• Journal of Veterinary Science
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• 제22권6호
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• pp.76.1-76.15
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• 2021

Background: The development of a vaccine for Jembrana disease is needed to prevent losses in Indonesia's Bali cattle industry. A DNA vaccine model (pEGFP-C1-tat) that requires a functional delivery system will be developed. Polylactic-co-glycolic acid (PLGA) may have potential as a delivery system for the vaccine model. Objectives: This study aims to evaluate the in vitro potential of PLGA as a delivery system for pEGFP-C1-tat. Methods: Consensus and codon optimization for the tat gene was completed using a bioinformatic method, and the product was inserted into a pEGFP-C1 vector. Cloning of the pEGFP-C1-tat was successfully performed, and polymerase chain reaction (PCR) and restriction analysis confirmed DNA isolation. PLGA-pEGFP-C1-tat solutions were prepared for encapsulated formulation testing, physicochemical characterization, stability testing with DNase I, and cytotoxicity testing. The PLGA-pEGFP-C1-tat solutions were transfected in HeLa cells, and gene expression was observed by fluorescent microscopy and real-time PCR. Results: The successful acquisition of transformant bacteria was confirmed by PCR. The PLGA:DNA:polyvinyl alcohol ratio formulation with optimal encapsulation was 4%:0.5%:2%, physicochemical characterization of PLGA revealed a polydispersity index value of 0.246, a particle size of 925 nm, and a zeta potential value of -2.31 mV. PLGA succeeded in protecting pEGFP-C1-tat from enzymatic degradation, and the percentage viability from the cytotoxicity test of PLGA-pEGFP-C1-tat was 98.03%. The PLGA-pEGFP-C1-tat demonstrated luminescence of the EGFP-tat fusion protein and mRNA transcription was detected. Conclusions: PLGA has good potential as a delivery system for pEGFP-C1-tat.

• Journal of information and communication convergence engineering
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• 제2권2호
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• pp.116-118
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• 2004

In the power industry after restructuring of Power industry will be appeared on-site type business, power retail sales business, and power wholesales business, power dealing business, customer inclination business ＆ delivery of power facilities. Among them, power trade business, customer inclination business and on-site type business will be rapidly increased and occupied attention. In addition, it is forecasted to advent the broker, provider, market place, power marketer, system operator and generator as a main player. Meanwhile, it needs protection of existing power industry and activation of new energy market for accomplishment of restructuring of power industry.

• 정보학연구
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• 제4권2호
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• pp.83-102
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• 2001

본 연구에서는 최근 들어 인터넷 상거래 물량과 업체의 증가에 따라 그 중요성이 더해가고 있는 택배운송업의 본질을 규명하고 국내외 업체의 사업현황을 개괄적으로 살펴본 다음, 해외 선진택배업체의 전략적 특징과 국내 택배업의 전자상거래 변화추세 및 전자상거래 배송현황을 고찰을 통해 전자상거래시대에 국내 택배업이 지니는 문제점과 전자상거래 물류전략을 구체적으로 지적함으로써 전자상거래 시대에 국내 택배운송업체들의 경쟁력강화에 일조하고자 한다.

• 한국산림과학회지
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• 제90권3호
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• pp.355-362
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• 2001

이 연구는 우리나라 제지산업의 경쟁력을 평가하기 위하여 경쟁력 결정요인을 분석하여 제지산업 발전을 위한 경쟁력 강화방안을 제시하는 것을 목적으로 수행되었다. 우리나라 제지산업의 국제경쟁력 평가를 위해서 제지산업 전문가를 대상으로 한 델파이 조사를 이용하였다. 제지산업 국제경쟁력 평가는 내수시장 및 수출시장으로 구분하여 지종별로 경쟁력 결정요인을 선정하였다. 우리나라 제지산업의 국제경쟁력을 결정짓는 요인은 품질, 원가, 물류, 납기, 원료, 마케팅, 기타의 순서로 중요성이 인식되었다. 이중 우리나라는 국내 및 수출시장에서 경쟁국과 비교할 때 품질과 납기는 경쟁력이 있으나 원료와 제조원가에 있어서는 경쟁력이 없는 것으로 밝혀졌다.

• 한국BIM학회 논문집
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• 제5권3호
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• pp.11-18
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• 2015

Integrated Project Delivery (IPD) is a new trend in the building industry and a key feature of IPD is the use of BIM to support project collaboration. Therefore, engineers should learn the use of BIM and IPD in the construction industry. In this study, the authors draw necessary components for developing an IPD course curriculum through analyzing a project case and current courses provided by universities in Korea. The authors make several suggestions for effective IPD education in universities.

• 한국건설관리학회논문집
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• 제12권5호
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• pp.81-92
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• 2011

위험형 CM(CM at Risk) 제도의 시행과 함께 토목시설물에도 CM계약사례가 증가할 것으로 예상되어, 국토해양부 등에서는 CM 업무지침을 공표하고 있다. 현재 공표된 CM 업무지침은 CM 계약 이후의 업무지침이지만, CM 발주사례가 부족한 토목사설을 포함한 공공사업에서는 CM 발주 자체에 필요한 CM발주의 가획단계부터 CM 업자 (Construction Manager, CMr) 선정까지의 업무지침이 필요한 실정이다. 따라서 본 연구에서는 발주처의 건설사업관리 발주업무를 발주 기획단계부터 CMr 선정단계까지 표준화된 프로세스 모형으로 구축하였으며, 이를 위해 CM발주 업무분류체계를 구성하였다. 또한 연구에서는 구축된 모형에 근거한 CM 발주지원 시스템을 개발하여 시스템의 실무 운영성을 검증하였다. 이러한 연구는 CM 발주사례가 부족한 발주처의 CM 발주업무 지원체계로 활용성을 가질 수 있다.

