• 제목/요약/키워드: Department of Agriculture

검색결과 11,326건 처리시간 0.039초

Clip ligation for treatment of patent ductus arteriosus occlusion in three cats

  • Ozai, Yusuke;Uemura, Akiko;Tanaka, Ryou;Takeuchi, Aki;Hamabe, Lina;Shimada, Kazumi;Yokoi, Aimi;Hirose, Miki;Watanabe, Momoko;Uehara, Katsumi
    • Journal of Veterinary Science
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    • 제23권4호
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    • pp.39.1-39.5
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    • 2022
  • Patent ductus arteriosus (PDA) is a rare congenital cardiovascular anomaly in cats. Due to their small body, intercostal thoracotomy is the most common option to close the PDA. However, few reports detail the surgical technique for ligating PDA in kittens. In this case report, three cats weighing 1.4 kg, 1.2 kg, and 2.9 kg were diagnosed PDA. Clip ligation via left fourth intercostal thoracotomy was performed and the cats were successfully treated. Postoperative echocardiography showed no residual flow in any of the cases. This case report highlights clip occlusion for small cats with PDA could be safe and effective.

한국에서 카네이션에 발생하는 Alternaria dianthi (Occurrence of Alternaria dianthi on Carnation in Korea)

  • 유승헌;강희완;이향범;김홍기
    • 한국균학회지
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    • 제17권4호
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    • pp.229-232
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    • 1989
  • Alternaria dianthi에 의한 카네이션의 검은무늬병이 1987년과 1988년 남부지방의 온실과 비닐하우스단지에서 큰 피해를 주고 있음이 관찰되었다. 심하게 감염된 재배상(栽培床)에서는 많은 카네이션 식물체가 고사(枯死)되었다. 배지상(培地上)에서의 이 균의 생육 적온은 $24-28^{\circ}C$였다. 접종실험에서 A. dianthi는 카네이션의 잎과 줄기에 병반을 형성하였다.

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Skeletal Ryanodine Receptor 1-Heterozygous PSE (Pale, Soft and Exudative) Meat Contains a Higher Concentration of Myoglobin than Genetically Normal PSE Meat in Pigs

  • Obi, T.;Matsumoto, M.;Miyazaki, K.;Kitsutaka, K.;Tamaki, M.;Takase, K.;Miyamoto, A.;Oka, T.;Kawamoto, Y.;Nakada, T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권9호
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    • pp.1244-1249
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    • 2010
  • Comparisons of properties between skeletal ryanodine receptor 1 (sRyR1)-heterozygous-mutated and normal types of meat were carried out in pigs using PSE (pale, soft and exudative) meat found during the butchering process. All samples considered to be PSE meat showed irregular running and disorder of the muscle fibers and a wider inter-fiber space upon light microscopic observation. Electron microscopy revealed disintegration, twisting, and disorder of the myofibril arrangement and elimination of the Z line in PSE meat, compared with normal meat. Meat property tests demonstrated greater decreases in water holding capacity, moisture and sarcoplasmic protein, and higher $L^*$ values for the meat color index in PSE meat than in normal meat, but there were no differences in these factors between genetically normal and sRyR1-heterozygous PSE meat. On the other hand, higher $a^*$ and $b^*$ values were observed in sRyR1-heterozygous than in normal PSE meat, and similar alterations to the a* value were observed in terms of the amount of myoglobin and density of the 17-kDa protein band, corresponding to the molecular mass of myoglobin, on SDS-PAGE gels. These results suggest that sRyR1-heterozygous PSE pork contains much more myoglobin than genetically normal PSE meat.

