• BMB Reports
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• 제37권1호
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• pp.1-10
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• 2004

DNA and RNA quantifications are widely used in biological and biomedical research. In the last ten years, many technologies have been developed to enable automated and high-throughput analyses. In this review, we first give a brief overview of how DNA and RNA quantifications are carried out. Then, five technologies (microarrays, SAGE, differential display, real time PCR and real competitive PCR) are introduced, with an emphasis on how these technologies can be applied and what their limitations are. The technologies are also evaluated in terms of a few key aspects of nucleic acids quantification such as accuracy, sensitivity, specificity, cost and throughput.

• Structural Engineering and Mechanics
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• 제13권3호
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• pp.329-343
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• 2002

In this research, the dynamic stability of an orthotropic elastic conical shell, with elasticity moduli and density varying in the thickness direction, subject to a uniform external pressure which is a power function of time, has been studied. After giving the fundamental relations, the dynamic stability and compatibility equations of a nonhomogeneous elastic orthotropic conical shell, subject to a uniform external pressure, have been derived. Applying Galerkin's method, these equations have been transformed to a pair of time dependent differential equations with variable coefficients. These differential equations are solved using the method given by Sachenkov and Baktieva (1978). Thus, general formulas have been obtained for the dynamic and static critical external pressures and the pertinent wave numbers, critical time, critical pressure impulse and dynamic factor. Finally, carrying out some computations, the effects of the nonhomogeneity, the loading speed, the variation of the semi-vertex angle and the power of time in the external pressure expression on the critical parameters have been studied.

• 전력전자학회:학술대회논문집
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• 전력전자학회 1999년도 전력전자학술대회 논문집
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• pp.218-221
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• 1999

We introduce the systematization design method using similarity theory which is profitable in the compatability, the reduction of construction time and price. The design method can make us predict the characteristic experiment for the magnitude we desire as the expression equation applied continuously. We can induce the design sample the user demand with the verification of the data on optimum design previously. Therefore, in case of designing and developing the products, systematization design method is very useful for the standardization of the developed goods, compatability, the reduction of construction time and price. In this paper, we presented the analogical algorithms of systematization design using similarity theory, design factors and processing method of the restriction factors. Also, we analyzed the output voltage in terms of input voltage and displacement as choosing a differential transformer as the model.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제49권9호
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• pp.578-583
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• 2000

We introduce the systematization design method using analogical analysis. The design method can make us predict the characteristic experiment for the magnitude we desire as the expression equation applied continuously. We can induce the design sample the users demand with the verification of the data on optimum design previously. Therefore in case of designing and developing the products systematization design method is very useful for the standardization of the developed goods compatability the reduction of construction time and price. In this paper we present the analogical algorithms of systematization design using similarity theory design factors and processing method of the restriction factors. Also we analyze the output voltage in terms of input voltage and displacement as choosing a differential transformer as the model.

• Kyungpook Mathematical Journal
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• 제53권3호
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• pp.459-465
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• 2013

Let $p(z)$ be an analytic function defined on the open unit disk D and $p(0)=1$. Condition ${\beta}$ in terms of complex numbers D and real E with -1 < E < 1 and ${\mid}D{\mid}{\leq}1$ is determined such that $1+{\beta}{\prec}\frac{1+Dz}{1+Ez}$ implies $p(z){\prec}\sqrt{1+z}$. Furthermore, the expression $1+\frac{{\beta}zp^{\prime}(z)}{p(z)}$ and $1+\frac{{\beta}zp^{\prime}(z)}{p^2(z)}$ are considered in obtaining similar results.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권6호
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• pp.362-366
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• 2002

A mathematical model has been developed for describing the oxygen concentration during the exponential growth of microorganisms, in a static solid substrate bed supported on a tray fermentor. The model equations comprise of one partial differential equation for mass transfer and an ordinary differential equation of growth. After nondimensionlisation, analytical solution tn the model has been obtained by the method of Laplace transforms. An expression for critical thickness of bed is deduced from the model equation. The significance of the model in the design of tray fermentors is discussed. The validity of the discussion is verified by taking an illustration from the literature.

