• Title/Summary/Keyword: Digestible Amino Acid

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Effects of Crystalline Lysine, Threonine and Tryptophan Supplementation of Diets Containing Reduced Protein Levels on Performance of Growing Pigs

  • Li, D.F.;Xiao, C.T.;Kim, J.H.;Cho, W.T.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.1
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    • pp.43-50
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    • 1998
  • Three hundred and fifty-two growing pigs were utilized in three growth trials to investigate the effects of crystalline amino acid supplementation on growing pigs' growth performance and feed consumption. In Exp. 1, diets were formulated on the basis of digestible amino acids. Utilization of rapeseed meal plus cottonseed meal (R + C) in diet supported similar average daily gain (ADG), feed/gain (F/G) and cost per gain (p > 0.05) to group of soybean meal (SBM). In Exp. 2, 14% CP diets containing equal amount of cottonseed meal and rapeseed meal were formulated with the addition of threonine and/or tryptophan. Supplementation with threonine improved ADG and F/G (p < 0.05). Supplementation with only tryptophan made no benefits for ADG of growing pigs. In Exp. 3, diets with different threonine level were formulated. Increasing dietary threonine to 0.54% improved (p < 0.05) ADG and F/G compared to 0.45% dietary threonine. Increase in dietary threonine did not improve ADG (p > 0.05) but decreased F/G (p < 0.05) because of a decrease in average daily feed intake. Pigs fed diet with dietary threonine/lysine ratio of 67.5% supported optimum growth performance.

Current Trends of Research and Developments of Infant Formula in Korea (국내산 조제분유의 최근 연구 개발 동향)

  • Jeon, Jeong-Wook;Lee, Keon-Won;Kim, Yong-Ki;Yun, Sung-Seob
    • Journal of Dairy Science and Biotechnology
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    • v.26 no.2
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    • pp.1-8
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    • 2008
  • Human milk contains the nutrients for the growth and the development of the newborn. It contains specific proteins, lipids, and other components designed to be easily digestible and which play important roles in infant nutrition. However, when the lactation is failure or milk secretion is not enough, bovine milk based dried formulations, which is called infant formula, have been widely used for infant feeding. In this study, we investigated protein composition and amino acids profile related to easy digestion; fatty acids composition and LCPUFA(DHA and ARA) related to brain and eye development; synbiotics which take advantage of both probiotics and prebiotics; and colostrum-originated functional micronutrient as major issues in the recent infant formula. Domestic infant formulas, which are based on the research of human and bovine milk rich in functional components for immunity, growth, anti-allergenicity, digestion and brain development, have been developed to be similar to human milk.

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Effects of Origins of Soybean Meal on Growth Performance, Nutrient Retention and Excreta Microflora of Broilers

  • Ku, Yun;Ingale, Santosh Laxman;Kim, Jin Soo;Kim, Kwang Hyun;Lee, Su Hyup;Chae, Byung Jo
    • Korean Journal of Poultry Science
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    • v.40 no.2
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    • pp.129-138
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    • 2013
  • An experiment was conducted to determine the effects of origins of soybean meal (SBM) on growth performance, nutrients and energy retention and fecal microflora in broilers. The SBM originating from Korea, Brazil and India were used. A total of 480 broiler chicks (average initial BW, 41.8 g) were randomly allotted to 6 treatments. Each treatment had 4 replicate pens with 20 chicks per pen. Birds were fed diets containing SBM originated from Korea (domestic SBM), Brazil or India (imported SBM) and the diets were based on the total amino acid (TAA) or true digestible amino acid (TDAA). Experimental diets were fed in two phases, starter (d 0~21) and finisher (d 22~35). The overall weight gain, feed intake and FCR were better (P<0.05) in birds fed Korean SBM. During starter and finisher periods, birds fed diets containing Korean SBM had greater (P<0.05) retention of GE, DM, and CP (P<0.05) as compared with Birds fed diets containing SBM from India. In addition, diets formulated on TDAA basis had grater (P<0.05) CP retention during starter period and DM, GE and CP retention during finisher period. There were no differences (P>0.05) in the excreta microflora and overall feed cost per kg body weight gain in birds fed SBM from different origins; however, diets formulated on TDAA basis had lesser (P<0.05) feed cost per kg body weight gain in birds when compared with diets formulated on TAA basis. These results indicate that Korean SBM has better nutrients digestibility than SMB originated from Brazil and India, which contributes to the improved performance of broilers. In addition, better performance was obtained when diets were formulated on TIDAA basis.

