• Journal of fish pathology
• /
• v.26 no.3
• /
• pp.185-191
• /
• 2013

The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was $2{\times}10^5$ PFU/ml with $1{\times}10^7$ CFU/ml of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage ($2{\times}10^5$ PFU/ml). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

• Proceedings of the KWS Conference
• /
• 2009.11a
• /
• pp.73-73
• /
• 2009

In this sutdy, a new class ACA(Anisotropic Conductive Adhesive) with low-melting-point alloy(LMPA) and self-organized interconnection method were developed. This developed self-organized interconnection method are achieved by the flow, melting, coalescence and wetting characteristics of the LMPA fillers in ACA. In order to observe self-interconnection characteristic, the QFP($14{\times}14{\times}2.7mm$ size and 1mm lead pitch) was used. Thermal characteristic of the ACA and temperature-dependant viscosity characteristics of the polymer were observed by differential scanning calorimetry(DSC) and torsional parallel rheometer, respectively. A electrical and mechanical characteristics of QFP bonding were measured using multimeter and pull tester, respectively. Wetting and coalescence characteristics of LMPA filler particles and morphology of conduction path were observed by microfocus X-ray inspection systems and cross-sectional optical microscope. As a result, the developed self-organized interconnection method has a good electrical characteristic($2.41m{\Omega}$) and bonding strength(17.19N) by metallurgical interconnection of molten solder particles in ACA.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.34 no.4
• /
• pp.12-29
• /
• 2021

Objective: This study was performed to investigate the inhibitory effect of Cheongpochukeo-tang (CCT) on lipopolysaccharide (LPS)-induced inflammation model. Methods: RAW 264.7 cells were pre-treated with CCT and incubated with LPS (500 ng/ml) after 1 hour. Cell viability was measured by MTT assay to figure out cytotoxicity of CCT. The production of nitric oxide and mRNA expression of pro-inflammatory cytokine were measured. And the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) were examined to figure out molecular mechanisms of CCT's anti-inflammatory effects. In addition, mice survival rate and cytokine levels of serum were observed after treated with CCT. And mice liver tissues were observed and their cytokines levels in liver tissue were measured. Results: CCT did not have cytotoxic effect in RAW 264.7 cells. It inhibited LPS-induced nitric oxide (NO) production, but showed an increase in NO by itself at 2 mg/ml concentration. CCT inhibited mRNA expression of IL-1β, IL-6, TNF-α in a dose dependant and the activaton of MAPKs and NF-κB. In addition, CCT reduced mortality in the LPS-induced mouse model and inhibited production of cytokines in mouse serum and liver tissue. Conclusion: The results suggest that CCT could reduce LPS-induced inflammation by inhibiting MAPKs and NF-κB activaton, NO production, and pro-inflammatory cytokines secretion. Thereby, CCT could be effective medicine for the inflammatory disease.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.34 no.2
• /
• pp.1-20
• /
• 2021

Objective : The object of this study was to assess the efficacy of hydrolysate from Phellinus igniarius(HPI) on anti-aging activities in vitro measurement and mini clinical study performed on 5 subjects. Methods : To evaluate skin anti-aging efficacy of HPI, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, type I collagen synthesis, inhibition of nitric oxide(NO) production, inhibiton of tyrosinase, hyaluronan synthase(HAS)2, 3 mRNA expression were measured in vitro. Also, mini clinical study of skin hydration was performed on 5 subjects using HPI in distilled water(DW) and 1,3-butylene glycol diluted solution(30% in DW). Results : 1. DPPH radical scavenging activity of HPI was increased in a dose-dependant. 2. Type I collagen synthesis was increased in 50, 100 and 500㎍/㎖ of HPI. 3. NO production was not inhibited in all concentrations of HPI. 4. Tyrosinase was inhibited in 500, 1000, 2500 and 5000㎍/㎖ of HPI. 5. HAS2 mRNA expression was increased in 50, 100, 150 and 200㎍/㎖ of HPI, HAS3 mRNA expression was increased in 100, 150, and 200㎍/㎖ of HPI. 6. In the mini clinical study of 5 subjects, there was a difference in skin hydration over time for each solutions, but it was not statistically siginificant. Conclusions : HPI increased DPPH radical scavenging activity, type I collagen synthesis, and HAS2, HAS3 mRNA expression. HPI also suppressed tyrosinase. The findings of this study suggest that HPI can be used as an skin anti-aging material.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2014.10a
• /
• pp.189-192
• /
• 2014

Recently, U.S. and Europe, which are advanced to Air Traffic Control Management, are interest in replacing Radar System with Communications, Navigation and Surveillance(CNS) and Air Traffic Management(ATM). They pursue to research the efficient Air Traffic Control Management. This paper covers ADS-B 1090ES system which is one of CNS/ATM surveillance systems. This research satisfied all performance required by RTCA DO-268B and EUROCAE ED-129. It optimized algorism to mainly enhance performance such as quality of receiver signal, dynamic range, and so on. The optimized mechanism provided stable performance of demodulation, tuned the level of signal, and had reduced the false reception ratio by the signal level difference. The analyzed algorism helped great performance and will be considered to apply broad system applications.

