• 한국학교수학회논문집
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• 제8권4호
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• pp.481-494
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• 2005

본 연구는 대학에서 연구와 교육을 통하여 축적된 수학교육에 대한 노하우를 상대적으로 소외된 도서지역 고등학생들에게 제공함으로써 고급지식 및 이론의 확산에 힘쓰고자 한다. 연구는 삶의 지혜를 얻기 위한 수학, 실용능력을 배양하기 위한 실용수학, 자신의 품격을 높이기 위한 교양 수학으로 나누어 진행하며 수학 공부를 해야 하는 이유, 수학문제 해결력을 증진시키는 방안, 수학 기기를 이용한 수학학습, 암호이론 및 게임이론 소개, GSP를 이용한 도형학습, 수학과 스포츠, 수학과 예술 등을 포함하여 교재를 개발하고 개발된 교재를 통하여 대학원생을 훈련하고 수업계획안에 따라 수업을 하게 하는 도서지역 고등학생을 위한 흥미롭고 효과적인 수학지도 방안을 연구한다.

• 통상정보연구
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• 제9권2호
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• pp.19-40
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• 2007

In recent, T-commerce is widely dispersed as alternative type of commerce. It is forecasted that t-commerce system is used more than e-commerce system. Therefore more and more t-commerce-related industries are also recognizing that t-commerce is a critical business model. It is needed to understand the concept of t-commerce and develop the t-commerce marketing strategy. CEO analyses consumer's behaviors according to the data about buyers and applies the advantage of t-commerce to the communication with customers. This t-commerce system plays an important role in maximizing customer satisfaction and affecting their intention to reuse it. Therefore this paper attempts to identify T-commerce critical success factors and divide between use-intention group and unuse-intention group by taking out a discriminant function by the discriminant analysis. This lays a foundation in developing T-commerce strategy. According to the discriminant function extracted, convenience factor, amusement factor, system quality factor, product perception factor are significant in the sequence of influential degree. However, usefulness factor and speedy connection factor are not significant. In result, the target hitting rate is 77.9% in the first unuse-intention group and it is 95.2% in the second use-intention group. The total discriminant target hitting rate is computed to higher value, 86.55%. The statistic package, SPSS 12.0, is used to survey and analyse data and test the hypothesis. The validity and reliability of variables are verified by both reliability analysis and factor analysis. The discriminant analysis is used to tell the difference between use-intention group and unuse-intention group.

• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.1983-1993
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• 2017

Salmonella enterica subsp. enterica serovar Typhimurium, one of the most common foodborne pathogens, is transmitted mainly through contaminated food derived from infected animals. In this study, S. Typhimurium ST1120, an isolate from pig feces in Korea, was subjected to whole-genome analysis to understand its genomic features associated with virulence. The genome of ST1120 was found to have a circular chromosome of 4,855,001 bp (GC content 52.2%) and a plasmid of 6,863 bp (GC content 46.0%). This chromosome was predicted to have 4,558 open reading frames (ORFs), 17 pseudogenes, 22 rRNA genes, and 86 tRNA genes. Its plasmid was predicted to have three ORFs. Comparative genome analysis revealed that ST1120 was phylogenetically close to S. Typhimurium U288, a critical isolate in piggery farms and food chains in Europe. In silico functional analysis predicted that the ST1120 genome harbored multiple genes associated with virulence and stress resistance, including Salmonella pathogenicity islands (SPIs containing SPI-1 to SPI-5, SPI-13, and SPI-14), C63PI locus, ST104 prophage locus, and various antibiotic resistance genes. In accordance with these analysis results, ST1120 showed competence in invasion and survival abilities when it was added to host cells. It also exhibited robust resistance against antibiotics in comparison with other S. Typhimurium strains. This is the first report of the complete genome sequence of S. Typhimurium isolated from swine in Korea. Comparative genome analysis between ST1120 and other Salmonella strains would provide fruitful information toward understanding Salmonella host specificity and developing control measures against S. Typhimurium infection.

