• Journal of Ginseng Research
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• v.36 no.4
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• pp.449-460
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• 2012

Plants have versatile detoxification systems to encounter the phytotoxicity of the wide range of natural and synthetic compounds present in the environment. Glutathione S-transferase (GST) is an enzyme that detoxifies natural and exogenous toxic compounds by conjugation with glutathione (GSH). Recently, several roles of GST giving stress tolerance in plants have demonstrated, but little is known about the role of ginseng GSTs. Therefore, this work aimed to provide further information on the GST gene present in Panax ginseng genome as well as its expression and function. A GST cDNA (PgGST) was isolated from P. ginseng cDNA library, and it showed the amino acid sequence similarity with theta type of GSTs. PgGST in ginseng plant was induced by exposure to metals, plant hormone, heavy metals, and high light irradiance. To improve the resistance against environmental stresses, full-length cDNA of PgGST was introduced into Nicotiana tabacum. Overexpression of PgGST led to twofold increase in GST-specific activity compared to the non-transgenic plants, and the GST overexpressed plant showed resistance against herbicide phosphinothricin. The results suggested that the PgGST isolated from ginseng might have a role in the protection mechanism against toxic materials such as heavy metals and herbicides.

• Journal of the Korean Society for information Management
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• v.28 no.2
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• pp.135-158
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• 2011

The ability of the searcher to express their information problem to an information retrieval system is fundamental to the retrieval process. Query by visual example is the principal query paradigm for expressing queries in a content-based image retrieval environment yet there is little empirical evidence to support its efficacy in facilitating query formulation. The aim of this research was to investigate the usability of the query by colour method in supporting a range of information problems in order to contribute to the gap in knowledge regarding the relationship between searchers' information problems and the query methods required to support efficient and effective visual query formulation. The results strongly suggest that the query method does not support visual query formulation and that there is a significant mismatch between the searchers information problems and the expressive power of the retrieval paradigm.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.2
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• pp.139-143
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• 2021

In this study, data repository information registered in re3data (re3data.org), a research data registry, was collected. Based on collected data, the current status was analyzed for 354 repositories (approximately 14% of total repositories) in the field using keywords in the ecological field suggested by two experts. Major metadata formats used to describe data in ecological research data repositories include Federal Geographic Data Committee Content Standard for Digital Geospatial Metadata (FGDC/CSDGM), Dublin Core, ISO 19115, Ecological Metadata Language (EML), Directory Interchange Format (DIF), Darwin Core, Data Documentation Initiative (DDI), and DataCite Metadata Schema. The number of ecological repositories according to country is 102 in the US, 34 in Germany, 31 in Canada, and one in Korea. A total of 771 non-profit organizations and 12 for-profit organizations are involved in the construction of the ecological field research data repository. Data version control ratio of the ecological field research data repositories registered in re3data was analyzed to be somewhat higher (86.6%) than the total ratio (83.9%). Results of this study can be used to establish policies to build and operate a research data repository in the ecological field.

• Journal of the Korean Society for information Management
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• v.27 no.2
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• pp.241-252
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• 2010

Titles have been regarded as having effective clustering features, but they sometimes fail to represent the topic of a document and result in poorly generated document clusters. This study aims to improve the performance of document clustering with titles by suggesting titles in the citation bibliography as a clustering feature. Titles of original literature, titles in the citation bibliography, and an aggregation of both titles were adapted to measure the performance of clustering. Each feature was combined with three hierarchical clustering methods, within group average linkage, complete linkage, and Ward's method in the clustering experiment. The best practice case of this experiment was clustering document with features from both titles by within-groups average method.

