• BMB Reports
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• v.29 no.1
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• pp.63-67
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• 1996

In order to elucidate the molecular mechanism of plasmid incompatibility of broad host-range plasmid R1162, the plasmid-encoded replication protein RepIB was purified and tested for binding to the 20 bp direct repeat (DR) DNA sequence which is reiterated 3 and 1/2 times within the replicative origin of the plasmid. The RepIB protein specifically binds to the DR DNA. Point mutations in the DR which affect expression of plasmid incompatibility also coordinately affect binding. These results indicate that the incompatibility of broad host-range plasmid R1162 is exerted by the DR DNA by titrating the essential replication protein RepIB.

• Journal of Korea Multimedia Society
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• v.13 no.7
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• pp.933-942
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• 2010

The convergence of the telecommunication industry through the combination of existing network technology and multimedia technology has brought along interactive data services in addition to the traditional broadcast services by providing direct connections between the users and the broadcasters. The interactive data service technology has developed a network which is able to fully support QoS and also a fast enough channel changing technology that is satisfactory to the user, are prerequisites for IPTV success, to provide satisfactory levels of service to exist users. However the current server-client system can't support all increasing needs of users. To overcome these limitations, P2P based IPTV service is currently under researched. Therefore, this thesis proposes a logical view based copy-ahead technique for P2P IPTV to improve the performance of IPTV system.

• Journal of the Korean Vacuum Society
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• v.19 no.2
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• pp.96-104
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• 2010

We suggest here a cost-effective replica fabrication method for transparent and hard molds for imprinting lithography such as NIL and S-FIL. The process starts with the use of a replica hard mold from a master, using a polymer copy as a carrier. The polymer copy as a carrier was treated by soluble process for forming anti-adhesion layer. Duplicated hard molds can eliminate direct contact between a hard master and a patterned polymer on a substrate and the generated contamination of a master during the imprinting process. The replica hard mold exhibits the glass-like properties introduced here, such as transparency and hardness, make it appropriate for nanoimprint lithography and step-and-flash imprint lithography.

• BMB Reports
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• v.37 no.3
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• pp.351-355
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• 2004

Separate protocols are commonly used to prepare plasmid DNA, chromosomal DNA, or total RNA from E. coli cells. Various methods for the rapid preparation of plasmid DNA have been developed previously, but the preparation of the chromosomal DNA and total RNA are usually laborious. We report here a simple, fast, reliable, and cost-effective method to extract total nucleic acids from E. coli by direct lysis of the cells with phenol. Five distinct and sharp bands, which correspond to chromosomal DNA, plasmid DNA, 23S rRNA, 16S rRNA, and a mixture of small RNA, were observed when analyzing the prepared total nucleic acids on a regular 1-2% agarose gel. The simple and high-quality preparation of the total nucleic acids in a singe tube allowed us to rapidly screen the recombinant plasmid, as well as to simultaneously monitor the change of the plasmid copy number and rRNA levels during the growth of E. coli in the liquid medium.

• Journal of Ginseng Research
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• v.41 no.4
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• pp.469-476
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• 2017

Background: Panax ginseng Meyer (Asian ginseng) has a large nuclear genome size of > 3.5 Gbp in haploid genome equivalent of 24 chromosomes. Tandem repeats (TRs) occupy significant portions of the genome in many plants and are often found in specific genomic loci, making them a valuable molecular cytogenetic tool in discriminating chromosomes. In an effort to understand the P. ginseng genome structure, we characterized an ultrahigh copy 167-bp TR (Pg167TR) and explored its chromosomal distribution as well as its utility for chromosome identification. Methods: Polymerase chain reaction amplicons of Pg167TR were labeled, along with 5S and 45S rDNA amplicons, using a direct nick-translation method. Direct fluorescence in situ hybridization (FISH) was used to analyze the chromosomal distribution of Pg167TR. Results: Recently, we reported a method of karyotyping the 24 chromosome pairs of P. ginseng using rDNA and DAPI (4',6-diamidino-2-phenylindole) bands. Here, a unique distribution of Pg167TR in all 24 P. ginseng chromosomes was observed, allowing easy identification of individual homologous chromosomes. Additionally, direct labeling of 5S and 45S rDNA probes allowed the identification of two additional 5S rDNA loci not previously reported, enabling the refinement of the P. ginseng karyotype. Conclusion: Identification of individual P. ginseng chromosomes was achieved using Pg167TR-FISH. Chromosome identification is important in understanding the P. ginseng genome structure, and our method will be useful for future integration of genetic linkage maps and genome scaffold anchoring. Additionally, it is a good tool for comparative studies with related species in efforts to understand the evolution of P. ginseng.

