• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.26 no.2
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• pp.145-166
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• 2021

For several decades, sediment traps have served as one of the key tools for constraining the biological carbon pump (BCP), a process that vertically exports particulate organic carbon (POC) and associated biogenic materials from marine primary production in surface waters to the deep ocean interior. In this paper, I introduced the general methods, the current status of global sediment trap studies, and importance of it to understand the deep ocean carbon cycling. Recent studies suggest that sinking POC in the deep ocean are more complex and spatio-temporally heterogeneous than we considered. Especially researches those studied resuspended and laterally transported particles are presented. Researches that used organic (radiocarbon; ¹⁴C) and inorganic (Al) tracers to understand the oceanic POC cycling and the significance of resuspended particles are reviewed, and the importance of radiocarbon study by using MICADAS (Mini radioCarbon Dating Systems) is emphasized.

• Journal of Microbiology
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• v.43 no.2
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• pp.144-151
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• 2005

During the past ten years, Archaea have been recognized as a widespread and significant component of marine picoplankton assemblages. More recently, the presence of novel archaeal phylogenetic lineages has been discovered in coastal marine environments, freshwater lakes, polar seas, and deep-sea hydrothermal vents. Therefore, we conducted an investigation into the archaeal community existing in tidal flat sediment collected from Ganghwa Island, Korea. Phylogenetic analysis of archaeal 16S rDNA amplified directly from tidal flat sediment DNA revealed the presence of two major lineages, belonging to the Crenarchaeota ($53.9\%$) and Euryarchaeota ($46.1\%$) phyla. A total of 102 clones were then sequenced and analyzed by comprehensive phylogenetic analysis. The sequences determined in our samples were found to be closely related to the sequences of clones which had been previously obtained from a variety of marine environments. Archaeal clones exhibited higher similarities ($83.25 - 100\%$) to sequences..from other environments in the public database than did those ($75.22 - 98.46\%$) of previously reported bacterial clones obtained from tidal flat sediment. The results of our study suggest that the archaeal community in tidal flat sediment is remarkably diverse.

• International Journal of Ocean System Engineering
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• v.2 no.1
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• pp.1-7
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• 2012

The cross-shore sediment transport in a coastal region causes the important changes in beach morphological properties. The accurate estimation of the cross-shore sediment transport is important for the designing of the marine structures such as seawalls, jetties, breakwaters etc, and the preventing coastal erosion and accretion due to on-off shore sediment transportation. In this study, the experiments on cross- shore sediment transport carried out in a laboratory wave channel for initial beach slopes of 1/8, 1/10 and 1/15. Using the regular waves with different deep-water wave steepness generated by a pedal-type wave generator, the geometrical characteristics of beach profiles under storm conditions and the parameters affecting on-off shore sediment transport are investigated for the beach materials having medium diameters of $d_{50}$=0.25, 0.32, 0.45, 0.62 and 0.80 mm. The experimental results obtained from this study compared with previous experimental work and found to be of the same magnitude as the experimental measurements and followed the expected basic trend.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.952-958
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• 2004

Of 23 psychrotrophic bacteria isolated from the west Pacific deep-sea sediments, 19 were assigned to the $\gamma$-Proteobacteria, 3 to the <$\beta$-Proteobacteria, and 1 to the Gram-positive bacteria, as determined by their 16S rDNA sequences. Ten psychrotrophs, affiliated to the Psychrobacter, Pseudoalteromonas, and Pseudomonas genera in the $\gamma$-Proteobacteria group, were screened for lipolytic bacteria. The majority of the lipolytic isolates had growth temperatures between 4-$30^\circ{C}$, and all of them were neutrophilic, aerobic, or facultatively anaerobic, and some were able to produce multiple kinds of ectohydrolytic enzymes. The deep-sea strains Psychrobacter sp. wp37 and Pseudoalteromonas sp. wp27 were chosen for further lipase production analysis. Both strains had the highest lipase production when grown at 10 to $20^\circ{C}$; their highest lipase production occurred at the late-exponential growth stage; and the majority of the enzymes were excreted to the outside of the cells. Lipases from both strains had the same optimal reaction temperature and pH (20-$30^\circ{C}$, pH 7-8) and could retain about 60% of their highest activity at $4^\circ{C}$. Furthermore, SDS-PAGE and an in-gel activity test showed that they had the same high molecular mass of about 85 kDa.

• Proceedings of the Microbiological Society of Korea Conference
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• 2003.05a
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• pp.13-15
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• 2003

Studies on marine microbial diversity using direct analysis of rRNA sequences have revealed previously unrecognized microbes and novel phylogenetic lineages that represent major components of global microbial assemblages. This diversity in the marine biosphere offers opportunities for research and application in the field of biotechnology; global gas exchange, nutrient and element cycling, biomass md food production, marine bioproducts, and bioprocesses. Especially, deep-sea encompasses the extremes of virtually at] environmental parameters found on Earth and provides extreme microorganisms. In this study several extreme microorganisms were successfully isolated from the deep-sea sediment samples obtained by joining ocean cruises for last 2 years and some of them will be introduced.

