• BMB Reports
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• v.38 no.4
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• pp.500-506
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• 2005

HOX11 encodes a homeodomain-containing transcription factor which directs the development of the spleen during embryogenesis. While HOX11 expression is normally silenced through an unknown mechanism in all tissues by adulthood, the deregulation of HOX11 expression is associated with leukemia, such as T-cell acute lymphoblastic leukemia. The elucidation of regulatory elements contributing to the molecular mechanism underlying the regulation of HOX11 gene expression is of great importance. Previous reports of HOX11 regulatory elements mainly focused on the 5'-flanking region of HOX11 on the chromosome related to transcriptional control. To expand the search of putative cis-elements involved in HOX11 regulation at the post-transcriptional level, we analyzed HOX11 mRNA 3'-untranslated region (3'UTR) and found an AU-rich region. To characterize this AU-rich region, in vitro analysis of HOX11 mRNA 3'UTR was performed with human RNA-binding protein HuR, which interacts with AU-rich element (ARE) existing in the 3'UTR of many growth factors' and cytokines' mRNAs. Our results showed that the HOX11 mRNA 3'UTR can specifically bind with human HuR protein in vitro. This specific binding could be competed effectively by typical ARE containing RNA. After the deletion of the AU-rich region present in the HOX11 mRNA 3'UTR, the interaction of HOX11 mRNA 3'UTR with HuR protein was abolished. These findings suggest that HOX11 mRNA 3'UTR contains cis-acting element which shares similarity in the action pattern with RE-HuR interactions and may involve in the post-transcriptional regulation of the HOX11 gene.

• BMB Reports
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• v.49 no.10
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• pp.554-559
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• 2016

Mycobacterium abscessus, a member of the group of non-tuberculous mycobacteria, has been identified as an emerging pulmonary pathogen in humans. However, little is known about the protective immune response of antigen-presenting cells, such as dendritic cells (DCs), which guard against M. abscessus infection. The M. abscessus gene MAB1843 encodes ᴅ-alanyl-ᴅ-alanine dipeptidase, which catalyzes the hydrolysis of ᴅ-alanyl-ᴅ-alanine dipeptide. We investigated whether MAB1843 is able to interact with DCs to enhance the effectiveness of the host's immune response. MAB1843 was found to induce DC maturation via toll-like receptor 4 and its downstream signaling pathways, such as the mitogen-activated protein kinase and nuclear factor kappa B pathways. In addition, MAB1843-treated DCs stimulated the proliferation of T cells and promoted Th1 polarization. Our results indicate that MAB1843 could potentially regulate the immune response to M. abscessus, making it important in the development of an effective vaccine against this mycobacterium.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.2
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• pp.397-405
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• 2015

In the past, expressways focused on mobility. However, the paradigm of expressways fuction today has been changed from fast expressways to safe expressways as people's quality of living and consciousness level heightened. In 2012, 3,550 traffic accidents occurred on expressways and 371 people died. The fatality rate of traffic accidents on expressways is almost twice that on general national roads. This study developed accident forecast models (safety performance functions) based on the number of traffic accidents and traffic volumes on six major lines on expressways. It is difficult to forecast safety performance functions for each expressway line because the lines and the scales of expressways are different from each other; therefore, integrated safety performance functions of six lines were determined first, and the coefficients, which can correct the traffic accidents on each line, were calculated. It is believed that this study will contribute in the safer management of expressways by being used as basic information in the establishment of traffic safety strategies for each expressway line in prevention of traffic accidents. Moreover, more studies would be required in the future, which would suggest reliable accident forecasts by calculating correction coefficients by line through integrated models by groups dependent on the characteristics of each line.

