• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.283-283
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• 1996

The hydrolyzed-lactose milk for the lactase-deficient subject is sweeter than whole milk, and some subjects dislike its taste. To overcome this shortcoming the dried liposomes containing ${\beta}$-galactosidase to digest lactose in milk after drinking were prepared and examined the possible application of this dried liposomes to the lactase-deficient subjects. To improve the stability of conventional liposome suspension, the dried liposomes in the presence of trehalose were prepared by the dehydration-rehydration vesicles method. Small unilamellar vesicles, prepared with egg phosphatidyl cholesterol, and cholesterol, were mixed with ${\beta}$-galactosidase solution and then ;up[jo;ozed. The freeze-dried liposome was rehydrated and centrifuged. The resultant multilamellar vesicles were mixed with trehalose(4g/g lipid) and then lyophilized to produce final dried liposome. Trehalose increased the entrapping efficiency of liposomes by 3 fo1d compared to the liposomes without trehalose (13% vs. 46%).

• Journal of Plant Biology
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• v.28 no.3
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• pp.233-241
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• 1985

The isolation and culture of protoplasts from hypocotyl-derived calluses of Glycine max (L.) Merr. cv. Jangyeop were obtained by digestion for 6 hrs in an enzyme solution containing 3.5% cellulase, 1.5% macerozyme, 10% sorbitol and 0.1% CaCl2.2H2O at pH 5.8. Newly formed cell wall of protoplasts cultured in MS agar medium containing 10 $\mu$M $\alpha$-naphthaleneacetic acid (NAA) and 32 $\mu$M N6-benzylaminopurine (BAP) could be observed after 24 hrs culture. The first cell division of the protoplasts was observed after 3 days of culture; cell clusters after 2 weeks of culture. When transferred to solid media, the protoplasts formed cell clusters gave rise to proliferating calluses.

• Korean Journal of Veterinary Research
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• v.28 no.2
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• pp.387-390
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• 1988

A preliminary survey of Sarcocystis species in the slaughtered cattle in Seoul was undertaken during October through December 1987, using digestion diagnostic technique for the heart muscle specimens digested in 0.5% trypsin solution. Results indicated that 41.5% of 159 bovine hearts were infected with Sarcocystis proliferative forms. High frequencies in the exotic dairy cattle(42.2%) and the Korean native cattle(41.7%) were noticed in comparison with low frequency in the cross breed(25.0%). No differences were indicated between the sexes of the host animals, although an age difference was noticed as 48.9% in cattle older than four years in comparison with 39.0% and 37.1% in younger than two years and in two to four years, respectively.

• Journal of Microbiology and Biotechnology
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• v.5 no.4
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• pp.238-240
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• 1995

A novel and simple purification method for microbial $poly-{\beta}-hydroxybutyrate$ (PHS) was developed. Sodium hydroxide was found to be efficient for digesting cell materials. Initial biomass concentration, NaOH concentation, digestion time, and incubation temperature were optimized. When 40 g/l of biomass was incubated in 0.1 N NaOH at $30^{\circ}C$ for 1 h, PHB purity of 88.4% with a weight average molecular weight ($M_w$) of 770,000 and a polydispersity index (PI) of 2.4 was recovered with a yield of 90.8% from the biomass which initially contained PHB of a $M_w$ of 780,000 and a PI of 2.3.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.402-406
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• 2006

The physical and chemical characteristics of ALC were analyzed and showed 2.2 of specific gravity and 9.05 of pH. The results of leaching tests with standard method for soil and waste indicated heavy metals(Cu, Cd, Pb, $Cr^{6+}$) were under maximum concentration level. The anaerobic digestion sludge was attached in the surface of ALC within 90 hours. As the results of batch test, pH of the ALC and Bio-ALC were decreased from initial pH of ALC to 8.7 and 7.8 respectively Also, the concentration of heavy metals was rapidly eliminated in the solution with the batch test. The result of column experiment indicates that the removal efficiency of ALC was showed 66% of T-P, 60% of T-N, and 67% of CODcr. Also, removal efficiency of Bio-ALC was slightly higher than that of ALC in T-N (64%) and CODcr (74%).

• KSBB Journal
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• v.18 no.1
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• pp.35-38
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• 2003

Analysis of proteome in amniotic fluid was performed by 2-D PAGE (polyacrylamide gel electrophoresis). Proteins in amniotic fluid were separated by centrifugation and solubilized in buffer solution for IEF, using an IPG strip of pH 4-7L. Both a homogeneous slab gel of 12.5% and a gradient gel of 8-18%, were used. After 2-D PAGE, spots were stained with silver nitrate and picked up for in-gel digestion. Digested peptides were analyzed by MALDI-TOF and proteins were further identifical. More protein spots were detected in the gradient gels and a protein not previously reported was identified.

