• Journal of the Korean Wood Science and Technology
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• 제42권2호
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• pp.207-213
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• 2014

This study was carried out to investigate the feasibility and usefulness of a commercial synthetic adsorbent, Diaion HP 20, for the isolation of proanthocyanidins (PAs) from hot water extract (HWE) prepared from Pinus radiata bark. Most ultraviolet (UV) absorbing materials in HWE were adsorbed onto Diaion HP 20 and easily recovered by simple ethanol (EtOH) washing. More than 50% of the UV absorbing materials were adsorbed within 20 minutes, and the adsorption equilibrium was reached within 3 h. The recovered materials from Diaion HP 20 were mostly composed of PAs, but some monomeric flavonoids such as taxifolin and unidentified hydrophobic materials were also adsorbed. The impurities such as carbohydrates and inorganic materials contained in HWE were mostly removed by Diaion HP 20. The adsorption ability of the UV absorbing materials onto Diaion HP 20 was almost the same as the first cycle even after 14 times of repeating cycles of adsorption and desorption. The radical scavenging ability of the recovered materials from the adsorbent was slightly higher than that of the pure PAs prepared by Sephadex LH 20 from the same HWE.

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.201-204
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• 2003

Glycopeptide antibiotics, teicoplanin was purified from a mutant strain of Actinoplanes teichomyceticus ATCC31121, A. teichomyceticus MSL2211. We developed a simple procedure to separate and purify the teicoplanin from the fermentation broth. Teicoplanin was purified by two-step purification system, hydrophobic adsorption and sugar affinity chromatography in combination with HPLC analysis based on the properties of hydrophobic acyl chain and sugar moiety in teicoplanin. Teicoplanin was separated from the culture broth by Diaion HP-20 and further purified by concanavalin A affinity column chromatography. As an adsorbent resin, Diaion HP-20 in broth eliminated toxic effects on growth, reduced feedback repression of teicoplanin production, and assisted In rapid recovery of teicoplanin. The teicoplanin displayed the final yield of 80% and 95% of purity.

• Journal of Ginseng Research
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• 제22권3호
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• pp.155-160
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• 1998

This stuffy was carried to establish a new efficient method for the preparation of edible crude ginseng saponin. The conventional butanol extraction and resin adsorption methods were compared for the contents of total crude ginseng saponin and major ginsenosides. Seventy- percent methanol extract was applied to Diaion HP-20 column and the resin was washed with Hn and eluted with absolute methanol. The methanol elute was dried in vivo and analyzed for its ginsenosides. Use of ethanol instead of methanol to make edible crude ginseng saponin gave a similar result. Butanol extraction was performed by the conventional method. The final aqueous layer from butanol extraction was passed through Diaion HP-20 column followed by elution with methanol and Diaion HP-20 passed fraction was extracted with butanol to recover remaining components, respectively, in order to determine saponin loss. TLC and HPLC qualitatively and quantitatively monitored Ginsenosides, respectively. Loss of ginsenosides was higher in butanol extraction method than in Diction HP-20 adsorption method. In addition, saponin fractions prepared by Diction HP-20 adsorption method showed higher content of each ginsenoside, showing 8.2% higher purity than that of butanol extracted fraction. From these results, we propose the resin adsorption method as a new efficient measure for the preparation of crude ginseng saponin, which is edible by using spirit instead of methanol.

• 한국약용작물학회지
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• 제28권1호
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• pp.29-37
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• 2020

Background: Although Gastrodia elata contains the valuable, bio-active compounds gastrodin and 4-hydroxybenzyl alcohol (4-HBA), it also contain ρ-cresol, which is a major source of an objectionable pig slurry-like odor. Accordingly, this study was performed to determine the deodorization effect of Diaion HP-20 resin on a G. elata extract. Methods and Results: Using Diaion HP-20 with an increasing concentration of ethanol, an extract of G. elata was fractionated into 15 fractions, which were anlayzed to determine their concentrations of active compounds and ρ-cresol. The gastrodin, 4-HBA, total phenolics, and ρ-cresol concentrations of the G. elata extract were 403.13, 408.15, 2,476.09, and 11.77 ㎍/㎖, respectively. Among the different fractions, gastrodin and 4-HBA were detected in fractions 3 to- 6, and 4 to- 10, respectively whereas total phenolics were detected in all samples, with fractions 5 to- 7 being characterized by higher concentrations than were the other fractions. ρ-Cresol was detected in fractions 12 to- 15, at concentrations ranging from 0.39 ㎍/㎖ to 6.05 ㎍/㎖ with the highest concentration detected in fraction 14. The fractions containing ρ-cresol could be selectively separated from the G. elata extract without the loss of major bio-active compounds such as gastrodin and 4-HBA. Conclusions: The results of this study indicate that a deodorizing method using Diaion HP-20 resin can be considered for improving the sensory qualities of G. elata extract without a loss of the major bio-active compounds.

