• Archives of Pharmacal Research
• /
• v.23 no.6
• /
• pp.626-632
• /
• 2000

Brazilin, an active principle of Caesalprenia sappan, was examined for its immunopotentiating effects in multiple low dose streptozotocin (MLD-STZ) induced type diabetic mice. Brazilin was intraperitoneally administered for 5 consecutive days to MLD-STZ induced type 1 diabetic mice. Delayed type hypersensitivity, Con A-induced proliferation of splenocytes and mixed lymphocyte reaction, which had been decreased in diabetic mice, were significantly recovered by the administration of brazilin. Brazilin increased IL-2 production without affecting suppressor cell activity. Con A-induced and IL-2-induced expression of high affinity IL-2 receptors were also enhanced by brazilin. These results indicate that brazilin augments cellular immune responses, which are suppressed in the MLD-STZ induced type I diabetic mice, by increasing IL-2 production and responsiveness of immune cells to IL-2.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.17 no.1
• /
• pp.123-129
• /
• 2003

Experimental studies were done to research the dinical effects of Juglandis Semen aqua-acupuncture and acupuncture(BL 13) on the anti-allergic response. anaphylaxis provoked by the compound 48/80, delayed type hypersensitivity response to picryl chloride and SASC and inflammation response to egg albumin. The following results have been obtained: Juglandis Semen aqua-acupuncture and acupuncture(BL13) group were increased the survival rate in compound48/80 induced systemic anaphylactic reaction. Picryl chloride induced contact dermatitis and delayed type hypersensitivity in SRBC challenged mouse were significantly decreased in Juglandis Semen aqua-acupuncture and acupuncture (BL13) group. Inflammation response - WBC, CRP and Nitric Oxide in egg albumin induced allergic rat were significantly decreased in Juglandis Semen aqua-acupuncture and acupuncture(BL13) group. According to the above results, Semen aqua-acupuncture and acupuncture(BL13) both depress the allergy reaction.

• Environmental Analysis Health and Toxicology
• /
• v.2 no.1_2
• /
• pp.33-42
• /
• 1987

Experiments were performed to investigate effects of ethanol and saccharin on the immune system in rats. 4% ethanol and 0.02, 0.20, 2.00% saccharin solution in 4% ethanol were provided ad libitum by tap water for 4 weeks. Rats were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by relative immuno organ weight, antibody production, Arthus reaction, delayed type hypersensitivity, and rosette forming cell. Ethanol exposure decreased thymus weight and delayed type hypersensitivity. A combined solution of ethanol and saccharin decreased water intake, growth rate, spleen weight, thymus weight, humoral and cellular immune response. Especially, a 2% saccharin solution in 4% ethanol very significantly suppressed cellular immunity.

• Toxicological Research
• /
• v.6 no.1
• /
• pp.13-19
• /
• 1990

The effects of panaxytrion as known to be a cytotoxic substance isolated from Panax ginseng on the immune responses were examined. The i.p. administration of panaxytriol to normal mice for 6 consecutive days as doses of 5, 10 and 20 mg/kg suppressed the increase of body weight dose-dependently but did not affect the weight ratio of immunoorgans to body weight, No significant changes were observed in the humoral immune responses as measured by Arthus reaction and plaque forming cells and in the cellular immune response as measured by delayed hypersensitivity as well as phagocytic activity of reticuloendotherial system. These results suggested that panaxytriol, a cytotoxic substance to cancer cells, has no detrimental effects on the immune function in normal mice.

• Korean Journal of Pharmacognosy
• /
• v.14 no.3
• /
• pp.102-106
• /
• 1983

As many crude drugs are used in the oriental medical field problems on the side effects of these drugs come to the front. To conduct delayed-type hypersensitivity we selected 29 kinds of drugs used frequently for therapeutic agents in oriental medical hospitals (Table I). The cell-mediated immune response was evaluated by measuring the foot pad swelling reaction and humoral immune response by measuring the antibody formation to these crude drugs. Mice were given these drugs intraperioneally for sensitization and challenged with same drug as used for sensitization respectively by intral dermal injection on the left and righ hind foot pad 4 days after senstization and then the foot pads were measured with the dial micrometer. The results were as follow; 1) Gentianae Scabrae Radix, Arecae Semen, Corydalis Tuber, and Paeoniae Radix were significant as delayed-type hypersensitivity inducers. 2) None of the crude drugs tested had effect on the induction of humoral immune response.