Effects of adaptation time and inclusion level of sugar beet pulp on nutrient digestibility and evaluation of ileal amino acid digestibility in pigs

  • Zhang, Ze Yu;Zhang, Shuai;Lai, Chang Hua;Zhao, Jin Biao;Zang, Jian Jun;Huang, Cheng Fei
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.9
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    • pp.1414-1422
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    • 2019
  • Objective: Two experiments were conducted to determine the effects of adaptation time and inclusion level of sugar beet pulp (SBP) on nutrient digestibility and to evaluate the ileal amino acid digestibility of SBP fed to pigs. Methods: In Exp. 1, thirty-six crossbred barrows ($85.0{\pm}2.1kg$) were allotted to 6 diets in a completely randomized design with six replicates per diet. Diets included a corn-soybean meal diet and 5 test diets containing 14.6%, 24.4%, 34.2%, 43.9%, or 53.7% SBP, respectively. The adaptation time consisted 7, 14, 21, or 28 d consecutively for each pig followed by 5 d for fecal collection. Feces were collected from d 8 to 13, d 15 to 20, d 22 to 27, and d 29 to 34, respectively. In Exp. 2, six pigs ($35.1{\pm}1.7kg$) with T-cannulas at the terminal ileum were fed to 3 diets in a replicated $3{\times}3$ Latin square design with 3 periods and 2 replicate pigs per diet. Each period consisted 5 d for diet adaptation followed by 2 d for digesta collection. Results: The digestible energy (DE) value and the apparent total tract digestibility (ATTD) of gross energy (GE), dry matter (DM), ash, and organic matter in diets linearly decreased (p<0.05) as the adaptation time increased or as the dietary SBP increased, while the ATTD of neutral detergent fibre and acid detergent fibre in diets linearly increased (p<0.01) as the dietary SBP increased. The DE value and the ATTD of GE and crude protein (CP) in SBP linearly increased (p<0.05) as the adaptation time increased, while the ATTD of CP in SBP linearly decreased (p<0.01) as the inclusion level increased. The standardized ileal digestibility of Lys, Met, Thr, and Trp in SBP was 37.03%, 51.62%, 40.68%, and 46.22%, respectively. Conclusion: The results of this study indicated that the ATTD of energy and nutrients were decreased as inclusion rate of SBP increased.

Feeding and Management System to Reduce Environmental Pollution in Swine Production - Review -

  • Han, In K.;Lee, J.H.;Piao, X.S.;Li, Defa
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.3
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    • pp.432-444
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    • 2001
  • In this manuscript, several effective feeding and management systems to reduce environmental pollution in swine production have been briefly introduced. It is logical that reducing the excretion of nutrients in manure should be the first step to reduce the environmental impact of pig production. it is evident that the excretion of nitrogen and phosphorus can be reduced when more digestible or available feedstuffs are used. Also, it is well known that proper feed processing can reduce anti nutritional factors (ANF) and improve nutrient digestibilities. Supplementation of effective feed additives can reduce excretion of nitrogen and phosphorus due to efficient feed utilization. These include enzymes (e.g., phytase), antibiotics, probiotics, organic acids and growth hormones ($\beta$-agonists and porcine somatotropin). One of the most effective ways to reduce pollutants from swine manure is to use synthetic amino acids in feed manufacturing. Many studies showed that reduction of 2 to 4% unit (U) of dietary protein with supplemental amino acid (AA) could dramatically reduce (15 to 20%) nitrogen excretion. Regarding feeding strategies, it has been recognized that phase feeding regimen could be used to reduce nitrogen and phosphorous excretion by feeding pigs in better agreement with age and physiological state. Feeding barrows and gilts separately, known as split sex feeding, can also decrease excretion of nitrogen and phosphorus. With the increasing concerns on the negative impact of animal production systems on the environment, animal nutritionists and producers should be aware that sustainability of animal agriculture is as important as high production performance. Therefore, some feeding and management strategies described in this manuscript will help to reduce environmental pollution in swine production. Proper combination of feeding regimen and environment-friendly diet formulation through nutritional approach will be more effective to reduce nutrient excretion in swine production system compared to single approach to do so.

