• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.975-976
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• 2007

Thermal and mechanical stresses, caused by repetitive start and stop and load fluctuation during long time operation, on winding stator bars are one of the main causes for electrical degradation of insulating materials. To understand the degradation process, we manufactured bar specimens with the same processes that make generator winding stator bars and the specimens were subjected to various degrees of thermal cycling. Measurements of the insulation properties, such as dissipation factor, tip-up and partial discharge, for un-aged specimens and for specimens aged by thermal cycling at 50, 100, 250, 500 and 1000 thermal cycles were performed. Finally all specimens were tested to obtain electrical breakdown voltages. In this paper we present the data and electrical degradation analysis results obtained during this program.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2000년도 하계학술대회 논문집
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• pp.763-766
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• 2000

In this paper, stator form-winding sample coils based on silicone resin and polyimide were made for fault prediction and reliability estimation on the 200 Class insulation system of traction motors. The complex accelerative degradation was performed by periods during 10 cycles, which was composed of thermal stress, fast rising surge voltage, vibration, water immersion and overvoltage applying. After aging of 10 cycles, condition diagnosis test such as insulation resistance & polarization index, capacitance & dielectric loss and partial discharge properties were investigated in the temperature range of 20∼160$^{\circ}C$. Relationship among condition diagnosis test was analyzed to find an dominative degradation factor and an insulation state at end-life point.

• 한국신뢰성학회지:신뢰성응용연구
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• 제3권2호
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• pp.103-116
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• 2003

We carry out reliability tests and investigate the failure mechanisms. of the wafer level vacuum packaged MEMS gyroscope sensor using an accelerated degradation test. The accelerated degradation test (ADT) is used to evaluate reliability (and/or life) of the MEMS vacuum package and to select the accelerated test conditions, which reduce the reliability testing time. Using the failure distribution model and stress-life model, we are able to estimate the average life time of the vacuum package, which is well agreed with the measured data. After improving several package reliability issues such as prevention of gas diffusion through package, we carry out another set of accelerated tests at the chosen acceleration level. The results show that reliability of the vacuum packaged gyroscope has been greatly improved and can survive without degradation of performance, which is the Q-factor in gyroscope sensor, during environmental stress reliability tests.

• Macromolecular Research
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• 제9권4호
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• pp.210-219
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• 2001

Quenched and slow cooled as well as isothermally crystallized poly(tetramethylene succinate)(PTMS) films at two different temperatures were prepared. In the process of hydrolysis of the four specimens, structural changes such as the crystallinity, crystal size distribution, lattice parameter, lamellar thickness, long period and surface morphology were investigated by using wide and small angle X-ray scattering (WAXS and SAXS), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The hydrolytic degradation of quenched film was faster than that of slow cooled and isothermally crystallized films. The film crystallized at 100$\^{C}$ exhibited extensive micro voids and thus showed faster degradation than that crystallized at 75$\^{C}$, demonstrating surface morphology is another important factor to govern degradation rate. The crystallinity of the specimen increased by 5-10% and long period decreased after hydrolysis for 20 days. At the initial stage of degradation, the lamellar thickness of quenched film rather increased, while that of slow cooled and isothermally crystallized films decreased. The hydrolytic degradation preferentially occurred in the amorphous region. The hydrolytic degradation in crystal lamellae are mainly at the crystal surfaces.

• 한국신뢰성학회지:신뢰성응용연구
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• 제15권4호
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• pp.270-275
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• 2015

Purpose : UHMWPE (Ultra-high-molecular-weight-polyethylene) is one of the most widely used material in knife protection clothes because of high strength, elasticity, and light weight. The purpose of this study is to develop the accelerated life test method and predict the lifetime for the knife protection fabric composed by UHMWPE. Methods : In this study, degradation characteristics of UHMWPE fibers and knife protection fabric for cut resistance were evaluated under the hydrolysis and photo-degradation conditions. It was found out that the degradation rate of retained tensile strength was more significant in the photo-degradation than hydrolysis. Therefore, the failure time was determined as the time that the retained tensile strength in photo-degradation is less than 50%. Considering an acceleration factor for irradiance and exposure time, the lifetime was predicted from the calculated failure time. Results : As a result of the accelerated life test, the $B_{10}$ lifetime of knife protection fabric composed by UHMWPE fibers is estimated as 2.8 years for a 90% statistical confidence level. Conclusion: Since the lifetime is predicted by the view-point of radiant exposure in this study, there is a possibility that the estimated lifetime may differ from the actual lifetime. However, it is considered as an useful methodology to estimate the long-term lifetime of knife protection fabrics.

• BMB Reports
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• 제56권4호
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• pp.252-257
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• 2023

The hypoxia-inducible factor-1α (HIF-1α) is a key regulator of hypoxic stress under physiological and pathological conditions. HIF-1α protein stability is tightly regulated by the ubiquitin-proteasome system (UPS) and autophagy in normoxia, hypoxia, and the tumor environment to mediate the hypoxic response. However, the mechanisms of how the UPS and autophagy interplay for HIF-1α proteostasis remain unclear. Here, we found a HIF-1α species propionylated at lysine (K) 709 by p300/CREB binding protein (CBP). HIF-1α stability and the choice of degradation pathway were affected by HIF-1α propionylation. K709-propionylation prevented HIF-1α from degradation through the UPS, while activated chaperon-mediated autophagy (CMA) induced the degradation of propionylated and nonpropionylated HIF-1α. CMA contributed to HIF-1α degradation in both normoxia and hypoxia. Furthermore, the pan-cancer analysis showed that CMA had a significant positive correlation with the hypoxic signatures, whereas SIRT1, responsible for K709-depropionylation correlated negatively with them. Altogether, our results revealed a novel mechanism of HIF-1α distribution into two different degradation pathways.

