• Mycobiology
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• v.50 no.6
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• pp.439-447
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• 2022

Two strains, YP344 and YP579 were isolated from soil samples in Pocheon City, Gyeonggi Province, South Korea. The strains YP344 and YP579 belong to the genus Vishniacozyma and Dioszegia, respectively. The molecular phylogenetic analysis showed that the strain YP344 was closely related to Vishniacozyma peneaus. Strain YP344T differed by four nucleotide substitutions with no gap (0.70%) in the D1/D2 domain of the LSU rRNA gene and 16 nucleotide substitutions with 8 gaps (5.76%) in the ITS region. On the other hand, the strain YP579^T varied from the type strain of the most closely related species, Dioszegia zsoltii var. zsoltii, by 6 nucleotide substitutions with four gaps (1.64%) in the D1/D2 domain of LSU rRNA gene and 26 nucleotide substitutions with 14 gaps (8.16%) in the ITS region. Therefore, the name Vishniacozyma terrae sp. nov. and Dioszegia terrae sp. nov. are proposed, with type strains YP344T (KCTC27988^T) and YP579^T (KCTC 27998^T), respectively.

• The Korean Journal of Mycology
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• v.45 no.1
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• pp.23-30
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• 2017

Twenty-two yeast strains of 15 species were isolated from the leaves of 20 trees on Oknyeobong Peak, and 24 yeast strains of 12 species were isolated from the leaves of 20 trees on Yeonjasan Mountain in Daejeon city, Korea. Cryptococcus bestiolae (5 strains) and Aureobasidium pullulans (8 strains) were the predominant isolates from Oknyeobong Peak and Yeonjasan Mountain, respectively. Of a total of 46 yeast strains, Cryptococcus kuetzingii JSL508, Cryptococcus vishniacii JSL509, and Dioszegia takashimae JSL510 from Okyeobong Peak, and Plowrightia periclymeni JSL514, Erythrobasidium hasegawanium JSL0193, and Rhodotorula nothofagi JSL0196 from Yeonjasan Mountain were determined to be yeast strains that were yet unrecorded in Korea. Morphological and cultural characteristics of these unrecorded yeasts were investigated. Erythrobasidium hasegawanium JSL0193 and Rhodotorula nothofagi JSL0196 did not form ascospores and pseudomycelia. All the strains, except Dioszegia takashimae JSL510, were halotolerant or halophilic, and Cryptococcus kuetzingii JSL508 and Dioszegia takashimae JSL510 were thermophilic, growing at $37^{\circ}C$.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.67-79
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• 2021

This study aimed to isolate wild yeasts from waters and soils on the riversides of Geumgang upstream in Geumsan, seashores of Cheongpodae in Taean and Suncheonman in Suncheon, Korea, and to investigate their unrecorded microbiological characteristics. Forty-nine species of wild yeasts, including Aureobasidium pullans YH 4-3, were isolated from 40 samples of Cheonnaegang in Geumgang upstream. Fifty-six species and 36 species of wild yeasts were also isolated from 80 samples of Cheongpodae Beach and Suncheonman Mashland, respectively. Species from Candida genus were isolated from all three locations. Among them, Candida michaelii NNIBRFG28278, Sporobolomyces japonicus NNIBRFG28271, Dioszegia buhagiarii NNIBRFG28279, Ustilago spermophora NNIBRFG28273, Nakazawaea pomicola NNIBRFG31590, Candida natalensis NNIBRFG31591, Candida pseudorhagii NNIBRFG31592, Candida santamariae NNIBRFG31593, Cutaneotrichosporon terricola NNIBRFG31594, and Meira nashicola NNIBRFG31595 represent newly recorded yeast strains in Korea. Almost all of these unrecorded yeasts were oval shaped, and Sporobolomyces japonicus NNIBRFG28271 and Candida natalensis NNIBRFG31591 have ascospores. All the strains grew well in yeast extract-peptone-dextrose (YPD) and yeast extract-malt extract (YM) media, and Ustilago spermophora NNIBRFG28273 grew well in vitamin-free medium. Sporobolomyces japonicus NNIBRFG28271 grew well in 15% NaCl-containing YPD medium, and Ustilago spermophora NNIBRFG28273 and Dioszegia buhagiarii NNIBRFG28279 assimilated starch, and Ustilago spermophora NNIBRFG28273 produced ethanol.

• The Korean Journal of Mycology
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• v.42 no.3
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• pp.231-234
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• 2014

Six unrecorded yeasts, Cryptococcus festucosus 41-3, Cryptococcus heveanensis 56-4, Debaryomyces nepalensis 95-4, Issatchenkia occidentalis 142-1, Dioszegia zsoltii 39-1, and Kwoniella europala 47-2 were screened from 108 yeasts isolated from flowers and fruits in orchards of Yesan-gun, Chungcheongnam-do, Korea. The morphological and cultural characteristics of these unrecorded yeasts were investigated. They had various shapes, including ellipsoidal, globose, and oval, and also had budding mode in vegetable reproduction, except I. occidentalis 142-1 (fission mode). K. europaea 47-2 only formed pseudomycelium. D. zsoliti 39-1 did not grow in yeast extract-malt extract medium, potato dextrose medium, and vitamin-free medium. C. festucosus 41-3 grew well in 5% NaCl-containing yeast extract-peptone-dextrose medium and had a growth pH range of 7.0~10.0. Three unrecorded yeasts Ogataea polymorpha HB45-1, Rhodotonula hinnulla HB62-2, and Cryptococcus rajasthanensis HB80-4 were screened from 51 yeasts isolated from flowers in Hanbat arboretum in Daejeon city, Korea. They were globose in shape and did not form pseudomycelium. In addition, O. polymorpha HB45-1 and C. rajasthanensis HB80-4 had budding mode in vegetable reproduction. All of them grew well in vitamin-free medium and C. rajasthanesis HB80-4 also grew in 50% glucose and 5% NaCl-containing YPD medium.