NIR PREDICTIONS OF INDIRECT RESPONSES

  • Claridge, Rovert-Burling;Ross Clarke
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.3103-3103
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    • 2001
  • NIR has been extensively used to predict directly measurable properties of materials that are important to the appropriate industries. Commonly, NIR is used to perform fast, routine tests to improve control response as against the response time for the (normally chemical) base test. This paper discusses the use of NIR to measure indirect properties of materials. In these cases, the pure chemical or physical tests are either unable measure the appropriate parameters (eg GMO modification) or there are mitigating effects that are not properly addressed by the base tests. In particular, we looked at the digestible portion of amino acids within meat and bone meal. This is the desired response measurement by end-users of the product (intensive livestock producers) but is currently unable to be offered as a measurement by producers. The base test method is by controlled feeding trials. These are somewhat cumbersome, taking 2-3 months, involving several sets of animals, and considerable expense. A shortened test (feed trial based) would be of little use, as the precision blows out over short period feeding trials. For example, a rat ileal digestibility test requires around 2 months, and costs some $USD1000. This is clearly impractical test for a producer involved in continuous production, with a 1-2 day turn around. While the amino acid abundance is accessible chemically, the uptake of amino acids into usable material by mammalian species is not simply related to the measured abundance within the material. There are many co-related material properties that might help or hinder uptake, some chemical based (eg protein damage), some indirect (eg palatability), some physiological (intestinal tract response vs speed of throughput). We discuss the approaches taken to provide a suitable reference data set, and present the derived prediction and validation relationships.

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Effects of Supplemental Liquid DL-methionine Hydroxy Analog Free Acid in Diet on Growth Performance and Gastrointestinal Functions of Piglets

  • Kaewtapee, C.;Krutthai, N.;Bunchasak, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.8
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    • pp.1166-1172
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    • 2016
  • This study was conducted to determine the effect of dietary supplementation of liquid DL-methionine hydroxy analog free acid (DL-MHA) on growth performance and gastrointestinal conditions of piglets. One hundred and eighty crossbred barrow piglets (Large White${\times}$Landrace, body weight: $12.48{\pm}0.33kg$) were divided into three groups with ten replications of six piglets each. Piglets received DL-MHA in diet at a concentration of 0 (control group), 0.15%, or 0.24%. The results indicated that increasing the standardized ileal digestible (SID) of sulfur amino acids (SAA) to lysine (SID SAA:Lys) ratio by supplementation of DL-MHA tended to increase (quadratic; p<0.10) weight gain and ADG, and showed slightly greater (linear; p<0.10) gain:feed ratio. The pH in the diet and cecum linearly decreased (p<0.01), whereas pH in colon had a quadratic response (p<0.01) with increasing supplementation of DL-MHA. By greater supplementation of DL-MHA, the population of Lactobacillus spp. in rectum was likely to increase (quadratic; p<0.10), but Escherichia coli population in the diet was reduced (quadratic; p<0.05). Acetic acid concentration and total short-chain fatty acids in cecum linearly increased (p<0.05), whereas valeric acid in cecum quadratically increased (p<0.05) with increasing DL-MHA levels. Moreover, the villous height of the jejunum quadratically increased (p<0.01) as the supplementation of DL-MHA was increased. It is concluded that the addition of DL-MHA in diet improved the growth performance and the morphology of gastrointestinal tract of piglets.

Effects of inclusion level and amino acid supplementation on energy values of soybean oil determined with difference or regression methods in growing pigs

