• 한국식물병리학회지
• /
• 제14권2호
• /
• pp.177-183
• /
• 1998

A DNA fragment which is involved in tolassin production was cloned to obtain a molecular marker of Pseudomonas tolaasii, a casual agent of bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). Tolaasin is a lipodepsipeptide toxin and known as a primary disease determinant of the P. tolaasii. It is responsible for formation of white line in agar when P. tolaasii were cultured against white line reacting organisms (WLROs). White line negative mutants (WL-) were generated by conjugation between rifampicin resistant strain of P. tolaasii and E. coli carrying suicidal plasmid pSUP2021 : : Tn5. The ability of tolaasin production of the WL- mutants was examined by hemolysis test, pathogenicity test, and high pressure liquid chromatography (HPLC) analysis of culture filtrate. All of the WL- mutants were lost the ability of tolaasin production (Tol-). Genomic library of the Tol- mutant was constructed in pLAFR3 and the cosmid clone containing Tn5 was selected. DNA fragment fro franking region of Tn5 was cloned from the plasmid and used as a probe in Southern blot. DNA-DNA hybridization with the probe to total DNA from group of bacteria ecologically similar to P. tolaasii including WLORs, fluorescent Pseudomonads isolated from oyster mushroom, P. agarici, P. gingeri, and some of other species of Psedomonas showed that some of the tested bacteria do not have any hybridized band and others have bands sowing RFLP. The cloned DNA fragment or its nucleotide sequence will be useful in detection and identification of the P. tolaasii.

• 대한기계학회논문집A
• /
• 제28권11호
• /
• pp.1711-1718
• /
• 2004

When the tracked vehicle is running on various types of terrain, the physical properties of the interacting ground can be different. In this paper, the interactions between track link and soft soil ground are investigated using static sinkage theory of soil ground. Grouser surfaces of a track link and triangular patches of ground are implemented for contact detection algorithm. Contact force at each segment area of a track link is computed respectively by using virtual work concept. Bekker's static soil sinkage model is applied for pressure-sinkage relationship and shear stress-shear displacement relationship proposed by Janosi and Hanamoto is used for tangential shear forces. The repetitive normal loads of a terrain are considered because a terrain element is subject to the repetitive loading of the roadwheels of a tracked vehicle. The methods how to apply Bekker's soil theory for multibody track system are proposed in this investigation and demonstrated numerically by high mobility tracked vehicle.

• 에너지공학
• /
• 제13권3호
• /
• pp.205-213
• /
• 2004

본 연구에서는 고가인 고속도 CCD카메라가 아닌 비교적 간단한 광파이버 시스템을 이용한 자려진동연소 발생에 관한 검출과, 진동연소가 발생하기 전에 이를 미리 예견할 수 있는 통계학적 수법에 관해 조사를 하였다. 먼저 자려진동연소의 발생시에 사용될 수 있는 광파이버의 유용성이 검증되었다. 광파이버에 의해 검출되어진 OH 라디칼 자발광 강도는 고속도 CCD카메라에 의해 얻어진 값과 강한 자려진동연소가 발생할 때에 거의 일치를 하였다. 자려진동연소가 발생하면 시스템과 연소기기에 손상을 가하게 된다. 이러한 피해가 발생하기 전에 자려진동연소의 발생 징후를 미리 포착하려는 시도를 하였다. 이를 위해 압력과 OH라디칼 자발광과 같은 화염에서의 변동성분께 대한 통계학적 처리를 이용하여 자려진동연소의 발생을 예견할 수 있는 독특한 수법에 관해 조사를 하였다.

