• Korean Journal of Community Nutrition
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• v.19 no.2
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• pp.142-162
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• 2014

Objectives: To evaluate the critical environmental factors on healthy-aging of Korean people, we investigated the significant factors influencing health status of septuagenarians living in rural area of Jeonla province, known to be one of the representative longevity regions in Korea. Methods: We divided subjects into healthy group (36M/25F) or poor-health group (26M/73F) based on self-reported health status, body mass index, a number of prescription, and blood test data. General characteristics, physical measurements, lifestyle, dietary behavior and nutrient intake, physical health and mental health data were statistically compared between the two groups. Results: Average age was not different between healthy group and poor-health group in men and women, respectively. In men, significantly favorable factors to health were observed to be higher education, regular exercise, higher grip strength and walking function, body mass index (${\geq}18.5kg/m^2$), moderate frequency of drinking and eating-out, non-smoking, normal red blood cell (RBC) count, higher serum dehydroepiandrosterone-sulfate (DHEAS) level, good digestive function and appetite, normal hearing function, regular meals, adequate vegetable and fruit intake, diverse food intake, adequate energy and nutrients (protein, vitamin $B_1$, $B_6$, C and E, folate, niacin, P, Zn and K) intake, higher mini-nutrient status assessment (MNA) score and low level of depression. On the other hand, in women, those were literacy, living arrangement, moderate frequency of drinking, healthy teeth, higher grip strength and walking function, bone mineral density, normal RBC and white blood cell (WBC) count, higher DHEAS concentration, higher MNA score, normal cognition and memory function, having snack and adequate fruit intake. Conclusions: These results could be useful to plan effective strategies to increase health-life expectancy of Korean old people living in rural areas.

• Journal of Life Science
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• v.26 no.2
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• pp.247-252
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• 2016

Cultivated wild ginseng is a widely used dietary supplement and medicinal herb. The aim of this study was to optimize the ginseng using high performance liquid chromatography (HPLC)- charged aerosol detection (CAD) for ginsenoside analysis. CAD measures the physical property of an analyte and responds to almost all non-volatile species, independent of their nature, spectral properties, or particle size. It has become widely employed in pharmaceutical analysis. The cultivated wild ginseng extracts were analyzed for compositions of ginsenosides Rb1, Rd, Rg1, Rf, Re, and Rh1. The optimal analysis condition was set up from an experiment using a gradient. Ten grams of cultivated wild ginseng were extracted with 95% EtOH 100 ml for 24 hr at 80℃. The contents of the 6six major ginsenosides in the cultivated wild ginseng extract were Rb1 (5.48±0.12 mg/g), Rd (5.33±0.14 mg/g), Rg1 (12.80± 0.05 mg/g), Rf (19.08±0.68 mg/g), Re (19.87±0.05 mg/g), and Rh1 (16.47±0.16 mg/g), respectively. HPLC showed that the protopanaxatriol group (Rg1, Rf, Re, Rh1) had more content than the protopanaxadiol group (Rb1, Rd) in cultivated wild ginseng extract. In summary, the ginsenosides were identified with HPLC-CAD analysis, and their presence and quantity imply the importance of quality control, as well as the pharmacological activity of the ginseng root.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.420-426
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• 2007

In the present investigation, we studied the modulating effects of (-)-epigallocatechin-3-gallate(EGCG) on the differentiation of mouse C2C12 myoblasts. We found that the strong inhibitory effect of EGCG on DNA methyltransferase-mediated DNA methylation induced transdifferentiation of C2C12 myoblasts into smooth muscle cells demonstrated by both morphological changes and immunofluorescent staining. C2C12 myoblasts treated with EGCG for 4 days expressed smooth muscle ${\alpha}-actin$ protein. Real-time PCR data revealed that smooth muscle ${\alpha}-actin$ mRNA was induced by EGCG treated C2C12 myoblasts in a concentration-dependent manner. Smooth muscle ${\alpha}-actin$ mRNA concentration increased 330% and 490% after 2 and 3 days of 50 ${\mu}M$ of EGCG treatment. The expression of another smooth muscle marker, transgelin, mRNA was also increased up to 9-fold by 4 days of EGCG treatment compared with control in a concentration-dependent manner. These results suggested that C2C12 enables to transdifferentiate into smooth muscle when gene expression patterns are changed by the inhibition of DNA methylation induced by EGCG. In conclusion, transdifferentiation of C2C12 myoblasts into smooth muscle is resulted from the modulating effects of EGCG on DNA methylation which subsequently results in changing the expression pattern of several genes playing a critical role in the differentiation of C2C12 myoblasts.

