• 대한한의학방제학회지
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• 제5권1호
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• pp.127-145
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• 1997

This study was aimed to investigate the anti-tumor and immune response effect of Samyongtang(S1; this medicine represents for 'ENERGIZER'), Yangjeongjejeoktang(S2; this is the 'INTERMEDIATE METHOD' of S1 and S3) and Onbeakwon(S3; this is 'ATTACK' the disease of mass) on the experimental rats induced by Sarcoma-180 and Methotrexate. And to observed the differences S1, S2, and S3 treatment groups. Tumor weight(TW) in vivo, interleukin-2(IL-2), hemagglutinin titer(H.A), hemolysin titer(HL), rossete forming cell(RFC), delayed type hypersensitivity(DTH), and natural killer cell activity(NKCA) in vivo were measured in rats. The obtained results were summarized as follows. 1. Tumor weight was decreased in all treatment groups (S2>S3>S1) as compared with control group, but the difference was not statistically significant each treatment groups. 2. Interleukin-2 was increased in all treatment groups (S1>S2>Ss) as compared with control group, but the difference was not statistically significant each treatment groups. 3. Hemagglutinin titer was increased in all treatment groups (S1>S2>S3) as compared with control group, but the difference was not statistically significant each treatment groups. 4. Hemolysin titer was increased in all treatment groups (S1>S2>S3) as compared with control group, but the difference was not statistically significant each treatment groups. 5. Rossete forming cell(RFC) was increased in all treatment groups (S1>S2>S3) as compared with control group, but the difference was not statistically significant each treatment groups. 6. Delayed type hyperseneitivity(DTH) was increased in S1, S2 treatment groups (S1>S2) as compared with control group, but it was decreased in S3 treatment group. Therefore, S1 group was statistically significant compared with S3 groups. 7. Natural killer cell activity(NKCA) was increased in all treatment groups (S2>S3>S1) as compared with control group, but the difference was not statistically significant each treatment groups. Based on the above mentioned results, it is suggested that S1, S2 and S3 will have anti-tumor substances and enhance effect of immune response. But the differences were not statistically significant in each treatment groups, except for delayed type hypersensitivity.

• 대한미생물학회지
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• 제18권1호
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• pp.91-98
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• 1983

The effect of subcutanecus injection of Corynebacterium parvum($700{\mu}g$) on cellular and humoral immune responses when given at various time relative to sheep red blood cell(SRBC) sensitization were studied by the evaluation of Arthus, delayed-type hypersensitivity(DTH), rosette forming cell, hemagglutinin and hemolysin reactions. Arthus reactivity(3 hours) developed in control mice and test mice pretreated with C. parvum 8 days prior to intravenous sensitization with SRBC were similar. However, there was slight depression of reactivity when C. parvum was given subcutaneoutly(s.c.) 4 or 2 days prior to SRBC sensitization. Arthus reactivity was significantly depressed when C. parvum was given s.c. either at the same time as, or 2 days later than, antigen. DTH reaction was net depressed significantly when C. parvum was injected 8 or 2 days prior to SRBC sensitization or at the same time as antigen. In contrast DTH was significantly augmented when C. parvum given s.c. 4 days prier to SRBC sensitization. DTH was depressed when C. parvum was given s.c. 2 days after antigen. No significant change occurred in rosette forming percetages of spleen cell when C. parvum was given s.c. 8, 4 or 2 days before SRBC sensitization. In contrast, a significant reduction in percentages of rosette forming cell occurred when C. parvum was given s.c. either at the same time as, or 2 days later than, antigen. Serum hemaggulutinin and hemolysin titers were not significantly affected by subcutaneous injection of C. parvum regardless of time relative to SRBC sensitization. However, mercaptoethanol-resistant hemaggulutinin and hemolysin(IgG) titers were somewhat augmented when C. parvum was given 2 days after antigen. It is concluded from these results that depending on the time and route of inoculation, C. parvum can enhance or depress immune responses in mice, suggesting the time and route of C. parvum inoculation is an important point of concern about clinical use of C. parvum for the treatment of cancer.

• 대한한방소아과학회지
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• 제8권1호
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• pp.1-12
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• 1994

In order to investigate the effects of Insamyangwee Tang on cell-mediated and humoral immune response, solid extract of Insamyangwee Tang (sample A), mixture of individual solid extract of Insamyangwee Tang (sample B) were administered orally for 14 days. The auther used ICR mice having a body weight of about 20-22g as experimental animals dividing them into three groups-Saline, Sample A and Sample B group. All of the mice were sensitized i.v. with $10^8$ sheep red blood cells(SRBC) and challenged i.d. with $10^8$ SRBC 4 days later. Such immune responses as delayed-type hypersensitivity(DTH), rosette forming cells(RFC), hemagglutinin titers(HA titers) and hemolysin titers(HL titers) were measured at 24 hours after challenge. The results were as follow: 1. DTH in Sample A & Sample B group was increased, as compared with Saline group, with satistical significance. 2. RFC in Sample A & Sample B group were increased, as compared with Saline group, with statistical significance. 3. HA titers in Sample A & Sample B group were not increased, as compared with Saline group, with statistical significance. 4. HL titers were increased just only in Sample A group with statistical significance. The inference from the above results is that Sample A group is better than Sample B group, and Insamyangwee Tang enhance the cell-mediated and humoral immune response.

