• Development and Reproduction
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• 제20권3호
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• pp.247-254
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• 2016

Cytological changes of the epithelial cells according to the developmenatal phases of the seminal vesicle related to the spermatogenic stages in the testicular lobules during spermagenesis in male Neptunea (Barbitonia) cumingii (Gastropoda: Buccinidae) were investigated monthly by electron microscopical and histological observations. N. (B) cumingii is dioecious, and an internal fertilization species. The male genital organ is located near the tentacles. The spermatozoon is approximatley $50{\mu}m$ in length. The axoneme of the tail flagellum consists of nine pairs of microtubles at the periphery and one pair at the center. The process of germ cell development during spermatogenesis can be divided into five succesive stages: (1) spermatogonia, (2) primary spermatocytes, (3) secondary spermatocytes, (4) spermatids, and (5) spermatozoa. A considerable amount of spermatozoa make their appearance in the testicular lobules (or acini) and some of them are tranported from the testis towards the seminal vesicles until late July. In this study, the developmental phases of the epithelial cells of the seminal vesicles of N. (B.) cumingii could be classified into four phases: (1) S-I phase (resting), (2) S-IIphase (early accumulating), (3) S-III phase (accumulating), and (4) S-IV phase (spent). However, in case of N. (B.) arthritica cumingii, the developmental phases of the seminal vesicle were devided into three phases: (1) resting, (2) accumulating and (3) spent. Granular bodies in the inner layer of the seminal vesicles are involved in resorption of digestion of residual spermatozoa.

• Development and Reproduction
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• 제19권1호
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• pp.33-41
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• 2015

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-$17{\beta}$ of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-$17{\beta}$ were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

• Korean Journal of Animal Reproduction
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• 제19권4호
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• pp.265-270
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• 1996

This study was carried out to investigate on the survival rates and in vitro developmental rates of bisected bovine embryos by micromanipulator and micropipette. Bisected embryos were cultured for 1∼5 days in 20% FCS＋TCM-199 medium. Survival rate and in vitro developmental rate were defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The survival rates of intact or free zona pellucida of bisected embryos were 30.3 and 25.0%, respectively. And the survival rates of bisected embryos by micromanipulator and micropipette were 33.3 and 26.7%, respectively. The survival rate of bisected embryos was significantly lower than that of non-bisection embryos(65.0%). 2. The survival rates of bisection embryos in cultured for 12, 24, 48, 72 hrs with 20% FCS＋TCM-199 medium were 40.0, 30.0, 23.3 and 13.3%, respectively. 3. The in vitro developmental rates of intact of free zona pellucida of bisected embryos by micromainipulator and micropipettes were 33.3 and 26.7%, respectively. The survival rate of bisection embryos was significantly lower than that of non-bisection embryos(45.0%).

• Korean Journal of Environmental Biology
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• 제17권4호
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• pp.401-405
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• 1999

Comprehensive collecting survey were performed fer study on ovipositioning habits and developmental process of Rana temporaria dybowskii from mid February 1998 to late May 1998 from Kyunggi-do, Kangwon-do and other areas in South Korea. Oviposition occurred in both running water and pool between late February and late May. In lotic habitat, they laid egg mass in irregularly ball shaped, and eggs were sticked to substrates such as stone or macrophytes. On contrary, in the pool, they laid an oval shaped egg mass not sticked to any other substrates. Every egg was hatched at day 7, and all developmental processes were completed at days 71 after oviposition. Developmental process of this species was relatively short, and this may increase predation activities during abundant prey season.

• Toxicological Research
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• 제19권2호
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• pp.123-131
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• 2003

This study was carried out to assess the combined repeated dose, reproduction and developmental toxicities of benzoyl peroxide for OECD SIDS (Screening Information Data Set) program. Male and female Sprague-Dawley rats were exposed to benzoyl peroxide at dose levels of 0, 250, 500 and 1,000 mg/kg/day for 29 days for males and for 41-51 days for females. No deaths were found in all animals including control group during exposure period. No hematological effects attributable to benzoyl peroxide were observed in all treated groups. Significant decrease in the weight of testes and epididymis were observed in males at 1,000 mg/kg/day. In females at 1,000 mg/kg/day, slight histopathological effects in uterus such as epithelial vacuolation or hyperplasia were observed. No treatment-related changes in precoital time and rate of copulation, fertility and gestation period were noted in all treated groups. There was no evidence of teratogenic effect of benzoyl peroxide, but body weight of pups at 1,000 mg/kg/day was significantly decreased. NOAEL for combined repeated dose and reproduction/developmental toxicity was 500 mg/kg/day.

• Korean Journal of Social Welfare
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• 제48권
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• pp.179-213
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• 2002

The purpose of this study is to examine the effect solution-focused brief group therapy program on a relationship between developmental disorder children and their parents. The following research question is formulated to address the purpose: (1) Does solution-focused brief group therapy program will improve interaction on parents's "warmth" and "involvement"? (2) Does solution-focused brief group therapy program will improve interaction on child's overall action and relative behavior? (3) Does solution-focused brief group therapy program will improve relationship between the developmental disorder children and their parents? For research, 12 disabled children and their parents are assigned into two groups: Experimental and Control group. Findings are that. (1) The solution-focused brief group therapy program showed positive reaction on "parent's warm heart" but there was no reaction on "involvement". (2) The solution-focused brief group therapy program affect on interaction types of the disabled children. 3) According to self-recorded report, the parents' satisfaction of consultation has been increased by frequency. These results showing that the solution-focused brief group therapy are affected the interaction between developmental disorder and their own parents. Clinically, this outcome shows a significant practical implications on social work intervention.

