• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.191-200
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• 2011

Four ruminally cannulated Hanwoo steers (BW 600 ${\pm}$ 48.4 kg) fed whole crop rice silage (WRS) as forage were used to investigate the effects of its dietary on rumen fermentation and blood metabolites at different harvesting time such as milk, dough, yellow ripe and mature stages of WRS, respectively. Mean ruminal pH sampled at 0, 1, 2, 4, 6 and 8 h after the morning feeding was not significantly (p>0.05) different between the WRS by the harvesting time. In diurnal pattern in ruminal pH, the lowest pH for the mature stage treatment (Mature) appeared at 6 h after the feeding while it appeared at 2-4 h for the other treatments. This may indicate that retention time in the rumen of Hanwoo fed WRS harvested at the Mature increased. Although feeding WRS harvested at different stages did not significantly (p>0.05) affect ruminal ammonia N concentration, the ammonia N for WRS harvested at the milk stage (Milk) tended (p=0.11) to decrease compared with that for the yellow stage treatment (Yellow). In the blood metabolites analysis, BUN concentration for Yellow numerically decreased compared with that for Milk. This, taking lower concentration of ruminal ammonia N for Milk than the other treatments into account, clearly indicates that the BUN concentrations may not increase in proportion to ruminal ammonia N concentration according to ammonia N concentration entering into the lower gastric tracts. Lower (p=0.03) concentration of blood albumin for the dough stage treatment than that for Mature may indicate high protein synthesis in Hanwoo fed WRS at the dough stage, but further studies in terms of mechanism of nutrients distribution should be required. Present results indicate that different harvesting time did not affect rumen fermentation and blood metabolites in Hanwoo. Thus, based on the results of the present and the previous studies, utilizing WRS harvested at yellow stage could be recommendable for Hanwoo steers.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.172-182
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• 2016

This study was aimed to determine the changes in vitamin $B_5$ and $B_6$ contents compared to fresh materials after parboiling treatment of the main vegetables consumed in Korea. The specificity of accuracy and precision for vitamin $B_5$ and $B_6$ analysis method were validated using high-performance liquid chromatography (HPLC). The recovery rate of standard reference material (SRM) was excellent, and all analysis was under the control line based on the quality control chart for vitamin $B_5$ and $B_6$. The Z-score for vitamin $B_6$ in food analysis performance assessment scheme (FAPAS) proficiency test was -1.0, confirming reliability of analytical performance. The vitamin $B_5$ and $B_6$ contents in a total of 39 fresh materials and parboiled samples were analyzed. The contents of vitamin $B_5$ and $B_6$ ranged from 0.000 to 2.462 and from 0.000 to $0.127mg{\cdot}100g^{-1}$, respectively. The highest contents of vitamin $B_5$ and $B_6$ were $2.462mg{\cdot}100g^{-1}$ in fresh fatsia shoots (stem vegetables), and $0.127mg{\cdot}100g^{-1}$ in fresh spinach beet (leafy vegetables), respectively. Moreover, the vitamin $B_5$ and $B_6$ contents for parboiling treatment in most vegetables were reduced or not detected. In particular, the contents of vitamin $B_5$ in parboiled fatsia shoots and vitamin $B_6$ in parboiled yellow potato and spinach beet were decreased 20- and 4-fold compared with fresh material, respectively. These results can be used as important basic data for utilization and processing of various vegetable crops, information for dietary life, management of school meals, and national health for Koreans.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.4
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• pp.446-454
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• 2008

