• Journal of Society of Preventive Korean Medicine
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• v.11 no.1
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• pp.131-138
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• 2007

Herbal medicine has been traditional used for prevention and treatment of treatment of disease, because herbal medicine has few side effects in the human body. Anti-medicines such as antimicrobial medicine and anti-cancer medicine, which contains herbal medicine, are being studied vigorously by researchers. The effects of Geum Japonicum Thumb are restorative, blood circulation and detoxification. It also treats hematemesis, menstruation and furunculus. The ingredients of Geum Japonicum Thumb include geoside and tannin. The study measured antioxidative activation and antifungal activation by using Geum Japonicum Thumb water and methanol extract. The crude extract, which was used for this study, was Korean herbal medicine and the results are as follows. As Geum Japonicum Thumb extracts get stronger, the antioxidative activation tended to increase. The antioxidative activation hit its peak when the density of methanol extract was 120ug/ml. The experiment showed that a 1.0mm clear zone appeared when the density of the Geum Japonicum Thumb methanol extracts were 1000ug/ml in the Staphylococcus epidermidis and 1.1mm clear zone at Pseudomonas aeruginosa. The results of this study concludes that the antioxidative activation is lubricated in the Geum Japonicum Thumb extracts. In addition, the antifungal activation occurred in the fungus of the Staphylococcus epidermidis and the Pseudomonas aeruginosa.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.1
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• pp.75-80
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• 2016

This study was conducted to evaluate effect of chemical amendments on reducing bioavailable fraction of heavy metals in soil along with ecotoxicological effect on earthworms, Eisenia fetida. Three different chemical amendments, lime (L), steel slag (SS), and acid mine drainage sludge (AMDS), were applied with varied application ratio (1, 3, 5%). Heavy metal contaminated soil was mixed with chemical amedments and earthworms, Eisenia fetida, were cultivated for 28 days. Bioavailable fraction of heavy metals (Cd, Cu, Pb, and Zn) extracted with 0.1N HCl was monitored and also, mortality, growth, and metal concentration in earthworm were assessed. Result showed that all three amendments had high efficiency to reduce bioavailable fraction of heavy metals in soil. In particular, lime showed the highest reduction rate of Cu (63.9-87.7%), Pb (7.90-24.65%), and Zn (40.83-77.60%) among three amendments. No mortality of earthworm was observed during experimental period except 3% and 5% AMDS treatment indicating that application of chemical amendments is safe in terms of ecotoxicological aspect. However, no positive correlation was observed between reduction of bioavaialble fraction of heavy metals in soil and earthworms. Overall, application of chemical amendments in agricultural field can be adapted for reducing bioavailable fraction of heavy metals and detoxification in soil.

• Journal of The Korean Society of Clinical Toxicology
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• v.1 no.1
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• pp.6-11
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• 2003

Various forms of dialytic techniques are available for detoxification. Hemodialysis, hemoperfusion and hemofiltration (hemodialfiltration) are the main treatment modalities. Because these modalities are rather invasive and expensive, it must be decided in balance of the risk and benefit to the patient. The prime consideration in the decision is based on the clinical features of poisoning; hemodialysis or hemoperfusion should be considered in general if the patient's condition progressively deteriorates despite intensive supportive therapy. The hemodialysis technique relies on passage of the toxic agent through a semipermeable membrane so that it can equilibrate with the dialysate and subsequently removed. It needs a blood pump to pass blood next to a dialysis membrane, which allows agents permeable to the membrane to pass through and reach equilibrium. Solute (or drug) removal by dialysis has numerous determinants such as solute size, its lipid solubility, the degree to which it is protein bound, its volume of distribution etc. The technique of hemoperfusion is similar to hemodialysis except there is no dialysis membrane or dialysate involved in the procedure. The patient's blood is pumped through a perfusion cartridge, where it is in direct contact with adsorptive material (usually activated charcoal) that has a coating material such as cellulose. This method can be used successfully with lipid-soluble compounds and with higher-molecular-weight compounds than for hemodialysis. Protein binding does not significantly interfere with removal by hemoperfusion. In conclusion, hemodialysis, hemoperfusion and hemofiltration can be used effectively as adjuncts to the management of severely intoxicated patients.

