• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.131-131
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• 2017

Though the Platycodon grandiflorum, has a broad range of pharmacologic properties, but the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two-dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}2-fold$) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, the frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). Taken together, the protein profile may provide insight clues for better understanding the characteristics of proteins and its metabolic activities in various explants of this essential medicinal plant P. grandiflorum.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.97-106
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• 2015

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

• Journal of the Korean Institute of Telematics and Electronics
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• v.9 no.4
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• pp.33-37
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• 1972

Amplifier circuits with differential characteristics, that is, amplifier circuits the voltage gain of which are proportional to the complex frequency are described. It is shown that the characteristics of the circuit predicted on the basis of the nullator-norator model of the transistor coincides with the result of the exact analysis of the circuit, and experimental result coincides with the theory.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2013.05a
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• pp.389-390
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• 2013

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.7
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• pp.1069-1072
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• 2002

고도로 발달된 정보화 시대에서 우리가 원하는 정보를 짧은 시간, 적은 비용으로 서로 주고 받기 위해서는 이것에 맞는 시스템이 요구된다. 반도체 chip의 대용량과 고속화됨으로써 TTL, ,LVTTL등이 data 100Mbps 정도를 안전하게 전달 할 수 있는 능력이 있으므로 그 이상을 전달할 수 있는 새로운 Logic level이 필요하게 되었다. 이에 맞추어 신호 level의 여러 가지 중 본 논문에서는 Virtex II XC2V 1000 FF896을 이용하여 Differential I/O LVDS( Low Voltage Differential Signaling ) level 특성을 clock, Data와의 전송관계를 Eye_Pattern을 통해 살펴보았다.

• Proceedings of the Korean Institute of Communication Sciences Conference
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• 2006.07a
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• pp.325-325
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• 2006

• Journal of the Korean Society for Railway
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• v.3 no.4
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• pp.213-218
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• 2000

Waste landfill is so loose that it may cause the insufficient bearing capacity and the differential settlement. And so, characteristics and conditions of the ground should be considered in applications of ground improvement in waste landfill. In this paper, analysis of field tests as the static loading test and the bearing capacity test were performed. In result, PG(Pack Grouting) pile method is proved effective and economic, because it could bring about the increase of end bearing capacity, the prevention of differential settlement and increase of density by expansion of pile.

• 유체기계공업학회:학술대회논문집
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• 2001.11a
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• pp.413-418
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• 2001

Emergency shut-on valve was developed to shut off natural gas at the front of a gas meter in the house. The shut-off flow rate and differential pressure of this valve was controlled by adjusting the distance between the spool and magnet. Also the spool shape was an important factor in the performance of this valve. The experimental and computational results will be useful for the design having better performance.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.61.6-61
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• 2002

In these days, Block Pulse functions are used in a variety of fields such as the analysis and controller design of the systems. In applying the Block Pulse function technique to control and systems science, the integral operation of the Block Pulse series plays important roles. This is because differential equations are always involved in the representations of continuous-time models of dynamic systems, and differential operations are always approximated by the corresponding Block Pulse series through integration operational matrices. In order to apply the Block Pulse function technique to the problems of continuous-time dynamic systems more efficiently, it is necessary to find th...

• 제어로봇시스템학회:학술대회논문집
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• 1995.10a
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• pp.227-230
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• 1995

Stability of a coupled nonautonomous ordinary differential equation is investigated. Asymptotic convergence to zero of a part of state vector is additionally shown, otherwise only uniform stability could have been concluded by the Lyapunov direct method. Obtained results could be particularly useful in analysis of nonautonomous systems in which the invariance principle does not hold. An illustrating example is given.