• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.2B
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• pp.129-137
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• 2003

Multimedia streams, like MPEG continuously retrieve multimedia data because of their incessant playback. While these streams need an efficient support of kernel, the current buffer cache mechanism of Linux kernel such as Unix operating system was designed apt for small files, which is aperiodically requested as well as time uncritical. But, in case of continuous media, the CPU must enormously copy memory from kernel address space to user address space. This must lead to a large CPU overhead. This overhead both degrades system throughput and cannot guarantee QOS. In this paper, we have designed and implemented two memory copy reduction schemes in Linux kernel, direct I/O and one copy. The direct I/O skips the buffer cache layer of Linux kernel and results in dramatic reduction of CPU memory copy overhead. And, the one copy provides a fast disk-to-network data path without copying to user address space. The experimental results show considerable reduction of CPU overhead and throughput improvements.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.144-151
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• 2014

Increasing the gene copy number has been commonly used to enhance the protein expression level in the yeast Pichia pastoris. However, this method has been shown to be effective up to a certain gene copy number, and a further increase of gene dosage can result in a decrease of expression level. Evidences indicate the gene dosage effect is product-dependent, which needs to be determined when expressing a new protein. Here, we describe a direct detection of the gene dosage effect on protein secretion through expressing the enhanced green fluorescent protein (EGFP) gene under the direction of the ${\alpha}$-factor preprosequence in a panel of yeast clones carrying increasing copies of the EGFP gene (from one to six copies). Directly examined under fluorescence microscopy, we found relatively lower levels of EGFP were secreted into the culture medium at one copy and two copies, substantial improvement of secretion appeared at three copies, plateau happened at four and five copies, and an apparent decrease of secretion happened at six copies. The secretion of EGFP being limiting at four and five copies was due to abundant intracellular accumulation of proteins, observed from the fluorescence image of yeast and confirmed by western blotting, which significantly activated the unfolded protein response indicated by the up-regulation of the BiP (the KAR2 gene product) and the protein disulfide isomerase. This study implies that tagging a reporter like GFP to a specific protein would facilitate a direct and rapid determination of the optimal gene copy number for high-yield expression.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.237-242
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• 2007

Genome duplication(i.e. polyploidy) is a common phenomenon in the evolution of plants. The objective of this study was to achieve a comprehensive understanding of genome duplication for SNP discovery by Thymine/Adenine(TA) cloning for confirmation. Primer pairs were designed from 793 EST contigs expressed in the roots of a supernodulating soybean mutant and screened between 'Pureunkong' and 'Jinpumkong 2' by direct sequencing. Almost 27% of the primer sets were failed to obtain sequence data due to multiple bands on agarose gel or poor quality sequence data from a single band. TA cloning was able to identify duplicate genes and the paralogous sequences were coincident with the nonspecific peaks in direct sequencing. Our study confirmed that heterogeneous products by the co-amplification of a gene family member were the main cause of obtaining multiple bands or poor quality sequence data in direct sequencing. Counts of amplified bands on agarose gel and peaks of sequencing trace suggested that almost 27% of nonrepetitive soybean sequences were present in as many as four copies with an average of 2.33 duplications per segment. Copy numbers would be underestimated because of the presence of long intron between primer binding sites or mutation on priming site. Also, the copy numbers were not accurately estimated due to deletion or tandem duplication in the entire soybean genome.

• Journal of Korean Public Health Nursing
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• v.6 no.2
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• pp.58-69
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• 1992

The purposes of this study were 1) to assess the currunt documentation system 2) to identify the problems in communication regarding to documentation 3) to develop new documentation system 4) to suggest effective communication channel using new documentation system Research was conducted by direct observation, chart review, staffs interview and servey. Results were as follows: 1) nursing care plans were not used in ongoing care 2) documentation format was primarily narrative and charting was time consuming 3) documentation did not reflect the nursing process 4) patient records were not used as effective communication tool between case manager and part time nurse 5) difficult access to patient record for nurse manager created inefficiency in coordinating 6) documentation of patient education did not describe the precise contents of education, and the responses of the patients and evaluation To solve these problems, new documentation format was developed. With new formats nurses : 1) use standardized care plan which contains nursing diagnosis, ecpected outcome, time frame for evaluation, flow sheet for updating the plans 2) leave one copy of care plan at patient home for mutual agreement with patent and communication among nursing staffs 3) carry one copy of care plan for updating 4) document and evaluate the patient education using education check list keeping in patient's home 5) document nursing process in focus charting visit report 6) carry one copy of visit report 7) have one copy of visit report which was deligated to part time nurses 8) use documentation in direct communication with part time nurse 9) use beeper and memo to promote communication

• Journal of Conservation Science
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• v.34 no.6
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• pp.471-479
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• 2018

