the effectiveness of brand awareness, brand image and brand identification on brand loyalty for the Nike brand. The subjects of this study were 336 elementary school boys. The statistical methods used for this study were factor analysis, Cronbach's alpha analysis, confirmatory factor analysis and path analysis with SPSS 19.0 and AMOS 19.0. The results of this study were as follows. First, brand awareness had a direct effect on brand image and brand loyalty. Brand awareness also had an indirect effect on brand loyalty. Second, brand image directly or indirectly influenced brand loyalty as well as directly influenced brand identification. Third, brand identification directly influenced brand loyalty. The $5^{th}$ grade group and $6^{th}$ grade group had different paths; however, the most powerful path was the same as brand awareness to brand image. The results of this study will help fashion companies understand the importance of new consumer groups in their early teens or elementary school.

• 한국소음진동공학회논문집
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• 제25권12호
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• pp.830-842
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• 2015

This paper aims to develop a SI(structural identification) technique using the kinetic energy optimization technique(KEOT) and the direct matrix updating method(DMUM) to decide on optimal location of sensors and to update FE model respectively, which ultimately contributes to a composition of more effective SHM. Owing to the characteristic structural flexing behavior of cable bridges, which makes them vulnerable to any vibration, systematic and continuous structural health monitoring (SHM) is pivotal for them. Since it is necessary to select optimal measurement locations with the fewest possible measurements and also to accurately assess the structural state of a bridge for the development of an effective SHM, a SI technique is as much important to accurately determine the modal parameters of the current structure based on the data optimally obtained. In this study, the KEOT was utilized to determine the optimal measurement locations, while the DMUM was utilized for FE model updating. As a result of experiment, the required number of measurement locations derived from KEOT based on the target mode was reduced by approximately 80 % compared to the initial number of measurement locations. Moreover, compared to the eigenvalue of the modal experiment, an improved FE model with a margin of error of less than 1 % was derived from DMUM. Finally, the SI technique for long-span bridges proposed in this study, which utilizes both KEOT and DMUM, is proven effective in minimizing the number of sensors while accurately determining the structural dynamic characteristics.

• Journal of Ginseng Research
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• 제43권3호
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• pp.368-376
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• 2019

Background: In the current phytochemical research on ginseng, the differentiation and structural identification of ginsenosides isomers remain challenging. In this paper, a two-dimensional mass spectrometry (2D-MS) method was developed and combined with statistical analysis for the direct differentiation, identification, and relative quantification of protopanaxadiol (PPD)-type ginsenoside isomers. Methods: Collision-induced dissociation was performed at successive collision energy values to produce distinct profiles of the intensity fraction (IF) and ratio of intensity (RI) of the fragment ions. To amplify the differences in tandem mass spectra between isomers, IF and RI were plotted against collision energy. The resulting data distributions were then used to obtain the parameters of the fitted curves, which were used to evaluate the statistical significance of the differences between these distributions via the unpaired t test. Results: A triplet and two pairs of PPD-type ginsenoside isomers were differentiated and identified by their distinct IF and RI distributions. In addition, the fragmentation preference of PPD-type ginsenosides was determined on the basis of the activation energy. The developed 2D-MS method was also extended to quantitatively determine the molar composition of ginsenoside isomers in mixtures of biotransformation products. Conclusion: In comparison with conventional mass spectrometry methods, 2D-MS provides more direct insights into the subtle structural differences between isomers and can be used as an alternative approach for the differentiation of isomeric ginsenosides and natural products.

• 대한기계학회논문집A
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• 제36권11호
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• pp.1371-1379
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• 2012

본 논문에서는 직접분사 가솔린엔진 부품에 의해서 발생하는 진동에 대한 기여도를 분석하는 방법을 제시한다. 본 연구에서는 부분기여도함수를 적용하여 부품 상호간의 관련성에 대한 진동원을 규명 하는데 사용하였다. 직접분사 가솔린 엔진 부품의 진동원을 규명하는데 부분기여도함수 방법을 사용하기 위해서는 시스템의 모델링이 필요하며 본 연구에서는 진동 발생 경로를 2 입력과 단일 출력계로 시스템을 모델링하였다. 이 모델링을 증명 하기 위해서, 직접분사가솔린 엔진의 진동원인 고압펌프, 연료레일, 인젝터, 고압센서에 3 축 가속도계 센서로 각 부품의 진동을 측정했다. 이 모델링을 바탕으로 각각의 진동원에 대한 부분기여도 함수를 구했으며, 직접분사 부품들의 각각의 진동 기여도를 계산하였다. 부분기여도 함수를 바탕으로 한 모델링을 통해 각 부품들에서 발생되는 진동 출력 기여 값을 정량적으로 도출하였다.

