Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
- /
- Pages.200-200
- /
- 2004
In Vitro Maturation of Tiger Oocytes: Case Report
- Lee, Hyosang (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Yin, Xijun (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Lee, Youngho (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Jeon, Sejin (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Suh, Yongil (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Jo, Sujin (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Choi, Eugene (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Min, Wongi (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Oh, Donghan (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Kong, Ilkeun (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University)
- Published : 2004.06.01
Abstract
The purpose of this study was carried out to determine the possibility of in vitro maturation of tiger oocytes. Immature oocytes were recovered from a pairs of ovaries. A total of 78 oocytes were collected, of which forty threes were identified as compact cumulus cells and uniform cytoplasm. 43 COCs were in vitro matured at 39℃, 5% CO₂ in air atmosphere for 48 h in a IVM medium (TCM-199 supplement with 10% FBS, 0.6 mM cysteine, 0.2 mM pyruvic acid and 10 IU/㎖ HMG). (omitted)