• 대한수의학회지
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• 제26권1호
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• pp.109-115
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• 1986

Experiments were performed on mice to investigate the effects of a polycyclic aromatic hydrocarbon, 3-methylcholanthrene (MCA) on Arthus reaction, delayed-type hypersensitivity (DTH) and antibody production to sheep red blood cells (SRBC). Mice were sensitized iv with 0.1ml of 1% SRBC suspension were treated with a single ip injection of olive oil alone or with different doses of MCA in oil (0.5~50mg/Kg) at various time before (-) or after (+) sensitization (day 0) and were challenged at 4 days after SRBC. Arthus reaction was measured at 3 hours after challenge and other responses at 24 hours. Treatment with MCA inhibited Arthus reaction and DTH to SRBC, measured by footpad swelling reaction, and this immunosuppressing effect was dependent on the dose and time of MCA treatment in relation to SRBC sensitization. Humoral immune responses as measured by serum hemagglutinin-and hemolysin-titers to SRBC were significantly depressed when MCA was injected before or at the same time of sensitization. However, the response was slightly depressed when injected after SRBC. These results indicate that MCA suppress the function of the cells involved in immune responses.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.610-614
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• 1998

DW-116, [1-(5-fluoro-2-pyridyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-1,4-dihydro-4-oxoquino-line-3-carboxylic acid hydrochloride}, is a new quinolone antibiotic with a broad antibacterial spectrum against G(+) and G(-) bacteria. DW-116 was evaluated for the immunomodulating activities, which is one of the efforts to investigate the mechanism of action related to the good in vivo antibacterial efficacy. The results of in vitro studies revealed there was no statistically significant increase in B and T lymphocyte proliferation. But the results of in vivo studies showed that the number of plaque forming cells (PFC), the amount of polyclonal antibodies and delayed-type hypersensitivity (DTH) were significantly increased after the repeat administration with 12 and 60 mg/kg of DW-116. Taken together, these results proposed that immunostimulting effect of DW-116 could be one of the action mechanisms for demonstrating in vivo antibacterial activities under these experimental conditions.

• 대한약침학회지
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• 제21권2호
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• pp.90-97
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• 2018

Silymarin is a flavonoid complex extracted from the Silybum marianum plant with a wide range of pharmacological and biochemical effects. In the present study, the immunomodulatory effects of silymarin were investigated in BALB/c mice. Silymarin was administered daily by intraperitoneal injection at doses of 50, 100 and 150 mg/kg for 14 consecutive days. Following the exposure, host hematological parameters, spleen cellularity and histopathological examination, as well as delayed-type hypersensitivity (DTH) responses, hemagglutination titers (HA), splenocyte cytokine production and lymphocyte proliferation assay were studied in all of the test groups of animals. The results showed that the low dose of silymarin (50 mg/kg) could stimulate both cellular and humoral immune functions in the treated hosts. In addition, silymarin at 100 mg/kg appeared to impact on DTH responses and lymphoproliferation. Based on the finding here, it would seem that silymarin has efficient immunostimulant properties. As a recommendation, the application of silymarin along with acupuncture technique (herbal acupuncture) can be thought as a good plan to modulate and enhance the immune system for the management of several immunodeficiency disorders. However, further studies are required to demonstrate this hypothesis.

• 사상체질의학회지
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• 제1권1호
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• pp.125-138
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• 1989

In order to investigate the effect of Row ginseng (Ra. G.; from $K{\acute{u}}msan$ province, Korea), White ginseng (W.G.; from $K{\acute{u}}msan$ province, Korea), and Red ginseng (Re. G.; form $K{\acute{u}}msan$ province, Korea) on immune response, the author used ICR mice having a body weight of about 20g as experimental animals dividing them into four groups-Saline, Ra. G, W.G., Re. G group. Delayed type hypersensitivity(DTH) and rosette forming cells(RFC) for cell-mediated immune response, hemagglutinin(HA) titers, hemolysin (HL) titers for humoral immune response were measured at 24 hours after challenge, The results were summarized as follows: 1) DTH was increased in all of the treated group as compared with the Saline group, with statistical significance(W.G.> Re. G. > Ra. G.). 2) RFC was increased in all of the treated group as compared with the Saline group, with statistical significance(W.G. > Re. G. > Ra. G.). 3) HA titer was increased in all of the treated group as compared with the Saline group, with statistical significance (Re. G.> W. G.> Ra. G.). 4) HL titer was increased in all of the treated group as compared with the Saline group, with statistical significance (Re. G. > W. G.> Ra. G.). Through the experimental study in ICR mice, these findings suggest that Raw ginseng, White ginseng and Red ginseng enhance both cell-mediated and humoral immune response with statistical significance.

