Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Comparison Promoter Activity of the p10 Gene between Bombyx mori Nucleopolyhedrovirus Variants

  • Hong, Hye-Kyung (Graduate School of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Jae-Young (Graduate School of Agricultural Biotechnology, Seoul National University) ;
  • Woo, Soo-Dong (Graduate School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Hae-Kwang (Graduate School of Agricultural Biotechnology, Seoul National University) ;
  • Je, Yeon-Ho (Graduate School of Agricultural Biotechnology, Seoul National University)
  • Published : 2001.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

To compare the p10 promoter activity of Bombyx mori nucleopolyhedrovirus (BmNPV)K1 and K4, recombinant viruses Bm101-LacZ and Bm104-LacZ with a lacZ gene under the control of each p10 promoter were constructed. The $\beta$-galactosidase activity due to Bm101-LacZ was about 5.5- and 1.1-fold higher than that due to Bm104-LacZ and BmK1-LacZ, respectively. expressing ${\beta}$-galactosidase under the control of a polyhedrin promoter. The recombinant virus BmK1-104LacZ with the same genome structure as Bm101-LacZ, except for a p10 promoter region, produced a similar ${\beta}$-galactosidase activity to that due to Bm104-LacZ and 5.5-fold lower than that due to Bm101-LacZ. The virus yield, expression level of polyhedrin, and polyhedra productivity for each recombinant virus was almost similar. These results suggested that the difference in the expression level of ${\beta}$-galactosidase resulted from a difference in the p10 promoter regions, and that an expression vector using the p10 promoter of BmNPV-K1 could be usefully exploited in the mass production of foreign proteins with silkworm larvae by means of oral ingestion.