Efficacy of Synthesized NO-releasing Nanoparticles on the Germination and Growth of Arabidopsis thaliana

  • Nusrat Jahan Methela;Anjali Pande;Waqas Rahim;Da-Sol Lee;Bong-Gyu Mun;Geun-Mo Lee;Cho Jun-ho;Tiba Nazar Ibrahim Al Azzawi;Hak-Yoon Kim;Byung-Wook Yun
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.159-159
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    • 2022
  • Nitric oxide (NO) is a versatile signaling molecule, which is not only involved in plant growth and development but also regulates biological processes in response to biotic and abiotic stresses. Exogenous application of NO regulates the endogenous level of nitric oxide in response to stress conditions and therefore, NO donors are frequently used for stress alleviation. However, NO has very short half-life along with high reactivity. Therefore, conventional NO donors are often disadvantageous due to the relative instability of NO. On the contrary, development of NO releasing nanoparticles is a potential technique for enhancing the availability of NO in plants. Therefore, our aim was to synthesize such potential NO releasing nanoparticles which may be useful for application in agriculture. We have prepared Chitosan encapsulated S-nitrosoglutathione nanoparticles (GSNONP) and tried it with different concentrations for basic research in Arabidopsis thaliana. Our results suggest that lower concentration of this nanoparticle is highly effective for better growth of plants whereas higher concentration produces toxicity that leads to plant death. We observed better growth of Arabidopsis thaliana at 1µM concentration of the GSNONP compared to free GSNO.

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In Vitro Maturation of Tiger Oocytes: Case Report

  • Lee, Hyosang;Yin, Xijun;Lee, Youngho;Jeon, Sejin;Suh, Yongil;Jo, Sujin;Choi, Eugene;Min, Wongi;Oh, Donghan;Kong, Ilkeun
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.200-200
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    • 2004
  • The purpose of this study was carried out to determine the possibility of in vitro maturation of tiger oocytes. Immature oocytes were recovered from a pairs of ovaries. A total of 78 oocytes were collected, of which forty threes were identified as compact cumulus cells and uniform cytoplasm. 43 COCs were in vitro matured at 39℃, 5% CO₂ in air atmosphere for 48 h in a IVM medium (TCM-199 supplement with 10% FBS, 0.6 mM cysteine, 0.2 mM pyruvic acid and 10 IU/㎖ HMG). (omitted)

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Soil Application of Metarhizium anisopliae JEF-314 Granules to Control, Flower Chafer Beetle, Protaetia brevitarsis seulensis

  • Kim, Sihyeon;Kim, Jong Cheol;Lee, Se Jin;Lee, Mi Rong;Park, So Eun;Li, Dongwei;Baek, Sehyeon;Shin, Tae Young;Gasmi, Laila;Kim, Jae Su
    • Mycobiology
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    • 제48권2호
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    • pp.139-147
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    • 2020
  • Root-feeding Scarabaeidae, particularly white grubs are considered among the most harmful coleopteran insect pests in turfgrass. In this work, sixteen entomopathogenic fungal species were assayed against flower chafer beetle, Protaetia brevitarsis (Coleoptera: Scarabaeidae) and Metarhizium anisopliae JEF-314 showed high virulence. The control ability of the isolate JEF-314 has been in detail tested for a model insect flower chafer beetle. Further analyses showed insect stage-dependent virulence where the fungal virulence was the highest against smaller instar larvae. Additionally, we confirmed that millet-based solid cultured granule was effective against the soil-dwelling larval stage. The isolate also showed a similar ability for a representative pest (Popillia spp.) in laboratory conditions. Our results clearly suggest a high potential of M. anisopliae JEF-314 to control the flower chafer beetle, possibly resulting in controlling of root-feeding white grubs in turfgrass. Based on the insect life cycle and susceptibility to the fungus, late spring and summer time would be the optimum time to apply JEF-314 granules for an effective control. Further characterization of the efficacy of the fungus under field conditions against the Scarabaeidae beetles might provide an efficient tool to control this beetle in an environment-friendly way.