• Journal of Advanced Marine Engineering and Technology
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• v.11 no.2
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• pp.71-81
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• 1987

Nowadays, the high development of industrial technique demands the optimal design of marine structures to be welded under the water, because the underwater welding of the ship hull and marine structures can decrease manpower and cost of production. However there is not available at present any report on fatigue behavior about underwater welded joints. In this paper under tention- tension repeated fatigue stress with frequency of 10 cycles per second by local controlled system, the fatigue strength properties of underwater welded joints of SM41A-2 Plate-to-Plate of 10 mm thickness were experimentally examined. The results obtained were as follows : 1) The fatigue strength of underwater welded joints of SM41A-2 was peaked at the heat input of about 1, 400 joule/mm(180 A, 36 V), while, at the heat input of more than about 1, 100 joule/mm (160 A, 33 V) that of the underwater welds at the higher than cycle of life rather than the lower cycle was higher than that of the base metal but lower than that of the atmosphere welds on account of both cooling and notch effects. 2) The fatigue limit of underwater welds increased with an increase of heat input resulting in a peak of that at the heat input of about 1, 400 joule/mm and then decreased gradually. 3) The fatigue strength at N cycles was peaked between the heat input of about 1, 400 and 1, 700 joule/mm where the strain was rapidly increased. 4) It was confirmed that the optimal zone of heat input condition for obtaining the underwater welds fatigue strength higher than that of the base metal exists, and if out of this zone, the fatigue strength of the underwater welds was lower than that of the base metal because of lack weld penetration, inclusion of slag, voids, etc. 5) By the fatigue test, the underwater welds fractured brittly without visual deformation, so the strain was remarkably less than of the atmosphere welds. 6) The fatigue life factor was peaked at the heat input of about 1, 600 joule/mm (200 A, 36 V) at which the mean strain is a little higher than that of the base metal but quite lower than those of the atmosphere welds, resulting in good underwater welds because both fatigue strength and ductility of the underwater welds are higher than those of the base metal at such heat input.

• Progress in Medical Physics
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• v.24 no.3
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• pp.191-197
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• 2013

Upon radiation treatment, it is the important factor to monitor the patient's motion during radiation irradiated, since it can determine whether the treatment is successful. Thus, we have developed the system in which the patient's motion is monitored in real time and moving treatment position can be automatically corrected during radiation irradiation. We have developed the patient's position monitoring system in which the patient's position is three dimensionally identified by using two CCD cameras which are orthogonal located around the isocenter. This system uses the image pattern matching technique using a normalized cross-correlation method. We have developed the system in which trigger signal for beam on and off is generated by quantitatively analyzing the changes in a treatment position through delivery of the images taken from CCD cameras to the computer and the motor of moving couch can be controlled. This system was able to automatically correct a patient's position with the resolution of 0.5 mm or less.

• Journal of the Korean School Mathematics Society
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• v.17 no.4
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• pp.771-804
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• 2014

The concept of the center of gravity is presently being introduced in elementary school curriculums and is broadly applied to Mathematics, Physics, and the Engineering field in University education which are mostly theoretical classes much separated from actual life in the practical educational field. In 2013, ${\bigcirc}{\bigcirc}$ University of Science and Gifted Education, had developed the multidisciplinary approach program of verifying the center of gravity for gifted students, but this program was reconstructed and applied to ordinary students and the effectiveness was analyzed to lay the foundation and generalize this convergence education. Including experiments for verifying the center of gravity in an object with a hollow interior and the existence of a center of gravity outside an object, I proposed realizing the calculations by considering the weight of the lever, the Principle of the lever being a core factor when finding the center of gravity. We altered the existing 8 step program to a 4 step program for the told 65 students from elementary, Junior and High School students, letting them freely select the class lecture by themselves. The analysis attained from surveys, debates and interviews showed that by precise error analysis, students achieved a higher success experience, showing us the importance of the development of a new convergence program.

• Journal of Korean Tunnelling and Underground Space Association
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• v.5 no.1
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• pp.71-88
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• 2003