Seonunsa Temple is a site of Dancheong cultural significance in Daewoongjeon. Three-dimensional (3D) scanning can be used to create a simulation in 1:1 ratio without touching the Dancheong patterns directly. The traditional method of recreating Dancheong was paining with tracing paper using a fix pin. However, manual direct copy processes can cause and damage to the objects. This study shows the results of a simulation of a tranditional Dancheong patterns as an alternative; the simulation was able to reduce dimensional errors and prevent damage by using 3D scanning. As a result, objective and precise proportions of the simulation were acquired. The 3D scanning method may be applied for work such as the replication and restoration of the drawing, 3D fabrication of the original data, and printing of the additional drawing. In addition, with the production of 3D materials, a virtual museum is possible.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• 한국정보통신설비학회:학술대회논문집
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• 2004.08a
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• pp.137-141
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• 2004

네트워크 사용자의 급속한 증가로 네트워크 내의 부하를 감당하기에는 많은 어려움을 가져왔다. 이와 같은 이유로 기존의 TCP/IP에서 세션을 통하여 노드들 간의 통신을 연결하는 방식에서 현재는 하나의 채널을 통해 고속의 I/O가 가능하도록 하는 기술이 많이 연구되고 있다. 그 대표적인 것으로 인피니밴드가 있다. 인피니밴드는 프로세싱 노드와 입출력 장치 사이의 통신, 프로세스간 통신에 대한 산업 표준이 되고 있고 프로세싱 노드와 입출력 장치를 연결하기 위해 스위치 기반의 상호 연결은 전통적인 버스 입출력을 대체하는 새로운 입출력 방식이 사용된다. 또한 인피니밴드에서는 현재 많은 이슈가 되고 있는 RDMA 방식을 이용해 원격지 서버들 간에 직접 메모리 접근 방식을 통해 CPU와 OS의 로드를 최소화하고 있다. 본 논문에서는 RDMA를 적용한 새로운 채널 기반 네트웍의 프로토콜인 SDP(Socket Direct Protocol)를 구현하여 SDP_STREAM의 패킷 처리량에 대한 성능을 평가한다. 그리고 이에 대한 성능 평가를 위해서 Netperf 툴을 이용했다. 특히 Zero-Copy방식을 사용하지 않는 일반적인 소켓 API을 이용한 TCP_STREAM과 Zero-Copy방식을 이용한 SDP_STREAM의 패킷 처리량을 비교했으며 성능 평가 결과는 기존의 TCP_STREAM 패킷 처리량에 비해 약 3배 이상 향상된 결과를 나타냈다.

• Journal of Information Technology Applications and Management
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• v.24 no.1
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• pp.1-10
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• 2017

Recently, the problem of plagiarism has emerged as a big social issue because not only literature but also thesis become the target of plagiarism. Even the government requires conformation for plagiarism of high-ranking official's thesis as a standard of their ethical morality. Plagiarism is not just direct copy but also paraphrasing, rewording, adapting parts, missing references or wrong citations. This makes the problem more difficult to handle adequately. We propose a plagiarism detection scheme called a bit signature in which each unique word of document is represented by 0 or 1. The bit signature scheme can find the similar documents by comparing their absolute and relative bit signatures. Experiments show that a bit signature scheme produces better performance for document copy detection than existing similar schemes.

• Journal of Korea Society of Digital Industry and Information Management
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• v.5 no.4
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• pp.99-108
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• 2009

In the security environments of heterogeneous multidatabase systems, not only the existing local autonomy but also the security autonomy as a new constraint are required. From global aspects, transactions maintain consistent data value when they assure serializability. Also, secure properties must protect these transactions and data values to prevent direct or indirect information effluence. This paper proposes scheduling algorithm for global transactions to ensure multilevel secure one-copy quasi-serializability (MLS/1QSR) in security environments of multidatabase systems with replicated data and proves its correctness. The proposed algorithm does not violate security autonomy and globally guarantees MLS/1QSR without indirect information effluence in multidatabase systems.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.4
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• pp.91-99
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• 2014

The main game architectures for multiplayer online games are the traditional client-server architectures, multi-server architectures and P2P(peer-to-peer) architectures. P2P architectures, due to their distributed and collaborative nature, have low infrastructure costs and can achieve high scalability as well as fast response time by creating direct connections between players. However, P2P architectures face many challenges. Distributing a game among peers makes maintaining control over the game more complex. These architectures also tend to be vulnerable to churn and cheating. Providing consistency control in P2P systems is also more difficult since conflicting updates might be executed at different sites resulting in inconsistency. In order to avoid or correct inconsistencies, most multiplayer games use a primary-copy replication approach where any update to the object has to be first performed on the primary copy. This paper presents the primary-copy model with the update dissemination mechanism that provides consistency control over an object in P2P architectures for multiplayer online games. The performance for this model is evaluated through simulation experiments and analysis.