• 정보처리학회논문지B
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• 제16B권5호
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• pp.395-402
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• 2009

본 연구는 표정 인식률을 개선하기 위한, 강도가 다른 표정을 인식하는 새로운 표정 인식 방법을 제안한다. 사람마다 다르게 나타나는 표정과 표정마다 다른 강도는 표정 인식률 저하에 지대한 영향을 미친다. 하지만 얼굴 표정의 다양한 강도를 처리하는 방법은 많이 제시되지 않고 있다. 본 연구에서는 표정 템플릿과 표정 강도 분포모델을 이용하여 다양한 얼굴 표정 강도를 인식하는 방법을 제시한다. 표정 템플릿과 표정강도 분포모델은 얼굴의 특징 부위에 표시한 관심 점과 얼굴 특징 부위간의 움직임이 다른 표정과 강도에 따라 어떻게 달라지는지 설명하여 표정 인식률 개선에 기여한다. 제안 방법은 정지 이미지뿐만 아니라 비디오시퀀스에서도 빠른 측정 과정을 통해 다양한 강도의 표정을 인식할 수 있는 장점이 있다. 실험 결과, 제안 연구가 특히 약한 강도의 표정에 대해 타 방법보다 높은 인식 결과를 보여 제안 방법이 다양한 강도의 표정 인식에 강건함을 알 수 있다.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.161-164
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• 2005

Data mining techniques can be applied to identify patterns of interest in the gene expression data. One goal in mining gene expression data is to determine how the expression of any particular gene might affect the expression of other genes. To find relationships between different genes, association rules have been applied to gene expression data set [1]. A notable limitation of association rule mining method is that only the association in a single profile experiment can be detected. It cannot be used to find rules across different condition profiles or different time point profile experiments. However, with the appearance of time-series microarray data, it became possible to analyze the temporal relationship between genes. In this paper, we analyze the time-series microarray gene expression data to extract the sequential patterns which are similar to the association rules between genes among different time points in the yeast cell cycle. The sequential patterns found in our work can catch the associations between different genes which express or repress at diverse time points. We have applied sequential pattern mining method to time-series microarray gene expression data and discovered a number of sequential patterns from two groups of genes (test, control) and more sequential patterns have been discovered from test group (same CO term group) than from the control group (different GO term group). This result can be a support for the potential of sequential patterns which is capable of catching the biologically meaningful association between genes.

• 한국통계학회:학술대회논문집
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• 한국통계학회 2002년도 춘계 학술발표회 논문집
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• pp.85-90
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• 2002

Microarray technology allows the monitoring of expression levels for thousands of genes simultaneously. In time-course experiments in which gene expression is monitored over time we are interested in testing gene expression profiles for different experimental groups. We propose a statistical test based on the ANOVA model to identify genes that have different gene expression profiles among experimental groups in time-course experiments. Using this test, we can detect genes that have different gene expression profiles among experimental groups. The proposed model is illustrated using cDNA microarrays of 3,840 genes obtained in an experiment to search for changes in gene expression profiles during neuronal differentiation of cortical stem cells.

• Animal Bioscience
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• 제35권4호
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• pp.624-630
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• 2022

Objective: The aim of this study was to investigate the expression level of filaggrin-2 (FLG2) in correlation with drip loss. Methods: The muscle samples were randomly taken from a local meat supplier. Samples were taken from Longissimus lumborum muscles to evaluate the drip loss (n = 100). Five muscles per group (low and high drip loss) were selected to evaluate FLG2 mRNA and protein expression levels. Results: mRNA of FLG2 gene was not significantly different in pigs with different levels of drip loss (p>0.05). Statistical analysis revealed that FLG2 protein expression levels were significantly different between the drip loss groups. Western blot revealed that the high drip loss group had higher FLG2 protein expression level than the low drip loss group (p<0.001). Moreover, immunohistochemistry revealed the high signal intensity was on the muscle cell membrane and cytoplasm. Conclusion: FLG2 protein might play roles in drip loss of pork and will provide the basis for information to improving meat quality traits in pigs.

• Journal of Information Processing Systems
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• 제19권3호
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• pp.323-333
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• 2023

Facial expression recognition can aid in the development of fatigue driving detection, teaching quality evaluation, and other fields. In this study, a facial expression recognition method was proposed with a residual masking reconstruction network as its backbone to achieve more efficient expression recognition and classification. The residual layer was used to acquire and capture the information features of the input image, and the masking layer was used for the weight coefficients corresponding to different information features to achieve accurate and effective image analysis for images of different sizes. To further improve the performance of expression analysis, the loss function of the model is optimized from two aspects, feature dimension and data dimension, to enhance the accurate mapping relationship between facial features and emotional labels. The simulation results show that the ROC of the proposed method was maintained above 0.9995, which can accurately distinguish different expressions. The precision was 75.98%, indicating excellent performance of the facial expression recognition model.

• 복식
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• 제66권4호
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• pp.111-127
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• 2016

The study aims to examine the forms of 'image expression' and utilization of model poses in fashion photos, and to delve into the characteristics and the intents that make certain model poses effective in expressing an image. The study used the fashion photos in the fashion magazine, Vogue, to analyze the different model poses used to express different images. The results are as follows. First, image expression forms in fashion photos were categorized into 'direct product suggestion expression form', 'sensual image expression form', 'sexual image expression form', 'story telling expression form', 'everyday situation expression form', and 'fantastic image expression form'. The different utilization types of model poses were categorized into 'type utilizing intangible elements', 'type utilizing complicated elements', 'type utilizing living organisms', 'type utilizing props', 'type utilizing clothes', 'type utilizing location', 'type utilizing accessories', and 'type utilizing products'. Second, the most common expression form for fashion photos used in advertisements was the 'direct product suggestion expression form', which was followed by the sensual image expression form. The most popular form used in the editorial fashion photos was the direct suggestion product expression form, which was followed by the story telling expression form. Third, the most common model pose type for direct product suggestion form was the 'type utilizing product'. Fourth, 'direct product suggestion expression form' was mostly used in editorial fashion photos. The most common utilization types of model poses were 'type utilizing clothes', 'type utilizing props', and 'type utilizing place'.

