• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.17 no.4
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• pp.367-372
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• 2004

Two new extraction methods for the thermal Resistance of HBT(Heterojunction Bipolar Transistors) are proposed. First, the analytical expression, based on the thermal characteristics that the base to emitter junction voltage drops with the increase of junction temperature, is derived. Second, the thermal resistance equation that can predict the measured DC(Direct Current) data optimally is derived. These optimal thermal resistance expression is applied to the 2 finger 2${\times}$20${\mu}{\textrm}{m}$-AlGaAs/GaAs HBT and shows the good agreement with the measured data.

• Journal of Navigation and Port Research
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• v.29 no.4
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• pp.341-347
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• 2005

A unique technique to extract the phase in time domain is proposed in order to measure the time parameters such as speed and depth by transmitting sound and electric waves. In the signal analysis processing, the phase of pulse signal can be transformed and digitalized with local data in real time without the effect of direct current bias and Nyquist limits. This method is sensitive to base frequency of pulse signal with high spacial resolution and is effective to compare two signals which have different forms. It is expected that the phase analysis technique will be applied to the measurement of the speed and depth accurately by ultrasonic pulse signal in water.

• Mycobiology
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• v.31 no.4
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• pp.196-199
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• 2003

A very reliable and specific method for the identification of fungi in ectotrophic mycorrhizal symbiosis was developed using a specific PCR assay based on the amplification of the ITS1 region. To obtain specific data, an ITS-diagnostic assay was carried out that reveals genera and species specific sequences. Here, an application of one method is presented, which covers the identification of pure mycelia, basidiocarps as well as mixed samples such as ectomycorrhizal roots that were mingled with remains of the host plant. For this purpose a protocol was established that allowed the extraction of DNA from single mycorrhizal roots. In order to perform a specific ITS analysis we generated a new ITS-primer(ITS8) by a multiple alignment of five different genera and species of mycorrhizal fungi. The utilization of ITS1 and ITS8 resulted in specific PCR amplicons, which were characterized by sequencing without purification steps, even when the template DNA was associated with roots.

• Wind and Structures
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• v.16 no.6
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• pp.561-577
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• 2013

Rational Functions are used to express the self-excited aerodynamic forces acting on a flexible structure for use in time-domain flutter analysis. The Rational Function Approximation (RFA) approach involves obtaining of these Rational Functions from the frequency-dependent flutter derivatives by using an approximation. In the past, an algorithm was developed to directly extract these Rational Functions from wind tunnel section model tests in free vibration. In this paper, an algorithm is presented for direct extraction of these Rational Functions from section model tests in forced vibration. The motivation for using forced-vibration method came from the potential use of these Rational Functions to predict aerodynamic loads and response of flexible structures at high wind speeds and in turbulent wind environment. Numerical tests were performed to verify the robustness and performance of the algorithm under different noise levels that are expected in wind tunnel data. Wind tunnel tests in one degree-of-freedom (vertical/torsional) forced vibration were performed on a streamlined bridge deck section model whose Rational Functions were compared with those obtained by free vibration for the same model.

• Journal of the Korean Chemical Society
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• v.47 no.2
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• pp.127-132
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• 2003

We have developed a direct polymerase chain reaction (PCR) and electrophoresis method for the diagnosis of bovine theileriosis from whole blood DNA analysis without DNA extraction. The technique empolyed a FoLT (formamide, low temp.) technique and was utilized in the diagnosis of bovine theileriosis. Formamide solubilize the blood cells, and the lowered incubation temperatures reduced protein coagulation. 100-200 nL of whole blood and PCR reagents were introduced directly into a PCR tube. After the amplification, the PCR product (816-bp DNA) was introduced into the electrophoresis system. The results of this analysis were consist with those obtained using purified DNA.

• Korean Journal of Veterinary Service
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• v.37 no.4
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• pp.253-261
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• 2014

Porcine circovirus disease (PCVD) is a major problem of swine industry worldwide, and diagnosis of PCV2, causal agent of PCVD, has been doing in clinical laboratories of pig disease by polymerase chain reaction (PCR) methods. But the PCR analyses have a serious problem of misdiagnosis by contamination of DNA, in particular, from carryover contamination with previously amplified DNA or extracted DNA from field samples. In this study, an uracil DNA glycosylase (UNG)-based direct PCR (udPCR) without DNA extraction process and DNA carryover contamination was developed and evaluated on PCV2 culture and field pig samples. The sensitivity of the udPCR combined with dPCR and uPCR was same or better than that of the commercial PCR (cPCR) kit (Median diagnostics, Korea) on PCV2-positive serum, lymph node and lung samples of the pigs. In addition, the udPCR method confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PCV2 DNA from previous udPCR. In clinical application, 170 pig samples (86 tissues and 84 serum) were analysed by cPCR kit and resulted in 37% (63/170) of positive reaction, while the udPCR was able to detect the PCV2 DNA in 45.3% (77/170) with higher sensitivity than cPCR. In conclusion, the udPCR developed in the study is a time, labor and cost saving method for the detection of PCV2 and providing a preventing effect for DNA carryover contamination that can occurred in PCR process. Therefore, the udPCR assay could be an useful alternative method for the diagnosis of PCV2 in the swine disease diagnostic laboratories.