• JSTS:Journal of Semiconductor Technology and Science
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• v.15 no.2
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• pp.255-266
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• 2015

In this paper, a low-complexity and low-power soft output multiple input multiple output (MIMO) symbol detector is proposed for mobile devices with two transmit and two receive antennas. The proposed symbol detector can support both the spatial multiplexing mode and spatial diversity mode in single hardware and shows the optimal maximum likelihood (ML) performance. By applying a multi-stage pipeline structure and using a complex multiplier based on the polar-coordinate, the complexity of the proposed architecture is dramatically decreased. Also, by applying a clock-gating scheme to the internal modules for MIMO modes, the power consumption is also reduced. The proposed symbol detector was designed using a hardware description language (HDL) and implemented using a 65nm CMOS standard cell library. With the proposed architecture, the proposed MIMO detector takes up an area of approximately $0.31mm^2$ with 183K equivalent gates and achieves a 150Mbps throughput. Also, the power estimation results show that the proposed MIMO detector can reduce the power consumption by a maximum of 85% for the various test cases.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.8
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• pp.1511-1516
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• 2016

Sports areas of the Republic of Korea has been strengthened by the sports powers in various phases of the competition. However, not all students can succeed in greatly athlete, was necessary for these measures. This paper presents the design and implementation of mobile applications to athletic coaching leaders to record dynamic data of student athletes. Stocks applicable in this paper is basketball. The development of mobile applications can choose to 'Assist Records' menu and 'Hot & Cold Zone' menu in the Main Page. Athletic director are stored in the photo file after the screen capture to allow the map to be just athletes. To a point that can be expected can be used as the training time and increasing efficiency of the training effect through the mobile application, it will be able to widen the non-coat to improve the performances.

• Journal of Korean Society for Geospatial Information Science
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• v.22 no.4
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• pp.107-115
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• 2014

Visibility of the road influence the safe driving because it recognizes the obstacle on the road. In this paper, we propose a mobile data acquisition and processing system for evaluating road visibility at night. And it was converted efficiently with mobile images and archived for spatial analysis of road-visibilities at night. This was applied to the following techniques to the system. Low-power computing units, open an image processing library, GPU-based acceleration techniques and document database techniques, etc. And converting the RGB image to the YUV color system, which was integrated the brightness component and the spatial information. High performance Android devices were used to collect brightness data on roads and it was confirmed whether this prototype was to determine the spatial distribution of such acquisition and management systems for spatial-assessments of road visibility at night.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.8
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• pp.1061-1067
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• 2018

In the $4^{th}$ industrial revolution of the latest, the development of the technique in the software field decides the success or failure of a country. With the trend of it, each country makes a countless effort on the education of software. This study reconstructed a questionnaire of the type of personality and conducted a research applied to learners with the help of professionals. I observed the attitude of learners during the programming class and the influence of it on the learning ability of self-oriented learning and learning motivation. As a result of it, there came up with a significant difference (at the side of self-oriented studying and the motivation of studying) depending on the type of personality of learners. Therefore, we can look forward to increasing the effects of self-oriented learning and self motivation by applying to the merits of each type of personality.

• Journal of Microbiology and Biotechnology
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• v.32 no.11
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• pp.1471-1478
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• 2022

2'-Fucosyllactose (2'-FL), the most abundant fucosylated oligosaccharide in human milk, has multiple beneficial effects on human health. However, its biosynthesis by metabolically engineered Escherichia coli is often hampered owing to the insolubility and instability of α-1,2-fucosyltransferase (the rate-limiting enzyme). In this study, we aimed to enhance 2'-FL production by increasing the expression of soluble α-1,2-fucosyltransferase from Helicobacter pylori (FucT2). Because structural information regarding FucT2 has not been unveiled, we decided to improve the expression of soluble FucT2 in E. coli via directed evolution using a protein solubility biosensor that links protein solubility to antimicrobial resistance. For such a system to be viable, the activity of kanamycin resistance protein (Kan^R) should be dependent on FucT2 solubility. Kan^R was fused to the C-terminus of mutant libraries of FucT2, which were generated using a combination of error-prone PCR and DNA shuffling. Notably, one round of the directed evolution process, which consisted of mutant library generation and selection based on kanamycin resistance, resulted in a significant increase in the expression level of soluble FucT2. As a result, a batch fermentation with the ΔL M15 pBCGW strain, expressing the FucT2 mutant (F#1-5) isolated from the first round of the directed evolution process, resulted in the production of 0.31 g/l 2'-FL with a yield of 0.22 g 2'-FL/g lactose, showing 1.72- and 1.51-fold increase in the titer and yield, respectively, compared to those of the control strain. The simple and powerful method developed in this study could be applied to enhance the solubility of other unstable enzymes.