• Journal of fish pathology
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• v.12 no.1
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• pp.7-15
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• 1999

In order to investigate the immune response induced by supplementation of herb medical stuff in diet on the nile tilapia. Oreochromis niloticus, experiments were performed with feeding of four different experimental diets supplemented with 2% ginseng. Panax ginseng, 3% Kugija. Lycium chinense, 3% Hasuo, Polygonum multiflorum, 2% Omija, Schizandra chinensis, respectively, for 84 days. The non-specific immune responses changed during the feeding period were investigated at 2, 4, 6, 8, 10, 12 weeks in each group. Average body weight of the nile tilapia with supplemented diets was heavier than control group. The fish fed on diet supplemented with 3% Kugija showed the better growth than the other tested groups. Complement activity such as complete hemolytic activity ($CH_{50}$) and bactericidal activity against Escherichia coli tended to be increased by the supplementation of herb medical stuff. The lysozyme activity of serum and adherent phagocyte activity showed higher in the fish fec on diet supplemented with 3% kugija than the other tested groups. In respect to the RPS against experimental Edwardsiella tarda infection, all of the group fed on the herb medical stuff in diet appeared higher response compared with control group. From these results, herb medical stuff (Panax ginseng, Lycium chinense. Polygonum multiflorum, Schizandra chinensis) might be used a additives of diet for the increasing of non-specific immune response or resistance against bacterial fish diseases.

• Journal of fish pathology
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• v.23 no.2
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• pp.189-198
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• 2010

Experiments were carried out to determine the hematological changes in olive flounder, Paralichthys olivaceus, after sub-chronic dietary pentachlorophenol (PCP) concentrations (0, 0.1, 0.5 and 1.0 mg/kg) exposure 6 weeks. The RBC count, Ht and Hb were significantly decreased in PCP 0.5 mg/kg exposed flounder compared to control group. The flounder exposed to PCP concentration 0.5 mg/kg started to increase significantly in serum magnesium concentrations after 4 weeks. The low concentration of serum calcium concentration was observed at 6 weeks of the experiment in PCP concentration 0.5 mg/kg. Although serum total protein concentrations was significantly reduced compared to the control group at PCP concentration 0.5 mg/kg, serum glucose concentration was found to be significantly increased over the control group. A significant increment of GOT, GPT and LDH activities in the flounder serum was noticed after 4 weeks at PCP concentrations over 0.5 mg/kg without typical changes of Al-P. These results indicate that flounder can be affected by PCP in terms of inorganic matter, organic matter and enzyme activity in serum when they were exposed to the PCP concentrations 0.5 mg/kg or higher.

• Journal of Distribution Science
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• v.9 no.3
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• pp.55-64
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• 2011

This study is important in its focus to find key clues in the marketing strategy, consumer behavior, and communication processes that define the infantile obesity market. The study, the first of its kind, surveyed a target audience, purchasing group, and housewives in their quest to determine purchasing decisions and effective branding strategy planning for the infantile obesity market. Another key component of the study was to focus on the key direct and/or indirect distribution channels for the subject market. Recently, obesity has emerged as a major social concern; some studies show that the onslaught of an adverse eating culture in Korea emanates from the prevalence of fast-food dining establishments. Obesity among children leads to adult obesity, especially if the young people's parents are overweight; notably, if either one or both of the parents are obese, the percentage of young people eventually being obese is approximately 80 to 85 percent. Because obesity is the cause of many major health concerns later in life, the struggle for a healthy life is considerably adversely affected by parents' consumer behavior. Infantile obesity, resulting in adult obesity, is also an important national economic and social issue. The sizable direct and indirect economic costs, as well as the tremendous social costs of obesity, cannot be overstated. Effective food branding and advertising centered on food preferences and dietary behaviors, especially to children, creates an effective marketing effort that, ultimately, leads to positive results. Thus, the purpose of this study is to demonstrate that the treatment of childhood obesity in Korea, through the activation of a brand and retail market, can effectively solve social and economic problems that result from infantile and childhood obesity. In this study, obesity markets and distribution channels in the purchase decision-making factors determining factor based on it effective inspection and branding strategies and brand marketing communications strategy proposed measures contribute to the obesity drug market and further enable the childhood obesity problem is intended to assist in solving.

• Journal of agriculture & life science
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• v.45 no.1
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• pp.79-88
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• 2011

A total of one hundred twenty pigs were used to investigate effects of silage feeding on pork quality among three breeds (Gagoshima Berkshire, American Berkshire, $LY{\times}\;D$). Pigs weighing about 70 kg of body weight were randomly alloted into three breeds [B (Gagoshima Berkshire), D $(LY{\times}\;D)$, A (American Berkshire) by two dietary treatment [C (commercial diet feed) and T (commercial diet with 80% silage)]. All pigs were fed 67 days of period and then slaughtered to examine meat characteristics. A chemical compositions and physico-chemical characteristics were measured in pork loin. The crude protein content was higher in pig fed diets containing silage than that of the control group. $L^*$ of meat color, appearance, meat color, marbling score and overall acceptability in sensory score of fresh meat were higher in the pigs fed silage (T) than those fed commercial diet (C). In fatty acid compositions of loin, stearic acid and oleic acid were lower in T than C, whereas arachidonic acid, EFA (essential fatty acid) and EFA/UFA (unsaturated fatty acid) were higher in T.