• 한국정밀공학회지
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• 제19권1호
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• pp.79-92
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• 2002

This paper describes a research work of developing computer-aided design of a product with bending and piercing for progressive working. An approach to the system for progressive working is based on the knowledge-based rules. Knowledge for the system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in AutoLISP on the AutoCAD with a personal computer and is composed of four main modules, which are input and shape treatment, flat pattern layout, strip layout and die layout modules. The system is designed by considering several factors, such as bending sequences by fuzzy set theory, complexities of blank geometry, punch profiles, and the availability of a press equipment. Strip layout drawing generated in the strip layout module is presented in 3-D graphic farms, including bending sequences and piercing processes with punch profiles divided into for external area. The die layout module carries out die design for each process obtained from the results of the strip layout. Results obtained using the modules enable the manufacturer for progressive working of electric products to be more efficient in this field.

• Molecules and Cells
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• 제28권2호
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• pp.131-137
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• 2009

Plant defensins are small (5-10 kDa) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. To understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the Brassica rapa Defensin 1 (BrD1) gene and introduced it into rice (Oryza sativa L.) to produce stable transgenic plants. The BrD1 protein is homologous to other plant defensins and contains both an N-terminal endoplasmic reticulum signal sequence and a defensin domain, which are highly conserved in all plant defensins. Based on a phylogenetic analysis of the defensin domain of various plant defensins, we established that BrD1 belongs to a distinct subgroup of plant defensins. Relative to the wild type, transgenic rices expressing BrD1 exhibit strong resistance to brown planthopper nymphs and female adults. These results suggest that BrD1 exhibits insecticidal activity, and might be useful for developing cereal crop plants resistant to sap-sucking insects, such as the brown planthopper.

• Journal of Mechanical Science and Technology
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• 제19권5호
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• pp.1072-1079
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• 2005

Conceptual design is the first step in the overall process of product design. Its intrinsic uncertainty, imprecision, and lack of information lead to the fact that current conceptual design activities in engineering have not been computerized and very few CAD systems are available to support conceptual design. In most of the current intelligent design systems, approach of principle synthesis, such as morphology matrix, bond graphic, or design catalogues, is usually adopted to deal with the concept generation, in which optional concepts are generally combined and enumerated through function analysis. However, as a large number of concepts are generated, it is difficult to evaluate and optimize these design candidates using regular algorithm. It is necessary to develop a new approach or a tool to solve the concept generation. Generally speaking, concept generation is a problem of concept synthesis. In substance, this process of developing design candidate is a combinatorial optimization process, viz., the process of concept generation can be regarded as a solution for a state-place composed of multi-concepts. In this paper, genetic algorithm is utilized as a feasible tool to solve the problem of combinatorial optimization in concept generation, in which the encoding method of morphology matrix based on function analysis is applied, and a sequence of optimal concepts are generated through the search and iterative process which is controlled by genetic operators, including selection, crossover, mutation, and reproduction in GA. Several crucial problems on GA are discussed in this paper, such as the calculation of fitness value and the criteria for heredity termination, which have a heavy effect on selection of better concepts. The feasibility and intellectualization of the proposed approach are demonstrated with an engineering case. In this work concept generation is implemented using GA, which can facilitate not only generating several better concepts, but also selecting the best concept. Thus optimal concepts can be conveniently developed and design efficiency can be greatly improved.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.118-118
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• 2008

The phylogenetic diversity of microbial communities in calcareous mats from Spearfish Creek, a freshwater stream located in the Black Hills of South Dakota, was examined using PCR-based 16S rDNA sequence analysis. In this study, two types of calcareous mats were compared: mature mats formed on the natural substrate of rock surfaces and developing mats on an artificial substrate of glass slides. Among 63 unique isolates from a clone library of 16S rRNA genes from mature mat samples, there were 8 phyla of Bacteria represented. The predominant phylum was Proteobacteria (48%), with the $\beta$ subclass being the largest group. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes from slide samples collected at intervals for four months showed considerable diversity of the microbial community from the earliest stages of community development. Amplicons isolated from DGGE gels and sequenced indicated that community succession has occurred without increasing microbial diversity. However, light microscopic analysis revealed a significant increase in microbial cell density throughout the community development. Scanning electron microscopy of mat samples provides evidence that diatoms are also important members of calcareous mat communities.

