• Journal of Microbiology and Biotechnology
• /
• v.11 no.4
• /
• pp.585-591
• /
• 2001

To compare the p10 promoter activity of Bombyx mori nucleopolyhedrovirus (BmNPV)K1 and K4, recombinant viruses Bm101-LacZ and Bm104-LacZ with a lacZ gene under the control of each p10 promoter were constructed. The $\beta$-galactosidase activity due to Bm101-LacZ was about 5.5- and 1.1-fold higher than that due to Bm104-LacZ and BmK1-LacZ, respectively. expressing ${\beta}$-galactosidase under the control of a polyhedrin promoter. The recombinant virus BmK1-104LacZ with the same genome structure as Bm101-LacZ, except for a p10 promoter region, produced a similar ${\beta}$-galactosidase activity to that due to Bm104-LacZ and 5.5-fold lower than that due to Bm101-LacZ. The virus yield, expression level of polyhedrin, and polyhedra productivity for each recombinant virus was almost similar. These results suggested that the difference in the expression level of ${\beta}$-galactosidase resulted from a difference in the p10 promoter regions, and that an expression vector using the p10 promoter of BmNPV-K1 could be usefully exploited in the mass production of foreign proteins with silkworm larvae by means of oral ingestion.

• Journal of Digital Convergence
• /
• v.10 no.8
• /
• pp.239-247
• /
• 2012

This paper is related to an Object VR-based virtual textile wearing system carrying out textile texture mapping based on viewpoint vector estimation and intensity difference map. The proposed system is characterized as capable of virtually wearing a new textile pattern selected by the user to the clothing shape section segmented from multi-view 2D images of clothes model for Object VR(Object Virtual Reality), and three-dimensionally viewing its virtual wearing appearance at multi-view points of the object. Regardless of color or intensity of model clothes, the proposed system is possible to virtually change the textile pattern with holding the properties of the selected clothing shape section, and also to quickly and easily simulate, compare, and select multiple textile pattern combinations for individual styles or entire outfits. The proposed system can provide higher practicality and easy-to-use interface, as it makes real-time processing possible in various digital environment, and creates comparatively natural and realistic virtual wearing styles, and also makes semi-automatic processing possible to reduce the manual works.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.48 no.6
• /
• pp.105-114
• /
• 2011

The snake algorithm is widely adopted to track objects by extracting the active contour of the object from background. However, it fails to track the target converging to the background if there exists background whose gradient is greater than that of the pixels on the contour. Also, the contour may shrink when the target moves fast and the snake algorithm misses the boundary of the object in its searching window. To alleviate these problems, we propose an improved algorithm that can track object contour more robustly. Firstly, we propose two external energy functions, the edge energy and the contrast energy. One is designed to give more weight to the gradient on the boundary and the other to reflect the contrast difference between the object and background. Secondly, by computing the motion vector of the contour from the difference of the two consecutive frames, we can move the snake pointers of the previous frame near the region where the object boundary is probable at the current frame. Computer experiments show that the proposed method is more robust to the complicated background than the previously known methods and can track the object with fast movement.

• International Journal of Vascular Biomedical Engineering
• /
• v.1 no.1
• /
• pp.13-23
• /
• 2003

