• Journal of Food Hygiene and Safety
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• v.23 no.3
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• pp.239-247
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• 2008

Objective of this study was to investigate residual the levels of heavy metals in rice grain and soils of "Top-Rice" and common rice cultivation areas from 2005 to 2007. Soil and rice grain samples were taken from 33 "Top-rice" areas and neighboring paddies, and analyzed for the elements using ICP-OES and ICP-TOF-MS after acid digestion. A concentration of arsenic in paddy soil was 1.33 mg/kg which was below 1/5-1/11 fold of the threshold levels(concern: 4 mg/kg, action: 10 mg/kg), and paddy soil was 0.06 mg/kg of Cd(cadmium) being below 1/25-1/67 fold of the limits(concern: 1.5 mg/kg, action: 4 mg/kg). A level of Cu(copper) in paddy soil was 4.57 mg/kg which was below 1/11-1/27 fold of the threshold levels(concern: 50 mg/kg, action: 125 mg/kg), and Pb(lead) concentration in paddy soil was found to be a 4.68 mg/kg. In addition, Hg(mercury) concentration in paddy soil was to be a 0.03 mg/kg, which was below 1/131-1/328 fold of the threshold levels(concern: 4 mg/kg, action: 10 mg/kg). The average concentrations of As, Cd, Cu, Pb and Hg in the polished rice samples were 0.037, 0.043, 0.280, 0.048 and 0.002 mg/kg, respectively. These levels are lower than those of other countries in rice grains. Assuming the rice consumption of 205.7 g/day by total dietary supplements in Korea, the amount of total weekly metal intake of As, Cd, Cu, Pb and Hg by polished rice were estimated to be 0.0892, 1.035, 6.712, 1.161 and 0.054 ${\mu}g/kg$ body weigh/week, respectively. The PTWI(%) of As, Cd, Cu, Pb and Hg were 5.95(inorganic arsenic), 0.26(total arsenic), 14.79, 0.19, 4.65 and 1.07% estimated to be 0.0892, 1.035, 6.712, 1.161 and 0.054 ${\mu}g/kg$ body weigh/week, respectively. In conclusion, it was appeared that the heavy metals contamination in the brown and polished rice should not be worried in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.10
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• pp.1501-1505
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• 2008

A broiler experiment was conducted to compare the effects of duration and level of iron-methionine chelate (Fe-Met) supplementation on the iron, copper (Cu) and zinc (Zn) content of broiler meat. Two hundred and fifty hatched Ross broiler chickens were randomly assigned to 5 dietary treatments. Each treatment had 5 replicates of 10 birds (5 males and 5 females) each. Birds were housed in raised floor batteries and fed traditional broiler diets ad libitum for 5 weeks. Dietary treatments were as follows: Control and two levels of Fe-Met (100 or 200 ppm in Fe) supplemented for either the whole period (0-5 wk) or grower period (4-5 wk). Production performance was not significantly affected by treatments. Iron content in the muscles (breast, leg and wing) and organs (liver and spleen) were significantly (p<0.05) increased as the level and duration of Fe-Met supplementation increased. The highest concentration of iron was shown in Fe-Met 200 fed for the whole period. Liver contained the highest amount of iron followed by spleen, leg muscle, wing muscle and breast muscle. Supplementation of Fe-Met 200 for the grower period resulted in higher iron concentration in liver and spleen than supplementation of Fe-Met 100 for the whole period. However, the same treatment resulted in lower iron concentration in muscles (breast, leg and wing) than the treatment of Fe-Met 100 for the whole period. In order to achieve the highest iron enrichment in the muscles, Fe-Met should be supplemented at 200 ppm in Fe for the whole period (5 wks). Fe-Met supplementation increased copper concentration in all muscles and organs except wing muscle. Zinc concentration decreased in breast and wing muscle but tended to increase in leg muscle, liver and spleen by Fe-Met 200 supplementation. Color of muscle was not significantly affected by Fe-Met treatments. However, redness of leg and breast muscle, and yellowness of leg and breast muscle tended to increase by supplementation of Fe-Met for the whole period. It was concluded that iron content of broiler meat can be effectively enriched by supplementation of 200 ppm of Fe as Fe-Met for 5 wks.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1267-1274
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• 2018