• 한국치위생학회지
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• 제8권4호
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• pp.101-113
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• 2008

The purpose of this study was to examine the health segment of the 7th national physical education curriculum in an effort to grasp the state of oral health education in the school curriculum. And it's also meant to investigate the relationship between oral health education and the oral health awareness of some high schoolers to check into any possible problems with oral health education as part of health education. The subjects in this study were 240 students from two different girls' high schools in Seoul, on whom a survey was conducted to find out their oral health knowledge, behavior and awareness. And the health category of the current P.E. curriculum announced by the Ministry of Education was analyzed. The findings of the study were as follows: Oral health education provided by the high schools as part of health education in P.E. class wasn't linked to P.E. theories and practice. The weight of oral health education was extremely small in the health segment, and there was no sequence, continuity and integration among the content systems of oral health education for different grades. When some high schoolers were investigated, they weren't properly oral health conscious, though it was very important for students to receive oral health education to prevent and manage dental diseases. And they didn't consequently take care of their own oral health. Future research efforts should be channeled from diverse angles into developing oral health education curriculums to bolster school oral health education to improve national oral health.

• 생명과학회지
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• 제21권3호
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• pp.468-472
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• 2011

DNA 마커는 유전현상 분석이나 품종육성에 널리 사용되고 있다. 본 연구에서는 내냉성 오이 계통인 'NC76'과 냉해 감수성 계통인 'GY14'로부터 내냉성 연관 마커을 목적으로 기존의 총 995개 SSR 마커의 다형성을 평가하였다. Agarose gel 전기영동법으로 'NC76과 'GY14' 간 PCR증폭 산물의 길이 다형성을 보이는 145개 SSR 마커를 개발하였으며, high resolution melting (HRM) 기술을 사용하여 염기서열 다형성을 보이는 30개의 SSR 마커를 확인하였다. 개발된 175개 SSR 마커 중 20개 마커를 선발하여 'NC76'과 'GY14' 간 $F_2$ 분리 집단에 대한 연관지도를 작성하였으며 그 결과 13개의 마커가 예상했던 연관군에 일치하여 위치됨을 확인할 수 있었다. 따라서 본 연구에서 확인된 175개의 SSR 마커는 향 후 냉해 저항성 연관 마커 개발을 위한 오이 유전자 지도 작성 및 이를 통한 품종 육성에 크게 활용될 수 있을 것으로 기대된다.

• 한국신뢰성학회:학술대회논문집
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• 한국신뢰성학회 2000년도 춘계학술대회 발표논문집
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• pp.239-246
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• 2000

이 논문은 중대사고가 발생하였을때 운전원에 의하여 사고관리 방안을 수행하는 경우 그 실현성(Feasibility)을, 평가하는데 사용할 수 있는 새로운 시간의존적 신뢰도 분석방법을 제시하였다. 이 방법은 성능요구 (Performance Requirement)와 성능성취 (Performance Achievement)의 상관관계의 개념을 이용하는 신뢰도물리(Reliability Physics)와 모든 시간의존적 사고경위를 도출하는 동적사건수목 생성방법에 기초하고있다. 신뢰도물리는 성능요구변수와 성능성취변수의 비교를 이용한 신뢰도분석방법인 반면 동적사건수목 생성방법은 바람직한 해를 얻을 때까지 모든 가능한 사고경위를 도출해 내는 방법이다. 이 방법론을 정전사고시 참조원전의 공동에 비상화재시스템을 이용하여 물을 공급하는 공동범람사고관리 방안에 적용시켰다. Latin Hypercube Sampling 방법은 성능요구변수의 불확실성을 평가하는데 사용되었다. 제시된 방법론은 사고시 필요한 운전원의 방안수행 성공가능성을 평가하는데 사용될 수 있을 뿐만 아니라 궁극적으로 사고관리 절차서 개발에 도움이 될 수 있음을 보여주었다.