Backgrounds: The present study in angulated coronary stenosis was to evaluate the influence of velocity and wall shear stress (WSS) on coronary atherosclerosis, the changes of hemodynamic indices following coronary stenting, as well as their effect of evolving in-stent restenosis using human in vivo hemodynamic parameters and computed simulation quantitatively and qualitatively. Methods: Initial and follow-up coronary angiographies in the patients with angulated coronary stenosis were performed (n=80). Optimal coronary stenting in angulated coronary stenosis had two models: < 50 % angle changed(model 1, n=43), > 50% angle changed group (model 2, n=37) according to percent change of vascular angle between pre- and post-intracoronary stenting. Flow-velocity wave obtained from in vivo intracoronary Doppler study data was used for in vitro numerical simulation. Spatial and temporal patterns of velocity vector and recirculation area were drawn throughout the selected segment of coronary models. WSS of pre/post-intracoronary stenting were calculated from three-dimensional computer simulation. Results: Follow-up coronary angiogram demonstrated significant difference in the percent of diameter stenosis between two groups (group 1: $40.3{\pm}30.2$ vs. group 2: $25.5{\pm}22.5%$, p<0.05). Negative WSS area on 3D simulation, which is consistent with re-circulation area of velocity vector, was noted on the inner wall of post-stenotic area before stenting. The negative WSS was disappeared after stenting. High spatial and temporal WSS before stenting fell into within physiologic WSS after stenting. This finding was prominent in Model 2 (p<0.01) Conclusions: The present study suggests that hemodynamic forces exerted by pulsatile coronary circulation termed as WSS might affect on the evolution of atherosclerosis within the angulated vascular curvature. Moreover, geometric change, such as angular difference between pre / post-intracoronary stenting might give proper information of optimal hemodynamic charateristics for vascular repair after stenting.

• Journal of Korea Water Resources Association
• /
• v.33 no.5
• /
• pp.611-622
• /
• 2000

In this study, MODFLOW model is used to analyze the groundwater flow system of Seoguipo area in Cheju island, The final parameters of permeability coefficient and storage coefficient of target area can be obtained by trial and error method using the measured data of pumping rate as initial values. And it is found that the applicability for groundwater flow system is reflected well from the simulation result of the model. Seoguipo area spring water is thought to appear by relatively stable groundwater recharge below EL. 400m according to head distribution through the analysis of observed data considering topographic and geological characteristics, Lee's study(996), and the simulation result. Also it is known that point II, III, and VI show relatively large velocity vectors, and groundwater flows through the movement path which is distributed in various directions of I, II, III, IV, V, VI, and VIl form the result of velocity vector analysis using head distribution result values to analyze the groundwater flow path under unsteady flow condition.dition.

• Korean Journal of Optics and Photonics
• /
• v.24 no.2
• /
• pp.77-85
• /
• 2013

The precision of retardation measurement has been improved upto $3{\sigma}$ <0.005 nm after improvements are made to the conventional transmission ellipsometer. Improvements are made such that, i) the polarizer module instead of the sample stage is rotated, ii) the light source is replaced, iii) the starting points of two rotating modules are accurately synchronized, and iv) the fine background retardation is compensated. Together with the newly introduced RVD (Retardation Vector Difference) method, the improved instrument is successfully applied to characterize the ultra small optical birefringence of the rubbed polyimide alignment layer, after the residual retardation due to glass substrate whose magnitude is about 1.0 nm is properly subtracted. It is verified that the net retardation of the alignment layer ranges from 0.05 nm to 0.15 nm.

• Journal of Broadcast Engineering
• /
• v.9 no.4 s.25
• /
• pp.446-454
• /
• 2004

With the growth of multimedia systems, compressing image data has become more important in the area of multimedia services. Since a compressed image bitstream can often be seriously distorted by various types of channel noise, an error concealment algorithm becomes a very important issue. In order to solve this problem, Hsia proposed the error concealment algorithm where he recovered lost block data using 1D boundary matching vectors. His algorithm, however, requires high computational complexity since each matching vector needs MAD (Mean Absolute Difference) values of all pixels, which is either a boundary line top or a boundary line bottom of a damaged block. We propose a hierarchical search-based fast error concealment scheme as well as its approximated version to reduce computational time. In the proposed scheme, a hierarchical search is applied to reduce the number of checking points for searching a vector. The error concealment schemes proposed in this paper can be about 3 times faster than Hsia's with keeping visual quality and PSNR.