• Journal of agriculture & life science
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• v.45 no.4
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• pp.95-103
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• 2011

Ninety five Hanwoo steers averaging 226kg of body weight were used to investigate the effect of dietary type on feed efficiency, carcass characteristics and economical analysis in Hanwoo feeding over 22 months. Treatments were composed of commercial formula feed (formula feed), TMR and Fermented-TMR (F-TMR). Intakes of dry matter and TDN were greater in the F-TMR than the other treatments during each growing and fattening phase (p<0.05). Although daily body gain of the F-TMR was lowest during the growing phase, but was 48% greater than those of other treatment during the finishing phase (p<0.05). Feed efficiency of the F-TMR was maintained constantly during whole growing and fattening phases. Carcass weight and rib-eye area of the F-TMR were greatest (p<0.05) among treatments. However, meat yield index and the rate of grade A in meat yield were highest in the formula feed having the thinnest back fat. Marbling score was hugely (p<0.05) increased when steers fed F-TMR, and therateofgrade 1+andoverinmeatqualitywas96%intheF-TMR. Although feed cost in the F-TMR was increased by 40% compare to the formula feed, but net income was increased by 29% due to improvement of meat quality and body gain.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.319-325
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• 2015

The safety of plant species used as a source for herbal medicines and dietary supplements has recently been questioned due to poisonings associated with the presence of cadmium (Cd) in these plants. These plants can derive Cd from their presence in the soil. Organic matter (OM) concentrations in soils could affect the availability of Cd for plants. To determine the effect of OM concentration in soil on the concentration of plant available Cd and uptake of this toxic element by medicinal plants, soil and plant samples were collected from 102 fields supporting for 5 species of medicinal plants in 6 province of South Korea. Concentrations of OM and dissolved organic carbon (DOC) in soils affected the phytoavailability of Cd. One M $NH_4OAc$ extractable Cd concentration in soil increased with increasing OM concentrations. There were significantly positive relationships between 1 M $NH_4OAc$ extractable Cd concentration and OM concentration in soil and between 1 M $NH_4OAc$ extractable Cd concentration and DOC concentration. Likewise, OM and DOC concentrations significantly affected Cd concentration in medicinal plant soils. Cadmium concentration in medicinal plants increased with increasing OM concentration in soil [Cd concentration $(mg\;kg^{-1})= 0.179+1.424{\times}10^{-3}$ OM concentrations, $R^2=0.042*$] and with DOC concentration [Cd concentration $(mg\;kg^{-1})= 0.150+5.870{\times}10^{-4}$ DOC concentrations, $R^2=0.124***$]. These results might result from Cd-DOC complex which is easily absorbed Cd form by plant root. Dissolved organic carbon concentration had more positive relationship with Cd concentration in medicinal plants and 1 M $NH_4OAc$ extractable Cd concentration in soils than OM. Cadmium concentration in all 5 species of medicinal plant (Atractylodes macrocephala Koidzumi, Astragalus membranaceus, Codonopsis lanceolata, Platycodon grandiflorum, and Rehmannia glutinosa) significantly increased with increasing DOC concentration in soil. From the above results, formation of Cd-DOC complex caused by OM application might be mainly attributed to increase in Cd concentration in medicinal plants.

• Journal of Life Science
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• v.28 no.11
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• pp.1285-1289
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• 2018

Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrates, isoflavones, and other nutrients for humans and animals. But, antinutritional factors in the raw mature soybean are exist. Kunitz trypsin inhibitor (KTI) protein and stachyose are main antinutritional factors in soybean seed. The genetic removal of the antinutritional factors will improve the nutritional value of soybean seed. The objective of this research was to breed a new yellow soybean strains (rs2rs2titi genotype) with the traits of lacking of KTI protein and low content of stachyose. Breeding population was developed from the cross of "Jinyangkong" and 15G1 parents. Presence or absence of KTI protein was detected based on Western Blot technique. Content of stachyose in mature seed was detected by HPLC. Total four new strains (603-1, 603-2, 625, and 694) with KTI protein free and low content of stachyose were selected. Four strains (603-1, 603-2, 625, and 694) have yellow seed coat and hilum. Plant height of 603-1 strain was 65 cm and 100-seed weight was 29.2 g. Plant height of 603-2 strain was 66 cm and 100-seed weight was 26.2 g. Plant height of 625 strain was 64 cm and 100-seed weight was 27.1 g. Content of stachyose for four new strains was 3.0~3.50 g/kg. Four strains selected in this research will be used to improve new yellow soybean cultivar with KTI protein free, and low content of stachyose.