The DRASTIC system is widely used for assessing regional groundwater pollution susceptibility by using hydrogeological factors such as depth to water, net recharge, aquifer media, soil media, topography, vadose zone media, hydraulic conductivity. This study is providing Modified Drastic Model to which lineament density, land use, influence of groundwater drawdown caused by tunnel excavation are added as additional factors using geographic information system, and then to evaluate groundwater contamination potential of ${\bigcirc}{\bigcirc}$ area. For statistical analysis, vector coverage per each factor is converted to grid layer and after each correlation coefficient between factors, covariance, variance, eigenvalue and eigenvector by principal component analysis of 3 direction, are calculated, correlation between factors is analyzed. Also after correlation coefficients between general DRASTIC layer and rated lineament density layer, between general DRASTIC layer and rated land use layer, between general DRASTIC layer and rated tunnel excavation influence layer are calculated, final modified DRASTIC model is constructed by using them with each weighting. When modified DRASTIC model was compared with general DRASTIC model, contamination potential in modified DRASTIC model is fairly detailed and consequently, vulnerable area which has high contamination potential could be presented concretly.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.847-853
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• 2014

There is a lot of debate on the relationship between vitamin D receptor polymorphisms and risk of breast cancer. Herein, we quantitatively analyzed the published case-control studies on this relationship by meta-analysis, performing a bibliographic search from Pubmed and CNKI up to July 31, 2013. The included case-control studies for Fok1, Bsm1, Taq1, Apa1, Cdx2 and Poly-A were 16, 19, 20, 10, 4, 6, respectively. Crude and adjusted odd ratios and 95% confidence intervals were calculated to present and compare the strength of any associations. The results of combined analyses indicated that Fok1, Bsm1, Apa1, Cdx2 and Poly-A were not significantly associated with the risk of breast cancer. In contrast, the tt genotype of Taq1 was a modest risk factor for breast cancer development (tt vs. TT: OR = 1.21, 95% CI: 1.01-1.44). To further confirm the above results, adjusted effects for the six polymorphisms were pooled based on adjusted ORs reported in the original studies. Adjusted ORs of Fok1, Apa1, Cdx2 and Poly-A were similar to the crude ORs. However, Bsm1 and Taq1 showed inconsistent results. For Bsm1, OR for BB vs. bb was 0.85, 95% CI: 0.74-0.98; for Taq1, OR for tt vs. TT was 1.03, 95% CI: 0.92-1.15, and not associated with risk. Subgroup analyses for crude ORs showed some association between Bsm1, Taq1 and breast cancer in Caucasians only, but for adjusted ORs, no associations were found. This meta-analysis suggests that the roles that Fok1, Apa1, Cdx2 and Poly-A polymorphisms play in breast cancer risk are negligible, with Bsm1 and Taq1 as possible exceptions. To be conservative, we still assumed that they may play a modest role in determining breast cancer risk. Further studies are needed to validate our findings.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.2
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• pp.93-108
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• 1990

Complete larval development Orithyia sinica (Linnaeus) is composed of three zoeal ins-tars and megalopa. Survival of instals was higher in $20^{\circ}C$ than in ambient temperature. Intermolt period generally increased with the increase of number of instals, but a notable decrease was observed from Crab-5 to prepuerty molt instals(Crab-7). Zoeas and me-galopa reared at higher temperature regime were samller than those reared at lower temperature. No temperature effect was shown in the size of crab instals. Growth factors fluctuate with the increase of number of instals, but there was an overall trend of net decrease. Growth factor was $35.65\~41.4\%$ for zoeal instals, and they were $18.00\~30.91\%$ for crab instals.

• Journal of Korean Society for Geospatial Information Science
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• v.6 no.2 s.12
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• pp.105-117
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• 1998

The main object of this study was to prove the effectiveness of satellite Image data for extraction of the pine needle gall midge damaged area in the part of Kangwon-do area, and to present the detailed procedure of a digital image processing for extraction of those damaged area. The effectiveness of extraction of damaged area was improved by using the BRCT(Backwards Radiance Correction Transformation) with DEM for the normalization of topographic effects. The topographic surface analysis of the extracted damaged area revealed that the general damaged area was at south-west and south-east aspect with the slope of 31 to 38 degrees, the temperature of 21 to 25, and 23% to 39% of the highest altitude mountains. The new damaged area in which expanded area was at 27 to 30 degree of slope, the aspect of 46 to 180 degrees, the temperature of $11^{\circ}C\;to\;12^{\circ}C$ and 27% to 39% of the highest altitude mountains. The NDI(New Damaged Index) was developed using the environment factor and simple vegetation index.