• 대한미생물학회지
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• 제16권1호
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• pp.49-55
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• 1981

최근(最近) 히스타민이 면역반응(免疫反應)을 조절(調節)함이 구명(究明)되고 있으나 생체내(生體內) 실험(實驗) 특(特)히 마우스에서의 연구보고(硏究報告)를 희소(稀少)하다. 본(本) 실험(實驗)에서는 histamine-2-receptor antagonist($H_2$ 차단제(遮斷劑))인 cimetidine과 히스타민이 마우스의 면양적혈구(緬羊赤血球)(SRBC)에 대(對)한 면역반응(免疫反應)에 미치는 영향(影響)을 실험(實驗)하였다. 마우스를 매일(每日) 14일간(日間) 여러가지 농도(濃度)의 cimetidine 으로 전처리(前處理)하고 여러가지 농도(濃度)의 SRBC($10^6,\;10^7$ 및 $10^8$ 세포(細胞))로 면역(免疫)하고 4일(日) 후(後)에 마우스 족척(足蹠)에 SRBC로 야기주사(惹起注射)하여 Arthus반응(反應)과 지연성과민반응(遲延性過敏反應)(DTH)를 족척종창반응(足蹠腫脹反應)으로 측정(測定)하였으며, 체액성면역반응(體液性免疫反應)은 적혈구응집소가(赤血球凝集素價)를 측정(測定)하였다. 수(數) 종(種) 농도(濃度)($10^{-1}M,\;10^{-3}M$, 및 $10^{-5}M$)의 히스타민을 야기주사(惹起注射)와 동시(同時)에 주사(注射)하여 24시간(時間)-DTH를 측정(測定)하여 히스타민 효과(效果)를 평가(評價)하였다. Cimetidine은 DTH를 항진(亢進)시켰으며 그 항진(亢進)은 250 ${\mu}g$의 cimetidine을 투여(投與)하였을 때 현저(顯著)하였다. 그러나 Arthus 반응(反應)과 혈청항체가(血淸抗體價)는 cimetidine 전처리(前處理) 군(群)과 대조군간(對照群間)에 유의(有意)한 차이(差異)가 없었다. 히스타민은 SRBC에 대(對)한 DTH를 투여량-의존성(投與量-依存性) 유형(類型)으로 억제(抑側)하였으며 그 억제(抑制)는 저농도(低濃度)의 항원량(抗原量)($10^6$ 및 $10^7$ SRBC)일때 더 현저(顯著)하였다. 그러나 외인성(外因性) 히스타민은 $10^8$ SRBC로 면역(免疫)하였을 때늘 DTH를 감소(滅少)시키지 않았다. 이상(以上)의 본(本) 실험결과(實驗結果)는 cimetidine이 세포성(細胞性) 면역반응(免疫反應)을 항진(亢進)시키나 체액성(體液性) 면역반응(免疫反應)은 증가(增加)시키지 않으며 내인성(內因性) 및 외인성(外因性) 히스타민 즉시형과민반응(卽時型過敏反應)뿐만 아니라 세포성(細胞性) 면역반응(免疫反應) 조절(調節)에 관여(關與)함을 강력(强力)히 시사(示唆)하는 증거(證據)라고 사료(思料)되었다.

• Environmental Analysis Health and Toxicology
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• 제3권1_2호
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• pp.9-19
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• 1988

The effect of rancid perilla oil on the immune response in mice was studied. ICR male mice were divided into 5 groups and were fed on the experimental diets for 4 weeks. Mice were sensitized and challenged with sheep red blood cell. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette forming cell and macrophage activity. Biochemical items were measured by serum protein and serum albumin. The weight of spleen, thymus and liver were measured. The rancid perilla oil diets decreased humoral and cellular immune responses, the number of peripheral circulating white blood cells and total protein and serum albumin. These results showed that the high rancid perilla oil diet decreased more humoral and cellular immune response, the number of peripheral circulating white blood cells, and total protein and serum albumin than the low rancid perilla oil diet did.

• Environmental Analysis Health and Toxicology
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• 제1권1호
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• pp.37-46
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• 1986

Experiments were performed to investigate the effect of Panax ginseng petroleum ether fraction on delayed type hypersensitivity, rosette formation, phagocytic activity and histophathological influence in lead acetate treated mice. Lead acetate was administered in the drinking water and ginseng pet. ether fraction was injected i.p.. Mice were sensitized and challenged with sheep red blood cells. Erythrocyte(I) rosette formation and DTH reaction were significantly depressed in lead acetate treated mice, and those were restored administration of ginseng fraction. Ginseng pet. ether fraction administration did not have any effect on decreased phagocytic activity. Follicular and parafollicular areal destruction of spleen, and destruction of thymus were finded in lead acetate exposed-mice. Small dose of ginseng pet. ether fraction (5 mg/kg, 10 mg/kg), administraction inhibited those histopathological changes, but large dose (20 mg/kg) didn't.