Objective: The objective of the study was to investigate the effects of zinc amino acid complex (ZnAA) on growth performance, hematological and biochemical parameters in weanling pigs. Methods: In Exp. 1, a total of 216 Duroc${\times}$Landrace${\times}$Large White weanling pigs were assigned randomly to 6 dietary treatments. Each treatment had 6 replicates (pens) with 6 pigs each. The diets were corn-soybean meal based with supplementation of 0, 20, 40, 80, 120 mg Zn/kg from ZnAA or 40 mg Zn/kg from feed-grade zinc sulfate. The experiment lasted 42 days. In Exp. 2, a total of 180 weanling pigs were assigned randomly to 3 dietary treatments supplemented with 0, 80, or 800 mg Zn/kg from ZnAA. Results: In Exp. 1, pigs fed 40 to 80 mg Zn/kg from ZnAA had higher (p<0.05) average daily gain (ADG) than the unsupplemented group during d 0 to 14. During d 0 to 42, the pigs fed 20 to 120 mg Zn/kg from ZnAA had increased (p<0.05) ADG. Pigs fed 20 to 120 mg/kg Zn from ZnAA had lower feed:gain (p<0.05), increased the activity of serum Cu-Zn superoxide dismutase on d 14, and increased serum Zn levels on d 42 (p<0.05). In Exp. 2, pigs fed diets with 800 mg Zn/kg had increased average daily feed intake during d 15 to 28 (p<0.05) compared to the unsupplemented group. During d 0 to 28, the pigs fed supplemental Zn had increased ADG (p<0.05). On d 14 and d 28, pigs fed supplemental Zn had higher the serum alkaline phosphatase activities (p<0.05). No significant differences were observed in the hematological parameters and organ indices. Conclusion: Supplementation with 20 to 80 mg/kg Zn from ZnAA improved the growth performance in weaned pigs. The piglets can tolerate up to 800 mg/kg Zn from ZnAA with limited potential health effects.

• Journal of the East Asian Society of Dietary Life
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• v.20 no.6
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• pp.939-946
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• 2010

The purpose of this study was to enhance the quality of gochujang sauces containing Salicornia herbacea L. extracts at concentrations of 0%(C), 1%(RS-1), 2%(RS-2), and 3%(RS-3). Free sugars detected in gochujang sauce were sucrose, glucose, maltose, and fructose. pH level was not significantly different among the samples. As the contents of S. herbacea L. extracts increased, the salinity decreased. The colors of the gochujang sauce as indicated by -L, $a^{\ast\ast\ast}$, and $b^{\ast}$ values decreased as contents of gochujang sauce S. herbacea L. extracts increased. Most of the mineral contents of gochujang sauce containing S. herbacea L. extracts were higher than that of control. Overall acceptabilities of the S. herbacea L. extracts-added groups were in the order of 2%, 3%, 0%, and 1%. The 2% (RS-2) S. herbacea L. extract-added group was highly rated in general charateristics, making it the most desirable for making S. herbacea L. extract-added gochujang sauce.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.1
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• pp.17-26
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• 1999

This study was conducted to investigate the effect of Zn and Fe levels on lipid metabolism and micromineral contents in high fat diet-induced obese rats. Male sprague-dawley rats weighing approximately 100g were fed a normal or high fat diet. After 10 weeks high fat diet, rats were fed different levels of Zn and Fe. Serum triacyglycerol and HDL-cholesterol were affected to Zn and Fe levels in normal group. The micromineral (Zn, Fe, Cu, Mn) contents in liver were significantly different in high fat diet group by Zn intake levels and in normal diet group by Fe intake levels. However, micromineral contents in kidneys were not affected to different levels of Fe in both obese and normal rats. There were no differences in spleens of obese rats fed high fat diet according to Zn and Fe intake levels. But in normal group, Mn content of spleen were exclusively affected to Zn levels.

