• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
• /
• pp.176.2-176
• /
• 1999

No Abstract, See Full Text

• 전자공학회논문지
• /
• 제51권11호
• /
• pp.94-100
• /
• 2014

본 논문에서는 전류-재사용 구조를 사용하여 1.95 GHz~3.15 GHz 의 광범위한 튜닝 범위를 갖는 저전력 전압 제어 발진기(VCO)를 설계하였다. 클래스-C 타입을 적용하여 위상 잡음 특성을 향상 시켰으며, 2 단계 자동 진폭 캘리브레이션 기법을 통해 전류-재사용 전압제어발진기 구조의 가장 큰 단점인 차동 출력 전압간의 불균형을 최소화 하였다. 차동 출력 전압간의 차이는 1.5mV ~ 4.5mV 가량으로 나타나며, 이는 출력 전압의 0.6% 이내 오차이다. 제안하는 전류-재사용 전압제어발진기는 CMOS $0.13{\mu}m$ 공정을 사용하여 설계 하였다. 공급 전압은 1.2 V를 사용하였고, 소모 전류는 2.3 GHz에서 2.6 mA이다. 출력주파수가 2.3 GHz에서 위상 잡음은 -116.267 dBc/Hz(@1MHz Offset)이며, 레이아웃 면적은 $720{\times}580{\mu}m^2$ 이다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권9호
• /
• pp.1192-1196
• /
• 2004

In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences in gene expression of pig's Longissimus dorsi between the high-parent heterosis cross combination Landrace${\times}$Large White and the mid-parent heterosis cross combination Large White${\times}$Meishan. Three pig purebreds, Large White, Meishan, and Landrace and four types of reciprocal $F_1$ hybrids were analyzed using nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers and nearly 7,000 reproducible bands were examined. The patterns of gene expression of each cross combination were analyzed and eight common patterns (fifteen kinds) were found. When the results from the two cross combinations were put together and compared, eight different typical expression patterns were observed, these indicated that the patterns of gene expression of these two cross combinations had obvious differences. Gene expression correlation and cluster analyses of the two cross combinations indicated that the gene expression of the mid-parent heterosis cross combination was correlated with maternal effect, but in the high-parent heterosis cross combination, paternal effect acted in the gene expression of the hybrids or the gene expression of the hybrids was biased towards one parent.

• Plant Biotechnology Reports
• /
• 제4권2호
• /
• pp.165-172
• /
• 2010

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1.5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of ${\beta}$-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제13권1호
• /
• pp.31-35
• /
• 2006

The ubiquitin conjugating enzyme 2 (E2) is core component of ubiquitin proteasome pathway (UPP) which represents a selective mechanism for intracellular proteolysis in eukaryotic cells. The E2 has been implicated in the intracellular transfer of ubiquitin to target protein. We show here the involvement of E2 in antiviral immune of Bombyx mori to Bombyx mori nuclear polyhedrosis virus (BmNPV). In this study, mRNA fluorescent differential display PCR (FDD-PCR) was performed with BmNPV highly resistant silkworm strain NB and susceptible silkworm strain 306. At 24 h post BmNPV infection, FDD-PCR with the arbitrary primer AP34 showed that one cDNA band was down-regulated in the midgut of resistant strain, but highly expressed in susceptible strain. The deduced amino acid sequence of this cDNA clone share 99% identity with the recently published B. mori ubiquitin conjugating enzyme E2 (Genbank NO: DQ311351). Fluorescent quantitative PCR corroborated down regulation of E2 in resistant strain. We there conclude that BmNPV infection evokes strong response of susceptible strain including activation of UPP. BmNPV may evolve escape mechanisms that manipulate the UPP in order to persist in the infected host. In addition, the identification of down-regulation of E2 in resistant strain, as well as structure data, are essential to understanding how UPP operates in silkworm antiviral immune to BmNPV disease.

• BMB Reports
• /
• 제33권2호
• /
• pp.184-187
• /
• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• 한국발생생물학회지:발생과생식
• /
• 제9권1호
• /
• pp.43-48
• /
• 2005

조혈세포의 주요 서식지가 되는 골수기질세포는 줄기세포의 운영을 결정하는 다양한 인자들을 제공한다. 방사선 요법은 항암치료법으로 널리 활용되고 있으나, 조혈세포의 파괴로 인한 부작용이 심각한 문제로서 조혈세포에 의한 혈액 세포가 빠른 시간 내에 회복되는 것이 필수적이다. 본 연구에서는 방사선을 조사했을 때의 줄기세포 서식지를 구성하는 세포인 골수기질세포에서 발현되는 유전자를 탐색하여 그 기능과 조절 및 혈액 형성을 이해하는 기초를 마련하고자 하였다. 방법론적으로는 polymerase chain reaction(PCR) 및 agarose 전기영동 방법을 활용한 differential display를 활용하였으며, 결과로서 여러 후보 유전자가 선별되었으나, plasminogen activator inhibitor-1(PAI-1) 유전자만이 감마선에 의해 유도됨이 반복 확인되었다. PAI-1 유전자 유도의 의미는 향후에 더 연구해야 할 것이다.