• Journal of Broadcast Engineering
• /
• v.10 no.3
• /
• pp.321-327
• /
• 2005

A MVC (Multi-view Video Coding) method, which uses both an illumination change-adaptive ME (Motion Estimation)/DC (Motion Compensation) and a 2D (Dimensional) direct mode, is proposed. Firstly, a new SAD (Sum of Absolute Difference) measure for ME/MC is proposed to compensate the Luma pixel value changes for spatio-temporal motion vector prediction. Illumination change-adaptive (ICA) ME/MC uses the new SAD to improve both MV (Motion Vector) accuracy and bit saving. Secondly, The proposed 2D direct mode that can be used in inter-view prediction is an extended version of the temporal direct mode in MPEG-4 AVC. The proposed MVC method obtains approximately 0.8dB PSNR (Peak Signal-to-Noise Ratio) increment compared with the MPEG-4 AVC simulcast coding.

• Proceedings of the KSRS Conference
• /
• 2002.10a
• /
• pp.615-620
• /
• 2002

Probability distribution of the sea surface slope is estimated using sun glitter images derived from visible radiometer on Geostationary Meteorological Satellite (GMS) and surface vector winds observed by spaceborne scatterometers. The brightness of the visible images is converted to the probability of wave surfaces which reflect the sunlight toward GMS in grids of 0.25 deg $\times$ 0.25 deg. Slope and azimuth angle required for the reflection of the sun's ray toward GMS are calculated for each grid from the geometry of GMS observation and location of the sun. The GMS images are then collocated with surface wind data observed by three scatterometers. Using the collocated data set of about 30 million points obtained in a period of 4 years from 1995 to 1999, probability distribution function of the surface slope is estimated as a function of wind speed and azimuth angle relative to the wind direction. Results are compared with those of Cox and Munk (1954a, b). Surface slope estimated by the present method shows narrower distribution and much less directivity relative to the wind direction than that reported by Cox and Munk. It is expected that their data were obtained under conditions of growing wind waves. In general, wind waves are not always developing, and slope distribution might differ from the results of Cox and Munk. Most of our data are obtained in the subtropical seas under clear-sky conditions. This difference of the conditions may be the reason for the difference of slope distribution.

• Korean Journal of Applied Biomechanics
• /
• v.31 no.3
• /
• pp.205-213
• /
• 2021

Objective: The aim of this study was to analyze body stability Joint coordination pattern though as bending stiffness of shoes during stance phase of running. Method: 47 male subjects (Age: 26.33 ± 2.11 years, Height: 177.32 ± 4.31 cm, Weight: 65.8 ± 3.87 kg) participated in this study. All subjects tested wearing the same type of running shoes by classifying bending stiffness (A shoes: 3.2~4.1 N, B shoes: 9.25~10.53 N, C shoes: 20.22~21.59 N). They ran 10 m at 3.3 m/s (SD ±3%) speed, and the speed was monitored by installing a speedometer at 3 m intervals between force plate, and the measured data were analyzed five times. During running, ankle joint, MTP joint, coupling angle, inclination angle (anterior-posterior, medial-lateral) was collected and analyzed. Vector coding methods were used to calculate vector angle of 2 joint couples during running: MTP-Ankle joint frontal plane. All analyses were performed with SPSS 21.0 and for repeated measured ANOVA and Post-hoc was Bonferroni. Results: Results indicated that there was an interaction between three shoes and phases for MTP (Metatarsalphalangeal) joint angle (p = .045), the phases in the three shoes showed difference with heel strike~impact peak (p1) (p = .000), impact peak~active peak (p2) (p = .002), from active peak to half the distance to take-off until take-off (p4) (p = .032) except for active peak~from active peak to half the distance to take-off (p3) (p = .155). ML IA (medial-lateral inclination angle) for C shoes was increased than other shoes. The coupling angle of ankle angle and MTP joint showed that there was significantly difference of p2 (p = .005), p4 (p = .045), and the characteristics of C shoes were that single-joint pattern (ankle-phase, MTP-phase) was shown in each phase. Conclusion: In conclusion, by wearing high bending stiffness shoes, their body instability was increased during running.