• Proceedings of the Korean Information Science Society Conference
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• 1998.10c
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• pp.234-236
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• 1998

본 논문은 GUI(Graphical User interface) 환경에서 MPGE-1 영상 편집을 위한 시스템 도구(Tool)를 기술한다. 기존에 출시된 제품은 일반적으로 AVI 나 MOV 포맷(format)을 사용하는데 비하여 본 논문에서는 MPGE-1을 압축 영역 상태에서 편집함으로써 영상의 품질과 저장의 효율성 및 처리속도에 많은 이점을 두었다. 편집 작업은 Random Access 의 기본 단위가 되는 GOP(Group of Picture) 단위의 경계 편집을 사용하여 영상 편집 기능인 Copy, Paste, Cut, Undo등을 기술하였다. 또한 효율적인 편집 환경을 위해 편집 윈도우 형태를 클립 청, 클립 정보 창, 편집 창, 재생 창, 미리 보기 창으로 구분하였다. 편집된 영상은 효율적 재생을 위하여 DirectDraw을 사용하였다. 본 논문에서 소개한 편집 시스템은 GUI 기반으로 사용자에게 익숙한 사용자 인터페이스 환경을 기술하였다.

• Journal of Korea Society of Digital Industry and Information Management
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• v.5 no.4
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• pp.51-59
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• 2009

Many failures are due to incorrectly programmed transactions and data entry errors. System failure causes the loss or corruption of the contents of volatile storage. Although global processing protects data values to detect direct or indirect information effluence, security environments are very important in the recovery management of heterogeneous systems. Although transaction can't control system fault, the restart for the system can cause information effluence by low bandwith. From various faults, it is not easy to maintain the consistency and security of data. This paper proposes recovery management protocols to assure global multilevel secure one-copy quasi-serializability in security environments of heterogeneous systems with replicated data and proves its correctness. The proposed secure protocols guarantee the reliability and security of system when the system fault is happened.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.6 no.8
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• pp.73-79
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• 1983

Industrial management can be achieved in the improvement of productivities and goods qualities, only copy with international competition through technological renovation, This compels the renewal and augment of production equipments, for which more investment has to be projected. Equipment investment, However, has the possibility of assuring the expectation of profit, but the capricious reality of economical situation caused by inflation requires the precedent study of exmining, analyzing and assaying the effect of equipment investment for a long term. The resolution must depend on a technique of what is called engineering economy, which scrutinizes some samples of investment analysis under price fluctuations, which designates through a method of direct calculation that the only less scale of primal investment never bestows wider profit, and recognizes what contribution engineering economy has to the decision making of management.

• Proceedings of the KIEE Conference
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• 1998.07g
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• pp.2427-2429
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• 1998

Digital watermarking is the techinque which embeds the invisible signal into multimedia data such as audio, video, images, for copyright protection, including owner identification and copy control information. This paper proposes a new watermark embedding and extraction technique by extending the direct sequence spread spectrum technique. The proposed technique approximates the frequency component of pixels in spatial domain by using Laplacian mask and adaptively embeds the watermark considering the HVS to reduce the degradation of Image. In watermark extraction process, the proposed technique strengthens the high frequency components of image and extracts the watermark by demodulation. All this processes are performed in spatial domain to reduce the processing time.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.455-467
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• 2021

Macrofungi are valuable resources as novel drug candidates, new biomaterials, and edible materials. Recently, genetic approaches pertaining to macrofungi have been continuously growing for their identification, molecular breeding, and genetic engineering. However, purification and amplification of fungal DNA is challenging because of the rigid cell wall and presence of PCR inhibitory metabolites. Here, we established a direct PCR method to provide a rapid and efficient method for PCR-grade macrofungal DNA preparation applicable to both conventional PCR and real-time PCR. We first optimized the procedure of lysis and PCR using the mycelia of Lentinula edodes, one of the most widely consumed macrofungal species. Lysates prepared by neutralizing with (NH₄)₂SO₄ after heating the mycelia in a mixture of TE buffer and KOH at 65℃ for 10 min showed successful amplification in both conventional and real-time PCR. Moreover, the addition of bovine serum albumin to the PCR mixture enhanced the amplification in conventional PCR. Using this method, we successfully amplified not only internal transcribed spacer fragments but also low-copy genes ranging in length from 500 to 3,000 bp. Next, we applied this method to 62 different species (54 genera) of macrofungi, including edible mushrooms, such as Pleurotus ostreatus, and medicinal mushrooms such as Cordyceps militaris. It was found that our method is widely applicable to both ascomycetes and basidiomycetes. We expect that our method will contribute to accelerating PCR-based approaches, such as molecular identification, DNA marker typing, gene cloning, and transformant screening, in macrofungal studies.