The traditional Korean drink "Jehotang", which is one of the most commonly prescribed drugs for treatment of the heat stroke accompanied by high fever, severe sweat and thirst, it is effective far quenching thirst strengthening the stomach, stopping diarrhea and regulating intestinal function. It is also known to play a role in activating the growth of useful microbes in the intestine and in multiplying intestinal immune cells. Thus, this study investigated all aspects of the drink in an effort to develop a new functional beverage. In the course of this study, the analytical research into the literature concerning Jehotang an error in the secrets of preparing the drink. The Japanese apricot, which was given the botanical name, "Prunusmume", should only be used with the flesh (scientifically referred to as "Fructusmume"), which is fumigated with straw fire before drying. and the seed should be thrown away. The honey should only be used after it is heated with a gentle fire, before removing the white foam that farms on its surface to make the "Yeonmil". Two kinds of cooking processes were found in the ancient literature. One procedure boiling down the powered Fructus mume mixed with the Yeonmil, while the other procedure involved preparing the Fructus mume's water by adding water to it and blending the water with the Yeonmil and the rest of the raw ingredient before boiling them down. The current procedure, in which the cooking is done in a double boiler, has been widely adopted to its simplicity. The finished Jehotang is put in porcelain, kept at room temperature, and consumed after mixing with cold water.

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.955-962
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• 2005

This study was conducted to examine the nutrient digestibility and ruminal fermentation characteristics in sheep fed diets containing four substitution levels of E. ulmoides leaves for rice straw. For the experiment, they were given a basal diet consisting of rice straw and concentrate at a 3:7 ratio (DM basis). The treatments were designed as a 4$\time$4 Latin square design with four sheep (50.2$\pm$1.6 kg body weight). Sheep were allotted in one of four treatments, which were designed to progressively substitute Eucommia ulmoides for 0, 3, 5 and 10% of rice straw in the basal diet. The digestibility of crude fat was significantly (p<0.05) improved by 12.5 to 17.5% in 3% and 5% air dried Eucommia ulmoides leaves compared with that in control. The digestibility of crude fiber in the 3, 5 and 10% treatments was significantly (p<0.05) higher than that of the control. Dry matter intake was highest (p<0.05) in sheep fed 10% air dried E. ulmoides). Retained nitrogen in the 3, 5 and 10% treatments was increased up to 97%, 173% and 192%, respectively, compared to that in control (p<0.05). Organic matter utilization was higer in 3% and 5% treatments than control (p<0.05). Purine derivatives and microbial nitrogen synthesis were significantly increased by the dietary substitution of 5 % E. ulmoides leaves (p<0.05) compared with control and the 3% treatment. Ruminal concentration of total-VFA was significantly (p<0.05) higher in sheep fed all levels of air dried Eucommia ulmoides than those of control. It is concluded that air-dried E. ulmoides leaves given to ruminants can increase nutrient digestibility and palatability and improve ruminal fermentation. Therefore, the 5 and 10% substitution of E. ulmoides for roughage are highly recommended to be used in practice.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.611-620
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• 2007

The current study was designed to define whether a blend of prunus mume extract(25%) containing lactic acid(75%) and grape seed extract(10ppm) could affect in vitro antimicrobial activity and growth performance, intestinal microflora, plasma biochemical profiles and digestive enzymes activities in broiler chickens. In paper disc agar diffusion test, we clearly observed antimicrobial activity against E. coli in response to prunus mume extract or a blend of prunus mume extract. For in vivo test, a total of ninety six 3-d-old male broiler chicks were assigned to basal diet(CON), basal diet supplemented with antibiotics (ANTI) and 0.5% a blend of prunus mume extract(PRNUS) until 35 days of age. Throughout the entire experimental period(3-35 days), there were no differences in BW and FCR between the birds fed the basal diet with antibiotics and the diet supplemented with a blend of prunus mume. However, ANTI group showed a significant increase in BW and total gain compared to CON group. The weights of digestive organs such as the pancreas and mucosal tissues were not affected by dietary treatments. There was no difference in plasma levels of glucose, cholesterol, AST and ALT activity. However, triglyceride in plasma increased(P<0.05) in the birds fed the diet supplemented with 0.5% a blend of prunus mume extract compared to those fed antibiotics supplemented diet. The activities of pancreatic trypsin and amylase, and intestinal hydrolase including disaccharidase were not affected by dietary treatment. The colony forming units(CFU) of lactobacillus in the lower ileal-cecum of the birds fed the diet supplemented with a blend of prunus mume extract was significantly(P<0.05) higher than that of birds fed antibiotic supplemented diet without affecting the CFU of E. coli. In conclusion, the birds fed the diet supplemented a blend of prunus mume as an alternative to antibiotics showed a similar growth performance and an significant increase in lactobacillus population compared with the birds fed basal and antibiotics supplemented diets.