• Journal of the Korea Institute of Building Construction
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• v.9 no.3
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• pp.109-116
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• 2009

The magnesium sulfate solution digestion test carried out for resistance of concrete containing waste glass powder on magnesium sulfate attack. Moreover, it yielded S.D.F index was used for the criteria of quantitative assessment to the resistance of magnesium sulfate for the purpose of evaluation of chemical deterioration on concrete. Furthermore, to evaluate for micro-cracks within concrete and external corrosion, the weight variation of specimens and the dynamic elasticity were compared and analyzed and also the applicability was examined using the analysis of product of hydration through out observing external deformation and micro-structural deformation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.31-37
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• 1993

To analyze and classify the varietal variation of alkali digestibility in detail, which is closely connected with the gelatinization temperature and physical characteristics of cooked rice, the patterns of alkali decomposition changed along the alkali concentration were investigated for thirty three Korean leading rice cultivars and new breeding lines(japonica : 25, Tongil-type:8) including five glutinous rice. Principal component analysis was used to condense the information and to classify rice materials according to decomposed reaction pattern at several levels of potassium hydroxide(KOH) concentration. Thirty three rice varieties were classified largely into four groups by the distribution on the plane of upper two principal component scores which contained above 92% of total informations. Group I was consisted of one variety, Dobongbyeo, which owned almost same strong resistance to alkali digestion at the range of 0.8% to 1.6% KOH solutions. Group II included three japonica and Tongil-type glutinous rice varieties, which revealed medium alkali digestion value(ADV) at 1.4% KOH solution and intermediate change in ADV from 0.8% to 1.6% KOH solutions. Most of Tongil-type and early-maturity japonica rice, which exhibited medium-high ADV at 1.4% of KOH concentration and large ADV difference between low and high alkali solutions, were contained in Group III. Group N included most of medium or medium-late-maturity japonica, which showed high ADV at 1.4% KOH and medium or intermediate-high ADV change between low and high alkali solutions. The 1st principal component indicated the average index of ADV through 0.8-1.6% KOH solutions and the 2nd principal component pointed out the factor related with ADV difference between low and high alkali solutions or regression coefficients of ADV change along with the KOH concentrations.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1459-1465
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• 2013

Two in vitro experiments were conducted to evaluate the effect of methylcellulose (MC) on i) bacterial detachment from rice straw as well as ii) inhibition of bacterial attachment and fiber digestibility. To evaluate the effect of MC on fibrolytic bacterial detachment (Exp 1), in vitro bacterial cultures with 0.1% (w/v) MC solution were compared with cultures without MC after 8 h incubation. The effect of MC on inhibition of bacterial attachment was determined by comparing with real-time PCR the populations of F. succinogenes, R. flavefaciens and R. albus established on rice straw pre-treated with 0.1% MC with those on untreated straw after incubation for 0, 6 and 12 h (Exp 2). The major fibrolytic bacterial attachment on rice straw showed significantly lower populations with either the addition of MC to the culture or pre-treated rice straw compared to controls (p<0.05). Also, the digestibility of rice straw with MC was significantly lower compared with control (p<0.05). The F. succinogenes population did not show detachment from rice straw, but showed an inhibition of attachment and proliferation on rice straw in accordance with a decrease of fiber digestion. The detachments of Ruminococcus species co-existed preventing the proliferations with subsequent reduction of fiber degradation by MC during the incubation. Their detachments were induced from stable colonization as well as the initial adhesion on rice straw by MC in in vitro ruminal fermentation. Furthermore, the detachment of R. albus was more sensitive to MC than was R. flavefaciens. These results showed the certain evidence that attachment of major fibrolytic bacteria had an effect on fiber digestion in the rumen, and each of fibrolytic bacteria, F. succinogenes, R. flavefaciens and R. albus had a specific mechanism of attachment and detachment to fiber.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.632-635
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• 2003

For the disposal of low-level radwaste from nuclear power plant need the determination of levels of radio nuclides in radwaste. These nuclides include the difficult-to-measure nuclides, so indirect methodology for the determination of the difficult-to-measure nuclides have to be developed. In this work, for the determination of $^129I(t_{1/2}=1.57{\times}10^7 years)$ in low-level radwaste from nuclear power plant is investigated. Recovery of Iodide in simulated waste($UO_2$ pellet) as a soluble and radwaste(resin, woolen fabric)as a insoluble samples are measured. After pretreatment of sample, $I_2$ are extracted from aqueous solution with $CCl_4$. Then I are extracted from $CCl_4$ with 0.1M $NaHSO_3$ aqueous solution. iodide in aqueous solution are determined by ion chromatography. The overall recovery yield is 76.7 (RSD 1.7%) for mixed-acid digestion method. Incase of woolen fabrics, overall recovery yield is 74.3 (RSD 2.2%) and recovery of iodide in resin 56.5(RSD 5.6%) for alkaline fusion method.