• 한국통신학회논문지
• /
• 제34권4B호
• /
• pp.349-359
• /
• 2009

본 논문에서는 3차원 효과의 최대화를 위한 스테레오 비디오 전송 방법을 제안한다. 기존의 다중스트림 동기화 기술은 단일 비디오와 오디오간의 지연을 최소화 하는 것으로, 둘 이상의 비디오가 동시에 필요한 3차원 비디오스트림 동기화에는 적합하지 않다. 본 논문에서는 인간에게 자연스럽고, 입체적으로 느껴지는 3차원 영상을 만들어 낼 수 있는 시간 차이의 허용 범위를 고려하여 다중 영상을 합성하는 기준을 제시하였고, 이를 위한 비디오 스트림 간의 동기화 방법을 제안하였다. 본 연구에서는 주관적 화질 평가를 통하여 스테레오 비디오를 3차원으로 느낄 수 있는 시간 차이의 허용범위를 측정하였다. 스테레오 비디오의 동기화를 위해, 전송된 각 비디오 스트림들의 취득 시간을 구하고, 시간 차이 허용 범위 안에 포함된 비디오 스트립을 이용하여 3차원 영상으로 합성한 뒤, 디스플레이 장치를 통해 재생한다. 제안한 기술의 성능을 평가하기 위해 실시간 다중 비디오 통신 시스템을 구현하였고, 주관적 화질 평가를 통해 제안한 동기화 제어 기술의 성능을 평가하였다. MOS (Mean Opinion Score) 측정 결과, 제안하는 기술을 통하여 3차원 디스플레이 장치에 재생한 영상은 DMOS (Differential Mean Opinion Score) 실험 스케일 중 매우 좋음과 좋음 범위에 속하는 것을 확인하였다.

• The Plant Pathology Journal
• /
• 제19권5호
• /
• pp.239-247
• /
• 2003

In Korea, a local soybean (Glycine max) genotype 56l. was found to be strongly resistant to a virulent bacterial strain of a Pseudomonas sp. SN239. Specific genes involved in the resistance of the soybean genotype 561 were identified and the pattern of gene expression against the Pseudomonas infection was analyzed using differential-display reverse transcription PCR (DDRT-PCR). More than 126 cDNA fragments representing mRNAs were induced within 48 hours of bacteria inoculation. Among them, 28 cDNA fragments were cloned and sequenced. Twelve differentially displayed clones with open reading frames had unknown functions. Sixteen selected cDNA clones were homologous to known genes of other organisms. Some of the identified cDNAs were pathogenesis-related (PR) genes and PR-like genes. These cDNAs included a putative calmodulin-binding protein; an endo-l,3-1,4-$\bate$-D-glucanase; a $\bate$-1,3-endoglucanase; a $\bate$-1,3-exoglucanase; a phytochelatin synthetase-like gene; a thiol protease; a cycloartenol synthase; and a putative receptor-like serine/threonine protein kinase. Among them, four genes were found to be putative PR genes induced significantly by the Pseudomonas infection. These included a calmodulin-binding protein gene, a $\bate$-1,3-endoglucanase gene, a receptor-like serine/threonine protein kinase gene, and pS321 (unknown function). These results suggest that the differentially expressed genes may mediate the strong resistance of soybean 561 to the strain SN239 of Pseudomonas sp.

• 한국초지조사료학회지
• /
• 제27권4호
• /
• pp.241-248
• /
• 2007

Cytokinin은 식물의 성장과 발달에 중요한 역할을 하는 필수 호르몬이다. mRNA differential display 방법으로 애기장대 amp1 돌연변이체로부터 cytokinin에 의하여 발현이 유도되는 PR4 유전자를 분리하였다. AtPR4로 명명한 애기장대 PR4 유전자는 212개의 아미노산으로 구성되어 있었으며 분자량은 22,900이고 등전점은 7.89로 추정되었다. Genomic DNA 분석결과, AtPR4는 single copy 유전자인 것으로 나타났다. AtPR4의 mRNA는 cytokinin과 NaCl에 의해서는 발현이 유도되었지만 SA와 JA에 의해서는 발현이 억제되었다. PR 단백질은 내병성 등 생체방어기작에 관여하는 것으로 알려져 있다. 따라서 본 연구에서 분리한 애기장대 PR4 유전자는 내병성 목초 품종의 개발에 유용하게 사용될 것으로 사료된다.