• Journal of the Korean Geosynthetics Society
• /
• v.18 no.3
• /
• pp.55-67
• /
• 2019

Deep soft ground in mouth of Nackdong river requires to be analysed with prediction method concerning characteristics of secondary consolidation from the beginning because it causes excessive settlement due to time-dependant secondary consolidation characteristics. This study investigated characteristics of extended settlement by conducting one-dimensional theory, elasto-plastic model and visco-elasto-plastic model as well as analyzing long-term measuring data observed over 2,000 days. According to one-dimensional theory and elasto-plastic model, there is not definite correlation between height of embankment and depth of soft ground while visco-elasto-plastic model showed similar result of settlement to that of long-term measuring data. Consequently it is suggested that applying visco-elasto-plastic model to developing deep underground place as studied area on predicting extended settlement before construction prevents economic loss and delay during process by preparing secondary consolidation characteristics.

• Journal of Pharmacopuncture
• /
• v.23 no.4
• /
• pp.212-219
• /
• 2020

Objectives: Nowadays cancer treatment is an important challenge in the medical world that needs better therapies. Many active secretions produced by insects such as honey bees used to discover new anticancer drugs. Bee venom (BV) has a potent anti inflammatory, anti cancer and tumor effects. The aim of present study is evaluation of anticancer effects induced by Apis mellifera venom (AmV) on cell Lines. Methods: AmV was selected for study on cancer cell lines. Total protein, molecular weight and LD50 of crude venom were determined. Then, cells were grown in Dulbecco's Modified Eagle medium supplemented with 10% fetal bovine serum and 1% antibiotics. The A549, HeLa and MDA-MB-231 cell Lines were exposed by different concentration of AmV. The morphology of cells was determined and cell viability was studed by MTT assay. Evaluation of cell death was determined by and DNA fragmentation. Results: The results from MTT assay showed that 3.125 ㎍/mL of A549, 12.5 for HeLa and 6.25 ㎍/mL of MDA-MB-231 killed 50% of cells (p < 0.05). Morphological analysis and the results from hoescht staining and DNA fragmentation indicated that cell death induced by AmV was significantly apoptosis. Conclusion: The data showed that using lower dosage of AmV during treatment period cause inhibition of proliferation in time and dose dependant manner. Findings indicated that some ingredients of AmV have anticancer effects and with further investigation it can be used in production of anticancer drugs.

• Journal of the Korea Convergence Society
• /
• v.12 no.2
• /
• pp.221-231
• /
• 2021

This paper identify factors of migration of employees' household who work for relocated public institutions. As a factors of migration, we consider individual and household characteristics, the gravity model of distance and population and so on. Considering discrete dependant variable and structure of data, we employ the logistic multilevel model and random intercept model. The result indicates employees' who are female, 30s and 40s, higher education level(PhD) and whose spouse are unemployed tend to transfer their residential registration to new city near relocated public institution. Regarding regional variable, the distance from employee's previous residential location and number of migration of prior year are statistically significant. Also the model indicate regional economy, educational and residential environment of new city influence employee's decision for transferring residential registration.

• English & American cultural studies
• /
• v.9 no.1
• /
• pp.51-70
• /
• 2009

For a long time Westerners have considered the Orient as unknown and mysterious, but Orientals soon came to be seen as weak and dependent, or feminine. The Oriental woman became a synecdoche for the Orient itself. We can find this theme in several British plays that deal with the Orient and Oriental women, including Shakespeare's Antony and Cleopatra and Dryden's All for Love. Both of these plays have Egypt as their setting and Cleopatra as a main character. For a better society, Shaw emphasizes the importance of education. In Caesar and Cleopatra, Shaw sees Egypt as a weak and dependent country which needs the help of Rome. Accordingly, he depicts Cleopatra as young and ignorant, needing to be educated in her role as a queen. Shaw finds possibilities for growth and independence in the Egyptians and Cleopatra, who recognize themselves as Egyptians and pursue their identity apart from the colonialization of Rome. Here the Egyptians attempt to resist and escape the oppression of Rome. Young, dependant and ignorant Cleopatra becomes independent and knowledgeable as the result of her education by Caesar and in the end she becomes a real Egyptian queen. According to Shaw, the Orient and women have the potential to develop themselves and ultimately to overcome the government of Western countries and men. In this play, Shaw emphasizes the potential of the Orient and women and the importance of education. Shaw thinks women can grow and develop through education. Especially through Cleopatra's growth, his thought can be applied for Oriental women as well as Western women. His thought is beyond the 19th century British society in which this play was written. Through this play, we can see Shaw's thought is not limited by race, time and place and also has universality to transcend everything.

• The Korean Journal of Food And Nutrition
• /
• v.34 no.6
• /
• pp.662-668
• /
• 2021

Aster koraiensis Nakai (A. koraiensis) which has been used as a food and medicinal plant in the past, is valuable as functional food material. Therefore, the aim of this study was to determine the antioxidant properties and major phenolics of A. koraiensis extracts with different ethanol concentrations (0, 50, 70, and 100% aqueous ethanol solution). When ethanol concentration in the extraction solvent was increased, extraction yield decreased; 34.2, 23.2, 21.0, and 5.5% in 0, 50, 70, and 100% ethanolic extracts, respectively. Total phenolics content and antioxidant activities of extracts were increased in an ethanol concentration-dependant manner. The major phenolics in the extracts were chlorogenic acid (21.264~58.666 mg/g), isochlorogenic acid A (10.432~145.353 mg/g), and isochlorogenic acid C(0.239~13.148 mg/g), and these phenolic contents were higher in 70 and 100% ethanolic extracts than other extracts. Significant correlations were observed between ethanol concentration of extraction solvent, antioxidant properties, and major phenolics. These results indicated that the optimal ethanol concentration for extraction was 70%.