• 한국작물학회지
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• 제60권1호
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• pp.33-40
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• 2015

재배환경에 따른 단백질 발현을 이차원전기영동을 이용하여 단백질 발현양상을 확인하고 이미지 분석을 통하여 단백질 발현 정도를 수치화하여 비교 분석한 결과, 재배환경에 따른 단백질 발현 양상은 전반적으로 매우 유사하였으며, 주요 단백질의 발현에는 차이가 없었다. 하지만 논과 밭, 즉 재배환경은 단백질의 발현 정도에는 영향을 미치는 것을 확인 할 수 있었다. 공시품종들 중에서 백천콩은 다른 세 품종과는 다르게 논에서 단백질 발현 정도가 밭에서의 단백질 발현 정도보다 높았다. 이 결과로 보아 품종마다 재배환경에 따른 단백질의 발현 정도에 차이가 있는 것으로 사료된다. 향후, 단백질 총 함량을 재배환경에 따라 비교하고 또한 질량분석을 통하여 증가 또는 감소되는 단백질의 기능을 확일 할 필요가 있다고 사료된다.

• 대한의생명과학회지
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• 제17권3호
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• pp.185-190
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• 2011

Adenovirus type 5 (Ad5) vectors have been used for gene transfer to a wide variety of cell types in vivo and in vitro. The advantages of adenovirus vectors include the high titer of virus readily obtained in large scale preparations, their ability to transduce dividing and non dividing cells, and the high level of transgene expression. Since adenovirus vectors do not integrate in host cell DNA, there is a lack of insertional mutagenesis. However, many human tumor cells lack expression of the adenovirus 5 receptors and contain areas of hypoxia. In order to identify the pattern of replication and gene expression of oncolytic adenovirus in hypoxic condition, multiple different fiber modified Ads (Ad5F/S11, Ad5F/S35, Ad5F/K7, Ad5F/K21, and Ad5F/RGD) was compared. The replication of all fiber modified adenovirus was inhibited in hypoxic condition in HEK 293 cells, but gene expression has variety on different tumor cell lines and the level of coxackievirus and adenovirus receptor (CAR) expression. These data suggest that CAR expression pattern and hypoxic condition of tumor are considered for optimal oncolytic adenovirus application.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6341-6345
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• 2013

To clarify the role of stem cells in hepatocarcinogenesis, glypican-3 (GPC-3) and E-cadherin expression was investigated in embryonic cell lineages. Mouse embryonic stem cells (ESCs), hepatic progenitor cells (HPCs) and hepatocyte like cells (HCs), representing 0, 22 and 40 days of differentiation, respectively, were treated in vitro with diethylnitrosamine (DEN) at four doses (0, 1, 5 and 15 mM; G1, G2, G3 and G4, respectively) for 24 h and GPC-3 and E-cadherin expression was examined by relative quantitative real-time PCR and immunocytochemistry. GPC-3 mRNA expression was significantly different for G4 at day 0 (p<0.001) and for G4 at day 22 (p<0.01) compared with the control (G1). E-cadherin mRNA expression was significantly different for G3 and G4 at day 0 (p<0.05 and p<0.001, respectively), for G2 and G4 (p<0.05 and p<0.001, respectively) at day 22 and for G2 and G4 (p<0.01 and p<0.001, respectively) at day 40 compared with G1. Immunofluorescence staining for GPC-3 showed a membranous and/or granular expression in cytoplasm of ESCs and HPCs and granular and/or diffuse expression in cytoplasm of HCs, which were also stained by E-cadherin. DEN treatment increased GPC-3 expression in ESCs, HPCs and HCs, with increase of E-cadherin expression. Taken together, the expression of GPC-3 was altered by DEN treatment. However, its expression pattern was different at the stage of embryo stem cells and its derived hepatic lineage cells. This suggests that GPC-3 expression may be modulated in the progeny of stem cells during their differentiation toward hepatocytes, associated with E-cadherin expression.

• 한국발생생물학회지:발생과생식
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• 제24권3호
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• pp.167-176
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• 2020

The fat pad defined as the epididymal fat is located at the head part of the epididymis. However, another fat mass is present near the caudal epididymis, named tail epididymal fat. The present research was focused to determine the expression of adipocyte-associated molecules in the mouse tail epididymal fat at different postnatal ages, including 2, 5, 8, and 12 months of age. The quantitative real-time PCR analysis showed continuous increases of expression levels of delta like non-canonical Notch ligand 1, leptin, and resistin as postnatally aged. The transcript level of peroxisome proliferator-activated receptor gamma was the highest at 5 months of age, remaining at a steady level until 12 months of age. Expression levels of fatty acid binding protein 4, leptin, and adiponectin were significantly increased until 8 months of age, persisting the level at 12 months of age. The transcript level of fatty acid synthase was significantly increased at 8 months of age, without a further change of the level at 12 months of age. These findings exhibited the expression of adipocyte-associated genes which were also detected at the ordinary epididymal fat pad. However, expression patterns of these genes in the tail epididymal fat are different with those in the distal and proximal epididymal fat, suggesting distinct characteristics and/or functions of the tail epididymal fat.