  • Wang, Qiuyun;Huang, Chengfei;Liu, Mei;Liu, Ling;Zhang, Shuai
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.11
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    • pp.1809-1816
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    • 2020
  • Objective: This study was conducted to evaluate the effects of inclusion level and amino acid (AA) supplementation on energy values of soybean oil (SO) as determined by difference method or regression method when fed to growing pigs. Methods: Thirty-six barrows (initial body weight: 28.0±1.3 kg) were randomly assigned to one of 6 dietary treatments, which included 2 control diets formulated using a basal diet with or without AA supplementation, and 4 experimental diets with 5% or 10% SO addition in the 2 control diets, respectively. All pigs were individually housed in metabolism crates for 19 d, and during the last 5 d, total urine and feces production were collected. The nutrient digestibility in diets and the digestible energy (DE) and metabolizable energy (ME) values of SO were determined using the difference method and the regression method, respectively. Results: Our results showed that there were no interaction effects (p>0.05) between AA supplementation and SO inclusion levels on energy values of SO and dietary nutrient digestibility. The DE and ME values of SO determined by the difference method were not affected (p>0.05) by AA supplementation, however, the ME value of SO increased (p<0.05) as the inclusion level of SO increased. Moreover, the energy values of SO determined using the regression method were close to those determined using difference method with 10% SO inclusion, but were greater than those obtained using difference method with 5% SO inclusion. Conclusion: We concluded that the DE and ME values of SO increased with the inclusion level but were not affected by AA supplementation in the range of 0% to 10%. The difference method can substitute for the regression method to determine the DE and ME values of SO when the inclusion level is 10%, but not at 5% inclusion level.

Fibrobacter succinogenes, a Dominant Fibrolytic Ruminal Bacterium: Transition to the Post Genomic Era

  • Jun, H.S.;Qi, M.;Ha, J.K.;Forsberg, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.5
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    • pp.802-810
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    • 2007
  • Fibrobacter succinogenes, a Gram-negative, anaerobic ruminal bacterium is a major fibre digesting species in the rumen. It intensively degrades plant cell walls by an erosion type of mechanism, burrowing its way through the complex matrix of cellulose and hemicellulose with the release of digestible and undigested cell wall fragments. The enzymes involved in this process include a combination of glucanases, xylanases, arabinofuranosidase(s) and esterases. The genome of the bacterium has been sequenced and this has revealed in excess of 100 putative glycosyl hydrolase, pectate lyase and carbohydrate esterase genes, which is greater than the numbers reported present in other major cellulolytic organisms for which genomes have been sequenced. Modelling of the amino acid sequences of two glycanases, CedA and EGB, by reference to crystallized homologs has enabled prediction of the major features of their tertiary structures. Two dimensional gel electrophoresis in conjunction with mass spectroscopy has permitted the documentation of proteins over expressed in F. succinogenes grown on cellulose, and analysis of the cell surfaces of mutant strains unable to bind to cellulose has enabled the identification of candidate proteins with roles in adhesion to the plant cell wall substrate, the precursor to cellulose biodegradation.

Growth Performance, Body Composition and Protein and Energy Utilization of Pigs Fed Ad Libitum Diets Formulated According to Digestible Amino Acid Content

  • Raj, St.;Fandrejewski, H.;Weremko, D.;Skiba, G.;Buraczewska, L.;Zebrowska, T.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.6
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    • pp.817-823
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    • 2000
  • Four groups of six growing gilts each were fed ad libitum diets composed of cereals and soyabean (SBM) or rapeseed (RSM) meal and containing two levels of crude protein: high - 18.0% (RSM-H) and 16.9% (SBM-H) or low - 15.6% (RSM-L) and 15.1% (SBM-L). The diets were balanced by supplementation with crystalline amino acids and contained apparent ileal digestible lysine, methionine, threonine and tryptophan in proportions (1.00:0.32:0.57:0.18) according to CVB (1995). Voluntary feed intake, weight gain and slaughter and chemical body composition of animals were assessed. Protein and energy balances from 25 to 70 kg body weight were calculated by the comparative slaughter method. Protein source had a significant effect on voluntary feed intake; it was 0.12 kg/d lower in pigs fed the SBM than RSM-diets. Pigs fed on the SBM-L diet consumed the least amount of feed (2.17 kg). Daily gain (average, $900{\pm}12.59g$) and feed conversion ratio ($2.54{\pm}0.04kg/kg$) were not statistically affected by source (SMB and RSM) and protein level (high and low). In empty body similar amounts (g/kg) of protein (163 g), water (635 g) and ash (28 g) were found. However, pigs fed the RSM-L diet were fatter than those fed the SBM-L diet (188 vs. 161 g/kg). No statistical differences were observed in daily protein deposition, which on average amounted to $142{\pm}11g$, or carcass characteristics. An improvement of crude protein utilization by 6.3 percentage units was found by decreasing the protein concentration in the diets. Heat production in the body was not significantly affected by the treatments.