• 한국자원식물학회지
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• 제27권6호
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• pp.635-641
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• 2014

Scutellaria baicalensis $G_{EORGI}$ (Scutellariae Radix) has been used to clear heat and to dry dampness in the stomach or intestines, which manifests as diarrhea or dysenteric disorder. In this study, we investigated the effective preparation of active components in Scutellariae Radix using the methods of solvent extraction and absorption fractionation for the development of new functional food or pharmaceuticals. The marker substances, baicalin, baicalein, wogonoside, and wogonin were directly isolated from the Scutellariae Radix. There chemical structures were elucidated by spectroscopic analysis. The Scutellariae Radix was extracted with hot water. To enhance yield of flavonoids in Scutellariae Radix, the hot water extract was dissolved in ethanol with concentration dependent manner. The precipitates were separated using centrifugal techniques at 10,000 rpm. Supernatant liquid was applied to the HPLC for quantification of major compounds. Separately, the hot water extract was absorbed on Diaion HP-20 resin. And then, the absorbed fraction was eluted with methanol for HPLC. The contents of baicalin, baicalein, wogonoside and wogonin in different treatment methods were analyzed by HPLC. Total amount of four major components were 16.9% in 50% ethanol extract, 21.7% in 70% ethanol extract, 20.5% in 90% ethanol extract, and 39.3% in absorbed fraction of Diaion HP-20 resin. In these results, we found that resin absorption method is suitable for the extraction of enriched flavonoids from Scutellariae Radix.

• 한국식품영양과학회지
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• 제30권1호
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• pp.1-5
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• 2001

This study was carried out to isolate saponin compounds from red-ginseng efflux, which was produced during the industrial processing of red-ginseng from fresh ginseng. We isolated crude saponin from the efflux extract (moisture content 35.0%) by using Diaion HP-20 adsorption method. Non-saponin fraction, which was adsorbed on Diaion HP-20 resin, was removed by eluating with $H_{2}O$ and 25% spirit. Then crude saponin was eluated with 95% spirit, continuously. Saponin in the eluated fractions was confirmed by TLC analysis. Crude saponin isolated from red ginseng efflux extract contained 12.10% of saponin. whereas those of white ginseng and red-ginseng were 3.30 and 3.39%, respectively. Ginsenoside contents showed the highest contents kin crude saponin from red ginseng efflux extract. Expacilly, the ginsenoside-$Rb_{1}$ and Re showed the highest contents in red-ginseng efflux extract when compared with those of white ginseng and red ginseng crude saponins. And the other ginsenosides except ginsenoside-$Rb_{1}$ and -Re also showed the highest contents in red ginseng efflux extract. However, the ratio of PD saponin (Panaxadiol saponin: $Rb_{1}+Rb_{2}$+Rc+Rd) to PT saponin (panaxatriol: $Re+Rg_{1}$) showed almost the same level when compared with those of ginseng saponin fractions. Ratio of PD/PT from red ginseng efflux extract was 1.99. Ratios of PD/PT from white ginseng and red ginseng were 1.85 and 1.84, respectively. Saponin purity, which was calculated by ratio percent of total ginsenoside to curde saponin content, was 45.90%. In case of white ginseng and red ginseng, the purities were 35.50 and 36.00%, respectively. However, by PHLC analysis, we confirmed that crude saponin isolated from red ginsengs. It suggested that crude saponin isolated from red ginseng ellux also would be useful component as ginseng saponins.

• Journal of Microbiology and Biotechnology
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• 제29권6호
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• pp.897-904
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• 2019

Monascus purpureus recombinant mppC and mpp7 knockout strains were subjected to extractive fermentation in the context of azaphilone pigment production. Inclusion of Diaion HP-20 resin resulted in the selective production of unreduced azaphilone congeners, in addition to the early intermediate FK17-P2a, from ${\Delta}mppC$ and ${\Delta}mpp7$ strains that would otherwise mainly produce reduced congeners. Structural determination of two novel unreduced azaphilones from the ${\Delta}mpp7$ strain was accomplished. The unreduced azaphilone compound was converted into the cognate reduced congener in recombinant M. purpureus strains, demonstrating its intermediate role in azaphilone biosynthesis. This study demonstrates the possibility that extractive fermentation with Diaion HP-20 resin can be used to obtain cryptic azaphilone metabolites.

