• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4291-4295
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• 2015

Background: Chemokines and their receptors influence carcinogenesis and cysteine-cysteine chemokine receptor 5 (CCR5) directs spread of cancer to other tissues. A 32 base pair deletion in the coding region of CCR5 that might alter the expression or function of the protein has been implicated in a variety of immune-mediated diseases. The action of antiviral drugs being proposed as adjuvant therapy in cancer is dependent on CCR5 wild type status. In the present study, distribution of CCR5${\Delta}32$ polymorphism was assessed in North Indian esophageal cancer patients to explore the potential of using chemokine receptors antagonists as adjuvant therapy. Materials and Methods: DNA samples of 175 sporadic esophageal cancer patients (69 males and 106 females) and 175 unrelated healthy control individuals (69 males and 106 females) were screened for the CCR5${\Delta}32$ polymorphism by direct polymerase chain reaction (PCR). Results: The frequencies of wild type homozygous (CCR5/CCR5), heterozygous (CCR5/${\Delta}32$) and homozygous mutant (${\Delta}32/{\Delta}32$) genotypes were 96.0 vs 97.72%, 4.0 vs 1.71% and 0 vs 0.57% in patients and controls respectively. There was no difference in the genotype and allele frequencies of CCR5${\Delta}32$ polymorphism in esophageal cancer patients and control group. Conclusions: The CCR5${\Delta}32$ polymorphism is not associated with esophageal cancer in North Indians. As the majority of patients express the wild type allele, there is potential of using antiviral drug therapy as adjuvant therapy.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제3권6호
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• pp.231-236
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• 2014

의료 초음파 영상에서 조영제의 전이시간과 조영효과 변화 곡선 특성에 대한 파라미터는 각종 소화기 질환을 진단하는 중요한 인자가 된다. 이러한 진단인자들에 대한 육안판별의 어려움을 극복하기 위하여 파라미터 영상의 자동 생성 기법을 구현할 수 있는데 이 과정에서 마이크로 버블형태의 노이즈와 호흡에 의한 흔들림 현상은 추출된 영상의 신뢰도를 저하 시킨다. 이에 본 연구에서는 MRF(Markov Random Field) 모델을 기반으로 하는 최적화 기법을 적용하여 파라미터 영상을 개선하는 방법을 고찰하며, 호흡에 의한 영상의 흔들림을 보정하기 위한 영상추적 기법을 제시한다. 세부적으로 초음파 동영상 원시 데이터로부터 호흡주기 추출 기법을 구현하였으며, 추출된 주기를 기반으로 모멘텀 요소와 동적 가중치를 반영하는 ROI(Region of Interest) 추적 알고리즘을 적용하였다. 또한 영상 개선 기법에 적용되는 Gibbs 샘플러의 에너지 함수를 정의하고 실제 간질환 진단 데이터를 대상으로 영상 개선 효과를 실험적으로 평가하였다.

• 대한한방소아과학회지
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• 제24권1호
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• pp.1-8
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• 2010

Objectives This study is to find out how fungiform papillae count, which represents the density of taste buds, is related to eating behavior of children and adolescents. Methods The authors measured fungiform papillae count, height, and weight of 50 healthy children aged from 2 to 15. To evaluate the severity of anorexia, parents of the subjects answered modified version of Korean Children's Eating Behaviour Questionnaire (K-CEBQ). Children with weight of less than 50th percentile were assigned to low-weight group, while the others to high-weight group. Pearson's correlation test was conducted in order to evaluate the relationship between modified K-CEBQ score and fungiform papillae count. Results Low-weight children scored $29.8{\pm}9.1$, while high-weight children scored $24.5{\pm}7.1$. (p<0.05) on modified K-CEBQ Pearson's correlation coefficient between modified K-CEBQ score and fungiform papillae count was 0.503 (p<0.05) in low-weight group, 0.260 in high-weight group, and 0.339 (p<0.05) in total. However, there were no statistical significance in correlations between modified K-CEBQ score and percentile of weight, height, or BMI. Conclusions Severity of anorexia was correlated to the density of taste buds, especially in children who weighed less than average. The analysis on each single question indicated that children with high taste bud density had poor appetite not because of their inadequate digestive function, but because of their fastidious eating habit. Further study should be focused on finding out which specific aspect of appetite is related to the taste bud density.