• 대한의용생체공학회:의공학회지
• /
• 제27권5호
• /
• pp.245-249
• /
• 2006

The aim of this study is to design and develop the gait rehabilitation equipment that judge patient's movement of his/her center of gravity using pressure sensors, and to aid hemiplegic patients to balance themselves using an automatic stepper that changes the patient's center of gravity. It is hard to bear the weight on the affected side for hemiplegic patients. The gait rehabilitation equipment detects the footing phase of hemiplegic patient during training and moves the unaffected footing side of the stepper up and moves the affected footing side down simultaneously so that the patient's center of gravity can shift from unaffected side to affected side. The gait rehabilitation system was developed and applied for hemiplegic patients during exercise. Eight hemiplegic patients and one normal adult were studied. The developed gait rehabilitation system could judge not only the normal adult's intention but also the patient's intention to move his/her center of gravity. Even though the most of hemiplegic patients exercised in automatic mode and a few hemiplegic patients exercised in manual mode, the developed gait rehabilitation system can aid the hemiplegic patients to train more easily.

• Nuclear Engineering and Technology
• /
• 제53권6호
• /
• pp.1846-1857
• /
• 2021

Ultrasonic nondestructive testing is important for monitoring the structural integrity of dissimilar metal welds (DMWs) in pressure vessels and piping in nuclear power plants. However, there is a low probability of crack detection via inspection of DMWs using ultrasonic waves because the grain structures (grain orientations) of the weld area cause distortion and splitting of ultrasonic beams propagating in anisotropic media. To overcome this issue, the grain orientation should be known, and a precise ultrasonic wave simulation technique in anisotropic media is required to model the distortion and splitting of the waves accurately. In this study, a method for nondestructive prediction of the DMW grain orientations is presented for accurate simulation of ultrasonic wave propagation behavior in the weld area. The ultrasonic wave propagation behavior in anisotropic media is simulated via finite-element analysis when ultrasonic waves propagate in a transversely isotropic material. In addition, a methodology to predict the DMW grain orientation is proposed that employs a simulation technique for ultrasonic wave propagation behavior calculation and an optimization technique. The simulated ultrasonic wave behaviors with the grain orientations predicted via the proposed method demonstrate its usefulness. Moreover, the method can be used to determine the focal law in DMWs.

• 한국기계가공학회지
• /
• 제20권4호
• /
• pp.106-112
• /
• 2021

Thermoplastic microfluidic devices are used in BioMEMS for medical and biotechnology applications, such as gene extraction, DNA analysis, and virus detection. In this research, a simple fabrication protocol with a commercially available pneumatic hot press is proposed and demonstrated for polycarbonate microfluidic devices. Microfluidic channels with a width of 200 ㎛ and a height of 10 ㎛ were designed and machined onto a brass plate as a mold insert using a CNC milling machine. The resulting microfluidic channels on the mold insert were assessed and found to have an actual width of 198 ㎛ and a height of 10 ± 0.25 ㎛. The microfluidic channels were replicated on a polycarbonate sheet using the proposed replication technique at 146℃ for 20 minutes under a constant load of 2400 kgf. The devices were then naturally cooled to 100℃ while maintaining the same pressure. It was found that the microchannels were successfully replicated in the polycarbonate, with a width of 198 ㎛ and a height of 10.07 ㎛. The proposed replication technique thus offers the rapid mass production of high-quality microfluidic devices at a low cost with a process that, unlike conventional photolithography systems, does not require expensive equipment.

• 한국기계가공학회지
• /
• 제19권12호
• /
• pp.80-86
• /
• 2020

With the exception of welding activities, it is forbidden to use electricity in shipyards, owing to safety concerns such as the possibility of fire, explosions, and short circuits. In this paper, an automatic chamfering machine using pneumatics is proposed for use in such environments. Customers specify their requirements and the machine derives the corresponding theoretical design conditions. The proposed machine was used to perform 3D modeling, and its suitability and performance were confirmed via cutting experiments of the manufactured device. Two types of sensors may be used in this system: contact and non-contact. In the case of the contact type, an end-stop switch that can recognize the end of the material is installed, and when the machine reaches the end of the material, the end-stop switch is operated to cut off the air pressure. In the non-contact type, four sensors were used: photonic, ultrasonic, metal detection, and encoder. The use of the four sensors was repeated 30 times, and the average error determined. Thus, the optimum sensor was identified.