• Human Ecology Research
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• 제52권2호
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• pp.189-198
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• 2014

The purpose of this study was to examine the effects of the We Start center visiting intervention program on the positive changes in the developmental areas and literacy interests of young children from low-income families. The subjects were 195 young children (109 in the experimental group, M=71.7 months; 86 in the control group, M=73.3 months) living in We Start areas (Ansan, Gwangmyeong, and Suwon cities) of Gyeonggi-do. The We Start center visiting intervention programs were conducted for 30-34 weeks in each city, and pre-tests and post-tests were conducted before and after the intervention programs. The instruments used were the developmental checklist and the literacy interests test The developmental checklist consisted of 7 domains (personality & sociality; language, reading, & writing; scientific thinking; mathematical thinking; arts; social learning; and physical development). The literacy interests test consisted of 2 factors (interest in literacy and interaction during activity). The scores on the developmental checklist showed positive changes in several domains (personality & social development; language, reading, & writing ability; scientific thinking; and social learning), but not in mathematical thinking, arts, and physical development. Second, the results of the literacy interests test showed positive effects on interaction during activity and the total score. In conclusion, the We Start center visiting program for young children from low-income families is an effective early intervention program to end the intergenerational transference of poverty in Korea.

• International Journal of Oral Biology
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• 제30권4호
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• pp.135-141
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• 2005

Although it has been known that TGF-${\beta}1$ acts as a crucial cofactor in osteoclast differentiation, its mode of action is still unclear. In the present study, we studied the effect of TGF-${\beta}1$ on the differentiation of osteoclast depending on the developmental stages. Murine bone marrow cells were induced to differentiate into mature osteoclasts in the presence of receptor activator of NF-${\kappa}B$ ligand (RANKL) and macrophage colony stimulating factor (M-CSF). In the early stage of the differentiation TRAP(-) mononuclear precursor cells were obtained from nonadherent M-CSF dependent bone marrow cells, which further differentiated into mature osteoclasts. TGF-${\beta}1$ stimulated osteoclast differentiation, which was stronger when cells were stimulated by TGF-${\beta}1$ in the early stage than the later differentiation. TGF-${\beta}1$ increased the expression of RANK and synergistically stimulated RANKL-induced activation of NF-${\kappa}B$ MAP kinase in TRAP(-) mononuclear precursor cells. These results suggest that activation of osteoclast differentiation by TGF-${\beta}1$ may be ascribed to the both increased expression and activation of RANK in the osteoclast differentiation, especially in the early stage of differentiation.

• Animal cells and systems
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• 제3권1호
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• pp.97-102
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• 1999

In order to investigate the regional distribution and relative frequency of the serotonin-, somatostatin- and motilin-immunoreactive cells in the gastrointestinal tract GIT) of the bullfrog (Rana catesbeiana) with developmental stages, group of bullfrogs subdivided into the tadpole with hindlegs, metamorphosed bullfrog with tail, 2 wk after metamorphosed bullfrog and adult bullfrog, were stained by immunohistochemical methods (PAP methods). Multifarious changes of the regional distribution and relative frequency of the gastrointestinal endocrine cells were observed as developmental stages but motilin-immunoreactive cell ere not detected in this study. Serotonin-immunoreactive cells were detected from 2 wk after metamorphosed bullfrog in the whole GIT. The positive cells were predominant in the pylorus regions. In the adult bullfrog, these cells increased in fundus. On the other hand, somatostatin-immunoreactive cells were observed from the tadpole with hindlegs in the stomach regions. In the metamorphosed bullfrog with tail, they were found in the whole GIT except for the ileum, and increased with developmental stages except for rectum. In the rectum, these cells were not detected after that period. Our results show that most of the immunoreactive cells appear from completed metamorphosed periods when the histological structure of GIT is completely differentiated.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제3권1호
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• pp.138-146
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• 1992

The study presented the experience of playtherapy of a 6-yr old girl with elective mutism and attempted to understand her psychopathology and formulated her psychodynamic which was expressed though the therapeutic process of playtherapy in the viewpoint of developmental model. Therapist's attitude was nondirective and supportive. Main theme of her play, fantasy and art was separation anxiety and abandonment fear as the result of continued psychic traumas such as separation from her grandmother and physical abuse by her father and mother etc. So she couldn't achieve developmental tasks such as autonomy and separation individuation and became generally inhibited and selectively mute. The process of play therapy was summarized and coceptualized as her successful progress through sequential developmental phases within the therapeutic relation ship. By reexperiencing this developmental process, she could accomplish her autonomy and separation individuation and developed to oedipal stage and successfully resolve oedipal conflict.