• The Journal of Pediatrics of Korean Medicine
• /
• v.5 no.1
• /
• pp.1-14
• /
• 1991

Experimental studies were done to research the clinical effects of Biahwan and Sutodan on the Anti-allergic Effect in Rats and Mice. The results were summerized as follows; 1. In the 48hours homologous passive cutaneous anaphylaxis in rats provoked by the IgE-like antibody against egg white albumin, Biahwan and Sutodan revealed significant effects. 2. In the effects of Biahwan and Sutodan on vascular permeability responses to intradermal histamine and serotonin in rats, Biahwan revealed significant effect, but Sutodan revealed none significant effect. 3. In the delayed type hypersensitivity responses to Picryl Chloride in mice, Biahwan and Sutodan revealed none significant effects. 4. In the delayed type hypersensitivity responses to SRBC (Sheep Red Blood Cell) in mice, Biahwan and Sutodan revealed significant effects.

• YAKHAK HOEJI
• /
• v.46 no.1
• /
• pp.52-57
• /
• 2002

Effects of the butanl fraction of Astragali Radix (BFAR) on the cellular and nonspecific immune responses were investigated in ICR mice. Mice were divided into 4 groups and BFAR at doses of 5, 25 and 125 mg/kg u ere administered orally to mice daily for 3 weeks, and the normal animals were given vehicle. The results of this study are summarized as follows; the relative weight of thymus was greatly increased by BFAR treatment, compared with that in mormal mice. However, the body weight gain was not affected. Delayed-type hypersensitivity (DTH) reaction to sheep red blood cells (SRBC) for cellular immunity was significantly enhanced by BFAR treatment, compared with those in normal mice. In these mice, BFAR also dose-dependently increased activities of phagocyte and natural killer (NK) cells as well as the number of leukocyte resulted from nonspecific immunity Thus, these results demonstrate that BFAF treatment results in a significant increase in both cellular and nonspecific immune responses to antigen in concentrations that enhance humoral immune function.

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.194.2-195
• /
• 2003

Phenylpropanoids(PP), C6-C3 compounds, are widely distributed in many plants. In this experiments, effect of PP on sheep red bood cells (sRBC)-induced delayed type hypersensitivity (DTH) were studied in ICR male mice. SRBC were challenged by i.p. injection at two weeks after sensitization of Lp. injection of sRBC. Five days after the challenge of antigen, paw edema induced 24 hours after the last challenge by DTH, respectively. (omitted)

• Korean Journal of Veterinary Research
• /
• v.26 no.1
• /
• pp.149-156
• /
• 1986

An experiment was carried out to measure the cellular immune response in the animals by sensitizing the animals with 2, 4-dinitrochlorobenzene(DNCB). The fowls could be sensitized with primary application of DNCB. The sensitizing and challenge dose was standardized. Heterophils were prominent in the early inflammatory response in the superficial and deep dermal regions with scattering of eosinophils. The histological response in the fowls was characteristic of a delayed hypersensitivity reaction.

• Archives of Pharmacal Research
• /
• v.26 no.4
• /
• pp.312-316
• /
• 2003

The oriental herbal combination allergina has been shown to inhibit allergic inflammation. In the present study, we demonstrate that the oral administration of allergina markedly inhibits the progression of inflammatory diseases, such as graft-versus-host diseases (in the allogeneic bone marrow transplantation and the parent-into-F1 transplantation models), collagen-induced arthritis and sheep red blood cell-induced delayed type hypersensitivity. The immunosuppressive activity of allergina in vivo appears to be associated, at least in part, with the inhibition of tumor necrosis factor-a production. In conclusion, our results suggest that allergina could be useful as a immunosuppressive agent for the treatment of macrophage-related inflammatory disease.