Keywords

References

  1. Arch. Virol. v.145 High-level expression of canine parvovirus VP2 using Bombyx mori nucleoplyhedrovirus vector Choi, J.Y.;S.D. Woo;H.K. Lee;H.K. Hong;Y.H. Je;J. H. Park;J. Y. Song;S. H. An;S. K. Kang
  2. The Biology of Baculoviruses Granados, R. R.;B. A. Federici
  3. J. Microbiol. Biotechnol v.8 Expression and characterization of recombinant E2 protein of Hepatitis C virus by insect cell/baculovirus expression system Han, B.K.;B.Y. Lee;M.K. Min;K. H. Jung
  4. Arch. Virol. v.145 Characterization of four isolates of Bombyx mori nucleopolyhedrovirus Hong, H.K.;S.D. Woo;J.Y. Choi;H,K. Lee;M. H. Kim;Y. H. Je;S.K. Kang
  5. J. Gen. Virol. v.75 The p10 gene of natural isolates of Bombyx mori nuclear polyhedrosis virus encodes a truncated protein with an Mr of 7700 Hu, N. T.;Y.F. Lu;Y. Hashimoto;S. Maeda;R.F. Hou
  6. J. Microbiol. Biotechnol. v.8 cDNA cloning and expression of human rotavirius outer capsid protein VP7 in insect cells Kang, D.K.;K. W. Kim;P. H. Kim;S. Y. Seoung;Y. H. Kim;I.C. Kwon;S. Y. Jeong;E. Y. Choi;K. M. Lee;H. S. Kim;E. C. Kim;S. I. Joo;J. M. Yang
  7. The Baculovirus Expression System: A Laboratory Guide King, L. A.;R. D. Possee
  8. Cytotechnology v.8 High level expression of a frog α-amidating enzyme, AE-Ⅱ, in cultured cells and sikworm larvae using a Bombys mori nuclear polyhedrosis virus expression vector Kobayashi, J.;S. Imanishi;H. Inoue;K. Oshue;K. Yamaichi;N. Tsuruoka;S. Tanaka
  9. J. Microbiol. Biotechnol. v.9 Expression of human thrombopoietin in insect cells by polyhedrin-gp64 dual promoter-based baculovirus vector system Koh, Y.W.;S.K. Park;K.S. Kim;S.R. Kim;J. K. Lee;J.M. Yang
  10. Bio/Technology v.6 Trends in the development of baculovirus expression vectors Luckow, V.L.;M.D. Summers
  11. Annu. Rev. Entomol v.34 Expression of foreign genes in insects using baculovirus Maeda, S.
  12. Insect Cell Biotechnology. Expression of foreign gene in insect cells using baculovirus vectors Maeda, S.;K. Maramorosch(eds.);A. McIntosh(eds.)
  13. Nature v.315 Production of human α-interferon in silkworm using a baculovirus vector. Maeda, S.;T. Kawai;M. Obinata;H. FujiwaralT. Horiuchi;Y. Saeki;Y. Soto;M. Furusawa
  14. Annu. Rev. Microbiol. v.42 Baculoviruses as gene expression vectors Miller, L.K.
  15. J. Gen. Virol. v.72 Transcriptional analyses of baculovirus polyhedrin and foreign gene expression relative to baculovirus p10 mRNA levels Min, M.K.;D.H.L. Bishop
  16. Gene v.58 Use of silkworm Bombyx mori, and insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3 Miyajima, A.;J. Scheurs;K. Otsu;A. Kondo;K. Arai;S. Maeda
  17. Baculovirus Expression Vectors: A Laboratory Manual O'Reilly, D.R.;L. K. Miller;V. A. Luckow
  18. Bio/Technology v.10 antibody production in silkworm cells and silkworm larvae infected with a dual recominant Bombyx mori nuclear polyhedrosis virus Reis, U.;B. Blum;B. R. von Specht;H. Domdey;J. Collins
  19. J. Gen. Virol. v.73 Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes Roelvink, R.W.;M.M.M. van Meer;C.A.D. de Kort;R.D. Possee;B.D. Hammock;J.M. Vlak
  20. J. Virol v.44 In vitro translation of autographa californica nuclear polyhedrosis virus early and late mRNAs. Smith, G.E.;J.M. Vlak;M.D. Summers
  21. Cytotechnology v.17 Isolation of p10 gene from Bombyx mori nuclear polyhedrosis virus and study of its promoter activity in recombinant baculovirus vector system Tomita, S.;T. Kanaya;J. Kobayashi;S. Imanishi
  22. J. Gen. Virol. v.74 Functional domains of the p10 protein of Autographa califormica nuclear polyhedrosis virus van Oers, M. M.;J.T.M. Flipsen;C.B.E.M. Reusken;E.L. Sliwinsky;R.W. Goldbach;J.N. Vlak
  23. J. Invertebr.Pathol v.70 The baculovirus 10-kDa protein van Oers, M. M.;J. M. Vlak
  24. J. Virol. v.64 Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the β-galactosidase gene Vialard, J.;M. Lalumiere;T. Vernet;D. Briedis;G. Alkhatib;D. Henning;D. Levin;C. Richardson
  25. Virology v.179 Expression of cauliflower mosaic virus gene using a baculovirus vector based upon the p10 gene and a novel selection method Vlak, J.M.;A. Schouten;M. Usmany;G.J. Belsham;E.C. Klinge-Roode;A.J. Maule;J.W.M. Lent;D. Zuidema
  26. J. Gen. Virol. v.71 Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectors Weyer, U.;S. Knight;R. D. Possee
  27. Nucleic Acids Res. v.16 Functional analysis of the p10 gene 5' leader sequence of the Autographa californica nuclear polyhedrosis virus Weyer, U.;R.D. Possee
  28. J. Virol. v.70 Analysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene Weyer, U.;R.D. Possee
  29. Kor. J. Appl. Microbiol Biotechnol v.24 Expression of Escherichia coli β-galactosidase gene by new transfer vector of baculovirus Woo, S. D.;W.J. Kim;H.S. Kim;B.R. Jin;S.K. Kang
  30. J. Microbiol. Biotechnol. v.2 Phosphorylation of nucleocapsid protein of bovine coronavirus expressed with a recombinant baculovirus vector Yoo, D.W.;J.C. Graham;I.D. Yoo
  31. Sci. China B, Chem. Life Sci. Earth Sci. v.38 P10 genes of Bambyx mori nuclear polyhedrosis virus and Autographa californica multiple nuclear polyhedrosis virus Zhang, Y.;X. Wu;Z.Lo
  32. Sci. China B, Chem. Life Sci. Earth Sci. v.38 P10 genes of Bombyx mori nuclear polyhedrosis virus and Autographa californica multiple unclear polyhedrosis virus Zhang,Y.;X.Wu;Z.Li