• Biomolecules & Therapeutics
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• 제5권4호
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• pp.369-375
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• 1997

General pharmacological effects of SB-31$^{R}$, the extracts of Pulsatilla koreana, were investigated in mice, rats and guinea-pigs. Intravenous injection of SB-31 (3 and 6 ml/kg) produced almost no effect on central nervous system no effects on the general symptom and behaviors of mice, spontaneous locomotor activity, pentobarbital- induced sleeping time , rotared performance , electroshock and pentylenetertrazole -induced seizures, acetic acid-induced writhing and normal body temperature in mice. SB-31 showed little effects on the spontaneous movement of the isolated ileum and contraction induced by agonists in isolated ileum, suggesting no influence on autonomic nervous system. Administration of SB-31 also did not show any effect on blood pressure in conscious rats. However, a slight decrease in heart rate was observed at high doses (6 and 10 ml/kg) of SB-31 in conscious rats. Similarly, a slight increase in respiratory rate was observed at 6 m1/kg of SB-31 in anesthetized rats. SB-31 did not produce any effect at the dose of 3 ml/kg, but showed a tendency to increase the urinary volume at 6 ml/kg, and produced a decrease in urinary excretions of N $a_{+}$and $K_{+}$at 6 ml/kg. However, transport capacity within the gastrointestinal tract and the secretion of the gastric juice were not influenced by 6 ml/kg of SB-31. In conclusion, these results suggest that SB-31 did not pro-duce any acute effects on the central nervous system, autonomic nervous system, respiratory and circulatory systems, digestive system and kidney function at the dose of below 3 ml/kg.ml/kg.

• Biomolecules & Therapeutics
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• 제20권1호
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• pp.89-95
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• 2012

Poncirus trifoliata fruit (PTF) affects the digestive and cardiovascular systems, and kidney function. The authors studied the effects of ethyl acetate (EtOAc) extract of PTF on the activities of osteoblasts and in an animal model. The main compounds of the EtOAc extract, naringin and poncirin have been confirmed by HPLC and NMR analysis. Effects of osteoblastic differentiation were measured by alkaline phosphatase (ALP) activity, osteopontin (OPN) protein expression and osteoprotegerin (OPG) mRNA expression in MC3T3-E1 cells. Also, osteoclast differentiation was measured by multinucleated cells (MNCs) formation through tartrate resistance acid phosphatase (TRAP)-positive staining. Bone mineral density (BMD) was measured before and after treatment with EtOAc extract of PTF in prednisolone-induced osteoporotic mice. Dexamethasone (DEX) decreased OPN and OPG expression level in MC3T3-E1 cells and ALP activity was decreased by DEX dose-dependently. EtOAc extract of PTF recovered the levels of ALP activity, and the expression of OPN and OPG in MC3T3-E1 cells treated with DEX. In osteoclast differentiation, multinucleated TRAP-positive cell formation was significantly suppressed by the EtOAc extract of PTF. Total body BMD was restored by EtOAc extract of PTF in prednisolone-induced osteoporotic mice. In conclusion, EtOAc extract of PTF recovered DEX-mediated deteriorations in osteoblastic and osteoclastic functions, and increased BMD in glucocorticoid-induced osteoporosis.

• Journal of Microbiology
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• 제45권5호
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• pp.373-378
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• 2007

Based on observations that lactic acid bacteria have the ability to activate macrophages, we assessed the potential effects of eight different Lactobacillus strains treated with gastrointestinal enzymes on the production of nitric oxide and various cytokines in macrophages. RAW 264.7 murine macrophage cells were cultured with either precipitates or supernatants of Lactobacillus strains digested with pepsin followed by pancreatin. The increased production of nitric oxide and interleukin $(IL)-1{\beta}$, IL-6, IL-12 and tumour necrosis factor $(TNF)-{\alpha}$ were observed when cultured with precipitates, and this effect was largely strain-dependent. In contrast, the exposure of RAW 264.7 cells to supernatants produced weaker or nearly undetectable effects in comparison to the effects of exposure to precipitates. The induction of nitric oxide appeared to be unaffected. These results demonstrate that nitric oxide and cytokines were effectively induced when the bacterial precipitate was treated with macrophages. The results of the present study also indicate that Lactobacillus strains treated with digestive enzymes are capable of stimulating the production of nitric oxide and cytokines in macrophages, which may modulate the gastrointestinal immune function of the host when it is given as a feed additive.

• 대한한방소아과학회지
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• 제19권2호
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• pp.255-269
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• 2005