• Korean Journal of Environmental Biology
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• v.41 no.4
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• pp.608-615
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• 2023

The morphological features of germling cells were examined to identify an unspecified resting cyst (described as Cochlodinium cf. polykrikoides-like resting cyst) in the Korean coastal area. LSU rRNA gene sequences were also obtained from a strain established from the germling cells. The resting cysts isolated from Korean coastal sediment were characterized as being brown in color, having a large dark-red body, and fibrous lobed ornaments. The germling cells were ellipsoidal with an irregular outline and had an open comma-shaped ASC (apical structure complex), a wide and deep cingulum, and a deep sulcus. These morphological features were consistent with those of previously described harmful dinoflagellate Pseudocochlodinium profundisulcus. The molecular phylogeny revealed that the germling cells and P. profundisulcus were conspecific. Based on these morphological and phylogenetic data, this study documents the occurrence of P. profundisulcus in a Korean coastal area for the first time.

• Journal of the Korean GEO-environmental Society
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• v.14 no.5
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• pp.33-39
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• 2013

A drilling exploration in deep sea is being processed to develop new energy resource in the world. In 2007, the presence of the gas hydrate had been confirmed during the UBGH1 (Ulleung Basin Gas Hydrate Expedition 1) in the Ulleung Basin. Geotechnical properties of the deep marine sediment are important factors for assessing the safety of gas production facility and productivity from the hydrate bearing sediment. In this study, comprehensive laboratory tests are conducted to investigate the geotechnical engineering characteristics of the deep marine sediments recovered from the hydrate occurrence regions during the UBGH2 (Ulleung Basin Gas Hydrate Expedition 2) in the Ulleung Basin, East Sea, Korea. The index properties of the specimens including the specific gravity, atterberg limits, specific surface, and particle size distribution are measured, and these are compared to the results reported by previous studies. A zero-lateral strain cell, which houses bender elements, is used to determine stress-dependant characteristics and shear wave velocities with the vertical effective stresses. Furthermore, the hydraulic conductivity is calculated based on the consolidation test results.

• Ocean and Polar Research
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• v.36 no.4
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• pp.407-421
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• 2014

The benthic environmental impact experiment addresses environmental impacts at a specific site related to deep-sea mineral resource development. We have acquired several tens of multi- or box core samples at 31 sites within the Benthic environmental Impact Site (BIS) since 2010, aiming to examine the basic properties of surficial deep-sea sediment as a potential source for deep-water plumes. In this study, we present the geochemical properties such as major elements, rare earth elements (REEs), and heavy metal contents at the BIS. Such proxies vary distinctly according to the Facies association. The lithology of all core sediments in the BIS corresponds to both Association Ib and Association IIIb. The vertical profiles of some major elements ($SiO_2$, $Fe_2O_3$, CaO, $P_2O_5$, MgO, MnO) show noticeable differences between Association Ib and IIIb, while others ($Al_2O_3$, $TiO_2$, $Na_2O$, and $K_2O$) do not vary between Association Ib and IIIb. REEs are also distinctly different for Associations Ib and IIIb; in Association Ib, REY and HREE/LREE are uniform through the sediment section, while they increase downward in Association IIIb like the major elements; below a depth of 8 cm, REY is over 500 ppm. The metal enrichment factor (EF) evaluates the anthropogenic influences of some metals (Cu, Ni, Pb, Zn, and Cd) in marine sediments. In both Associations, the EF for Cu is over 1.5, the EF for Ni and Pb ranges from 0.5 to 1.5, and the EF for Zn and Cd are less than 0.5, indicating Cu is enriched but Zn and Cd are relatively depleted in the BIS. The vertical variations of geochemical properties between Association Ib and IIIb are shown to be clearly different, which seems to be related to the global climate changes such as the shift of Intertropical convergence zone (ITCZ).

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.119-126
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• 2002

ATP concentrations far estimating total microbial biomass in the sediment were measured in three different marine sedimentary environments. ATP concentrations were highest in the surface sediment and decreased with increasing sediment depth and distance from the land. The results indicated that the benthic microbial biomass is primarily controlled by nutrient inputs from the overlying water column. Because of the longer residence time and adsorption to the sediment, the variations in organic carbon (OC) contents with sites and depths were not as distinct as that of ATP, and the correlation between OC and ATP was not significant in the coastal sediments. No significant correlation between OC and ATP in the coastal sediments also suggested that microbial biomass in the labile organic-enriched coastal sediment is suppressed by the grazing of higher trophic level such as meiofauna. Overall regional and vertical distribution of ATP indicated that h\`w can be a relevant tool for measuring total microbial biomass in various marine sedimentary environments.

• Journal of Ocean Engineering and Technology
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• v.30 no.5
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• pp.389-399
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• 2016

This paper presents the explorations of hydrothermal vents located in the Marina Arc and Back Arc Basin using the deep-sea ROV Hemire. These explorations were conducted by KRISO and KIOST to demonstrate the capability of Hemire in various applications for deep-sea scientific research. The missions included the following: (1) to search the reported vents, (2) conduct visual inspections, (3) deploy/recover a sediment trap and bait traps, (4) sample sediment/water/rock, (5) measure the magnetic field at the vent site, and (6) acquire a detailed map using multi-beam sonar near the bottom. We installed three HD cameras for precise visual inspection, a high-temperature thermometer, a three-component magnetometer, and a multi-beam sonar to acquire details of the bottom contour or identify vents in the survey area. The explorations were performed in an expedition from March 23 to April 5, 2016, and the missions were successfully completed. This paper discusses the operational process, navigation, and control of Hemire, as well as the exploration results.