• 한국식품과학회지
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• 제43권2호
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• pp.149-155
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• 2011

4종류의 다공성 합성흡착제(Sepabeads 207, 700, 850, Diaion HP 20)를 사용하여 Rubus coreanus 과실의 물 추출물로부터 과당 및 비polyphenol성분을 효과적으로 제거하여 DPPH라디칼 소거능 및 화학발광법을 이용한 superoxide 라디칼 소거능이 증가된 농축물을 효율적으로 얻을 수 있었다. 또한, 실시간 HPLC $ABTS^{{\cdot}+}$ 활성분석 시스템을 활용하여 6개의 항산화 능력을 갖는 활성피크를 확인할 수 있었으며 이들 대상으로 LC-MS/MS분석을 실시하여 5개의 anthocyanin인 cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside, pelargonidin-3-rutinoside를 mass pattern 및 분자량 확인 과정을 통해 잠정적 화학구조 동정을 하였다. 수율과 항산화 효능을 고려해 볼 때, 4종의 합성흡착제 중에서 Diaion HP 20이 가장 우수한 결과를 나타내었다. 이처럼 적절한 합성흡착제의 선택으로 표적물질에 대한 polyphenol성 물질의 농축뿐만 아니라 생리학적 활성증대효과를 가져올 수 있어 적절한 합성흡착제의 선택은 식품 및 의약품의 정제 및 분리에 유용하게 활용될 수 있음이 확인되었다.

• Journal of Applied Biological Chemistry
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• 제61권4호
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• pp.327-334
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• 2018

Monascus is a source of food colorant with high productivity of the pigment azaphilone. Monascus azaphilone (MAz) is biosynthesized through a single non-reducing polyketide pathway, the major components of which are ankaflavin (1), monascin (2), rubropunctatin (3) and monascorubrin (4); valuable biological activities have been reported for these compounds. Thus, various culture conditions were explored to reduce the cost of culture ingredients, enhance productivity and modulate compound composition. In the present study, we examined an extractive fermentation (EF) method with Diaion HP-20 resin (HP20) in direct comparison to a previously explored method involving Triton X-100 (TX100) to explore the modulated production of the major MAzs. We employed wild-type Monascus purpureus as well as two derivative recombinant strains (${\Delta}mppG$ and ${\Delta}mppE$) that are known to have differential MAz profiles as that of the wild-type strain. The HP20 resin was capable of modulating the MAz profile in favor of orange MAzs 3 and 4, oxidized congeners in this class, as was TX100-a phenomenon not previously observed for TX100 EF with Monascus anka. These finding substantiate that HP20 can be employed for the selective production of oxidized MAz and for diversifying the culture conditions used for Az production.

• 대한화학회지
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• 제27권5호
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• pp.345-352
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• 1983

Diphenylcarbazide를 흡착시킨 큰 그물구조 수지를 사용하여 컬럼 조작에 의해 0.1M 황산수용액에서 크롬(Ⅵ)이온을 선택적으로 농축하였다. 시료수의 액성과 유속을 조절하여 각종 수지에 대해 크롬(Ⅵ)이온 추출실험을 한 결과 Diaion HP-20이 가장 큰 돌파교환용량을 나타내었다. Diaion HP-20에 대해 크롬(Ⅵ)이온의 돌파점은 흡착된 DPC의 농도에 비례하였으며 크롬(Ⅵ)이온의 농도가 진할 때가 묽을 때 보다 높은 돌파교환용량을 나타냈다. 또한 일반 환경수 속에 공존되기 쉬운 각종 중금속 이온들에 대한 크롬(Ⅵ)이온의 농축에 미치는 영향을 조사한 결과 10ppm 크롬(Ⅵ)이온에 대해 철(Ⅲ)이온을 제외한 모든 금속 이온들은 100배까지 공존하여도 영향을 미치지 않았으며 철(Ⅲ)이온은 10배까지 방해되지 않았다. 크롬(Ⅵ)이온 추출에 이용된 겔 입자는 메탄올로서 쉽고도 효과적으로 재생되므로 반복 사용할 수 있다.