• 복식
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• 제38권
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• pp.245-260
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• 1998

Our country's fur clothing industry has rapidly grown for a short time since it started with simple needlework of fur pieces, and now it is still developing as export industry that some enterprises besides general business firm export more than ten million dollars a year. However, it is true that fur clothing industry is still in unsatisfactory level in its size and system, compared with manufacturing or leather industry. Of course, we import crude material and processed fur on an entire base, and we have few wild fur on an entire base, and we have few wild fur animals because of our natural condition and it is hard to breed them. On the other hand, the world's fur industry shows that the demand of fur clothes is increasing as fur animal breeding is improved and developed and supply has become rather abundant with the help of fur clothing fashion since late 19th century : Also, there is a trend that some developed countries depend on importing rather than self-prodoction because of the character-istic of labor-concetntraled fur industry. Accordingly, considering this situation, we can expect fur clothing industry as promising export industry by analyzing the present situation of our country's fur clothing industry and suggesting its problems and solutions, and here are developing plans for growing fur clothing industry as promising export industry. First, smoothing delivery of crude material. Second, making high quality products and diversifying goods. Third, establishing effective competition system in fur clothing industry. Fourth, raising skilled man power. Lastly, there should be coopetation among the related fields in the aspect of policy including the support from the government.

• 농업과학연구
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• 제43권3호
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• pp.369-378
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• 2016

Improving the reproductive traits of Hanwoo might decrease their production cost. Therefore, this study was conducted to investigate the effects of environmental factors [registration grade (basic, pedigree or advanced), birth year, birth season, parity, delivery year, and delivery season] on various reproductive traits (age at 1st service, age at 1st conception, age for 1st calving, days at 1st service postpartum, non-pregnant condition period, calving interval, gestation length, and number of services for conception) in Hanwoo (primiparous 12,219 heads, multiparous 10,471 heads). All data was acquired from Gyeongnam province areas which were surveyed from 2007 to 2015. All environmental factors significantly influenced (p < 0.01) reproductive traits of primiparous cows but, but not all environmental factors influenced multiparous cows. Primiparous cows registered as advanced grade showed significantly lower age at 1st service (by 15.36 days), age at 1st conception (by 8.66 days), and age for 1st calving (by 8.77 days) (p < 0.01) than those registered as basic grades. Age at 1st service, age at 1st conception and age for 1st calving were not significantly related to birth year in primiparous cows. As delivery years advanced from 2005 to 2012, all durations associated to reproductive traits tended to be shorter. Days at 1st service postpartum, non-pregnant condition period, and calving interval tended to be shortened as parity increased. Days at 1st service postpartum, days open, calving interval, and gestation in multiparous cows calved in winter were shorter than those in summer. The registration grade was not a effected with reproductive traits in Hanwoo.

• 한국IT서비스학회지
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• 제10권3호
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• pp.285-296
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• 2011

Recently world industry trends is moved from manufacturing to service. As a result product-market ideology is central to the service enabler of the service. And value creation and delivery of consumer-oriented services as the core design industry is gaining popularity abroad. But the Service design to dispose of the whole process from planning to be done systematically is not yet established a clear concept. This study suggested problems of the current service design industry, service plans and strategies for the development of service design through open questionnaire and FGI(Focus Group Interview) for using service model methodology which is presented by HyeogIn Kwon et al.(2010). And we also consider 27 kinds of developing challenges for service design industry innovation by 6 sectors, such as workforce, R&D, legal framework, lay the foundation, knowledge service and collaborative service.

• 벤처창업연구
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• 제16권5호
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• pp.91-107
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• 2021

본 연구는 점점 경쟁이 심해지고 있는 국내 프랜차이즈 산업에서 최근 10여 년간 급성장해 온 커피전문점의 수익성에 영향을 미치는 요인이 무엇인지 살펴보고, 사전 창업교육이 커피전문점 수익성에 영향을 미치는 요인에 어떤 영향을 미치는 지를 분석하였다. 창업교육을 통해 점포의 업종 경쟁력을 높일 수 있는지, 입지를 선정하는 안목을 키울 수 있는지, 혁신경영이 일어나는지를 분석하였고, 궁극적으로 점포 수익성으로 귀결되는지 구조방정식 모형으로 분석하였다. 분석 결과, 창업교육은 점포의 업종특성인 맛과 품질, 가격적절성, 메뉴다양성, 점포분위기 등과 점포의 입지특성인 적합성과 접근성, 그리고 점포의 혁신경영인 배달영업과 SNS마케팅 활용 등에 모두 유의한 정(+)의 영향을 미치는 것으로 나타났다. 점포수익성에 미치는 영향은 업종특성 중 맛과 품질과 가격적절성, 입지특성 중 적합성, 혁신경영 중 SNS마케팅 활용 요인만이 유의한 정(+)의 영향을 미치는 것으로 나타났다. 본 연구는 과당경쟁을 하고 있는 커피전문점의 수익성 제고를 위해 실무적 시사점을 제시하였고, 가맹본부 및 정부정책의 유용한 자료로도 활용될 수 있을 것으로 기대된다. 아울러 본 연구의 결과를 통해 향후 가맹본부와 정부 및 지방자치단체는 증가하고 있는 자영업자의 배달영업이 배달비용을 감안하더라도 실질적 수익성 개선으로 이어지도록 제도적 뒷받침이 필요하다는 시사점이 제시되었다.