• 대한수학회지
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• 제61권4호
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• pp.743-760
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• 2024

In this paper, we are devoted to study a forth order curve flow for a smooth closed curve in centro-affine geometry. Firstly, a new evolutionary equation about this curve flow is proposed. Then the related geometric quantities and some meaningful conclusions are obtained through the equation. Next, we obtain finite order differential inequalities for energy by applying interpolation inequalities, Cauchy-Schwartz inequalities, etc. After using a completely new symbolic expression, the n-order differential inequality for energy is considered. Finally, by the means of energy estimation, we prove that the forth order curve flow has a smooth solution all the time for any closed smooth initial curve.

• IMMUNE NETWORK
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• 제1권1호
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• pp.53-60
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• 2001

Background: The human mic2 gene is a pseudoautosomal gene that encodes a cell surface antigen, CD99. High levels of CD99 constitute a tumor marker in Ewing s sarcoma (ES). We have recently demonstrated that CD99-induced apoptosis occurs only in undifferentiated ES cells, not in differentiated ES cells, raising the possibility of the involvement of CD99 in neural ontogeny. Methods: To elucidate the relations between the expression of CD99 and the differentiation of neural cells and the mechanism by which the expression of CD99 is regulated, we analyzed the differential patterns of CD99 expression in SH-SY5Y by treatment of 12-O-tetradecanoyl-13-phorbol acetate (TPA) and retinoic acid. In addition, to explore the transcriptional activity of CD 99 during neural cell differentiation, SH-SY5Y cells were transiently transfected with a CD99 promoter-driven luciferase construct, and treated with the inducers. Results: In immunoblotting and flow cytometry, the expression level of CD99 was increased on differentiated SH-SY5Y cells induced by TPA and retinoic acid. The luciferase activity was elevated by the treatment with TPA, known to mature SH-SY5Y cells toward a sympathetic neuronal lineage, whereas retinoic acid inducing a sympathetic chromaffin lineage displayed little effect. Conclusion: The result indicates that CD99 might be expressed only on cells maturing toward a neuronal lineage among differentiating primitive neuronal cells. In addition, the expression of CD99 seems to be regulated at the transcriptional level during the differentiation.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5725-5728
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• 2012

Growing evidence shows that deregulation of the circadian clock plays an important role in the development of malignant tumors, including gliomas. However, the molecular mechanisms of gene chnages controlling circadian rhythm in glioma cells have not been explored. Using real time polymerase chain reaction and immunohistochemistry techniques, we examined the expression of two important clock genes, cry1 and cry2, in 69 gliomas. In this study, out of 69 gliomas, 38 were cry1-positive, and 51 were cry2-positive. The expression levels of cry1 and cry2 in glioma cells were significantly different from the surrounding non-glioma cells (P<0.01). The difference in the expression rate of cry1 and cry 2 in high-grade (grade III and IV) and low-grade (grade 1 and II) gliomas was non-significant (P>0.05) but there was a difference in the intensity of immunoactivity for cry 2 between high-grade gliomas and low-grade gliomas (r=-0.384, P=0.021). In this study, we found that the expression of cry1 and cry2 in glioma cells was much lower than in the surrounding non-glioma cells. Therefore, we suggest that disturbances in cry1 and cry2 expression may result in the disruption of the control of normal circadian rhythm, thus benefiting the survival of glioma cells. Differential expression of circadian clock genes in glioma and non-glioma cells may provide a molecular basis for the chemotherapy of gliomas.

• BMB Reports
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• 제44권8호
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• pp.535-540
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• 2011

Reprogramming errors, which appear frequently in cloned animals, are reflected by aberrant gene expression. We previously reported the aberrant expression of TIMP-2 and PBEF in cloned placenta and differential expression of PBEF genes during pregnancy. To examine the epigenetic modifications that regulate dynamic gene expression in developing placentae, we herein analyzed the mRNA and protein expression levels of PBEF and TIMP-2 in the placentae of normal mice during pregnancy and then examined potential correlations with epigenetic modifications. DNA methylation pattern analysis revealed no difference, but ChIP assays using antibodies against H3-K9/K14 and H4-K5 histone acetylation revealed that the H3-K9/K14 acetylation levels, but not the H4-K5 acetylation levels, of the TIMP-2 and PBEF loci were significantly correlated with their gene expression levels during placentation in normal mice. These results suggest that epigenetic changes may regulate gene expression level in the developing placentae of normal mice and that inappropriate epigenetic reprogramming might be one cause of the abnormal placentae seen in cloned animals.