Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Polyaromatic Resin HP-20 Induced Accumulation of Intermediate Azaphilones in Monascus purpureus 𝚫mppC and 𝚫mpp7 Strains

  • Lim, Yoon Ji (Department of Biological Sciences and Bioinformatics, Myongji University) ;
  • Lee, Doh Won (Department of Biological Sciences and Bioinformatics, Myongji University) ;
  • Choi, Jeong Ju (Department of Biological Sciences and Bioinformatics, Myongji University) ;
  • Park, Si-Hyung (Department of Oriental Medicine Resources and Institute for Traditional Korean Medicine Industry, Mokpo National University) ;
  • Kwon, Hyung-Jin (Department of Biological Sciences and Bioinformatics, Myongji University)
  • Received : 2019.02.22
  • Accepted : 2019.05.13
  • Published : 2019.06.28
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Abstract

Monascus purpureus recombinant mppC and mpp7 knockout strains were subjected to extractive fermentation in the context of azaphilone pigment production. Inclusion of Diaion HP-20 resin resulted in the selective production of unreduced azaphilone congeners, in addition to the early intermediate FK17-P2a, from ${\Delta}mppC$ and ${\Delta}mpp7$ strains that would otherwise mainly produce reduced congeners. Structural determination of two novel unreduced azaphilones from the ${\Delta}mpp7$ strain was accomplished. The unreduced azaphilone compound was converted into the cognate reduced congener in recombinant M. purpureus strains, demonstrating its intermediate role in azaphilone biosynthesis. This study demonstrates the possibility that extractive fermentation with Diaion HP-20 resin can be used to obtain cryptic azaphilone metabolites.

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