• Journal of the East Asian Society of Dietary Life
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• v.21 no.2
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• pp.215-221
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• 2011

The goal of this study was to evaluate the quality characteristics of various beans in distribution. The quality characteristics investigated were proximate composition, color, free sugars, organic acids, amino acids, and minerals. Bean samples analyzed were white soybeans (Glycine max. (L.) Merrill), kidney beans (Phaseolus vulgaris var. humilis), black soybeans (Glycine max (L.) Merr.), black small soybeans (Rhynchosia nulubilis), sword beans (Canavalia gladiata), and green beans (Phaseolus vulgaris). The highest contents of crude fat and crude protein were 17.60${\pm}$0.14% for white soybeans, and 42.38${\pm}$0.15% for black soybeans, respectively. Higher color values compared to the other samples were $L^*$ (64.07${\pm}$0.97) for sword beans, $a^*$ (15.64${\pm}$0.48) for kidney beans, and $b^*$ (22.92${\pm}$0.09) for white soybeans. The highest contents of sucrose, oxalic acid, and malic acid in black small soybeans were 54.23 mg/g, 23.26 mg/100 g and 18.24 mg/100 g, respectively. Xylose, galactose, lactose, malonic acid, succinic acid, and lactic acid were not detected in the soybeans studied, whereas the glutamic acid content of soybeans ranged from 2.68 to 6.18 g/100 g. Levels of K and Mg contents in soybean were higher than those of the other minerals.

• Journal of Nutrition and Health
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• v.32 no.8
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• pp.946-956
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• 1999

The iron nutritional status of 328(20-70 years old) women in Seoul and Puyo areas was evaluated using a dietary information and a measurement of hematological indices. The serum iron was measured by Red Blood Cell(RBC), Hemoglobin(Hb), Hematocrit(Hct), Serum Iron(S-Fe), Total Iron Binding Capacity (TIBC), Transferrin Saturation(TS) and Serum Ferritin(SF) and was analyzed with the information obtained by interviews which included socio-demographic variables and the dietary pattern of the subjects. The mean Hb was 12.6 $\pm$ 1.21g/dl(Seoul : 12.4 $\pm$ 1.14g/dl, Puyo : 13.2 $\pm$ 1.28g/dl), Hct was 38.7 $\pm$ 3.84%(Seoul: 37.5 $\pm$ 3.28%, Puyo: 4.19 $\pm$ 3.48%), S-Fe was 76.8 $\pm$ 31.49ug/dl(Seoul: 68.8 $\pm$ 27.3ug/dl, Puyo: 78.8 $\pm$ 32.3ug/dl) and TIBC was 277.7 $\pm$ 86.15ug/dl(Seoul: 354.1 $\pm$ 129.8ug/dl, Puyo: 259.0 $\pm$ 59.55ug/dl). The mean Ts(%) was 30.9 $\pm$ 17.9% (Seoul: 21.7 $\pm$ 10.52%, Puyo: 33.2 $\pm$ 18.68%) and the level was significantly higher in Puyo women(p<0.05). The mean SF was 45.4 $\pm$ 46.21ng/ml(Seoul: 53.6 $\pm$ 50.21ng/ml, Puyo: 36.1 $\pm$ 39.83ng/ml). The Red Blood Cell(RBC) showed a negative correlation with age and Hb, Hct showed a negative correlation with education and income level. However TIBC showed a and total energy expenditure per day. Energy, protein, carbohydrate, iron and vit.C intake showed a positive correlation with Hb and Hct. In particular, heme iron showed a positive correlation with RBC and nonheme iron of Hb, income level and iron intake affected on the level of Hct. In Seoul women, the age of menarche affected on the level of TIBC, total energy expenditure affected on the level of Hct. In Seoul women, the age of menarche affected on the level of TIBC, total energy expenditure affected on the level of S-Fe.