• 한국안전학회지
• /
• 제36권4호
• /
• pp.1-11
• /
• 2021

Piping systems comprising pumps and valves are essential in the power plant, oil, and defense industry. Their purpose includes a stable supply of the working fluid or ensuring the target system's safe operation. However, piping system accidents due to leakage of toxic substances, explosions, and natural disasters are prevalent In addition, with the limited maintenance personnel, it becomes difficult to detect, isolate, and reconfigure the damage of the piping system and recover the unaffected area. An autonomous recovery piping system can play a vital role under such circumstances. The autonomous recovery algorithms for the piping system can be divided into low-pressure control algorithms, hydraulic resistance control algorithms, and flow inventory control algorithms. All three methods include autonomous opening/closing logic to isolate damaged areas and recovery the unaffected area of piping systems. However, because each algorithm has its strength and weakness, appropriate application considering the overall design, vital components, and operating conditions is crucial. In this regard, preliminary research on algorithm's working principle, its design procedures, and expected damage scenarios should be accomplished. This study examines the characteristics of algorithms, the design procedure, and working logic. Advantages and disadvantages are also analyzed through simulation results for a simplified piping system.

• 한국축산식품학회지
• /
• 제41권5호
• /
• pp.883-893
• /
• 2021

Clostridium difficile present in feces of food animals may contaminate their meats and act as a potential source of C. difficile infection (CDI) to humans. C. difficile resistance to antibiotics, its production of toxins and spores play major roles in the pathogenesis of CDI. This is the first study to evaluate C. difficile prevalence in retail raw animal meats, its antibiotics susceptibilities and toxigenic activities in Al-Jouf, Saudi Arabia. Totally, 240 meat samples were tested. C. difficile was identified by standard microbiological and biochemical methods. Vitek-2 compact system confirmed C. difficile isolates were 15/240 (6.3%). Toxins A/B were not detected by Xpect C. difficile toxin A/B tests. Although all isolates were susceptible to vancomycin and metronidazole, variable degrees of reduced susceptibilities to moxifloxacin, clindamycin or tetracycline antibiotics were detected by Epsilon tests. C. difficile strains with reduced susceptibility to antibiotics should be investigated. Variability between the worldwide reported C. difficile contamination levels could be due to absence of a gold standard procedure for its isolation. Establishment of a unified testing algorithm for C. difficile detection in food products is definitely essential to evaluate the inter-regional variation in its prevalence on national and international levels. Proper use of antimicrobials during animal husbandry is crucial to control the selective drug pressure on C. difficile strains associated with food animals. Investigating the protective or pathogenic potential of non-toxigenic C. difficile strains and the possibility of gene transfer from certain toxigenic/ antibiotics-resistant to non-toxigenic/antibiotics-sensitive strains, respectively, should be worthy of attention.

• Current Optics and Photonics
• /
• 제6권2호
• /
• pp.137-142
• /
• 2022

Leak detection is one of the most important challenges in condition monitoring of water pipelines. Fiber Bragg grating (FBG) sensors offer an attractive technique to detect leak signals. In this paper, leak measurements were conducted on a water distribution pilot plant with a length of 270 m and a diameter of 100 mm. FBG sensors were installed on the pipeline surface and used to detect leak vibration signals. The leak was demonstrated with 1-, 2-, 3-, and 4-mm diameter leak holes in four different pipe types. The frequency response of leak signals was analyzed by fast Fourier transform analysis in real time. In the experiment, the frequency range of leak signals was approximately 340-440 Hz. The frequency shifts of leak signals according to the pipe type and the size of the leak hole were demonstrated at a pressure of 1.8 bar and a flow rate of 25.51 m³/h. Results show that frequency shifts detected by FBG sensors can be used to detect leaks in pipelines.