• Proceedings of the Korea Information Processing Society Conference
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• 2004.05a
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• pp.761-764
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• 2004

얼굴 데이터를 사용하는 인식 시스템에서 특징 벡터의 차원은 일반적으로 매우 크다. 패턴인식에서 차원 축소는 중요한 문제로서, 효과적인 얼굴 인식을 위한 특징 벡터의 차원 축소는 필수적이라 할 수 있다. 본 논문에서는 획득된 얼굴 데이터로부터 저 차원의 강건한 특징을 얻기 위하여 웨이블릿을 사용하고, 식별력 있는 특징을 얻기 위하여 direct linear discriminant analysis를 사용하였다. Direct linear discriminant analysis 방법을 사용하기 이전에 웨이블릿을 사용함으로써 계산 복잡도를 줄여줄 뿐만 아니라 식별력을 높여주고 효과적으로 얼굴 데이터의 차원을 축소할 수 있음을 보여 준다. 얼굴의 패턴정합을 위해서는 최근접 평균 분류기(Nearest Mean Classifier)를 사용하였으며, 최근접 평균 분류기를 사용함으로써 분류를 위한 시간을 최소화하였다. 본 논문에서 인간의 얼굴인식을 위해 제시한 방법이 얼굴패턴을 표현하는 효과적인 방법이며, 시간 및 공간의 절약이라는 측면에서 유리하다는 것을 보여준다.

• Nuclear Engineering and Technology
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• v.53 no.7
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• pp.2418-2425
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• 2021

Monazite is a phosphate mineral that contains thorium (Th) and rare earth elements. The Th concentration in monazite can be as high as 500 ppm, and it has the potential to be used as fuel in the nuclear power system. Therefore, this study aimed to conduct the techno-economic analysis (TEA) of Th extraction in the form of thorium oxide (ThO₂) from monazite. Th can be extracted from monazite through an alkaline fusion method. The TEA of ThO₂ production studied parameters, including raw materials, equipment costs, total plant direct and indirect costs, and direct fixed capital cost. These parameters were calculated for the production of 0.5, 1, and 10 ton ThO₂ per batch. The TEA study revealed that the highest production cost was ascribed to installed equipment. Furthermore, the highest return on investment (ROI) of 21.92% was achieved for extraction of 1 ton/batch of ThO₂, with a payback time of 4.56 years. With further increase in ThO₂ production to 10 ton/batch, the ROI was decreased to 5.37%. This is mainly due to a significant increase in the total capital investment with increasing ThO₂ production scale. The minimum unit production cost was achieved for 1 ton ThO₂/batch equal to 335.79 $/Kg ThO₂.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.1072-1074
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• 2003

In this paper, a new control strategy of a series active power filter using direct compensating voltage extraction method is proposed. This control algorithm compensates harmonics, reactive power and neutral line currents which are generated by balanced or unbalanced nonlinear loads. The advantage of this method is that the compensating voltage of the series active power filter can be extracted without phase transformation. Therefore, calculation time is shorten and the control method is simple compared with conventional method as the p-q theory In addition, this control strategy was applied for the series active power filter in 3-phase 4-wire system which is widely employed in distributing electric energy to several office building and manufacturing plants. Some results obtained from the experimental model using the proposed method are presented to demonstrate and confirm its validity.

• Korean Journal of Food Science and Technology
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• v.33 no.3
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• pp.282-287
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• 2001

This study was carried out to investigate the difference of ginsenoside compositions in crude ginseng saponins prepared by five different methods including three new methods. Two known methods are hot methanol(MeOH) extraction/n-butanol(n-BuOH) fractionation and hot MeOH extraction/Diaion HP-20 adsorption/MeOH elution. Three new methods are hot MeOH extraction/cation AG 50W $absorption/H_2O$ elution/n-BuOH extraction, cool MeOH extraction/Diaion HP-20 adsorption/MeOH elution and direct extraction with ethyl acetate(EtOAc)/n-BuOH. Analysis of ginsenoside composition in the crude saponins by conventional HPLC/RI(Refractive Index) did not show great difference between methods except EtOAc/n-BuOH method. However, HPLC/ELSD (evaporative light scattering detector) employing gradient mobile phase afforded fine resolution of ginsenoside Rf, $Rg_1$ and $Rh_1$, and great difference of ginsenoside compositions between methods. LC/MS revealed that large amount of prosapogenins were produced during the pass through the cation exchange (AG 50W) column being strongly acidic. Six major ginsenosides such as $Rb_1,w;Rb_2,$ Rc, Rd, Re and $Rg_1$, 5 prosapogenins and one chikusetsusaponin were identified by LC/MS. A newly established HPLC method employing ODS column and gradient mobile phase of $KH_2PO_4/CH_3CN$ revealed that malonyl ginsenosides were detected only in the crude saponin obtained from cool MeOH extraction.