• Journal of the East Asian Society of Dietary Life
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• v.21 no.6
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• pp.853-862
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• 2011

We reproduced and investigated the quality characteristics of Samhaeju (one type of Korean traditional rice wine), which has been described in ancient documents. During fermentation, the room temperature was $9.1{\sim}25.0{\circ}C$, and each treatment's material temperature was $11.7{\sim}23^{\circ}C$. As the fermentation proceeded, the pH rapidly decreased (initial pH was 6.6~6.9) and rose gradually from the 18th day. Titratable acidity and amino acidity slowly increased in general. Regarding soluble solid contents, there were various change patterns depending on the production method, and they were affected by initial room temperature. In the treatments using 1 (Sang-ga-yo-rok), 3 (Eum-sik-di-mi-bang), 7 (Jo-sun-mu-ssang-sin-sik-yo-ri-je-beop), 8 (Sang-ga-yo-rok $15^{\circ}C$), reducing sugar contents decreased rapidly after 1st mashing day and then increased slightly after 2nd mashing. The alcohol content increased as the fermentation proceeded, and most of the treatments produced 1/2 the amount of total alcohol content before the 2nd mashing day, followed by a slight increase until the end of fermentation (about 100 days from the 2nd mashing day). In the sensory evaluation, Samhaeju using methods 2, 4, 5, and 6 got high scores and had much reducing sugar contents than other treatments. Among the seven manufacturing processes, method 2 was relatively simple and got the highest score in the sensory evaluation. Therefore, method 2 would be suitable for industrialization and popularization of Korean traditional alcoholic beverage.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.1
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• pp.149-154
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• 2002

Seventy two Duroc $\times$ Yorkshire $\times$ Landrace pigs (75.66$\pm$1.86 kg average initial body weight) were used in a 45 days growth assay to determine the effects of Astragalus membranaceus, ginseng and onion complex on growth performance and carcass characteristics of finishing pigs. Dietary treatments included 1) Control (basal diet), 2) AGO 0.25 (basal diet + 0.25% Astragalus membranaceus, ginseng and onion complex), 3) AGO 0.50 (basal diet+0.50% Astragalus membranaceus. ginseng and onion complex). For overall period, average daily weight gain increased as the concentration of Astragalus membranaceus, ginseng and onion complex in the diets was increased (linear effect, p<0.01). Gain/feed improved without significant difference (p>0.05) as the concentration of Astragalus membranaceus, ginseng and onion complex in the diets was increased. As adding level of Astragalus membranaceus, ginseng and onion complex increased in the diets, A grade appearances of carcass tended to increase (linear effect, p<0.01). The total and LDL+VLDL cholesterol concentrations in serum of pigs fed AGO 0.25 diet were lower than them of pigs fed Control and AGO 0.50 diets without significant difference (p>0.05). $L^{*}$-, $a^{*}$-, and $b^{*}$- value of M. longissimus dorsi muscle were not significantly different among the treatments (p>0.05). Total feed cost per kg of weight gain was lower in the AGO 0.50 treatment (813 won) than in the Control (830 won). The results obtained from this feeding trial suggest that the Astragalus membranaceus, ginseng and onion complex supplementation for finishing pigs had improved growth performance. However, carcass characteristic was not affected by supplemental Astragalus membranaceus, ginseng and onion complex.lex.x.

• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.213-223
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• 2002

This study was conducted to investigate the influence of feeding various Lactobacillus on production performance, nutrients digestibility, intestinal microflora, and fecal $NH_{3}$ gas emission in laying hens. Three hundred and sixty ISA Brown layers, 21 weeks of age, were randomly allotted to nine treatments, with low replicates per treatment. Nine treatments consisted of Control(no Lactobacillus), Lactobacillus crispatus avibrol(LCB: KFCC-11195), Lactobaciilus reuteri avibro2(LRB: KFCC-11196), Lactobacillus crispatus avihenl(LCH: KFCC-11197), Lactobacillus vaginalis avihen2(LVH: KFCC-11198). Each Lactobacillus was added at two levels ($10^{4}$and $10^{7}$ cfu/g diets). Egg production, and egg weight were measured daily. A metabolism trial was conducted following the 12-week feeding trial, during which egg qualities, intestinal microflora and fecal $NH_{3}$ gas emission were examined. Egg production and daily egg mass improved significantly by the addition of various Lactobacillus(P<0.05), of which effect was more notable during the latter part of the feeding trial. But, no significant differences were found among Lactobacillus strains and between two levels of supplementation. Egg weight and feed intake showed no difference among all treatments. Feed conversion ratio of birds fed lactobacillus was significantly improved compared to that of the Control(P<0.05), but not different among lactobacillus treatments. Digestibility of crude protein, ether extract and crude ash improved significantly in lactobacillus treatments(P<0.05). However, there were not statistically different by adding levels and strains. Total counts of Lactobacillus spp. in ileum of layers fed Lactobacillus were significantly higher than that of the control, but no consistent trend was found in cecum. There were no significant differences in intestinal yeast and anaerobes counts among all treatments. The Lactobaciilus supplementation did not exert my effect on the eggshell quality and Haugh unit. Fecal $NH_{3}$ gas emission decreased significantly in Lactobacillus treatments, and showed no difference between the two supplementation levels. From the result of this study, it could be concluded that dietary supplementation of Lactobacillus, regardless of their species, Improves the laying performance and decreases the fecal ammonia gas emission. The proper level of supplementation appears to be $10^{4}$ cfu/g of diet.

• Korean Journal of Poultry Science
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• v.37 no.4
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• pp.433-438
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• 2010

This study investigated the effects of dietary supplementation of the fermented by products garlic and onion on growth performance, blood composition and cecal microflora in broiler chickens. A total four hundred eighty, day old broiler chickens (Ross) were randomly divided into four groups with four replicates of thirty birds each. The treatment groups were negative group (NC, antibiotic-free diet), positive group (PC, basal diet with 0.05% and 0.03% anticoccidials), fermented of onion by product 1.0% group (T1) and fermented of onion by product 1.0% group (T2). The body weight of broilers fed the diets containing fermented by products garlic was higher than the other treatments during overall period. No significant difference were observed on serum chemical composition and blood corpuscle. In the cecal microflira of broiler, the population of the Lactic acid bacteria was showed the higher in chicken fed diets supplemented with fermented of garlic group than other groups (P<0.05). These results suggest the possibility that fermented of garlic and onion by product could be used as the alternative of antibiotics growth promotor of broiler chickens.

• Korean Journal of Poultry Science
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• v.36 no.4
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• pp.287-292
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• 2009

The objective of the current study was to evaluate the effects of garlic extract (GE) supplementation on growth performance, nutrient digestibility, carcass characteristics and meat composition in broilers. Dietary treatment were control (corn-soy based diet, 0 ppm), and diets added with 100 or 200 ppm GE. Starter and finisher diets were fed from 0 to 3 wk and 4 to 5 wk, respectively. Supplementation of GE had no effect on weight gain, but there was trend towards improvement in growth rate in birds fed diets containing 100 ppm GE during overall period, while birds fed 100 ppm GE had better feed efficiency (p<0.05) than birds fed control diet during finisher period. Birds fed GE had greater (p<0.05) nutrient digestibility of dry matter than birds fed control diet. The TBARS values of breast muscle obtained from birds fed 100 and 200 ppm GE were lower than breast muscle of control birds on day 8 and 12 of storage. The breast muscle of birds fed 200 ppm GE had higher (p<0.05) $L^*$ values than breast muscle of birds fed control and 100 ppm GE diets. The carcass characteristics, meat and bone composition, CIE $a^*$ and $b^*$ values did not differ among the dietary treatments. In conclusion, supplementation of GE enhanced feed efficiency and nutrient digestibility in broilers.