Objective : To evaluate the effect of Oriental Medical Treatment on a patient with Hypoxic Ischemic Encephalopathy followed by Cardiopulmonary Resuscitation Method : We applied various methodology of Oriental Medical Treatment including Acupuncture, Electroacupuncture, Physical treatment, Herbal Medicine, Moxibustion treatment and Western medication as well. Result: Herbal medicine was applied on the basis of the patient's history. We applied formular to remove phelgm as a pathogenic factor after Hypoxic Ischemic Encephalopathy. At the same time, considering the patient spent more than a month in ICU lacking appropriate nutrition, we used formuli on the basis of 'Deficiency of Spleen' focusing to vitalize the function of digestive system. As the condition of the patient changed, we also adapted formular accordingly. We prescribed Herbal medication to strengthen Yin and Yang equally as she got hospitalized for long time. Also we applied Acupuncture treatment and Moxibustion treatment to control Qi flow. The general condition of the patient got better with successful removal of Foley catheter and elevated Glasgow Coma scale. We used Electroacupuncture, Physical treatment and Western medication at the same to get maximized effect on relaxing the contracted muscle. According to the Modified Ashworth Scale (MAS), we have some changes in muscle spasticity but later, the effect was not that significant. Conclusion : We had a patient with Hypoxic Ischemic Encephalopathy followed by Cardiopulmonary Resuscitation. In the management of Hypoxic Ischemic Encephalopathy, Conservative treatments are the mainstream but there are not many alternatives. Therefore, We suggest that Oriental medical approach may contribute to the management of Hypoxic Ischemic Encephalopathy.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.167-171
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• 2004

We constructed an oligo-d(T) primed directional cDNA library from the Bombyx mandarina whole larvae. In an effort to isolate genes expressed in the B. mandarina, 227 expressed sequence tags (ESTs) were generated by single-pass sequencing from the cDNA library. Sequence analysis showed that 107 clones (47.1%) were classified into known genes and 120 clones (52.9%) were novel transcripts, which are unknown for their function. Of the 107 known genes, the most abundant gene was found to be actin and followed by serine protease in the expression profile. Among these clones, a serine protease homolog (BmSP) which is a class of proteolytic enzymes isolated. Full-length sequence of the BmSP cDNA clone was 922 bp in length and has an open reading frame of 276 amino acids. The conserved histidine, aspatic acid and serine residues forming the catalytic center as well as cysteine residues contributing to three disulphide bonds also were found in Bmsp gene. mRNA expression analysis revealed a high and specific expression of the gene only in midgut tissue, suggesting that BmSP gene is closely associated with the expression of digestive enzyme.

• 한국산학기술학회논문지
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• 제20권1호
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• pp.162-169
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• 2019

본 연구는 65세 미만 성인과 65세 이상 노인에서 고혈압 위험요인을 파악하기 위한 서술적 조사연구이다. 6차 고령화 연구패널조사를 활용하여 6,152명의 연구대상자를 분석하였으며, 수집된 자료는 SPSS 23을 이용하여 카이검정과 로지스틱 회귀분석을 시행하였다. 고혈압 유병률에 영향을 미치는 위험요인에 대한 로지스틱 회귀분석 결과는 65세 미만 성인과 65세 이상 노인 둘 다에서, 주관적 건강상태, 당뇨병 유무, 심장질환 유무, BMI에 따른 비만정도가 위험요인으로 나타났고, 65세 미만에서는 만성폐질환, 소화기계 질환, 음주가, 65세 이상에서는 거주지역, 관절염 및 류마티스 질환, 흡연, 인지기능 정도가 고혈압에 영향을 미치는 위험요인으로 나타났다. 우리나라는 급속한 고령화 현상으로 인해 체계적이고 정책적인 만성질환관리가 절실하다. 특히, 나이에 따라 꾸준히 증가하는 고혈압에 대한 효과적인 관리를 위해 연령대에 따른 위험요인을 분석하는 것은 중요하다. 따라서 개인적 노후준비 뿐만 아니라 전반적인 사회복지수준 향상 및 노인복지대책을 통해 질병에 대한 관리가 필요하다.

• 구강생물연구
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• 제42권4호
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• pp.208-215
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• 2018

The purpose of this study was to identify the risk of dysphagia among patients that visited prosthodontics department, and evaluate the difference in risks arising from oral conditions and disease in order to preliminarily intervene the various influencing factors of dysphagia. A questionnaire was given to patients that were aged 65 years or older who visited the prosthodontics department between September to December 2017. The data was collected and analyzed using the t-test, $x^2-test$ and logistic regression analysis. The mean age of the patients was 75 years. Out of 300 patients, 206 patients (68.7%) had a risk of dysphagia. There were statistically significant differences between the -non-risk and risk groups, which included the number of natural teeth, total number of teeth including prosthesis, denture use, denture discomfort, number of tooth brushing, oral dryness, digestive system diseases, and musculoskeletal diseases. Among these, oral dryness was a risk factor while the total number of teeth, including natural teeth and prostheses, served as a protective factor. More than half of the elderly patients were at risk of dysphagia. Oral dryness is influenced by many factors and it should continuously be managed. Patients should fully recover their masticatory function by preserving the remaining teeth and compensating for the missing teeth. A dental practitioner should be fully aware of the risk of dysphagia in elderly patients and be able to intervene and offer proper patient health care in advance through treatment guidelines and education.