• 한방안이비인후피부과학회지
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• 제21권3호
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• pp.82-93
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• 2008

Objective: Previously, the methanol extracts of the semen of Xanthium strumsrium could involved anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated Raw 264,7 cells, We evaluated the anti-allergic effects of X. strumarium on rat basophilic leukemia (RBL-2H3) cells, Methodes : To investigate the effect of X. strumarium on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced RBL-2H3 cells. The effects of X. strumarium on the degranulation and the pro-inflammatory cytokines secretion and expression from RBL-2H3 cells were evaluated with $\beta$-hexosaminidase assay, ELISA, and RT-PCR analysis, In addition, we examined the effects of X. strumarium on nuclear factor (NF)-${\kappa}B$ activation and $I{\kappa}B-\alpha$ degradation using Western blot analysis. Results : X. strumarium inhibited degranulation and secretions and expressions of pro-inflammatory cytokines, such as tumor necrosis factor-alpha ($TNF-\alpha$), interleukin (IL)-4 and cyclooxygenase (COX)-2, on stimulated RBL-2H3 cells, however, X. strumarium not affect cell viability. In stimulated RBL-2H3 cells, the protein expression level of nuclear factor-kappa B (NF-${\kappa}B$) was decreased in the nucleus by X. strumarium. In addition, X. strumarium suppressed the degradation of inhibitory protein $I{\kappa}B-{\alpha}$ protein in RBL-2H3 cells. Conclusion : These results suggest that X. strumarium inhibits the degranulation and secretion of pro-inflammatory cytokines through blockade of NF-${\kappa}B$ activation and I $I{\kappa}B-{\alpha}$ degradation.

• BMB Reports
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• 제38권2호
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• pp.167-176
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• 2005

Nuclear factor-E2-related factor 2 (Nrf2) is known as a key regulator of ARE-mediated gene expression and the induction of Phase II detoxifying enzymes and antioxidant enzymes, which is also a common property of many chemopreventive agents. In the present study, we investigated the regulatory role of different chemopreventive agents including sulforaphane (SUL), allyl isothiocyanate (AITC), indole-3-carbinol (I3C), and parthenolide (PTL), in the expression and degradation of Nrf2 and the induction of the antioxidant enzyme HO-1. SUL strongly induced Nrf2 protein expression and ARE-mediated transcription activation, retarded degradation of Nrf2 through inhibiting Keap1, and thereby activating the transcriptional expression of HO-1. AITC was also a potent inducer of Nrf2 protein expression, ARE-reporter gene and HO-1 but had little effect on delaying the degradation of Nrf2 protein. Although PTL and I3C could induce ARE reporter gene expression and Nrf2 to some extent, they were not as potent as SUL and AITC. However, PTL dramatically induced the HO-1 expression, which was comparable to SUL, while I3C had no effect. In addition, when treated with SUL and PTL, inhibition of proteasome by MG132 did not cause additional accumulation of Nrf2, suggesting the involvement of other degradation mechanism(s) in the presence of these compounds such as SUL and PTL. In summary, the results of our current study indicated that different chemopreventive compounds have different regulatory properties on the accumulation and degradation of Nrf2 as well as the induction of cellular antioxidant enzyme HO-1.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권1호
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• pp.171-192
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• 2017

The change of any element in the network is possible to cause performance degradation of the multicast network. So it is necessary to optimize the topology path through the multicast update strategy, which directly affects the performance and user experience of the overlay multicast. In view of the above, a new multicast tree update strategy based on perturbation theory Musp (Multicast Update Strategy based on Perturbation theory) is proposed, which reduces the data transmission interruption caused by the multicast tree update and improves user experiences. According to the multicast tree's elements performance and the topology structure, the Musp strategy defines the multicast metric matrix and based on the matrix perturbation theory it also defines the multicast fluctuation factor. Besides it also demonstrates the calculability of the multicast fluctuation factor presents the steps of the Musp algorithm and calculates the complexity. The experimental results show that compared with other update strategies, as for the sensitivity of the multicast fluctuation factor's energized multicast tree to the network disturbance, the maximum delay of the Musp update strategy is minimal in the case of the local degradation of network performance.

• BMB Reports
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• 제50권2호
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• pp.91-96
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• 2017

Nuclear factor erythroid 2-related factor 2 (Nrf2) provides a cellular defense against oxidative stress by inducing the expression of antioxidant and detoxification enzymes. The calcium antagonist, verapamil, is an FDA-approved drug prescribed for the treatment of hypertension. Here, we show that verapamil acts as a potent Nrf2 activator without causing cytotoxicity, through degradation of Kelch-like ECH-associated protein 1 (Keap1), a Nrf2 repressor. Furthermore, verapamil-induced Keap1 degradation is prominently mediated by a p62-dependent autophagic pathway. Correspondingly, verapamil protects cells from acetaminophen-induced oxidative damage through Nrf2 activation. These results demonstrated the underlying mechanisms for the protective role of verapamil against acetaminophen-induced cytotoxicity.