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2001.12a
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• pp.126-126
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• 2001

The Rhus verniciflua contains alkly(en)-catechol type allergens with a saiurated or unsaturated alkly chain of 15 or 17 carbon atoms. It has been recognized as an extremely active allergen causing skin reactions similar In poison ivy. The allergic contact dermatitis induced by the urushiol is known to be mediated be T lymphocytes whicht specifically recognize the hepten urushiol. Therefore. direct use of this plant as a medicinal purpose might imply a considerable hazard in Korea. In this study, using the established method for the detoxification from the stem bark of Rhus verniciflua, an strong antioxidant substance was isolated and characterized DPPH (diphenypricryl hydrazyl) assay measures hydrogen atom-donating activity and hence provides a measure of free radical scavenging antioxidant activity. DPPH, a purple-colored stable free radical, is reduced to yellow-colored diphenylpicryl hydrazine by antioxidants to deducing agents. Antioxidative effects of the water extract from RV were measured by DPPH assay. Twenty microliters of the extract was added to 1ml of 100mM DPPH solution in ethanol The mixture was shaken and left to stand for 10min at room temperature. The crude water extracts was purified by using HPLC method with a DEAE (anionic type), CN, ODS column. The purified compound remained stable at pH 3.0-6,0, but unstable above pH 6.5. It was stable heat at 10$0^{\circ}C$ for 4 hours, but still had about 80% of residual activity after treatment at 10$0^{\circ}C$ for 5 hours. The elemental composition of the HR-EI mass spectrum at m/z 170.02 was estimated the empirical formula as $C_{7}$ $H_{6}$ $O_{5}$. $C_{10}$ $H_4$ $O_2$N$_1$, $C_{5}$ $H_4$ $O_4$N$_3$, $C_{8}$$H_2O$$_1$N$_4$. In antimicrobial test, no inhibition was observed against Gram-positive and negative bacteria. This compound was stronger than that of commercial antioxidant by DPPH test, such as BHT, BHC at the same concentration (20$\mu$g/ml).ml).

• The Journal of Internal Korean Medicine
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• v.22 no.1
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• pp.79-85
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• 2001

Object : Hepatitis Treatment-tang No.1 has been used for the treatment of Liver disease and Jaundice. Long-term EtOH exposure leads to immunoregulatory and detoxification impairment. This study aimed to determine the relationship between TNF-${\alpha}$ production and expression, and EtOH-induced cytotoxicity on Hep G2 cells. Method : Cells were incubated with EtOH in the presence or absence of HT. The cells were tested after 24 hours and, again, after 48 hours. Cytoviability and TNF-${\alpha}$ release were analyzed by MTT assay and enzyme linked immunosorbent assay (ELISA), respectively. After 24 hours of EtOH exposure, the cytoviability decreased, and the release of TNF-${\alpha}$ was increased. Increased amounts of TNF-${\alpha}$ contribute to EtOH-induced cytotoxicity. The Anti-TNF-${\alpha}$ antibody almost abolished it. Interestingly, EtOH-induced cytotoxicity and TNF-${\alpha}$ production were inhibited by HT. Moreover, when HT was used in combination with the anti-TNF-${\alpha}$ antibody, there was a marked inhibition of EtOH-induced cytotoxicity. Results : These results suggest that HT may prevent the cytotoxicity through partial inhibition of the TNF-${\alpha}$ secretion.

• Journal of Korean Medical classics
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• v.29 no.1
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• pp.113-123
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• 2016

Objectives : In this study, we tried to analyze the relationship between effect terms and five tastes in heat-clearing herbs. In order to analyze exactly, we also attempted to extract and refine the effect terms. And finally, we tried to infer the effects of each tastes and suppose new effects of each heat-clearing herbs based on their tastes. Methods : Firstly, we extracted the five tastes and effect terms among the heat-claearing herbs written in Herbology. After that, we divided each effect terms to single meaning term and refined them. We also grouped herbs by tastes and set the effect terms in the order of frequency. So we could analyze the relationship between the tastes and the effect terms and supposed the effect of each herbs. Results & Conclusions : We extracted 7 tastes and 87 effect terms. And they was refined to 63 effect terms. As the result of analyzing the effect terms based the tastes, all of heat-clearing herbs included the effects of heat-clearing and detoxification, regardless of tastes. The herbs which have the bitter tastes, included the effects of drying dampness, fire-purging and blood-cooling. And we supposed new effects in 10 kinds of herbs among them. The herbs which have the sweet tastes, included the effects of blood-cooling and fluid-engendering. The herbs which have the hot tastes, included the effects of inducing diuresis and swelling-dispersing. And we supposed new effects in 2 kinds of herbs, by analyzing the relationship between the tastes and effect terms.