• Journal of Life Science
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• v.19 no.9
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• pp.1258-1264
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• 2009

In this study, effects of feeding Holstein cows with TMR feed, which includes un-dried citrus byproducts, on the nutritional components of their raw milk were investigated. Two kinds of raw milk from primipara cows and multipara cows were used for the experiment: T0 (raw milk produced by dairy cows not fed with TMR feed including citrus byproducts) and T1 (raw milk produced by dairy cows fed with TMR feed including citrus byproducts). For both primipara and multipara cows, there was no statistically meaningful difference between T0 and T1 in terms of moisture, crude protein, crude fat, crude ash contents or caloric content. T1 showed a lower amount of cholesterol in raw milk for both primipara and multipara cows, which was especially statistically meaningful in multipara cows (p<0.05). Regarding mineral contents in raw milk, T1 showed significantly higher amounts of Na and S in primipara cows, and Na, Cu, Fe, S in multipara cows, respectively. For both primipara and multipara cows, there were no statistically meaningful differences between T0 and T1 in terms of the total amount of amino acids in the raw milk, the composition of saturated fatty acid/unsaturated fatty acid, vitamin A, E, $B_2$ or $\beta$-carotene, while T1 showed significantly higher levels of vitamin $B_1$ in primipara cows than T0.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.74-75
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• 2005

In order to evaluate dietary brown seaweed and vancomycin on the performance, layers(Isa brown) were fed on basal diet and diets containing 2.0% of brown seaweed or 10ppm vancomycin. Brown seaweed diet significantly increased(p<0.01) nitrogen balance in layer, while excretion of uric acid nitrogen and metabolizable energy utilization were not different among diets. Layer consumed more the brown seaweed diet(p<0.05). Egg production were significantly different by diets but reduced(p<0.0001) with the experimental period passed. Layer fed brown seaweed diet gave thicker shell eggs, higher Haugh unit and higher egg white CuZnSOD activity compared with those in basal diet. Also, Brown seaweed diet increased MnSOD activity in erythrocyte cytosol and peroxidase in plasma, but decreased peroxide level in plasma, and increased proliferation of PBMC stimulated with PHA-P The result indicated that brown seaweed 2.0% diet in layer improved egg quality and performance due to increased protein synthesis which were related to regulation of antioxidant system and immune cell function in blood.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.203-208
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• 1999

An 8 week feeding trial was conducted with 864 ISA Brown laying hens, 48 weeks old, to determine if microbial phytase $(Natuphos^{(R)})$ supplementation can reduce non-phytate phosphorus (NPP) level in laying diets. The experiment consisted of four dietary treatments: T1, control diet with 0.26% NPP (0.55% total P) wand no supplementary phytase; T2, 0.21% NPP (0.50% total P) diet with 250 U of phytase/kg of diet; T3, 0.16% NPP (0.45% total P) diet with 250 U of phytase/kg of diet; and T4, 0.11% NPP (0.40% total P) diet with 250 U of phytase/kg of diet. T3 showed the highest egg production and egg weight and the lowest feed conversion while T4 gave the lowest egg production and the highest feed conversion and mortality. Daily feed consumption ranged from 130.4 g (T4) to 132.7 g (T2). T1 and T2 were not significantly different in the production parameters. Eggshell strength, egg specific gravity, and eggshell thickness were not significantly different among treatments. However, broken egg ratio was significantly lower in T2 and T4 than in T1. Retentions of Ca, P, Mg, and Cu were greater in phytase supplemented treatments (T2, T3, and T4) than the control (T1), and those in T3 and T4 were greater than in T2. Excretions of P in phytase supplemented treatments (T2, T3, and T4) were significantly (p<0.05) smaller than in T1 but excretions of N were not significantly different among the treatments. Contents of ash in tibiae were not significantly affected by treatments, but contents of Ca, P, Mg, and Zn was increased and that of Cu decreased by phytase supplementation. It is concluded that the NPP concentration in the diet of Brown layers consuming about 130 g/d of feed can be safely lowered from 0.26% (0.55% total P) to 0.16% (0.45% total P). The excretion of P was reduced by the inclusion of 250 U phytase/kg of diet.