Effects of Synchronization of Carbohydrate and Protein Supply in Total Mixed Ration with Korean Rice Wine Residue on Ruminal Fermentation, Nitrogen Metabolism and Microbial Protein Synthesis in Holstein Steers

  • Piao, Min Yu;Kim, Hyun-J.;Seo, J.K.;Park, T.S.;Yoon, J.S.;Kim, K.H.;Ha, Jong-K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권11호
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    • pp.1568-1574
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    • 2012
  • Three Holstein steers in the growing phase, each with a ruminal cannula, were used to test the hypothesis that the synchronization of the hourly rate of carbohydrate and nitrogen (N) released in the rumen would increase the amount of retained nitrogen for growth and thus improve the efficiency of microbial protein synthesis (EMPS). In Experiment 1, in situ degradability coefficients of carbohydrate and N in feeds including Korean rice wine residue (RWR) were determined. In Experiment 2, three total mixed ration (TMR) diets having different rates of carbohydrate and N release in the rumen were formulated using the in situ degradability of the feeds. All diets were made to contain similar contents of crude protein (CP) and neutral detergent fiber (NDF) but varied in their hourly pattern of nutrient release. The synchrony index of the three TMRs was 0.51 (LS), 0.77 (MS) and 0.95 (HS), respectively. The diets were fed at a restricted level (2% of the animal's body weight) in a $3{\times}3$ Latin-square design. Synchronizing the hourly supply of energy and N in the rumen did not significantly alter the digestibility of dry matter, organic matter, crude protein, NDF or acid detergent fiber (ADF) (p>0.05). The ruminal $NH_3$-N content of the LS group at three hours after feeding was significantly higher (p<0.05) than that of the other groups; however, the mean values of ruminal $NH_3$-N, pH and VFA concentration among the three groups were not significantly different (p>0.05). In addition, the purine derivative (PD) excretion in urine and microbial-N production (MN) among the three groups were not significantly different (p>0.05). In conclusion, synchronizing dietary energy and N supply to the rumen did not have a major effect on nutrient digestion or microbial protein synthesis (MPS) in Holstein steers.

Structure Analysis of the Full Length PDI Genomic DNA Isolated from Bombyx mori

  • Kim, Sung-Wan;Goo, Tae-Won;Yun, Eun-Young;Park, Kwang-Ho;Hwang, Jae-Sam;Kang, Seok-Woo;Kwon, O-Yu
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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    • pp.44-44
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    • 2003
  • Protein disulfide isomerase (PDI) is not only an isomerase catalyzing the formation of native disulfide bond(s) of nascent peptide, but also a molecular chaperone assisting chain folding. We have already reported the structure of a cDNA (bPDl) encoding PDI from Bombyx mori and the function of PDI as foldase in assisting protein folding. (omitted)

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Preparation and Characterization of Hydrophobic Coatings from Carnauba Wax/Lignin Blends

  • BANG, Junsik;KIM, Jungkyu;KIM, YunJin;OH, Jung-Kwon;YEO, wanmyeong;KWAK, Hyo Won
    • Journal of the Korean Wood Science and Technology
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    • 제50권3호
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    • pp.149-158
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    • 2022
  • To realize the infinite possibilities of materials derived from wood, it is necessary to overcome the weak moisture stability of wood. Thus, the development of an eco-friendly hydrophobic coating agent is essential, and of these, woody biomass-based materials are strongly attractive as coatings. In this study, eco-friendly hydrophobic wood coatings were prepared using carnauba wax purified from palm leaves and sprouts, and kraft lignin. The physicochemical properties of the carnauba wax/lignin blends according to the ratio of carnauba wax and lignin were observed by morphology and functional group change. In addition, the coating performance of carnauba wax/lignin blend coatings was confirmed by measuring the contact angle change. It was found that the addition of lignin could accelerate the atomization of wax particles, and that micro-roughness can be realized when applied to the actual wood surface, to ensure that the coating effect over time lasts longer. In addition, it was confirmed that the addition of lignin increases the hydrogen-bond-based interaction with the wood of the coating, thereby providing better coating stability and increasing the durability of the coating solvent under friction. The carnauba wax/lignin paint developed in this way is eco-friendly because all components are made of wood-based raw materials and have an excellent affinity with wood surfaces. Therefore, it is expected to be applicable to the coating process of wood-plastic composites and timber composites.