• Resources Recycling
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• v.21 no.4
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• pp.76-85
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• 2012

Fluorescent lamps generally involve mercury within their lamp bulbs. Thus if the lamps are disposed as they are, it might cause environmental pollution. On the other hand, as the life of the lamps depends on the degradation of the discharge performance, the fluorescent substance composing the fluorescent substance coating can be recycled by reusing them as the recycled fluorescent substance. Also, the glass material composing the glass bulbs can be reused. The range of search was limited in the open patents of USA (US), European Union (EU), Japan (JP), Korea (KR) and SCI journals from 1977 to 2011. Patents and journals were collected using key-words searching and filtered by filtering criteria. The trends of the patents and journals was analyzed by the years, countries, companies, and technologies. Recovery method of metals from spent fluorescent lamps is mainly the focus on the recovery of precious metals using acid treatment and, detoxification technology is related with controlling process of mercury using a filter system.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.4
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• pp.684-692
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• 2002

Cordyceps militaris has been known as a Chinese traditional medicine for the treatment of tuberculosis, asthma, kidney disease, debility and fatigue etc. This study was attempted to investigate the therapeutic effect of C. militaris extract on the cytotoxic activity of HepG2, human hepatocellular carcinoma cells and the liver damage induced by carbon tetrachloride in SD rats. C. militaris extracts inhibited significantly the proliferation of HepG2 cells in vitro. Carbon tetrachloride(CCl₄) caused a significant an increase in liver weight, serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activity, alkaline phosphatase(ALP), serum thiobarbituric acid reactive substances (TBARS), microsomal TBARS, and decrease in microsomal detoxification enzymes (cytochrome P-450, P-450 reductase, cytochrome b5, b5 reductase). TBARS and ALP in serum pretreated with C. militaris extracts (300mg/kg/day, 600mg/kg/day) was significantly reduced compared to control group(CCl₄). Cytochrome b5 and b5 reductase activities were significantly increased in CM300 (300 mg/kg/day) and CM600 group(600 mg/kg/day), and cytochrome P-450 reductase was significantly increased in CM300 group. Pretreatment (100, 300, and 600 mg/kg/day for 7 days) of C. militaris with CCl₄ was significantly inhibited the accumulation microsomal TBARS and the significantly increased in the cytochrome P-450 activity. These results suggested that C. militaris (300mg/kg/day for 7 days) has appreciable therapeutic effect on CCl₄ induced hepatotoxicity.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.928-935
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• 2012

World Agriculture faces daunting challenges in feeding the growing population today. Reduction in arable land extent due to numerous reasons threatens achievement of food and nutritional security. Under this back ground, agricultural use of acidic soils, which account for approximately 40 % of the world arable lands is of utmost important. However, due to aluminum (Al) toxicity and low available phosphorous (P) content, crop production in acidic soils is restricted. Citrus, in this context, gains worldwide recognition as a crop adapted to harsh environments. The present paper reviewed Al toxicity and possible toxicity alleviation tactics in citrus. As reported for many other crops, inhibition of root elongation, photosynthesis and growth is experienced in citrus also due to Al toxicity. Focusing at toxicity alleviation, interaction between boron (B) and Al as well as phosphorus and Al has been discussed intensively. Al toxicity in citrus could be alleviated by P through increasing immobilization of Al in roots and P level in shoots rather than through increasing organic acid secretion, which has been widely reported in other crops. Boron-induced changes in Al speciation and/or sub-cellular compartmentation has also been suggested in amelioration of root inhibition in citrus. Despite the species-dependent manner of response to Al toxicity, many commercially important citrus species can be grown successfully in acidic soils, provided toxicity alleviation Agro-biological tactics such as addition of phosphorous fertilizers are used properly.

• Economic and Environmental Geology
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• v.34 no.4
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• pp.345-354
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• 2001

The effects on arsenic geochemistry of indigenous microorganisms isolated from an area contaminated with high concentration of arsenic were investigated. Arsenite exerted higher inhibitory effects on the microbes' growth than arsenate. During incubation of the microbes in an arsenate-spiked medium over 24 hours, decrease in microbial growth was observed as arsenate content increased. Arsenate of 150 mM or over apparently inhibited cell growth. However, further incubation for up to 4 days in the high arsenate concentration medium resulted in cell growth, implying that the microorganisms adjusted their biochemical functions to detoxify arsenic and maintain growth. Two types of microbes were observed during 20 hours to reduce arsenate to arsenite in solution through a detoxification mechanism. As well, decrease in the total arsenic content occurred over a 4-day incubation with the same microbes in an arsenate-spiked medium. Therefore it is suggested that microorganisms can influence arsenic speciation in natural settings and this may be applied to efficient bioremediation of arsenic-contaminated sites.