• 전자공학회논문지C
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• 제35C권6호
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• pp.99-108
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• 1998

본 논문에서는 비선형 시스템의 직접제어방식을 위한 다층 신경회로망을 제안하였다. 제안한 방식은 신경회로망이 플랜트의 역 모델을 학습하는 방식으로 플랜트의 사전지식을 시스템의 입출력 정보를 이용하여 추정하고, 플랜트의 역 모델을 선형부분과 비선형 부분의 직렬연결로 구성하고 선형부분과 비선형부분의 모델을 신경회로망을 이용하여 구성한 직접제어방식이다. 제안한 제어기의 선형부분은 선형 시스템의 시스템동정을 위해 이용되었던 반복최소자승법을 이용하여 구하여진 플랜트의 선형입력으로 학습을 수행하고, 비선형부분은 기준 궤적과 실제 출력의 오차를 이용해 학습을 수행한다. 단일 관절 매니플레이터를 이용하여 추종제어에 대한 시뮬레이션과 실험을 하여 기존의 다층신경회로망을 이용한 직접제어방식과 제어성능을 비교 검토한 결과 신경회로망 구성의 간단함과 정밀성 등의 우수함을 확인하였다.

• 미생물학회지
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• 제31권4호
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• pp.292-299
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• 1993

국내에서 분리된 Agrobacterium tumefaciens KU12 는 많은 식물체에서 종양을 유발하여 octopine 을 유일한 탄소원과 질소원으로 이용할 수 있다. A. tumefaciens KU12 내에는 크기가 각각 45.5 kb, 240 kb 및 240 kb 이상인 3종류의 플라스미드가 존재하는 것으로 확인되었다. KU12내에 존재하는 octopine type Ti plasmid 를 확인하기 위하여 플라스미드를 가지고 있지 않은 A. tumefaciens A136 을 direct transformation 방법에 따라 KU12 에서 분리한 플라스미드 시료로 형질전환시킨후 질소원으로 octopine 만을 가지로 있는 AB 최소배지를 이용하여 Ti plasmid 에 의해 형질전환된 형질정환체를 선별하였다. 선별된 형질전환체를 A. tumefaciens KU911 이라고 명명하였으며 KU911 내에는 240 kb 크기의 플라스미드만이 존재하였다. 종양형성능 및 Southern hybridization 을 이용하여 octopine type Ti plasmid 인 pTiKU12 로 명명된 240 kb 크기의 플라스미드가 Ti plasmid 임을 확인되었다.

• Earthquakes and Structures
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• 제12권4호
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• pp.425-436
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• 2017

Vibration control using a tuned mass damper (TMD) is an effective technique that has been verified using analytical methods and experiments. It has been applied in mechanical, automotive, and structural applications. However, the damping of a TMD cannot be adjusted in real time. An excessive mass damper stroke may be introduced when the mass damper is subjected to a seismic excitation whose frequency content is within its operation range. The semi-active tuned mass damper (SATMD) has been proposed to solve this problem. The parameters of an SATMD can be adjusted in real time based on the measured structural responses and an appropriate control law. In this study, a stiffness-controllable TMD, called a leverage-type stiffness-controllable mass damper (LSCMD), is proposed and fabricated to verify its feasibility. The LSCMD contains a simple leverage mechanism and its stiffness can be altered by adjusting the pivot position. To determine the pivot position of the LSCMD in real time, a discrete-time direct output-feedback active control law that considers delay time is implemented. Moreover, an identification test for the transfer function of the pivot driving and control systems is proposed. The identification results demonstrate the target displacement can be achieved by the pivot displacement in 0-2 Hz range and the control delay time is about 0.1 s. A shaking-table test has been conducted to verify the theory and feasibility of the LSCMD. The comparisons of experimental and theoretical results of the LSCMD system show good consistency. It is shown that dynamic behavior of the LSCMD can be simulated correctly by the theoretical model and that the stiffness can be properly adjusted by the pivot position. Comparisons of experimental results of the LSCMD and passive TMD show the LSCMD with less demand on the mass damper stroke than that for the passive TMD.