• Journal of fish pathology
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• v.17 no.2
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• pp.151-157
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• 2004

Application of a commercial Diff-Quick kit for fish hematology was evaluated. Diff-Quick stain was incomplete in staining not only the nucleus and cytoplasm but also granules of leucocytes that were stained in soluton I and II for five seconds. However, when the staining time changed to 5 seconds in solution I and 10 seconds in solution II, followed by washing with deionized distilled water, the kit showed the similar results as in May-Grunwald Giemsa stain. The results indicate that the kit can be used in fish hematology for rapid staining.